全文获取类型
收费全文 | 104篇 |
免费 | 16篇 |
出版年
2022年 | 2篇 |
2021年 | 3篇 |
2018年 | 6篇 |
2017年 | 4篇 |
2016年 | 7篇 |
2015年 | 4篇 |
2014年 | 4篇 |
2013年 | 7篇 |
2012年 | 13篇 |
2011年 | 4篇 |
2010年 | 7篇 |
2009年 | 1篇 |
2008年 | 2篇 |
2007年 | 3篇 |
2006年 | 5篇 |
2005年 | 3篇 |
2004年 | 3篇 |
2003年 | 6篇 |
2002年 | 3篇 |
2001年 | 1篇 |
2000年 | 1篇 |
1999年 | 1篇 |
1998年 | 1篇 |
1997年 | 3篇 |
1996年 | 2篇 |
1994年 | 2篇 |
1993年 | 3篇 |
1990年 | 8篇 |
1988年 | 4篇 |
1987年 | 1篇 |
1986年 | 4篇 |
1984年 | 1篇 |
1978年 | 1篇 |
排序方式: 共有120条查询结果,搜索用时 31 毫秒
11.
Tsedale Semunigus Belay Tessema Setegn Eshetie Feleke Moges 《Annals of clinical microbiology and antimicrobials》2016,15(1):50
Background
Tuberculosis (TB) remains one of the globe’s deadliest communicable diseases. The homeless individuals are at high risk to acquire TB and multi-drug resistant TB (MDR-TB), because of their poor living conditions and risky behaviors. Tuberculosis and MDR-TB in the homeless individuals can pose a risk to entire communities. However, the magnitude of the problem is not known in Ethiopia. Therefore, the aim of this study was to determine the prevalence and associated factors of smear positive pulmonary TB (PTB) and MDR-TB among homeless individuals in Dessie and Debre Birhan towns, Northeast Ethiopia.Methods
A community based cross-sectional study design was conducted from September 2014 to June 2015. Using an active screening with cough of ≥2 weeks, 351 TB suspects homeless individuals were participated in this study. Data were collected by using pre-tested and structured questionnaire. Spot-morning-spot sputum sample was collected and examined for acid-fast bacilli (AFB) using fluorescence microscopy by Auramine O staining technique. All AFB positive sputum was further analyzed by GeneXpert for detection of Mycobacterium tuberculosis complex and rifampicin resistant gene. Univariate and multivariate logistic regressions were applied to identify factors associated with smear positive PTB and P value <0.05 was considered as statistically significant.Results
The prevalence of smear positive PTB was 2.6 % (95 % CI 1.3–5) among TB suspect homeless individuals. Extrapolation of this study finding implies that there were 505 smear positive PTB per 100,000 homeless individuals. All smear positive PTB sputum specimens were further analyzed by GeneXpert assay, the assay confirmed that all were positive for MTBC but none were resistant to RIF or MDR. Smoking cigarette regularly for greater than 5 years (AOR 10.1, 95 % CI 1.1, 97.7), body mass index lower than 18.5 (AOR 6.9, 95 % CI 1.12, 41.1) and HIV infection (AOR 6.8, 95 % CI 1.1, 40.1) were significantly associated with smear positive PTB.Conclusion
The prevalence of smear positive PTB among TB suspect homeless individuals was 2.6 %. Among smear positive PTB, prevalence of HIV co-infection was very high 5 (55.5 %). Smoking cigarette regularly for greater than 5 years, BMI lower than 18.5 and HIV infection were factors associated with smear positive PTB. Special emphasis is needed for homeless individuals to exert intensive effort to identify undetected TB cases to limit the circulation of the disease into the community.12.
