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971.
Choi HS Lee JH Park JG Lee YI 《Biochemical and biophysical research communications》2002,296(4):1005-1012
972.
973.
974.
Kim JH Lee SY Bak SM Suh IB Lee SY Shin C Shim JJ In KH Kang KH Yoo SH 《American journal of physiology. Lung cellular and molecular physiology》2004,287(1):L127-L133
Bacterial infections of the lung are known to induce inflammatory responses, which lead to mucus hypersecretion. Moreover, mucin synthesis in the airways has been reported to be regulated by neutrophilic inflammation-induced epidermal growth factor receptor (EGFR) expression and its activation. Furthermore, matrix metalloproteinases (MMPs), especially MMP-9, have been reported to promote the transmigration of activated neutrophils. In this study, we investigated the associations between lipopolysaccharide (LPS)-induced goblet cell (GC) metaplasia and EGFR expression and the effects of MMP inhibitor (MMPI). Various concentrations of LPS were instilled into the tracheas of pathogen-free Sprague-Dawley rats, and airways were examined at different times after LPS instillation. To examine the role of MMP-9, we treated rats 3 days before LPS instillation and daily thereafter with MMPI. Neutrophilic infiltration, Alcian blue/periodic acid-Schiff (AB/PAS) staining, and immunohistochemical staining for MUC5AC, EGFR, and MMP-9 were performed. The instillation of LPS increased AB/PAS and MUC5AC staining in time- and dose-dependent manners, and treatment with MMPI significantly prevented GC metaplasia. The instillation of LPS into the trachea also induced neutrophilic infiltration and EGFR and MMP-9 expression in the airway epithelium, and MMPI was found to significantly prevent neutrophil recruitment, GC metaplasia, and EGFR and MMP-9 expression. This study demonstrates that the MMP-9 and EGFR cascades are associated with LPS-induced mucus hypersecretion. 相似文献
975.
976.
Baculovirus expression vectors that incorporate the foreign protein into viral occlusion bodies 总被引:4,自引:0,他引:4
Je YH Jin BR Park HW Roh JY Chang JH Seo SJ Olszewski JA O'Reilly DR Kang SK 《BioTechniques》2003,34(1):81-87
Current baculovirus expression systems typically produce soluble proteins that accumulate within the infected insect cell or are secreted into the growth medium. A system has now been developed for the incorporation of foreign proteins, along with the matrix protein, polyhedrin, into baculovirus occlusion bodies. Initial studies showed that a recombinant virus expressing a translational fusion between polyhedrin and GFP did not form occlusion bodies. However, a baculovirus coexpressing native polyhedrin and the polyhedrin-GFP fusion protein formed occlusion bodies that fluoresced under UV light, demonstrating that they included the polyhedrin-GFP fusion protein. This was confirmed by immunoblot analysis. Thus, incorporation of a foreign protein into occlusion bodies depends on an interaction between native polyhedrin and the polyhedrin fusion protein. Electron microscopy demonstrated that the occlusion bodies containing GFP also incorporated virions as expected. These ColorPol occlusion bodies were as infectious to insect larvae as occlusion bodies produced by wild-type virus. This new system expands the capabilities for foreign gene expression by baculoviruses, which has implications for biopesticide design, novel vaccine delivery systems, and fusion protein purification applications. 相似文献
977.
