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41.
Production and characterization of antibodies against HT-2 toxin and T-2 tetraol tetraacetate. 总被引:1,自引:1,他引:0 下载免费PDF全文
Three new immunogens which were prepared by conjugation of the carboxymethyl oxime (CMO) derivatives of HT-2 toxin, T-2 tetraol (T-2 4ol), and T-2 tetraol tetraacetate (T-2 4Ac) to bovine serum albumin (BSA) were tested for the production of antibodies against the major metabolites of T-2 toxin. Antibodies against HT-2 toxin and T-2 4Ac were obtained from rabbits 5 to 10 weeks after immunizing the animals with CMO-HT-2-BSA and CMO-T-2 4Ac-BSA conjugates. Immunization with CMO-T-2 4ol-BSA resulted in no antibody against T-2 4ol. The antibody produced against HT-2 toxin had great affinity for HT-2 toxin as well as good cross-reactivity with T-2 toxin. The relative cross-reactivities of anti-HT-2 toxin antibody with HT-2 toxin, T-2 toxin, iso-T-2 toxin, acetyl-T-2 toxin, 3'-OH HT-2, 3'-OH T-2, T-2 triol, and 3'-OH acetyl-T-2, were 100, 25, 10, 3.3, 0.25, 0.15, 0.12 and 0.08%, respectively. Antibody against CMO-T-2 4Ac was very specific for T-2 4Ac and had less than 0.1% cross-reactivity with T-2 toxin, HT-2 toxin, acetyl-T-2 toxin, diacetoxyscirpenol, deoxynivalenol, and deoxynivalenol triacetate as compared with T-2 4Ac. The detection limits for HT-2 toxin and T-2 4ol by radioimmunoassay were approximately 0.1 and 0.5 ng per assay, respectively. 相似文献
42.
An electrophoretic karyotype for Schizosaccharomyces pombe by pulsed field gel electrophoresis. 总被引:44,自引:25,他引:19 下载免费PDF全文
C L Smith T Matsumoto O Niwa S Klco J B Fan M Yanagida C R Cantor 《Nucleic acids research》1987,15(11):4481-4489
The three chromosomal DNAs of S. pombe have been fractionated by pulsed field gel electrophoresis. The resulting molecular karyotype will greatly speed gene mapping in this organism, and it indicates that the separation range of the technique extends to DNA molecules as large as 9,000,000 base pairs. 相似文献
43.
记辽宁东部新鳞齿鱼属一新种 总被引:1,自引:0,他引:1
本文记述了产自辽宁东部红庙子盆地下桦皮甸子组的新鳞齿鱼属—新种——Neolepidotes liaodongensis sp. nov..根据新材料,将 Neolepidotes 与 Lepidotes 等属作了补充比较,增订了新鳞齿鱼属的特征. 相似文献
44.
45.
光质对一品红叶离体培养中形态发生及生化变化的影响 总被引:3,自引:0,他引:3
本文讨论了将光质应用于一品红(Euphorbia pulcherrima)大规模细胞繁殖及形态发生调控的可行性,并测定了一些形态及生化指标作为这种调控的参数。 以一品红试管苗的叶片作为外植体,置于MS培养基中并分别培养于白(W)、红(R)、黄(Y)、蓝(B)、绿(G)色光及黑暗(D)下,一个月后进行测定。蓝光和黄光明显促进出苗。而愈伤组织形成的情况则正相反,红光利于愈伤组织的形成,黄光效果最差。过氧化物酶、过氧化氢酶活力及蛋白质、RNA、碳水化合物的含量与愈伤组织形成有较为一致的关系,但与分化不直接相关。 实验中还测定了叶绿素a、叶绿素b及类胡萝卜素的含量。白光与蓝光均能促进叶绿素及类胡萝卜素的合成,但蓝光中chl b/chl a的比率较高。此外,本文尚涉及暗处理中的晶质化现象(vitrification)及其成因。 相似文献
46.
The kinematics of an area-conserving tank-treading disk-shaped red blood cell membrane is studied using the stream function
method suggested by Secomb and Skalak (Q. Jl Mech. appl. Math.
35, Pt 2, 233–247, 1982). Two simple area-conserving velocity fields are superimposed to satisfy the continuity condition at
the curved edges of the disk. A differential equation for the trajectory of any material point of the membrane is derived.
The requirement of synchrony of the cycle for all membrane points leads to an integral equation which determines a magnitude
function. An approximate solution is made possible by assuming small trajectory deflections. 相似文献
47.
Level of protein kinase C activity correlates directly with resistance to adriamycin in murine fibrosarcoma cells 总被引:3,自引:0,他引:3
In this report, we demonstrate a direct correlation between protein kinase C (PKC) activity and adriamycin (ADR) resistance in mouse fibrosarcoma cells. PKC activity was measured in four murine UV-2237M fibrosarcoma cell lines that differed in the degrees to which they expressed resistance to ADR, which is an inhibitor of PKC. A comparison of the four cell lines revealed a positive correlation between the level of PKC activity and resistance to ADR. Incubation of the cells with the PKC inhibitor H-7 produced a partial reversal of ADR resistance. Taken together, these results suggest a role for PKC in the mechanism of ADR resistance. 相似文献
48.
Exon-intron organization, expression, and chromosomal localization of the human motilin gene 总被引:1,自引:0,他引:1
H Yano Y Seino J Fujita Y Yamada N Inagaki J Takeda G I Bell R L Eddy Y S Fan M G Byers 《FEBS letters》1989,249(2):248-252
The human motilin gene has been isolated and characterized. The gene spans about 9 kilobase pairs (kb) and the 0.7 kb motilin mRNA is encoded by five exons. The 22-amino-acid motilin sequence is encoded by exons 2 and 3. The human motilin gene was mapped to the p21.2----p21.3 region of chromosome 6 by hybridization of the cloned cDNA to DNAs from a panel of reduced human-mouse somatic cell hybrids and by in situ hybridization to human prometaphase chromosomes. RNA blotting using RNA prepared from various regions of the human gastrointestinal tract revealed high levels of motilin mRNA in duodenum and lower levels in the antrum of the stomach; motilin mRNA could not be detected by this procedure in the esophagus, cardia of the stomach, descending colon or gallbladder. 相似文献
49.
Yuzuru Mikami Sen Feng Yu Katsukiyo Yazawa Kazutaka Fukushima Akio Maeda Jun Uno Kiyoshi Terao Naoki Saito Akinori Kubo Ken -ichiro Suzuki 《Mycopathologia》1990,112(2):113-118
During our studies on toxic substances from clinically isolated Nocarida, a new isolate identified as Nocardia otitidiscaviarum from cutaneous nocardiosis was found to produce a toxic substance called HS-6 that had strong in vitro as well as in vivo toxicity. The mouse intraperitoneal LD50 value was 1.25 mg/kg and the ED50 value for L1210 cultured cells was 0.3 ng/ml. The structure of HS-6 was determined and found to belong to the 16-membered macrocyclic group with a molecular formula of C43H68O12. HS-6 also showed activity against pathogenic fungi such as Cryptococcus neoformans. 相似文献
50.