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51.
Soil water content, maize yield and its stability as affected by tillage and crop residue management in rainfed semi-arid highlands 总被引:3,自引:0,他引:3
Nele Verhulst Victoria Nelissen Niels Jespers Heleen Haven Ken D. Sayre Dirk Raes Jozef Deckers Bram Govaerts 《Plant and Soil》2011,344(1-2):73-85
Rainfed crop management systems need to be optimized to provide more resilient options to cope with projected climatic scenarios forecasting a decrease in mean precipitation and more frequent extreme drought periods in Mexico. Soil water content (0?C60 cm) was measured during three crop cycles in maize plots with different agronomic management practices in a long-term rainfed experiment (established in 1991) in the highlands of Mexico. Maize yields of 1997?C2009 were reported. Crop management practices varied in (1) tillage (conventional [CT] vs. zero tillage [ZT]) and (2) residue management (full or partial retention and removal). ZT with residue retention had higher soil water content than management practices involving CT and ZT with residue removal which provided a buffer for drought periods during the growing seasons. In 2009, a cycle with a prolonged drought during vegetative growth, this resulted in yield differences of up to 4.7 Mg ha?1 between ZT with (partial) residue retention and the other practices. Averaged over 1997?C2009, these practices had a yield advantage of approximately 1.5 Mg ha?1 over practices involving CT and ZT with residue removal. ZT with (partial) residue retention used rainfall more efficiently and resulted in a more resilient agronomic system than practices involving either CT or ZT with residue removal. 相似文献
52.
Marchal E Zhang J Badisco L Verlinden H Hult EF Van Wielendaele P Yagi KJ Tobe SS Vanden Broeck J 《Insect biochemistry and molecular biology》2011,41(4):219-227
Two genes coding for enzymes previously reported to be involved in the final steps of juvenile hormone (JH) biosynthesis in different insect species, were characterised in the desert locust, Schistocerca gregaria. Juvenile hormone acid O-methyltransferase (JHAMT) was previously described to catalyse the conversion of farnesoic acid (FA) and JH acid to their methyl esters, methyl farnesoate (MF) and JH respectively. A second gene, CYP15A1 was reported to encode a cytochrome P450 enzyme responsible for the epoxidation of MF to JH. Additionally, a third gene, FAMeT (originally reported to encode a farnesoic acid methyltransferase) was included in this study. Using q-RT-PCR, all three genes (JHAMT, CYP15A1 and FAMeT) were found to be primarily expressed in the CA of the desert locust, the main biosynthetic tissue of JH. An RNA interference approach was used to verify the orthologous function of these genes in S. gregaria. Knockdown of the three genes in adult animals followed by the radiochemical assay (RCA) for JH biosynthesis and release showed that SgJHAMT and SgCYP15A1 are responsible for synthesis of MF and JH respectively. Our experiments did not show any involvement of SgFAMeT in JH biosynthesis in the desert locust. Effective and selective inhibitors of SgJHAMT and SgCYP15A1 would likely represent selective biorational locust control agents. 相似文献
53.
Isolation and functional characterization of an allatotropin receptor from Manduca sexta 总被引:1,自引:0,他引:1
Horodyski FM Verlinden H Filkin N Vandersmissen HP Fleury C Reynolds SE Kai ZP Broeck JV 《Insect biochemistry and molecular biology》2011,41(10):804-814
Manduca sexta allatotropin (Manse-AT) is a multifunctional neuropeptide whose actions include the stimulation of juvenile hormone biosynthesis, myotropic stimulation, cardioacceleratory functions, and inhibition of active ion transport. Manse-AT is a member of a structurally related peptide family that is widely found in insects and also in other invertebrates. Its precise role depends on the insect species and developmental stage. In some lepidopteran insects including M. sexta, structurally-related AT-like (ATL) peptides can be derived from alternatively spliced mRNAs transcribed from the AT gene. We have isolated a cDNA for an AT receptor (ATR) from M. sexta by a PCR-based approach using the sequence of the ATR from Bombyx mori. The sequence of the M. sexta ATR is similar to several G protein-coupled receptors from other insect species and to the mammalian orexin receptor. We demonstrate that the M. sexta ATR expressed in vertebrate cell lines is activated in a dose-responsive manner by Manse-AT and each Manse-ATL peptide in the rank order ATL-I > ATL-II > ATL-III > AT, and functional analysis in multiple cell lines suggest that the receptor is coupled through elevated levels of Ca(2+) and cAMP. In feeding larvae, Manse-ATR mRNA is present at highest levels in the Malpighian tubules, followed by the midgut, hindgut, testes, and corpora allata, consistent with its action on multiple target tissues. In the adult corpora cardiaca--corpora allata complex, Manse-ATR mRNA is present at relatively low levels in both sexes. 相似文献
54.
55.
