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41.
Nkoghe D Kassa RF Caron M Grard G Mombo I Bikié B Paupy C Becquart P Bisvigou U Leroy EM 《PLoS neglected tropical diseases》2012,6(2):e1517
Background
Chikungunya virus (CHIKV) has caused multiple outbreaks in tropical and temperate areas worldwide, but the clinical and biological features of this disease are poorly described, particularly in Africa. We report a prospective study of clinical and biological features during an outbreak that occurred in Franceville, Gabon in 2010.Methodology/Principal Findings
We collected, in suspect cases (individuals presenting with at least one of the following symptoms or signs: fever, arthralgias, myalgias, headaches, rash, fatigue, nausea, vomiting, diarrhea, bleeding, or jaundice), blood samples, demographic and clinical characteristics and outcome. Hematological and biochemical tests, blood smears for malaria parasites and quantitative PCR for CHIKV then dengue virus were performed. CHIKV+ patients with concomitant malaria and/or dengue were excluded from the study. From May to July 2010, data on 270 laboratory-confirmed CHIK patients were recorded. Fever and arthralgias were reported by respectively 85% and 90% of patients, while myalgias, rash and hemorrhage were noted in 73%, 42% and 2% of patients. The patients were grouped into 4 clinical categories depending on the existence of fever and/or joint pain. On this basis, mixed forms accounted for 78.5% of cases, arthralgic forms 12.6%, febrile forms 6.7% and unusual forms (without fever and arthralgias) 2.2%. No cases of organ failure or death were reported. Elevated liver enzyme and creatinine levels, anemia and lymphocytopenia were the predominant biological abnormalities, and lymphocytopenia was more severe in patients with high viral loads (p = 0.01).Conclusions/Significance
During CHIK epidemics, some patients may not have classical symptoms. The existence of unusual forms and the absence of severe forms of CHIK call for surveillance to detect any change in pathogenicity. 相似文献42.
Free energies for stacking of unpaired nucleotides (dangling ends) at the termini of oligoribonucleotide Watson-Crick helixes (DeltaG(0)37,stack) depend on sequence for 3' ends but are always small for 5' ends. Here, these free energies are correlated with stacking at helix termini in a database of 34 RNA structures determined by X-ray crystallography and NMR spectroscopy. Stacking involving GA pairs is considered separately. A base is categorized as stacked by its distance from (=4.0 A), angle with (=30 degrees), and overlap with the terminal helix base-pair. A base is unstacked if it does not satisfy one or more of these criteria.Of the 36 unpaired bases in sequences with DeltaG(0)37,stackmore favorable than -0.7 kcal/mol, 30 (83 %) are stacked on the adjacent base-pair, indicating a propensity for such sequences to stack in the 3D structure. Structures containing the strongly stacked sequence [sequence: see text] show that the amino group of C closely overlaps the carbonyl-4 of U. Thermodynamic measurement of U stacking on a 2-pyrimidinone-guanine base-pair, where the amino group of C is replaced by hydrogen, suggests that interactions with the cytosine amino group contribute approximately 0.5 kcal/mol to DeltaG(0)37,stack.For GA mismatches at helix termini, the nucleotide at the 3' helix end is always stacked, and the nucleotide at the 5' end is stacked in almost 90 % of occurrences. In available structures, non-Watson-Crick paired bases 3' to an imino-hydrogen bonded GA are also always stacked; the GA provides a large platform for favorable stacking.For the 56 sequences associated with DeltaG(0)37,stackless favorable than -0.4 kcal/mol, 19 (34 %) are stacked; these sequences have a propensity for not stacking on adjacent base-pairs. Phylogenetic conservation of weakly stacking sequences at 3' ends may be a predictor of a backbone turn. 相似文献
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The O-linked oligosaccharides of the jelly coat surroundingthe eggs of Xenopus laevis were analysed by 1H-NMR spectroscopy.Among the 12 neutral oligosaccharide-alditols which have beencharacterized, three of them posses the following unusual structures,As previously observed for six other amphibian species, thecarbohydrate chains of the jelly coat of Xenopus eggs displaya high species specificity which could support a biologicalrole during the fertilization processes. amphibian egg jelly coats 1H-NMR oligosaccharide structure Xenopus laevis 相似文献
45.
