排序方式: 共有78条查询结果,搜索用时 296 毫秒
31.
Diwakar Aggarwal Neha Jaiswal Anil Kumar M. Sudhakara Reddy 《Journal of plant biochemistry and biotechnology.》2013,22(4):382-391
Efficient Agrobacterium tumefaciens mediated T-DNA delivery and subsequent shoot organogenesis has been achieved from Bacopa monnieri. Various factors influenced T-DNA delivery as evident from transient GUS assay. The transient GUS expression was significantly higher (97.7 %) in explants that were pre-cultured before bacterial infection on medium supplemented with 100 μM acetosyringone. Incorporation of acetosyringone into the co-cultivation medium also enhanced transient GUS activity. Explant injury with carborundum paper, co-cultivation period of 2 days and a bacterial density of 0.4 OD600 showed higher transient GUS expression. Following co-cultivation, shoot organogenesis was achieved from leaf segments on basal Murashige and Skoog medium containing 58 mM sucrose. Supplementation of antibiotics (cefotaxime or carbenicillin) at > 250 μg/ml into the medium significantly promoted shoot organogenesis from leaf explants (71.5 % in control and > 83.0 % on medium containing 500 μg/ml of carbenicillin or cefotaxime). Stable transformation of regenerated shoots was confirmed on the basis of GUS activity and PCR amplification of DNA fragments specific to reporter gene (uidA) and selection marker gene (nptII). The expression level of nptII gene in independent transgenic lines was studied using quantitative real time-PCR. Stable transformed shoots after rooting were successfully established in the pots. 相似文献
32.
Keerthana Krishnan Anita L. Steptoe Hilary C. Martin Diwakar R. Pattabiraman Katia Nones Nic Waddell Mythily Mariasegaram Peter T. Simpson Sunil R. Lakhani Alexander Vlassov Sean M. Grimmond Nicole Cloonan 《RNA (New York, N.Y.)》2013,19(12):1767-1780
Metastasis is a complex, multistep process involved in the progression of cancer from a localized primary tissue to distant sites, often characteristic of the more aggressive forms of this disease. Despite being studied in great detail in recent years, the mechanisms that govern this process remain poorly understood. In this study, we identify a novel role for miR-139-5p in the inhibition of breast cancer progression. We highlight its clinical relevance by reviewing miR-139-5p expression across a wide variety of breast cancer subtypes using in-house generated and online data sets to show that it is most frequently lost in invasive tumors. A biotin pull-down approach was then used to identify the mRNA targets of miR-139-5p in the breast cancer cell line MCF7. Functional enrichment analysis of the pulled-down targets showed significant enrichment of genes in pathways previously implicated in breast cancer metastasis (P < 0.05). Further bioinformatic analysis revealed a predicted disruption to the TGFβ, Wnt, Rho, and MAPK/PI3K signaling cascades, implying a potential role for miR-139-5p in regulating the ability of cells to invade and migrate. To corroborate this finding, using the MDA-MB-231 breast cancer cell line, we show that overexpression of miR-139-5p results in suppression of these cellular phenotypes. Furthermore, we validate the interaction between miR-139-5p and predicted targets involved in these pathways. Collectively, these results suggest a significant functional role for miR-139-5p in breast cancer cell motility and invasion and its potential to be used as a prognostic marker for the aggressive forms of breast cancer. 相似文献
33.
The aim of this work was to investigate the optimal process conditions leading to high glucose yield (over 80 %) after wet explosion (WEx) pretreatment and enzymatic hydrolysis. The study focused on determining the “sweet spot” where the glucose yield obtained is optimized compared to the cost of the enzymes. WEx pretreatment was conducted at different temperatures, times, and oxygen concentrations to determine the best WEx pretreatment conditions for the most efficient enzymatic hydrolysis. Enzymatic hydrolysis was further optimized at the optimal conditions using central composite design of response surface methodology with respect to two variables: Cellic® CTec2 loading [5 to 40 mg enzyme protein (EP)/g glucan] and substrate concentration (SC) (5 to 20 %) at 50 °C for 72 h. The most efficient and economic conditions for corn stover conversion to glucose were obtained when wet-exploded at 170 °C for 20 min with 5.5 bar oxygen followed by enzymatic hydrolysis at 20 % SC and 15 mg EP/g glucan (5 filter paper units) resulting in a glucose yield of 84 %. 相似文献
34.
The phenomenon of hybrid vigor (heterosis) has long been harnessed by plant breeders to improve world food production. However, the changes that are essential for heterotic responses and the mechanisms responsible for heterosis remain undefined. Large increases in biomass and yield in high-heterosis hybrids suggest that alterations in bioenergetic processes may contribute to heterosis. Progeny from crosses between various inbred lines vary in the extent of vigor observed. Field-grown maize F(1) hybrids that consistently exhibited either low or high heterosis across a variety of environments were examined for changes in proteins that may be correlated with increased plant vigor and yield. Unpollinated ears at the time of flowering (ear shoots) were selected for the studies because they are metabolically active, rich in mitochondria, and the sizes of the ears are diagnostic of yield heterosis. Total protein and mitochondrial proteomes were compared among low- and higher-heterosis hybrids. Two-dimensional difference gel electrophoresis was used to identify allelic and/or isoform differences linked to heterosis. Identification of differentially regulated spots by mass spectrometry revealed proteins involved in stress responses as well as primary carbon and protein metabolism. Many of these proteins were identified in multiple spots, but analysis of their abundances by label-free mass spectrometry suggested that most of the expression differences were due to isoform variation rather than overall protein amount. Thus, our proteomics studies suggest that expression of specific alleles and/or post-translational modification of specific proteins correlate with higher levels of heterosis. 相似文献
35.