Effects of solvent polarity on the absorption and fluorescence spectra of chlorogenic acid and caffeic acid compounds: determination of the dipole moments 下载免费PDF全文
The effects of solvent polarity on absorption and fluorescence spectra of biologically active compounds (chlorogenic acid (CGA) and caffeic acids (CA)) have been investigated. In both spectra pronounced solvatochromic effects were observed with shift of emission peaks larger than the corresponding UV‐vis electronic absorption spectra. From solvatochromic theory the ground and excited‐state dipole moments were determined experimentally and theoretically. The differences between the excited and ground state dipole moment determined by Bakhshiev, Kawski–Chamma–Viallet and Reichardt equations are quite similar. The ground and excited‐state dipole moments were determined by theoretical quantum chemical calculation using density function theory (DFT) method (Gaussian 09) and were also similar to the experimental results. The HOMO‐LUMO energy band gaps for CGA and CFA were calculated and found to be 4.1119 and 1.8732 eV respectively. The results also indicated the CGA molecule is more stable than that of CFA. It was also observed that in both compounds the excited state possesses a higher dipole moment than that of the ground state. This confirms that the excited state of the hydroxycinnamic compounds is more polarized than that of the ground state and therefore is more sensitive to the solvent. Copyright © 2015 John Wiley & Sons, Ltd. 相似文献
13.
The purpose of this study was to examine the effects of catecholamines on in vitro growth of a range of bacterial species, including anaerobes. Bacteria tested included: Porphyromonas gingivalis, Bacteriodes fragilis, Shigella boydii, Shigella sonnie, Enterobacter Sp, and Salmonella choleraesuis. The results of the current study indicated that supplementation of bacterial cultures in minimal medium with norepinephrine or epinephrine did not result in increased growth of bacteria. Positive controls involving treatment of Escherichia coli with catecholamines did result in increased growth of that bacterial species. The results of the present study extend previous observations that showed differential capability of catecholamines to enhance bacterial growth in vitro. 相似文献
14.
Gu Ji-Dong Belay Brook Mitchell Ralph 《World journal of microbiology & biotechnology》2001,17(2):173-179
A catheter surface was modified by coating a cellulose acetate polymer. Adhesion of Pseudomonas aeruginosa ATCC 27853 to the surface was investigated by exposing bacterial cultures to three treatments: polymer impregnated with silver ions (Ag+), polymer surfaces coated with lectins and a combination of Ag+ and a lectin coating. The effective concentration of Ag+ providing protection against bacterial biofilm development was 100g/ml and higher. Lectins alone at 10% also showed inhibition of bacterial attachment. However, the best result was achieved against bacterial adhesion and growth on surfaces using a combination of 100 g Ag+/ml and a lectin coating as a surface treatment. This surface treatment was also effective against both fresh culture and a two-week-old culture containing P. aeruginosa producing exopolymers. Our results suggest that Ag+impregnation combined with a lectin coating warrants further investigation as a potential means of protecting catheters. 相似文献
15.
In 1989 a new gelada baboon (Theropithecus gelada) population was found in Arsi, on the opposite side of the Rift Valley to that of the known gelada populations of Semien
and Showa. Previous comparisons of units of the band at Gado Goro, Arsi, in the same season in consecutive years, indicated
that unit structure is less stable among Arsi geladas as compared to the Semien population. Gelada units of the band at Gado-Goro
were studied for 7 months in order to investigate the processes of social changes. Changes in unit structure were observed.
Provisioning was carried out for 1.5 months at the beginning of the 7-month study period, in order to capture and obtain blood
samples from the geladas. Following this, changes in male leadership of some units were observed, presumably as a consequence
of the capture. However, natural changes also occurred. One change in unit structure occurred after a female gave birth, and
changes in another unit occurred after the disappearance of the leader male. These changes involved female desertion of a
unit, her subsequent transfer to a male unit, and culminated in the formation of a unit consisting of one female and one male.
One successful and one attempted case of unification of units, and one case of change of a unit leader male are reported.
These changes occurred among eight resident units in a period of 7 months (196 female months). Though the types of social
changes were not much different from previous observations in Semien National Park, their frequencies seemed to be much higher.