NADPH production by the pentose phosphate pathway in the zona fasciculata of rat adrenal gland. 总被引:1,自引:0,他引:1
Wilma M Frederiks Intan P E D Kümmerlin Klazina S Bosch Heleen Vreeling-Sindelárová Ard Jonker Cornelis J F Van Noorden 《The journal of histochemistry and cytochemistry》2007,55(9):975-980
Biosynthesis of steroid hormones in the cortex of the adrenal gland takes place in smooth endoplasmic reticulum and mitochondria and requires NADPH. Four enzymes produce NADPH: glucose-6-phosphate dehydrogenase (G6PD), the key regulatory enzyme of the pentose phosphate pathway, phosphogluconate dehydrogenase (PGD), the third enzyme of that pathway, malate dehydrogenase (MDH), and isocitrate dehydrogenase (ICDH). However, the contribution of each enzyme to NADPH production in the cortex of adrenal gland has not been established. Therefore, activity of G6PD, PGD, MDH, and ICDH was localized and quantified in rat adrenocortical tissue using metabolic mapping, image analysis, and electron microscopy. The four enzymes have similar localization patterns in adrenal gland with highest activities in the zona fasciculata of the cortex. G6PD activity was strongest, PGD, MDH, and ICDH activity was approximately 60%, 15%, and 7% of G6PD activity, respectively. The K(m) value of G6PD for glucose-6-phosphate was two times higher than the K(m) value of PGD for phosphogluconate. As a consequence, virtual flux rates through G6PD and PGD are largely similar. It is concluded that G6PD and PGD provide the major part of NADPH in adrenocortical cells. Their activity is localized in the cytoplasm associated with free ribosomes and membranes of the smooth endoplasmic reticulum, indicating that NADPH-demanding processes related to biosynthesis of steroid hormones take place at these sites. Complete inhibition of G6PD by androsterones suggests that there is feedback regulation of steroid hormone biosynthesis via G6PD. 相似文献
56.
The advent of web-based treatments for anxiety disorders creates a need for quick and valid online screening instruments, suitable for a range of social groups. This study validates a single-item multimedia screening instrument for agoraphobia, part of the Visual Screener for Common Mental Disorders (VS-CMD), and compares it with the text-based agoraphobia items of the PDSS-SR. The study concerned 85 subjects in an RCT of the effects of web-based therapy for panic symptoms. The VS-CMD item and items 4 and 5 of the PDSS-SR were validated by comparing scores to the outcomes of the CIDI diagnostic interview. Screening for agoraphobia was found moderately valid for both the multimedia item (sensitivity.81, specificity.66, AUC.734) and the text-based items (AUC.607-.697). Single-item multimedia screening for anxiety disorders should be further developed and tested in the general population and in patient, illiterate and immigrant samples. 相似文献
57.
M Chaki R Airik AK Ghosh RH Giles R Chen GG Slaats H Wang TW Hurd W Zhou A Cluckey HY Gee G Ramaswami CJ Hong BA Hamilton I Cervenka RS Ganji V Bryja HH Arts J van Reeuwijk MM Oud SJ Letteboer R Roepman H Husson O Ibraghimov-Beskrovnaya T Yasunaga G Walz L Eley JA Sayer B Schermer MC Liebau T Benzing S Le Corre I Drummond S Janssen SJ Allen S Natarajan JF O'Toole M Attanasio S Saunier C Antignac RK Koenekoop H Ren I Lopez A Nayir C Stoetzel H Dollfus R Massoudi JG Gleeson SP Andreoli DG Doherty 《Cell》2012,150(3):533-548
Nephronophthisis-related ciliopathies (NPHP-RC) are degenerative recessive diseases that affect kidney, retina, and brain. Genetic defects in NPHP gene products that localize to cilia and centrosomes defined them as "ciliopathies." However, disease mechanisms remain poorly understood. Here, we identify by whole-exome resequencing, mutations of MRE11, ZNF423, and CEP164 as causing NPHP-RC. All three genes function within the DNA damage response (DDR) pathway. We demonstrate that, upon induced DNA damage, the NPHP-RC proteins ZNF423, CEP164, and NPHP10 colocalize to nuclear foci positive for TIP60, known to activate ATM at sites?of DNA damage. We show that knockdown of CEP164 or ZNF423 causes sensitivity to DNA damaging agents and that cep164 knockdown in zebrafish results in dysregulated DDR and an NPHP-RC phenotype. Our findings link degenerative diseases of the kidney and retina, disorders of increasing prevalence, to mechanisms of DDR. PAPERFLICK: 相似文献
58.