通过差别筛选HgCl2胁迫下的菜豆叶片cDNA库,分离出7组不同的cDNA克隆(Phaseolusvulgarisstress-relatedprotein,PvSR1~7)。cDNA序列和同源性分析结果表明:PvSR1编码富含脯氨酸细胞壁蛋白(PRP),PvSR2和PvSR7编码新的HgCl2胁迫相关蛋白,PvSR3编码脱水蛋白(dehydrin),PvSR4编码病原相关(PR)蛋白,PvSB5编码polyubiqui-tin,PvSR6编码DuaJ-like蛋白。HgCl2胁迫可强烈地请导PvSR2和PR蛋白基因的表达,并能提高PRP,、dehydrinlike和polyubiq-uitin基因的转录水平。这些蛋白质共同作用可能对维持细胞的正常代谢和抵抗重金属胁迫方面有重要作用。 相似文献
46.
Isolation of a complementary DNA encoding the bean PR4 chitinase: an acidic enzyme with an amino-terminus cysteine-rich domain 总被引:7,自引:0,他引:7
Marcia Margis-Pinheiro Marie Hélène Metz-Boutigue Abalo Awade Marc de Tapia Monique le Ret Géraud Burkard 《Plant molecular biology》1991,17(2):243-253
The amino acid sequences of peptides generated by trypsin and chymotrypsin digestions of the acidic PR4 chitinase from bean were determined. Oligonucleotide primers derived from this sequence were used to synthesize a PR4 chitinase-specific probe by PCR-amplification. This probe allowed the isolation of cDNA clones encoding PR4 chitinase that have been sequenced. This acidic and extracellular chitinase shows some homology to the basic isoform from the same plant, and differs from other known acidic chitinases by the presence of an amino-terminal cysteine-rich domain. Southern blot analysis of bean genomic DNA revealed that PR4 chitinase is encoded by a single gene. 相似文献
47.
Elif Nurtop Paola Mariela Saba Villarroel Boris Pastorino Laetitia Ninove Jan-Felix Drexler Yelin Roca Bouba Gake Audrey Dubot-Peres Gilda Grard Christophe Peyrefitte Stéphane Priet Xavier de Lamballerie Pierre Gallian 《Virology journal》2018,15(1):192
Here we propose a strategy allowing implementing efficient and practicable large-scale seroepidemiological studies for Zika Virus (ZIKV). It combines screening by a commercial NS1 protein-based Zika IgG ELISA, and confirmation by a cytopathic effect-based virus neutralization test (CPE-based VNT). In post-epidemic samples from Martinique Island blood donors (a population with a dengue seroprevalence above 90%), this strategy allowed reaching specificity and sensitivity values over 98%. The CPE-based VNT consists of recording CPE directly under the optical microscope, which is easy to identify with ZIKV strain H/PF/2013 at day 5 pi. Overall, considered that CPE-based VNT is cost effective and widely automatable, the NS1 protein-based Zika IgG ELISA+CPE-based VNT combination strategy represents a convenient tool to expedite ZIKV seroprevalence studies. 相似文献
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In our study, we investigated whether multiple fluorescence indices may be used to sense physiological changes in tomato plants (Solanum lycopersicum L.) caused by salinity and water deficit as single or combined stresses. The fluorescence intensity in the blue (B), red (R) and far-red (FR) spectral regions and the pulse-amplitude-modulated (PAM) chlorophyll fluorescence, were recorded on a weekly basis in the scope of a long-term experiment. The results indicate the coefficient of photochemical quenching (qL), the B to FR fluorescence ratio and the logarithm of the FR fluorescence ratio after R and UV-light excitation as appropriate parameters to sense the response of plants to the imposed stress. The qL revealed the impact of water deficiency, whereas the two multispectral ratios revealed the influence of combined salinity and water shortage. Despite minor changes in the chlorophyll concentration, salinity and water deficit, when combined, had an additive impact on the chlorophyll fluorescence. Overall, the fluorescence signals of ‘Rio Grande’ were more affected by the induced stresses compared to ‘Harzfeuer’. The multiparametric fluorescence technique, confirming the trends obtained with the PAM-method, reveals promising perspectives for the ‘in situ’ evaluation of the physiological status of horticultural crops. 相似文献