The characteristics of Arsi gelada social changes are proposed to be related to the small size of the units. We also describe
a new confirmed case and one suspected case of infanticide, as well as one case of abortion at the time of male leader change. 相似文献
16.
Mulugeta Belay Mengistu Legesse Adane Mihret Yonas Bekele Tom H. M. Ottenhoff Kees L. M. C. Franken Gunnar Bjune Fekadu Abebe 《PloS one》2015,10(4)
Tuberculosis (TB) is among the leading causes of morbidity and mortality. The causative agent, Mycobacterium tuberculosis (Mtb), has evolved virulent factors for entry, survival, multiplication and immune evasion. Rv2031 (also called alpha crystallin, hspX, 16-kDa antigen), one of the most immunogenic latency antigens, is believed to play a key role in long-term viability of Mtb. Here, we report the dynamics of pro-inflammatory (IFN-γ, TNF-α) and anti-inflammatory (IL-10) cytokines against Rv2031 using whole blood assay in human cohorts in a TB endemic setting. Cytokine responses to ESAT-6-CFP-10 were also measured for comparison. Blood samples were collected from smear positive pulmonary TB patients and their contacts at baseline, 6 and 12 months, and from community controls at entry. At baseline, 54.4% of controls and 73.2% of contacts were QFT-GIT test positive. Baseline IFN-γ, TNF-α and IL-10 responses to Rv2031 were significantly higher in controls compared to contacts and untreated patients (p<0.001). Furthermore, untreated patients had significantly higher TNF-α and IL-10 responses to Rv2031 compared to contacts (p<0.001). In contacts and treated patients, IFN-γ, TNF-α and IL-10 responses to Rv2031 significantly increased over 12 months (p<0.0001) and became comparable with the corresponding levels in controls. There was a positive and significant correlation between Rv2031 and ESAT-6-CFP-10 specific cytokine responses in each study group. The fact that the levels of IFN-γ, TNF-α and IL-10 against Rv2031 were highest during latent TB infection may indicate their potential as markers of protection against TB. Taken together, the findings of this study suggest the potential of IFN-γ, TNF-α and IL-10 against Rv2031 as biomarkers of the host response to Mtb during convalescence from, and the absence of, active tuberculosis. 相似文献
17.
Changes of external morphological characters in three forms (morphotypes) of African barbs from Lake Tana (Ethiopia) belonging
to Barbus intermedius complex were traced using electronic tags. It was shown that within age interval from two to four years individual ontogenetic
allometric curves often cannot be described with a single power function equation. Individual ontogenetic trajectories in
the principal components space within this interval strongly differ in shape meandering within ontogenetic channels outlined
on the basis of cross-sectional data. Morphological differences between siblings are found that do not allow to classify some
of them with the morphotype of their parents. 相似文献
18.
K. F. Dzerzhinskii F. N. Shkil Belay Abdissa Wondie Zelalem M. V. Mina 《Journal of Ichthyology》2007,47(8):639-646
Direct observations on three forms (morph types) of spawning barbs, considered by some authors as different species, were
carried out in September–October 2005 at the Gumara River and its small tributary, the Ducalit, in the Lake Tana basin. The
spawning barbs were sampled using cast nets. Barbs of different morphotypes composed common spawning aggregations and were
caught together. It is likely that troutlike females mate extensively with intermedius males and, probably, with bigmouth
small-eye males. Experiments in artificial fertilization and rearing showed that, during the embryonic period, there was no
increase in mortality of progeny from crosses of barbs of different morphotypes, in comparison with homonomic crosses, thus
suggesting an absence of postzygotic reproductive isolation between the morphotypes. At the same time, mortality of progeny
from crossing barbs with Varicorhinus beso Rüppell, 1836 was very high during the earliest stages of development, suggesting strong, even if incomplete, reproductive
isolation ensured by postzygotic mechanisms.
Published in Voprosy Ikhtiologii, 2007, Vol. 47, No. 5, pp. 676–683.