CC2D2A is mutated in Joubert syndrome and interacts with the ciliopathy-associated basal body protein CEP290 总被引:1,自引:1,他引:0
Gorden NT Arts HH Parisi MA Coene KL Letteboer SJ van Beersum SE Mans DA Hikida A Eckert M Knutzen D Alswaid AF Ozyurek H Dibooglu S Otto EA Liu Y Davis EE Hutter CM Bammler TK Farin FM Dorschner M Topçu M Zackai EH Rosenthal P Owens KN Katsanis N Vincent JB Hildebrandt F Rubel EW Raible DW Knoers NV Chance PF Roepman R Moens CB Glass IA Doherty D 《American journal of human genetics》2008,83(5):559-571
Joubert syndrome and related disorders (JSRD) are primarily autosomal-recessive conditions characterized by hypotonia, ataxia, abnormal eye movements, and intellectual disability with a distinctive mid-hindbrain malformation. Variable features include retinal dystrophy, cystic kidney disease, and liver fibrosis. JSRD are included in the rapidly expanding group of disorders called ciliopathies, because all six gene products implicated in JSRD (NPHP1, AHI1, CEP290, RPGRIP1L, TMEM67, and ARL13B) function in the primary cilium/basal body organelle. By using homozygosity mapping in consanguineous families, we identify loss-of-function mutations in CC2D2A in JSRD patients with and without retinal, kidney, and liver disease. CC2D2A is expressed in all fetal and adult tissues tested. In ciliated cells, we observe localization of recombinant CC2D2A at the basal body and colocalization with CEP290, whose cognate gene is mutated in multiple hereditary ciliopathies. In addition, the proteins can physically interact in vitro, as shown by yeast two-hybrid and GST pull-down experiments. A nonsense mutation in the zebrafish CC2D2A ortholog (sentinel) results in pronephric cysts, a hallmark of ciliary dysfunction analogous to human cystic kidney disease. Knockdown of cep290 function in sentinel fish results in a synergistic pronephric cyst phenotype, revealing a genetic interaction between CC2D2A and CEP290 and implicating CC2D2A in cilium/basal body function. These observations extend the genetic spectrum of JSRD and provide a model system for studying extragenic modifiers in JSRD and other ciliopathies. 相似文献
59.
Caroline M. Docking Heleen A. Van de Weerd J.E.L. Day Sandra A. Edwards 《Applied animal behaviour science》2008,110(3-4):244-257
This experiment aimed to identify how a pig's age affected the extent and synchrony of use of different environmental enrichment materials, and how this use changed over time. Ten diverse novel objects were each presented to three replicate litters of 3 weeks of age (sucklers) and three replicate groups of three animals of 5 (weaners) weeks and 13 (growers) weeks of age. Video recordings were made of the pigs’ behaviour over a period of 5 days and subsequently analysed for activity, inactivity and object directed behaviour of three animals per group on days 1 and 5. The observed performance of any given behaviour, when at least one other member of the group was also performing that behaviour, was compared with the probability that such concurrence occurred by chance and these results were used to calculate the degree of synchronisation. Gender had no effect on the duration of object use or approach latency. Growers displayed a shorter latency to approach the objects initially compared to sucklers and weaners (mean seconds; 2153 versus 2660 versus 980 for sucklers, weaners and growers, respectively, S.E.D. 542, p = 0.007). Sucklers used the objects to a much lesser extent than either the weaners or growers (mean duration object interaction (s); 129 versus 1253 versus 1412 for sucklers, weaners and growers, respectively, S.E.D. 93, p < 0.001). Overall object use decreased between days 1 and 5 (mean duration object interaction (s); 1326 versus 536 for days 1 and 5, respectively, S.E.D. 76, p < 0.001). All of the age groups synchronised their behaviour to a much greater extent than expected by chance. The sucklers showed a higher degree of synchrony of activity (K; 0.890 versus 0.776 versus 0.682 for sucklers, weaners and growers respectively, S.E.D. 0.013, p < 0.001) and inactivity (K; 0.963 versus 0.905 versus 0.909 respectively, S.E.D. 0.007, p < 0.001), but lower degree of synchrony for object directed behaviour (K; 0.149 versus 0.375 versus 0.370 respectively, S.E.D. 0.025, p < 0.001), than the weaners and growers. The degree of synchronisation of object directed behaviour decreased over the 5-day period, irrespective of age (K; 0.382 versus 0.214 for days 1 and 5 respectively, S.E.D. 0.020, p < 0.001). Significant correlations (r = 0.678–0.879) were found between the degree of synchrony and extent of object interaction only for the sucklers. Since pigs showed behavioural synchronisation, object availability should be considered when providing desirable enrichment in order to avoid excessive competition in larger commercial groups. 相似文献
60.
Attema-de Jonge ME Bekkers JM Oudemans-van Straaten HM Sparidans RW Franssen EJ 《Journal of chromatography. B, Analytical technologies in the biomedical and life sciences》2008,862(1-2):257-262
After oral administration of tobramycin, as part of selective decontamination of the digestive tract (SDD) in critically ill patients, absorption of tobramycin from the gut into the blood may take place. To quantify low concentrations of tobramycin in human plasma, we developed and validated a simple (sample pre-treatment consisting of protein precipitation with acetonitrile using 200microl plasma), rapid (runtime 3min using a Pathfinder MR reversed-phase column) and sensitive (concentration range of 0.05-1.0mg/l using MS/MS detection) method. 相似文献