全文获取类型
收费全文 | 2126篇 |
免费 | 66篇 |
国内免费 | 1篇 |
出版年
2022年 | 13篇 |
2021年 | 37篇 |
2020年 | 18篇 |
2019年 | 29篇 |
2018年 | 48篇 |
2017年 | 31篇 |
2016年 | 42篇 |
2015年 | 41篇 |
2014年 | 71篇 |
2013年 | 103篇 |
2012年 | 115篇 |
2011年 | 146篇 |
2010年 | 111篇 |
2009年 | 75篇 |
2008年 | 77篇 |
2007年 | 88篇 |
2006年 | 80篇 |
2005年 | 70篇 |
2004年 | 66篇 |
2003年 | 66篇 |
2002年 | 86篇 |
2001年 | 95篇 |
2000年 | 66篇 |
1999年 | 38篇 |
1998年 | 22篇 |
1994年 | 10篇 |
1992年 | 27篇 |
1991年 | 32篇 |
1990年 | 29篇 |
1989年 | 29篇 |
1988年 | 29篇 |
1987年 | 31篇 |
1986年 | 28篇 |
1985年 | 30篇 |
1984年 | 28篇 |
1983年 | 23篇 |
1979年 | 15篇 |
1978年 | 9篇 |
1977年 | 10篇 |
1976年 | 11篇 |
1975年 | 22篇 |
1974年 | 22篇 |
1973年 | 21篇 |
1972年 | 18篇 |
1971年 | 17篇 |
1970年 | 14篇 |
1968年 | 18篇 |
1967年 | 13篇 |
1966年 | 11篇 |
1965年 | 9篇 |
排序方式: 共有2193条查询结果,搜索用时 15 毫秒
61.
Maren de Vries Adil S. Mohamed Rachel A. Prescott Ana M. Valero-Jimenez Ludovic Desvignes Rebecca OConnor Claire Steppan Joseph C. Devlin Ellie Ivanova Alberto Herrera Austin Schinlever Paige Loose Kelly Ruggles Sergei B. Koralov Annaliesa S. Anderson Joseph Binder Meike Dittmann 《Journal of virology》2021,95(10)
62.
63.
Natalie V. Klinard Jordan K. Matley Silviya V. Ivanova Sarah M. Larocque Aaron T. Fisk Timothy B. Johnson 《Journal of fish biology》2021,98(1):237-250
Understanding predator–prey interactions and food web dynamics is important for ecosystem-based management in aquatic environments, as they experience increasing rates of human-induced changes, such as the addition and removal of fishes. To quantify the post-stocking survival and predation of a prey fish in Lake Ontario, 48 bloater Coregonus hoyi were tagged with acoustic telemetry predation tags and were tracked on an array of 105 acoustic receivers from November 2018 to June 2019. Putative predators of tagged bloater were identified by comparing movement patterns of six species of salmonids (i.e., predators) in Lake Ontario with the post-predated movements of bloater (i.e., prey) using a random forests algorithm, a type of supervised machine learning. A total of 25 bloater (53% of all detected) were consumed by predators on average (± S.D. ) 3.1 ± 2.1 days after release. Post-predation detections of predators occurred for an average (± S.D. ) of 78.9 ± 76.9 days, providing sufficient detection data to classify movement patterns. Tagged lake trout Salvelinus namaycush provided the most reliable classification from behavioural predictor variables (89% success rate) and was identified as the main consumer of bloater (consumed 50%). Movement networks between predicted and tagged lake trout were significantly correlated over a 6 month period, supporting the classification of lake trout as a common bloater predator. This study demonstrated the ability of supervised learning techniques to provide greater insight into the fate of stocked fishes and predator–prey dynamics, and this technique is widely applicable to inform future stocking and other management efforts. 相似文献
64.
Thomsen’s (TM) and Becker’s (BM) myotonias are nondystrophic myotonias. At present, 150 mutations in the CLCN1 gene, which results in the development of TM and BM, have been described. The c.2680C>T (p.Arg894*) is the most common mutation. In the Northern Scandinavian countries, the population frequency of this mutation is 0.87%, while in the Russian Federation, it is equal to 1.2% (this study). Based on the results of a molecular-genetic analysis of CLCN1 gene in patients with nondystrophic myotonias, the calculated frequency of TM and BM in Russia is 1: 8165 and 1: 710, respectively. We have conducted haplotype analysis using microsatellite markers and intragene SNP, which has shown that the prevalence of p.Arg894* mutation in Russia results from the founder effect, and the time of its scattering is 3680 ± 1240 years. 相似文献
65.
66.
67.
