Two Ochthephilus Mulsant and Rey species new to Korea (Coleoptera: Staphylinidae: Oxytelinae)

The genus Ochthephilus Mulsant and Rey and two species –O. antennatus (Watanabe and Shibata) and O. vulgaris (Watanabe and Shibata) – are identified for the first time in Korea. A key, illustrations of the habitus, and line drawings of diagnostic characters are presented.     

Two quediine species (Coleoptera: Staphylinidae: Staphylininae) new to Korea

Two quediine species (Quedius samuraicus Bernhauer and Schubert, and Velleius elongatus Naomi) were identified for the first time in Korea. Diagnoses and illustrations of the habitus and male genitalia are presented.     

Evidence for Symbiont-induced Alteration of a Host's Gene Expression: Irreversible Loss of SAM Synthetase from Amoeba proteus

ABSTRACT. Symbiont-bearing xD amoebae no longer produce a 45-kDa cytoplasmic protein that functions as S-adenosylmethionine synthetase in symbiont-free D amoebae. The absence of the protein in xD amoebae is attributable to xD amoeba's failure to transcribe the corresponding gene as a result of harboring bacterial symbionts. However, xD amoebae have about half the level of enzyme activity found in D amoebae, indicating that they use an alternative source for the enzyme. xD amoebae originated from D amoebae by bacterial infection and now depend on their symbionts for survival. xD amoebae exhibit irreversible nucleolar abnormalities when their symbionts are removed, suggesting that X-bacteria supply the needed enzyme. A monoclonal antibody against the 45-kDa protein was produced and used as a probe in cloning its corresponding cDNA. The product of the cDNA was found to have S-adenosylmethionine synthetase activity. These results show how symbiotic X-bacteria may become essential cellular components of amoebae by supplementing a genetic defect for an amoeba's house-keeping gene that is brought about by an action of X-bacteria themselves. This is the first reported example in which symbionts alter the host's gene expression to block the production of an essential protein.     

Phylogeny of the Myllaenini and related taxa (Coleoptera: Staphylinidae: Aleocharinae)

A cladistic analysis of the tribe Myllaenini Ganglbauer and related genera is presented. Monophyly of the Myllaenini is tested, and the tribe is hypothesized to be a monophyletic group consisting of nine genera (Myllaena Erichson, Amazonopora Pace, Dimonomera Cameron, Bryothinusa Casey, Philomina Blackwelder, Polypea Fauvel, Brachypronomaea Sawada, Rothium Moore and Legner, and Lautaea Sawada), based on the synapomorphy of antero‐lateral angles of mentum prolonged into spinose processes. A history of the classification of the Myllaenini is discussed. The data set for phylogenetic analysis comprised 99 characters representing 297 character states derived from adult morphology. The analysis agrees on the monophyly of the Myllaenini and the monophyly of the Pronomaeini Ganglbauer (Pronomaea Erichson, Pseudomniophila Pace, Nopromaea Cameron and Tomoxelia Bernhauer). The tribe Dimonomerini (Dimonomera Cameron) is confirmed to be a member of the Myllaenini. Masuriini is a possible sister group of the Myllaenini. Stylopalpus Cameron shows a sister group relationship to the Pronomaeini. Several other clades are also consistently recovered. However, the phylogenetic relationships of the genus Dysacrita are ambiguous. The rogue genus Diglotta Champion is not recovered as a member of the Myllaenini or Pronomaeini. On the contrary, it forms a monophyletic clade with the liparocephaline genera Halorhadinus Sawada and Amblopusa Casey. Evolution of the defensive gland on abdominal tergite VII among aleocharine lineages is reconsidered, and the origin of an intertidal habitat in the Myllaenini is discussed.     

Autalia rivularis (Gravenhorst) and Brachida clara (Weise) new to Korea (Coleoptera: Staphylinidae: Aleocharinae)

Two aleocharine species, Autalia rivularis (Gravenhorst) and Brachida clara (Weise), are identified for the first time in Korea. Diagnoses, habitus illustrations, and line drawings of diagnostic characters are provided.