全文获取类型
收费全文 | 3408篇 |
免费 | 194篇 |
国内免费 | 1篇 |
出版年
2021年 | 30篇 |
2019年 | 25篇 |
2018年 | 27篇 |
2017年 | 25篇 |
2016年 | 48篇 |
2015年 | 79篇 |
2014年 | 111篇 |
2013年 | 207篇 |
2012年 | 149篇 |
2011年 | 174篇 |
2010年 | 80篇 |
2009年 | 89篇 |
2008年 | 157篇 |
2007年 | 166篇 |
2006年 | 154篇 |
2005年 | 135篇 |
2004年 | 142篇 |
2003年 | 153篇 |
2002年 | 162篇 |
2001年 | 126篇 |
2000年 | 131篇 |
1999年 | 124篇 |
1998年 | 49篇 |
1997年 | 39篇 |
1996年 | 27篇 |
1995年 | 29篇 |
1994年 | 32篇 |
1993年 | 31篇 |
1992年 | 76篇 |
1991年 | 76篇 |
1990年 | 63篇 |
1989年 | 74篇 |
1988年 | 61篇 |
1987年 | 39篇 |
1986年 | 42篇 |
1985年 | 49篇 |
1984年 | 39篇 |
1983年 | 48篇 |
1982年 | 19篇 |
1981年 | 19篇 |
1980年 | 18篇 |
1979年 | 36篇 |
1978年 | 22篇 |
1977年 | 16篇 |
1976年 | 19篇 |
1975年 | 19篇 |
1974年 | 13篇 |
1973年 | 18篇 |
1970年 | 14篇 |
1965年 | 13篇 |
排序方式: 共有3603条查询结果,搜索用时 15 毫秒
141.
Plasma membranes were isolated from rat liver mainly under isotonic conditions. As marker enzymes for the plasma membrane, 5'-nucleotidase and (Na+ + K+)-ATPase were used. The yield of plasma membrane was 0.6-0.9 mg protein per g wet weight of liver. The recovery of 5'-nucleotidase and (Na+ +K+)-ATPase activity was 18 and 48% of the total activity of the whole-liver homogenate, respectively. Judged from the activity of glucose-6-phosphatase and succinate dehydrogenase in the plasma membrane, and from the electron microscopic observation of it, the contamination by microsomes and mitochondria was very low. A further homogenization of the plasma membrane yielded two fractions, the light and heavy fractions, in a discontinuous sucrose gradient centrifugation. The light fraction showed higher specific activities of 5'-nucleotidase, alkaline phosphatase, (Na+ +K+)-ATPase and Mg2+-ATPase, whereas the heavy one showed a higher specific activity of adenylate cyclase. Ligation of the bile duct for 48 h decreased the specific activities of (Na2+ +K+)-ATPase and Mg2+-ATPase in the light fraction, whereas it had no significant influence on the activities of these enzymes in the heavy fraction. The specific activity of alkaline phosphate was elevated in both fractions by the obstruction of the bile flow. Electron microscopy on sections of the plasma membrane subfractions showed that the light fraction consisted of vesicles of various sizes and that the heavy fractions contained membrane sheets and paired membrane strips connected by junctional complexes, as well as vesicles. The origin of these two fractions is discussed and it is suggested that the light fraction was derived from the bile front of the liver cell surface and the heavy one contained the blood front and the lateral surface of it. 相似文献
142.
The binding constants of alpha- and beta-GlcNAc to hen and turkey lysozymes [EC 3.2.1.17] were determined at various pH's using the method proposed by Ikeda and Hamaguchi (1975) J. Biochem. 77, 1-16). The pH dependence of the binding of beta-GlcNAc to hen lysozyme was essentially the same as that for turkey lysozyme. The pH dependence curves of the binding constants of beta-GlcNAc to hen and turkey lysozymes were interpreted in terms of the participation of Glu 35 (pK 6.0), Asp 52 (pK 3.5), Asp 48 (pK 4.5), and Asp 66 (pK 1.5). The binding constants of alpha-GlcNAc to hen and turkey lysozymes were the same below pH 3.5 but were different above this pH. The main participant residues in the binding of alpha-GlcNAc were Glu 35, Asp 48, and Asp 66 for hen lysozyme and Glu 35 and Asp 66 for turkey lysozyme. The results obtained here were well explained by the following assumptions: (1) above about pH 4, alpha-GlcNAc binds to hen lysozyme in both alpha- and beta-modes, which correspond to the binding orientation of alpha-GlcNAc and that of beta-GlcNAc, respectively, as determined by X-ray crystallographic studies, but it binds predominantly in the beta-mode below about pH 4, (2) beta-GlcNAc binds to hen and turkey lysozymes predominantly in the beta-mode above about pH 4 and in both alpha- and beta-modes below pH 4, and (3) alpha-GlcNAc binds to turkey lysozyme predominantly in the beta-mode over the whole pH range studied. 相似文献
143.
Virulence of Streptococcus mutans: biochemical and pathogenic characteristics of mutant isolates. 总被引:23,自引:0,他引:23
S M Michalek T Shiota T Ikeda J M Navia J R McGhee 《Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.)》1975,150(2):498-502
The in vitro dextran-sucrase activities and adherence to glass of S. mutans 6715 and PS14 wild types and mutants were quantitated and compared with their in vivo cariogenicity in young, gnotobiotic rats. In general, S. mutans PS14 mutants B414 and B421 and 6715 mutant C4 demonstrated less dextran-sucrase activity and adherence than parental strains and caused fewer carious lesions in gnotobiotic rats. Rats monoinfected with either PS14 mutants B414 or B421 had less plaque and viable S. mutans in plaque than rats infected with parental strain. Both S. mutans 6715 mutants C211 and C229, demonstrated greater enzyme activity and adherence than the parental strain and produced more carious lesions. 相似文献
144.
Functional redundancy of multiple forest taxa along an elevational gradient: predicting the consequences of non‐random species loss 下载免费PDF全文
Akira S. Mori Takayuki Shiono Takashi F. Haraguchi Aino T. Ota Dai Koide Takayuki Ohgue Ryo Kitagawa Ryo Maeshiro Toe Toe Aung Taizo Nakamori Yusuke Hagiwara Shunsuke Matsuoka Anzu Ikeda Takuo Hishi Satoru Hobara Eri Mizumachi Andreas Frisch Göran Thor Saori Fujii Takashi Osono Lena Gustafsson 《Journal of Biogeography》2015,42(8):1383-1396
145.
146.
Yoshio Kawahara Tsuyoshi Ohsumi Yasuhiko Yoshihara Shigeho Ikeda 《Bioscience, biotechnology, and biochemistry》2013,77(9):2475-2479
Osmoregulation in Brevibacterium lactofermentum was studied. Proline was accumulated up to approximately 35mg/g dry cell weight in the cells of a wild strain of the bacterium grown under osmotic stress. The osmotic tolerance of a proline auxotroph mutant obtained from the bacterium was lower than that in the wild strain. The activity of pyrroline-5-carboxylate reductase, one of the enzymes in the proline biosynthetic pathway, increased about 3-fold when the cells of B. lactofermentum were grown under osmotic stress. These data indicated that proline is important in osmoregulation in the bacterium. 相似文献
147.
148.
149.
150.
Limnology - The in-stream processing of nutrients plays an important role in the fluvial nutrient transport from lands to the ocean, but few empirical studies have addressed the temporal dynamics... 相似文献