Wayne Reeve Rui Tian Sofie De Meyer Vanessa Melino Jason Terpolilli Julie Ardley Ravi Tiwari John Howieson Ronald Yates Graham O’Hara Mohamed Ninawi Hazuki Teshima David Bruce Chris Detter Roxanne Tapia Cliff Han Chia-Lin Wei Marcel Huntemann James Han I-Min Chen Konstantinos Mavromatis Victor Markowitz Natalia Ivanova Galina Ovchinnikova Ioanna Pagani Amrita Pati Lynne Goodwin Sam Pitluck Tanja Woyke Nikos Kyrpides 《Standards in genomic sciences》2013,9(2):243-253
Rhizobium leguminosarum bv. trifolii strain TA1 is an aerobic, motile, Gram-negative, non-spore-forming rod that is an effective nitrogen fixing microsymbiont on the perennial clovers originating from Europe and the Mediterranean basin. TA1 however is ineffective with many annual and perennial clovers originating from Africa and America. Here we describe the features of R. leguminosarum bv. trifolii strain TA1, together with genome sequence information and annotation. The 8,618,824 bp high-quality-draft genome is arranged in a 6 scaffold of 32 contigs, contains 8,493 protein-coding genes and 83 RNA-only encoding genes, and is one of 20 rhizobial genomes sequenced as part of the DOE Joint Genome Institute 2010 Community Sequencing Program. 相似文献
68.
Jason Terpolilli Yvette Hill Rui Tian John Howieson Lambert Br?u Lynne Goodwin James Han Konstantinos Liolios Marcel Huntemann Amrita Pati Tanja Woyke Konstantinos Mavromatis Victor Markowitz Natalia Ivanova Nikos Kyrpides Wayne Reeve 《Standards in genomic sciences》2013,9(2):315-324
Ensifer meliloti WSM1022 is an aerobic, motile, Gram-negative, non-spore-forming rod that can exist as a soil saprophyte or as a legume microsymbiont of Medicago. WSM1022 was isolated in 1987 from a nodule recovered from the roots of the annual Medicago orbicularis growing on the Cyclades Island of Naxos in Greece. WSM1022 is highly effective at fixing nitrogen with M. truncatula and other annual species such as M. tornata and M. littoralis and is also highly effective with the perennial M. sativa (alfalfa or lucerne). In common with other characterized E. meliloti strains, WSM1022 will nodulate but fixes poorly with M. polymorpha and M. sphaerocarpos and does not nodulate M. murex. Here we describe the features of E. meliloti WSM1022, together with genome sequence information and its annotation. The 6,649,661 bp high-quality-draft genome is arranged into 121 scaffolds of 125 contigs containing 6,323 protein-coding genes and 75 RNA-only encoding genes, and is one of 100 rhizobial genomes sequenced as part of the DOE Joint Genome Institute 2010 Genomic Encyclopedia for Bacteria and Archaea-Root Nodule Bacteria (GEBA-RNB) project. 相似文献
69.
Thomas J. Jaworek Elodie M. Richard Anna A. Ivanova Arnaud P. J. Giese Daniel I. Choo Shaheen N. Khan Sheikh Riazuddin Richard A. Kahn Saima Riazuddin 《PLoS genetics》2013,9(9)
Exome sequencing coupled with homozygosity mapping was used to identify a transition mutation (c.794T>C; p.Leu265Ser) in ELMOD3 at the DFNB88 locus that is associated with nonsyndromic deafness in a large Pakistani family, PKDF468. The affected individuals of this family exhibited pre-lingual, severe-to-profound degrees of mixed hearing loss. ELMOD3 belongs to the engulfment and cell motility (ELMO) family, which consists of six paralogs in mammals. Several members of the ELMO family have been shown to regulate a subset of GTPases within the Ras superfamily. However, ELMOD3 is a largely uncharacterized protein that has no previously known biochemical activities. We found that in rodents, within the sensory epithelia of the inner ear, ELMOD3 appears most pronounced in the stereocilia of cochlear hair cells. Fluorescently tagged ELMOD3 co-localized with the actin cytoskeleton in MDCK cells and actin-based microvilli of LLC-PK1-CL4 epithelial cells. The p.Leu265Ser mutation in the ELMO domain impaired each of these activities. Super-resolution imaging revealed instances of close association of ELMOD3 with actin at the plasma membrane of MDCK cells. Furthermore, recombinant human GST-ELMOD3 exhibited GTPase activating protein (GAP) activity against the Arl2 GTPase, which was completely abolished by the p.Leu265Ser mutation. Collectively, our data provide the first insights into the expression and biochemical properties of ELMOD3 and highlight its functional links to sound perception and actin cytoskeleton. 相似文献
70.
Kuznetsova A. I. Ivanova E. A. Samylina O. S. Kurbanova F. G. Gruzdev D. S. Kanapatskiy T. A. Pimenov N. V. 《Microbiology》2020,89(6):670-684
Microbiology - Analysis of the taxonomic structure of prokaryotic microbial complexes of the saline soils in the Lake Elton area and their comparative characteristics were carried out for a soil... 相似文献