A Secondary Alcohol Dehydrogenase from Tetrahymena furgasoni1

ABSTRACT. Dehydrogenase activity with hydroxysteroids has been observed in Tetrahymena furgasoni (formerly T. pyriformis strain W), and the enzyme responsible has been isolated from this organism. The purified dehydrogenase is active with a variety of steroid alcohols at apparent K_m values ranging from 0.2 to 4.0 mM. The C-3 hydroxyl of ring A of the steroid nucleus is the preferred position of oxidation. However, a variety of other secondary alcohols are also substrates, with apparent K_m values for 2-butanol, 2-pentanol, and cyclohexanol of 880, 1000. and 150 mM, respectively. With both steroidal and nonsteroidal alcohols. NAD is the preferred co-substrate, although low activity with NADP is observed. Evidence is presented that the activity with secondary alcohols, whether steroidal or not, is the property of a single protein species.     

The Effects of Nisin on the Microbial Flora of the Dental Plaque of Monkeys (Macaca fascicularis)

Nisin was incorporated in a soft diet and fed to eight monkeys for five months. At the end of this period dental plaque was removed from one site in the mouths of these animals and from a similar site in eight control animals. Twelve different selective media were used for the cultivation of the bacteria in the plaque. Streptococci, Haemophilus spp. and Gram negative anaerobes were the organisms most frequently isolated. There was some evidence that streptococci constituted a lesser proportion of the flora in the nisin-fed animals.     

The influence of hydrostatic pressure and pH on the rate of lysis of Micrococcus lysodeikticus by lysozyme

Under the conditions employed, the rate of clearing of washed cell suspensions of Micrococcus lysodeikticus, measured photometrically, is nearly proportional to enzyme concentration and nearly inversely proportional to the initial concentration of cells. With a given amount of lysozyme (usually 1.5 μg./ml.) the density of a cell suspension decreases exponentially with time, until it is reduced to between 20 and 40% of that at the start, followed by a decreasing rate of clearing. Under increased hydrostatic pressures of 4000 and 8000 p.s.i., respectively, the rate of clearing is faster than at normal pressure at various pH's between 5.65 and 8.70, at 35 °C.     

Temperature Dependence of Plant and Crop Process

The quantitative effects of temperature on plant and crop processesare discussed and related to the underlying theory, by meansof a critical and expository essay which aims to provide a conceptualbasis for the consideration of these problems. The topics consideredinclude the Arrhenius equation and Q₁₀; the collision and transition-statetheories of the rate constant; models with a temperature optimum;models without a temperature optimum; phase changes; diffusion,viscosity and translocation; hybrid systems (biochemistry plustransport); photosynsthesis; respiration; and development withthe day-degree hypothesis. Temperature, model, plants, crops     

Evidence that Enzyme Polymorphisms are not Selectively Neutral

THE discovery of large amounts of electrophoretically-detectable genetic variation within natural populations has aroused considerable interest in the factors maintaining so much polymorphism. It has been proposed that these allozyme polymorphisms reflect the action of random processes^1–6, the polymorphic variation being of little or no selective significance. Alternative hypotheses have suggested that these polymorphisms may be maintained by a balance of selective forces^7–10.     

A Model of the Partitioning of Growth between the Shoots and Roots of Vegetative Plants

A model of the partitioning of new growth between the shootsand roots of vegetative plants is presented. There are two partitioningfunctions, involving one partitioning parameter, which describethe priorities for new growth in both the shoots and roots.The dynamic responses, to changes in the environment and toshoot defoliation, of shoot and root specific growth rates,shoot: root ratio, and carbon and nitrogen substrate levels,are examined; realistic behaviour is observed. Balanced exponentialgrowth solutions are also examined and it is concluded thatrelationships between some derived plant growth quantities maybe non-unique, thus emphasizing the need for a critical understandingof the underlying physiological processes involved in plantgrowth. Mathematical model, partitioning of assimilates, shoot: root ratio, specific growth rate, carbon and nitrogen substrate levels     

Moulting cycles

Moulting in mammals is a cyclic phenomenon which often occurs in a wave–like pattern. The moult cycle depends upon an inherent rhythm of activity in the hair follicles, which may be modified by systemic factors. In laboratory rodents a number of hormones affect the timing of the moult, as well as affecting the amount of hair produced and the loss of club hairs.
In Microtus agrestis a seasonal moult results in a sparse coat with coarse hairs in summer and a dense coat with fine hairs in winter. The moult appears to be adjusted to the environment by way of the endocrine system, with adrenal and thyroid hormones, as well as sex hormones, involved in the regulation. The importance of such adaptive coat changes are discussed.