An improved baculovirus insecticide producing occlusion bodies that contain Bacillus thuringiensis insect toxin 总被引:5,自引:0,他引:5
Chang JH Choi JY Jin BR Roh JY Olszewski JA Seo SJ O'Reilly DR Je YH 《Journal of invertebrate pathology》2003,84(1):30-37
Baculovirus occlusion bodies, large proteinaceous structures which contain virions, have recently been engineered to incorporate foreign proteins. The major constituent protein of occlusion bodies from the baculovirus Autographa californica nucleopolyhedrovirus is polyhedrin, and assembly of recombinant occlusion bodies which incorporate a foreign protein depends on an interaction between native polyhedrin and a polyhedrin-foreign protein fusion. This technology has now been applied to the generation of a recombinant baculovirus (ColorBtrus) that produces occlusion bodies incorporating the Bacillus thuringiensis (Bt) insecticidal Cry1Ac toxin protein. ColorBtrus coexpresses native polyhedrin and a fusion protein in which polyhedrin is fused to the Bt toxin, which is in turn fused to green fluorescent protein (GFP). Analysis of ColorBtrus occlusion bodies confirmed that they include both Bt toxin and GFP, yet still incorporate virions. Bioassay of ColorBtrus demonstrated that its speed of action and pathogenicity are strikingly enhanced compared to wild-type virus. ColorBtrus represents a novel, powerful biological insecticide that combines positive attributes of both Bt toxin and baculovirus based systems. 相似文献
978.
Kim YC Zhao LX Kim TH Je SM Kim EK Choi H Chae WG Park M Choi J Jahng Y Lee ES 《Bioorganic & medicinal chemistry letters》2000,10(7):609-613
For the development of new anticonvulsive agents, gamma-vinyl GABA (vigabatrin) and GABA mimetics derivatives were covalently coupled as potential dual acting prodrugs and evaluated for their anticonvulsive activities. Among the prepared compounds, 11 showed the most potent anticonvulsive activity, a shorter onset time and a broader spectrum compared to vigabatrin. 相似文献
979.
Jezek P Zácková M Kosarová J Rodrigues ET Madeira VM Vicente JA 《Journal of bioenergetics and biomembranes》2000,32(6):549-561
The presence of plant-uncoupling mitochondrial protein (PUMP), previously described by Vercesi et al. (1995), was screened in mitochondria of various organs or tissues of several plant species. This was done functionally, by monitoring purine nucleotide-sensitive linoleic acid-induced uncoupling, or by Western blots. The following findings were established: (1) PUMP was found in most of the higher plants tested; (2) since ATP inhibition of linoleic acid-induced membrane potential decrease varied, PUMP content might differ in different plant tissues, as observed with mitochondria from maize roots, maize seeds, spinach leaves, wheat shoots, carrot roots, cauliflower, broccoli, maize shoots, turnip root, and potato calli. Western blots also indicated PUMP presence in oat shoots, carnation petals, onion bulbs, red beet root, green cabbage, and Sedum leaves. (3) PUMP was not detected in mushrooms. We conclude that PUMP is likely present in the mitochondria of organs and tissues of all higher plants. 相似文献
980.
探明咸水沟灌对土壤水稳性团聚体的影响对实现咸水资源安全高效利用具有重要意义。本研究在棉花长期定位咸水沟灌试验(始于2006年)的基础上,分析了不同矿化度(1、2、4、6、8、10 g·L-1)咸水连续灌溉第10年和第15年棉田土壤盐分和水稳性团聚体稳定性的变化特征。结果表明: 沟底0~30 cm土层土壤盐分随灌溉水矿化度的升高而增加,其中6、8、10 g·L-1灌水处理与1 g·L-1处理间差异达显著水平;随着土层深度的增加,各处理土壤盐分逐渐增大。咸水沟灌有降低沟底土壤水稳性团聚体稳定性的趋势,当灌溉水矿化度≥6 g·L-1时,水稳性大团聚体(>0.25 mm)质量分数、团聚体平均重量直径和几何平均直径显著降低,分形维数和平均重量比表面积显著增大;随着土层深度的增加,各处理土壤水稳性团聚体稳定性下降。连续多年咸水沟灌条件下,各处理棉田沟底0~30 cm土层土壤盐分和水稳性团聚体稳定性未随灌水年限的增加而逐年积累与恶化。在本研究灌溉制度下,采用≤4 g·L-1咸水沟灌对试验区棉田土壤盐分和水稳性团聚体稳定性指标无显著影响。 相似文献