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991.
Silvia De Francia Antonio D’Avolio Francesca De Martino Elisa Pirro Lorena Baietto Marco Siccardi Marco Simiele Silvia Racca Giuseppe Saglio Francesco Di Carlo Giovanni Di Perri 《Journal of chromatography. B, Analytical technologies in the biomedical and life sciences》2009,877(18-19):1721-1726
A new method using high performance liquid chromatography coupled with electrospray mass spectrometry is described for the quantification of plasma concentration of tyrosine kinase inhibitors imatinib, dasatinib and nilotinib. A simple protein precipitation extraction procedure was applied on 250 μl of plasma aliquots. Chromatographic separation of drugs and Internal Standard (quinoxaline) was achieved with a gradient (acetonitrile and water + formic acid 0.05%) on a C18 reverse phase analytical column with 20 min of analytical run, at flow rate of 1 ml/min. Mean intra-day and inter-day precision for all compounds were 4.3 and 11.4%; mean accuracy was 1.5%; extraction recovery ranged within 95 and 114%. Calibration curves ranged from 10,000 to 62.5 ng/ml. The limit of quantification was set at 78.1 ng/ml for imatinib and at 62.5 ng/ml for dasatinib and nilotinib. This novel developed methodology allows a specific, sensitive and reliable simultaneous determination of the three tyrosine kinase inhibitors imatinib, dasatinib and nilotinib in a single chromatographic run, useful for drugs estimation in plasma of patients affected by chronic myeloid leukemia. 相似文献
992.
Jon Bielby Stefano Bovero Giuseppe Sotgiu Giulia Tessa Marco Favelli Claudio Angelini Stefano Doglio Frances C. Clare Enrico Gazzaniga Federica Lapietra Trenton W. J. Garner 《EcoHealth》2009,6(1):27-32
Batrachochytrium dendrobatidis (Bd), the causative agent of the amphibian disease chytridiomycosis, is an important factor in the global decline of amphibians. Within Europe, animals that exhibit clinical signs of the disease have only been reported in Spain despite the pathogen’s wide, but patchy, distribution on the continent. Recently, another occurrence of chytridiomycosis was reported in Euproctus platycephalus, the Sardinian brook newt, on the Mediterranean island of Sardinia, but without any evidence of fatal disease. We report further evidence of the emergence of Bd on Sardinia and the first evidence of lethal chytridiomycosis outside of Spain. Unusual mortalities of the Tyrrhenian painted frog (Discoglossus sardus) were found at three sites in the Limbara mountains of northern Sardinia. Molecular and histological screens of corpses, frogs, and tadpoles from these sites revealed infection with Bd. Infection and mortality occurred at locations that are unusual in terms of the published habitat requirements of the pathogen. Given the endemicity, the IUCN Red List status of the amphibian species on Sardinia, and the occurrence of infection and mortality caused by chytridiomycosis, there is serious reason for concern for the impact that disease emergence may have on the conservation of the amphibians of the island. 相似文献
993.
Based on traditional techniques and confocal laser scanning microscopy for external morphology, and immunohistochemistry for the muscular system, we describe here the segmental features of the antennal exopod of Artemia nauplii. Two kinds of serial elements are present, i.e. setae (with cuticular folds at their base) and ringlets (serially arranged sclerites separated by joint-like cuticular folds not extending to form complete rings around the appendage). The two series are usually not in register. The cuticular folds of the setae and of the ringlets are also sites of intermediate insertions of the three exopod muscles: as the two tegumentary structures are discordant in periodicity, this is also mirrored in the pattern of muscle insertions on the two sides of the appendage. Similar cases of segmental mismatch are known for the trunk of several arthropods, but segmental mismatch along the appendages has received very little attention. The occurrence of segmental mismatch in the naupliar appendages of both extant and fossil crustaceans is reviewed and it is suggested here to be a primitive feature of the exopods of both second antennae and mandibles. Problems in the interpretation of morphological evidence are discussed, also in relation to development and evolution of segmentation of naupliar appendages. 相似文献
994.
Anna U?arowska Giuseppe Dionisio Barbara Sarholz Hans-Peter Piepho Mingliang Xu Christina R?nn Ingvardsen Gerhard Wenzel Thomas Lübberstedt 《BMC plant biology》2009,9(1):15
Background
The potyviruses sugarcane mosaic virus (SCMV) and maize dwarf mosaic virus (MDMV) are major pathogens of maize worldwide. Two loci, Scmv1 and Scmv2, have ealier been shown to confer complete resistance to SCMV. Custom-made microarrays containing previously identified SCMV resistance candidate genes and resistance gene analogs were utilised to investigate and validate gene expression and expression patterns of isogenic lines under pathogen infection in order to obtain information about the molecular mechanisms involved in maize-potyvirus interactions. 相似文献995.
Johannes Voortman Thang V Pham Jaco C Knol Giuseppe Giaccone Connie R Jimenez 《Proteome science》2009,7(1):34-14
Background
Only a minority of patients with advanced non-small cell lung cancer (NSCLC) benefit from chemotherapy. Serum peptide profiling of NSCLC patients was performed to investigate patterns associated with treatment outcome. 相似文献996.
Oliaro-Bosso S Taramino S Viola F Tagliapietra S Ermondi G Cravotto G Balliano G 《Journal of enzyme inhibition and medicinal chemistry》2009,24(2):589-598
Human and murine lanosterol synthases (EC 5.4.99.7) were studied as targets of a series of umbelliferone aminoalkyl derivatives previously tested as inhibitors of oxidosqualene cyclases from other eukaryotes. Tests were carried out on cell cultures of human keratinocytes and mouse 3T3 fibroblasts incubated with radiolabeled acetate, and on homogenates prepared from yeast cells expressing human lanosterol synthase, incubated with radiolabeled oxidosqualene. In cell cultures of both human keratinocytes and mouse 3T3 fibroblasts, the observed inhibition of cholesterol biosynthesis was selective for oxidosqualene cyclase. The most active compounds bear an allylmethylamino chain in position-7 of the coumarin ring. The inhibition was critically dependent on the position and length of the inhibitor side chain, as well as on the type of aminoalkyl group inserted at the end of the same chain. Molecular docking analyses, carried out to clarify details of inhibitors/enzyme interactions, proved useful to explain the observed differences in inhibitory activities. 相似文献
997.
Marco Camanni Luca Bonino Elena Maria Delpiano Paola Berchialla Giuseppe Migliaretti Alberto Revelli Francesco Deltetto 《Reproductive biology and endocrinology : RB&E》2009,7(1):109-7
Background
this study aims to evaluate the effectiveness and safety of laparoscopic conservative management of ureteral endometriosis. 相似文献998.
999.
1000.
NA-Seq: A Discovery Tool for the Analysis of Chromatin Structure and Dynamics during Differentiation
Gaetano Gargiulo Samuel Levy Gabriele Bucci Mauro Romanenghi Lorenzo Fornasari Karen Y. Beeson Susanne M. Goldberg Matteo Cesaroni Marco Ballarini Fabio Santoro Natalie Bezman Gianmaria Frigè Philip D. Gregory Michael C. Holmes Robert L. Strausberg Pier Giuseppe Pelicci Fyodor D. Urnov Saverio Minucci 《Developmental cell》2009,16(3):466-481