LINC00667 promotes Wilms’ tumor metastasis and stemness by sponging miR‐200b/c/429 family to regulate IKK‐β

Wilms' tumor, also known as nephroblastoma, is a kind of pediatric renal cancer. Previous studies have indicated that microRNAs (miRNAs) regulate various cancers progression. However, whether miR‐200 family regulated Wilms' tumor progression remains to be elucidated. In our study, miR‐200b/c/429 expression was downregulated in Wilms' tumor tissue samples from 25 patients. And data from three independent analyses of quantitative real‐time polymerase chain reaction revealed that the expression of miR‐200b/c/429 was downregulated in Wilms' tumor cell lines. Functionally, Cell counting kit‐8 assay revealed that cell viability was reduced by overexpressing miR‐200b/c/429. Transwell assay manifested that cell migration and invasion was hindered by miR‐200b/c/429 overexpression. Sphere‐forming and western blot assays demonstrated that miR‐200b/c/429 overexpression suppressed the sphere formation ability. Mechanically, nuclear factor‐κB (NF‐κB) pathway was confirmed to be associated with Wilms' tumor progression; miR‐200b/c/429 overexpression inactivated NF‐κB pathway as miR‐200b/c/429 was identified to target IκB kinase β (IKK‐β), an NF‐κB pathway‐related gene. Moreover, miR‐200b/c/429 was sponged by LINC00667 in Wilms' tumor cells. LINC00667 competitively bound with miR‐200b/c/429 to regulate IKK‐β expression and then activated NF‐κB pathway in Wilms' tumor. Subsequently, rescue assays illustrated that silencing of IKK‐β could reverse the effect of miR‐200b/c/429 inhibition on the progression of sh‐LINC00667‐transfected Wilms' tumor cells. In summary, LINC00667 promoted Wilms' tumor progression by sponging miR‐200b/c/429 family to regulate IKK‐β.     

Requirements for human embryonic stem cells

‘Requirements for Human Embryonic Stem Cells’ is the first set of guidelines on human embryonic stem cells in China, jointly drafted and agreed upon by experts from the Chinese Society for Stem Cell Research. This standard specifies the technical requirements, test methods, test regulations, instructions for use, labelling requirements, packaging requirements, storage requirements and transportation requirements for human embryonic stem cells, which is applicable to the quality control for human embryonic stem cells. It was originally released by the China Society for Cell Biology on 26 February 2019 and was further revised on 30 April 2020. We hope that publication of these guidelines will promote institutional establishment, acceptance and execution of proper protocols, and accelerate the international standardization of human embryonic stem cells for applications.     

基于中国生态系统研究网络的典型森林生态系统土壤保持功能分析

森林生态系统土壤保持功能在控制土壤侵蚀以及维持生态安全方面具有不可替代的作用。根据不同气候带降雨特征进行降雨侵蚀力参数校正,基于中国生态系统研究网络(CERN)的森林生态系统长期定位观测样地2005—2015年监测数据利用修正的通用土壤流失方程(RUSLE)定量分析了典型森林生态系统土壤保持功能的时空变化特征,并探讨了土壤保持功能的影响因素。研究结果表明:①日雨量侵蚀力模型在降雨丰富的热带模拟效果优于降雨相对较少的亚热带和温带,参数校正后模拟效果明显提升;②研究期内10个典型森林生态系统土壤保持量变化范围为4.44—891.67 t hm~(-2) a~(-1),呈现北低南高的空间格局(R~2=0.65~(***));土壤保持率均达到97%以上;③降雨、归一化植被指数、土壤质地和植被林龄是影响森林生态系统土壤保持功能的主要影响因素;降雨量与土壤保持量显著相关(R~2=0.52~*),NDVI和土壤质地与实际土壤侵蚀量显著相关(R~2=0.64~(**),R~2=0.41~*),植被林龄主要影响土壤保持率的变化速率。     

重点生态功能区农户的生计风险多维感知及影响因素——以甘南黄河水源补给区为例

重点生态功能区承担着保护与修复生态环境、提供生态产品的重要任务,农户作为该区最重要的生态保护主体,面临着多重风险的冲击,其对生计风险的多维感知不仅会影响其生产行为决策,更关系到重点生态功能区主体功能的发挥,因此,急需准确地了解农户的生计风险多维感知及影响因素,为制定有效的生计风险防范体系提供借鉴。以甘南黄河水源补给区为例,基于多维度生计风险感知测量框架,利用入户调查数据,分析了农户的生计风险感知,并采用经济计量模型分析了影响农户生计风险感知的关键因素。结果表明:①甘南黄河水源补给区农户面临的主要生计风险为家庭发展需求风险、健康风险、社会风险,且重点保护区农户面临环境风险、家庭发展需求风险、政策风险的比例均显著高于恢复治理区和经济示范区农户。②农户对家庭发展需求风险的熟悉性、自愿性、持续性感知均最高,对健康风险的恐慌性、严重性及可能性感知均最高,对社会风险的可控性感知最高。其中,重点保护区农户对政策、市场及健康风险的可控性感知均高于其他区域。③人力资本对可能性感知、严重性感知、恐慌性感知、熟悉性感知产生了显著影响;物质资本对自愿性感知产生了显著影响。最后,提出了农户防范生计风险的对策建议。     

Syntaxin-1通过激活突触递质传递加速早期突触的形成

Syntaxin-1是一种多结构域蛋白,通过与synaptobrevin-2和SNAP-25形成SNARE复合体调节囊泡融合.然而,syntaxin-1在突触形成过程中是否发挥作用,目前尚不清楚.本研究显示syntaxin-1的表达水平与突触形成过程高度相关.Syntaxin-1的R151A和I155A突变影响其在突触形成中的促进作用,而Habc结构域或跨膜结构域在突触形成中无显著作用.结果表明,syntaxin-1通过激活突触囊泡释放来加速突触的形成.