全文获取类型
收费全文 | 106215篇 |
免费 | 3833篇 |
国内免费 | 1823篇 |
出版年
2022年 | 550篇 |
2021年 | 1269篇 |
2020年 | 935篇 |
2019年 | 1053篇 |
2018年 | 1908篇 |
2017年 | 1796篇 |
2016年 | 3832篇 |
2015年 | 7563篇 |
2014年 | 7403篇 |
2013年 | 7292篇 |
2012年 | 6820篇 |
2011年 | 3764篇 |
2010年 | 3148篇 |
2009年 | 2968篇 |
2008年 | 1658篇 |
2007年 | 1473篇 |
2006年 | 1378篇 |
2005年 | 7348篇 |
2004年 | 5931篇 |
2003年 | 4083篇 |
2002年 | 1579篇 |
2001年 | 1546篇 |
2000年 | 721篇 |
1999年 | 1854篇 |
1998年 | 570篇 |
1992年 | 2122篇 |
1991年 | 2151篇 |
1990年 | 2198篇 |
1989年 | 2105篇 |
1988年 | 2023篇 |
1987年 | 1904篇 |
1986年 | 1678篇 |
1985年 | 1707篇 |
1984年 | 1128篇 |
1983年 | 862篇 |
1982年 | 487篇 |
1979年 | 1079篇 |
1978年 | 759篇 |
1977年 | 606篇 |
1976年 | 629篇 |
1975年 | 872篇 |
1974年 | 994篇 |
1973年 | 1005篇 |
1972年 | 953篇 |
1971年 | 928篇 |
1970年 | 820篇 |
1969年 | 830篇 |
1968年 | 734篇 |
1967年 | 751篇 |
1966年 | 584篇 |
排序方式: 共有10000条查询结果,搜索用时 31 毫秒
81.
P T M?nnist? 《Medical biology》1980,58(6):310-318
The subcellular distribution of the TRH-like immunoreactivity in the rat hypothalamus and brain was studied. In differential centrifugation, the 900 g for 10 min supernatant (S1) of the hypothalamus or brain contained 61--79% of the total TRH. At 11,000 g for 20 min, 51--73% of the TRH in S1 was sedimented. When the hypothalamic S1 was fractioned under non-equilibrium conditions at 25 degrees C, two populations of TRH-containing particles were observed in several types of continuous linear density gradients. Metrizamide and sucrose gradients affected TRH-assay. TRH-particles were very light in Percol-gradients. Isotonic dextran 40,000-sucrose gradients gave the most reproducible results. In these gradients, the large TRH-particles (35%) equilibrated at 1.055--1.060 kg/l and the small ones (23%) at 1.041--1.047 kg/l. Working at 4 degrees C decreased the amount of large TRH-particles. The apparently larger particles contained cytoplasmic and mitochondrial enzymes and were sensitive to hypoosmotic shock like synaptosomes. Electron micrographs confirmed that these particles were synaptosomes. The true nature of the small particles remained unclear but morphologically a part of them were also synaptosomes. Treatment of the animals with reserpine (10 mg/kg i.p., 24 h), with 6-hydroxydopamine (100 microgram/rat i.c.v.) or with 5,7-dihydroxytryptamine (200 microgram/rat i.c.v.) did not affect significantly TRH-recovery or distribution in the hypothalamus. 相似文献
82.
The present study has shown that on the level of the parasitic system the epidemic process is a biological system, wherein the host population serves as the internal regulator, the mechanism of transmission serves as the external regulator and the parasite population, as the regulated object. The biological regulating mechanisms of the epidemic process have fundamental differences in the groups of infectious with various mechanisms of transmission, and the specific nature of the mechanism of transmission determines the peculiar features of the biological mechanism which governs the self-regulation of the epidemic process. In contrast, on a higher level of the organization of the epidemic process, i. e. on the level of the socio-ecological system, the epidemic process is a biosocial system, wherein the human society serves as the regulator, the parasitic system serves as the regulated object and the mechanism of transmission plays the role of the filter which determines the scope of social factors, most important in the regulation of the epidemic process in a given infection. The spontaneous regulation of the epidemic process is the freed forward channel from the regulator to the regulated object, and the controlled regulation is the feedback channel. 相似文献
83.
84.
为了解内蒙古地区蜱虫病毒组学的本底数据,采用病毒宏基因组学方法对在内蒙古阿拉善盟左旗、右旗和四子王旗地区3个采样点采集骆驼和羊体表寄生的1789只蜱虫样品进行病毒宏基因组学分析,并对特定病毒进行巢式PCR扩增和测序,通过Clustal W和MEGA7.0等生物信息学软件对获得的病毒基因序列进行遗传进化分析.数据显示,蜱虫样品携带包括植物、脊椎动物和非脊椎动物等来源的17个病毒科和一些未分类的病毒;其中,2株弹状病毒具有丰富的遗传多样性,与新疆地区和长江地区的弹状病毒的同源性达到98.5%和96.26%,提示蜱虫弹状病毒可能是通过羊和骆驼等动物贸易导致了新疆和内蒙古地区,以及内地的跨区域传播;细小病毒仅在羊来源的蜱虫中检测到,与中国河北地区的山羊血清中的细小病毒形成同一进化分支,我们推测蜱虫细小病毒在国内不同地区间可跨区域传播,在进化分析过程中,发现这种病毒与多种的细小病毒的同源性都不低于50%,提示细小病毒可能具有遗传稳定性;Tamdy病毒与来自阿塞拜疆、乌兹别克斯坦和美国的Tamdy病毒均具有极高的同源性,结果显示该病毒在内蒙古地区已经出现,并存在潜在流行的可能,有必要对Tamdy病毒进行进一步的监测;在本研究中,我们鉴定的白蛉病毒与来自新疆的亚洲璃眼蜱所携带的博乐蜱虱病毒形成同一个进化分支,与新型布尼亚病毒和Heartland virus病毒的同源性达到50%以上,该结果提示,我们发现的蜱虫白蛉病毒可能具有潜在的致病性,需要对其流行情况和致病性进行监测和研究.本研究为完善内蒙古部分地区蜱虫病毒的多样性和本底情况提供了重要的基础数据. 相似文献
85.
Protoplasts of Schizosaccharomyces pombe, grown on a rich nutrient medium, were treated with a peptide factor isolated from cultures of the protozoan Tetrahymena pyriformis. The peptide factor is known to inhibit RNA synthesis in Tetrahymena. It has now been shown that the peptide factor also inhibits RNA synthesis in yeast protoplasts without affecting protein synthesis. 相似文献
86.
87.
88.
89.
90.