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排序方式: 共有239条查询结果,搜索用时 250 毫秒
31.
E. M. Anokhin M. A. Popov I. V. Kochetov N. L. Aleksandrov A. Yu. Starikovskii 《Plasma Physics Reports》2016,42(1):59-67
Plasma decay in high-voltage nanosecond discharges in CO2: O2 and Ar: O2 mixtures at room gas temperature and a pressure of 10 Torr is studied experimentally and theoretically. The time dependence of the electron density during plasma decay is measured using microwave interferometry. The time evolution of the charged particle density, ion composition, and electron temperature is simulated numerically. It is shown that, under the given conditions, the discharge plasma is dominated for the most time by O 2 + ions and plasma decay is determined by dissociative and three-body electron?ion recombination. As in the previous studies performed for air and oxygen plasmas, agreement between measurements and calculations is achieved only under the assumption that the rate of three-body recombination of molecular ions is much greater than that for atomic ions. The values of the rate constant of three-body recombination of electrons with О2 + ions in a wide range of electron temperatures (500–5500 K), as well as for thermal (300 K) electrons, are obtained by processing the experimental results. 相似文献
32.
G.A. Kochetov N.K. Tikhomirova P.P. Philippov 《Biochemical and biophysical research communications》1975,63(4):924-930
The binding between thiamine pyrophosphate and transketolase, purified from baker's yeast, in equilibrium conditions has been studied. In the presence of Ca2+, the enzyme molecule has been shown to possess two binding sites for the coenzyme, whose dissociation constants are 3.2 × 10?8 and 2.5 × 10?7M; besides, there are site(s) where the binding of the coenzyme is less firm. In the presence of Mg2+, a positive cooperative interaction between the binding sites of thiamine pyrophosphate has been observed. Regardless of the cation used, the major part of the catalytic activity of the transketolase molecule manifests itself in the binding of one molecule of the coenzyme. 相似文献
33.
In the course of a freeze-cleave study on intercellular junctions in the regenerating rat liver, we observed an unusual array of intramembranous particles located in regions of contact between endothelial cells lining the hepatic sinusoids. These arrays were characterized by an accumulation of particles which resembled a zonula occludens in their linear deployment but differed in that the contact regions were composed of individual particles which remained separated from each other by regular particle-free intervals. 相似文献
34.
35.
A. N. Trushkin M. E. Grushin I. V. Kochetov N. I. Trushkin Yu. S. Akishev 《Plasma Physics Reports》2013,39(2):167-182
Results are presented from experimental studies of decomposition of toluene (C6H5CH3) in a polluted air flow by means of a steady-state atmospheric pressure glow discharge at different water vapor contents in the working gas. The experimental results on the degree of C6H5CH3 removal are compared with the results of computer simulations conducted in the framework of the developed kinetic model of plasma chemical decomposition of toluene in the N2: O2: H2O gas mixture. A substantial influence of the gas flow humidity on toluene decomposition in the atmospheric pressure glow discharge is demonstrated. The main mechanisms of the influence of humidity on C6H5CH3 decomposition are determined. The existence of two stages in the process of toluene removal, which differ in their duration and the intensity of plasma chemical decomposition of C6H5CH3 is established. Based on the results of computer simulations, the composition of the products of plasma chemical reactions at the output of the reactor is analyzed as a function of the specific energy deposition and gas flow humidity. The existence of a catalytic cycle in which hydroxyl radical OH acts a catalyst and which substantially accelerates the recombination of oxygen atoms and suppression of ozone generation when the plasma-forming gas contains water vapor is established. 相似文献
36.
37.
A homologous family of UDP- N -acetylgalactosamine: polypeptide N -
acetylgalactosaminyltransferases (GalNAc-transferases) initiate O-
glycosylation. These transferases share overall amino acid sequence
similarities of approximately 45-50%, but segments with higher similarities
of approximately 80% are found in the putative catalytic domain. Here we
have characterized the genomic organization of the coding regions of three
GalNAc-transferase genes and determined their chromosomal localization. The
coding regions of GALNT1 , -T2 , and -T3 were found to span 11, 16, and 10
exons, respectively. Several intron/exon boundaries were conserved within
the three genes. One conserved boundary was shared in a homologous C.
elegans GalNAc- transferase gene. Fluorescence in situ hybridization showed
that GALNT1 , -T2 , and -T3 are localized at chromosomes 18q12-q21,
1q41-q42, and 2q24-q31, respectively. These results suggest that the
members of the polypeptide GalNAc-transferase family diverged early in
evolution from a common ancestral gene through gene duplication.
相似文献
38.
39.
Russian Journal of Plant Physiology - Genetic engineering allows for an extension beyond the limits of a species' natural variability. This enables the production of ornamental plants with... 相似文献
40.
The interaction of thiamine diphosphate (ThDP) with transketolase (TK) involves at least two stages: [formula: see text] During the first stage, an inactive intermediate complex (TK...ThDP) is formed, which is then transformed into a catalytically active holoenzyme (TK* - ThDP). The second stage is related to conformational changes of the protein. In the preceding publication (Esakova, O. A., Meshalkina, L. E., Golbik, R., Hübner, G., and Kochetov, G. A. Eur. J. Biochem. 2004, 271, 4189 - 4194) we reported that the affinity of ThDP for TK considerably increases in the presence of the donor substrate, which may be a mechanism whereby the activity of the enzyme is regulated under the conditions of the coenzyme deficiency. Here, we demonstrate that the substrate affects the stage of the reverse conformational transition, characterized by the constant k(-1): in the presence of the substrate, its value is decreased several fold, whereas K(d) and k(+1) remain unchanged. 相似文献