Diwakar Dahal Dimitri Heintz Alain Van Dorsselaer Hans-Peter Braun Kerstin Wydra 《Plant Physiology and Biochemistry》2009,47(9):838-846
A comparative proteome analysis was initiated to systematically investigate the physiological response of tomato (Solanum lycopersicum) to infection with Ralstonia solanacearum, causal agent of bacterial wilt. Plants of the susceptible tomato recombinant inbred line NHG3 and the resistant NHG13 were either infected or not infected with R. solanacearum and subsequently used for proteome analysis. Two-dimensional isoelectric focussing/sodium dodecyl-sulphate polyacrylamide gel electrophoresis (2-D IEF/SDS-PAGE) allowed the separation of about 650–690 protein spots per analysis. Twelve proteins were of differential abundance in susceptible plants in response to bacterial infection, while no differences were observed in the resistant genotype. LC-MS/MS analysis of these spots revealed 12 proteins, six of which were annotated as plant and six as bacterial proteins. Among the plant proteins, two represent pathogenesis related (PR) proteins, one stress response protein, one enzyme of carbohydrate and energy metabolism, and one hypothetical protein. A constitutive difference between resistant and susceptible lines was not found. 相似文献
36.
Machado E Kaczmarski M Braida B Ordejón P Garg D Norman J Cheng H 《Journal of molecular modeling》2007,13(6-7):861-864
Processes for the deposition of copper films on transition metal barrier layers by means CVD using organometallic precursors
are often found to lead to poor adhesion characteristics of the grown film. By means of first-principles molecular dynamics
simulations, we show that the source of the problem is the strong reactivity of the surfaces toward the precursors, which
decompose spontaneously upon contact with the surface leading to contamination of the interface. Our simulations consider
Ti, Ta, and W as barrier layers, and Cu(hfac)-(tmvs) as precursor. In contrast, we show that surfaces of these metals properly
passivated with nitrogen, in such a way that only N atoms are exposed on the surface, are much less active and do not lead
to decomposition of the precursor. We propose this passivation procedure as a practical solution to the adhesion problem.
Figure CupraSelect on the WN (100) surface 相似文献
37.
Dubey A Diwakar SK Rawat SK Kumar P Batra N Joshi A Singh J 《Preparative biochemistry & biotechnology》2007,37(1):47-58
Ionically bound peroxidases (POD) were salt extracted from the pulp of four Indian apple varieties, i.e., Golden delicious HP, Golden delicious JK, Red delicious, and Royal delicious. They were precipitated with chilled ethanol. Thermal treatments of partially purified enzymes were given from 40-70 degrees C for 30 minutes. Golden delicious HP peroxidase showed thermostability at 60 degrees C, while three other peroxidases were observed at 50 degrees C. Phenolic compounds (i.e., caffeic acid, ferulic acids, p-coumaric acid, protocatechuic acid) and metal ions (i.e., Cu2+ and Fe2+) activated all apple peroxidases. However, Mn2+ inhibited the peroxidases from Golden delicious HP, Golden delicious JK, and Red delicious, and a substantial increase was observed in Royal delicious peroxidase. Mg2+ inhibited the peroxidases from Golden delicious HP and Red delicious, but marginal activation was reported in peroxidases from Golden delicious JK and Royal delicious. Zn2+ established stimulation in Golden delicious HP and Golden delicious JK peroxidases, but inhibition was observed in peroxidases in Red delicious and Royal delicious.. Methionine, proline, tryptophan, and valine stimulated all four apple peroxidases, but cysteine showed inhibition in Golden delicious JK. 相似文献
38.
Bhuwan Prasad Awasthi Prakash Chaudhary Diwakar Guragain Jun-Goo Jee Jung-Ae Kim Byeong-Seon Jeong 《Journal of enzyme inhibition and medicinal chemistry》2021,36(1):1884
Sorafenib is recommended as the primary therapeutic drug for patients with hepatocellular carcinoma. To discover a new compound that avoids low response rates and toxic side effects that occur in sorafenib therapy, we designed and synthesized new hybrid compounds of sorafenib and 2,4,5-trimethylpyridin-3-ols. Compound 6 was selected as the best of 24 hybrids that inhibit each of the four Raf kinases. The anti-proliferative activity of 6 in HepG2, Hep3B, and Huh7 cell lines was slightly lower than that of sorafenib. However, in H6c7 and CCD841 normal epithelial cell lines, the cytotoxicity of 6 was much lower than that of sorafenib. In addition, similar to sorafenib, compound 6 inhibited spheroid forming ability of Hep3B cells in vitro and tumour growth in a xenograft tumour model of the chick chorioallantoic membrane implanted with Huh7 cells. Compound 6 may be a promising candidate targeting hepatocellular carcinoma with low toxic side effects on normal cells. 相似文献
39.
N Anand P Singh A Sharma S Tiwari V Singh DK Singh KK Srivastava BN Singh RP Tripathi 《Bioorganic & medicinal chemistry》2012,20(17):5150-5163
A synthetic strategy to access small libraries of triazolylmethoxy chalcones 4{1-20}, triazolylmethoxy flavanones 5{1-10} and triazolylmethoxy aminopyrimidines 6{1-17} from a common substrate 4-propargyloxy-2-hydroxy acetophenone using a set of different reactions has been developed. The chalcones and flavanones were screened against mycobacterial FAS-II pathway using a recombinant mycobacterial strain, against which the most potent compound showed ~88% inhibition in bacterial growth and substantially induction of reporter gene activity at 100μM concentration. The triazolylmethoxy aminopyrimdines were screened against PknG of Mycobaceterium tuberculosis displaying moderate to good activity (23-53% inhibition at 100μM), comparable to the action of a standard inhibitor. 相似文献
40.