The article was translated by the authors. 相似文献
19.
Daniel Fitamo Seyoum Leta Gurja Belay Bekele Lemma Mats Olsson 《Soil & Sediment Contamination》2011,20(7):745-766
Irrigation with untreated wastewater from several industrial, commercial, and domestic discharges for decades caused accumulation of various heavy metals and metalloids in soils along the Akaki River in Ethiopia. Assessment of environmental threats and the potential phytoremediation of the soils require understanding of the toxic elements’ uptake and distribution in plant parts. Hence, a greenhouse study was performed to examine the phytoavailability and distribution of Cr, Ni, Co, Cu, Zn, Cd, Pb, Hg, Se, V, and As in forage grasses: Oat (Avena sativa), Rhodes grass (Chloris gayana), Setaria (Setaria sphacelata), and the legumes Alfalfa (Medicago sativa) and Desmodium (Desmodium unicinatum). The average contents of Cr, Ni, Co, Cu, Zn, Pb, Hg, Se, and V in the plants were generally higher than the background levels for forage grasses/legumes, and some of these elements were in the phytotoxic range. Root bioconcentration factor (BCF = root to soil concentration ratio) > 1 was observed for Cu (Oat, Rhodes, Desmodium, and Setaria: Fluvisol), Zn (Setaria: Fluvisol), Cd (Rhodes: Fluvisol; Setaria from both soils) and Hg (Oat and Alfalfa: Fluvisol). Alfalfa and Desmodium displayed translocation factor > 1 (TF = shoot to root concentration ratio) for most heavy metals. Most heavy metals/metalloids may pose a health threat to humans and stock via introduction to the food chain. The plant factors (species and plant part), soil factors (soil type, soil fractions, pH, and CEC), and their interactions significantly (p < 0.05) influenced plant heavy metal and metalloid levels. However, the role of plant part and species emerged as the most important on heavy metal uptake, translocation, sequestration, and ultimately transfer to the food chain. Accordingly, the uptake and distribution of heavy metals/metalloids in the plants reflect the potential environmental and health hazards attributable to the use of fodder grasses, legumes, and cultivation of vegetables in soils with polymetallic and metalloid contamination. 相似文献
20.
Denis Baurain Laurent Renquin Stana Grubisic Patsy Scheldeman Amha Belay Annick Wilmotte 《Journal of phycology》2002,38(2):384-393
The internally transcribed spacer (ITS) sequences of 21 Arthrospira clonal strains from four continents and assigned to four different species (A. platensis, A. maxima, A. fusiformis, A. indica) in the culture collections were determined. Two main clusters, I and II, were differentiated by 49 positions out of 475 nt or 477 nt, respectively. Each cluster was further subdivided into two subclusters. Subclusters I.A and I.B were separated by two substitutions, whereas subclusters II.A and II.B were distinguished by four substitutions. After direct sequencing of the PCR products, three dried samples from Chad aged between 3 months and 35 years yielded a sequence belonging to subcluster I.A, as did a recent commercial product. The strains grown in production plants belonged to the same (sub)clusters as strains from culture collections, mainly I.A and II. PCR primers specific for each cluster and subcluster were designed and tested with crude cell lysates of Arthrospira strains. One dried sample (“dihé” 1) and a herbarium sample from Lake Sonachi (Kenya) only contained I.A sequences, whereas the commercial product was a mixture of the four genotypes and the other two dried samples contained minor polymorphisms characteristic of different clusters. Five clonal Arthrospira strains, thought to be duplicates, showed the simultaneous presence of the two forms of the four diagnostic positions that distinguish subclusters genotype II.A and genotype II.B. This is likely to be caused by multiple copies of the rDNA operon, in a intermediate stage of homogenization between subcluster II.A and subcluster II.B. The high conservation of ITS sequences is in contrast with the assignment to four different species, the great morphological variability of the strains, and their wide geographic distribution. 相似文献