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51.
52.
A Emami-Khoyi DA Hartley RH Cruickshank LJ Boren JG Ross 《New Zealand journal of zoology.》2016,43(4):322-335
New Zealand fur seals are one of many pinniped species that survived the commercial sealing of the eighteenth and nineteenth centuries in dangerously low numbers. After the enforcement of a series of protection measures in the early twentieth century, New Zealand fur seals began to recover from the brink of extinction. We examined the New Zealand fur seal populations of Banks Peninsula, South Island, New Zealand using the mitochondrial DNA control region. We identified a panmictic population structure around Banks Peninsula. The most abundant haplotype in the area showed a slight significant aggregated structure. The Horseshoe Bay colony showed the least number of shared haplotypes with other colonies, suggesting a different origin of re-colonisation of this specific colony. The effective population size of the New Zealand fur seal population at Banks Peninsula was estimated at approximately 2500 individuals. The exponential population growth rate parameter for the area was 35, which corresponds to an expanding population. In general, samples from adjacent colonies shared 4.4 haplotypes while samples collected from colonies separated by between five and eight bays shared 1.9 haplotypes. The genetic data support the spill-over dynamics of colony expansion already suggested for this species. Approximate Bayesian computations analysis suggests re-colonisation of the area from two main clades identified across New Zealand with a most likely admixture coefficient of 0.41 to form the Banks Peninsula population. Approximate Bayesian computations analysis estimated a founder population size of approximately 372 breeding individuals for the area, which then rapidly increased in size with successive waves of external recruitment. The population of fur seals in the area is probably in the late phase of maturity in the colony expansion dynamic. 相似文献
53.
Purification and characterization of Dolichos lablab lectin 总被引:1,自引:0,他引:1
The mannose/glucose-binding Dolichos lablab lectin (designated DLL) has
been purified from seeds of Dolichos lablab (hyacinth bean) to
electrophoretic homogeneity by affinity chromatography on an ovalbumin-
Sepharose 4B column. The purified lectin gave a single symmetric protein
peak with an apparent molecular mass of 67 kDa on gel filtration
chromatography, and five bands ranging from 10 kDa to 22 kDa upon SDS-PAGE.
N-Terminal sequence analysis of these bands revealed subunit heterogeneity
due to posttranslational proteolytic truncation at different sites mostly
at the carboxyl terminus. The carbohydrate binding properties of the
purified lectin were investigated by three different approaches:
hemagglutination inhibition assay, quantitative precipitation inhibition
assay, and ELISA. On the basis of these studies, it is concluded that the
Dolichos lablab lectin has neither an extended carbohydrate combining site,
nor a hydrophobic binding site adjacent to it. The carbohydrate combining
site of DLL appears to most effectively accommodate a nonreducing terminal
alpha-d-mannosyl unit, and to be complementary to the C-3, C-4, and C-6
equatorial hydroxyl groups of alpha-d-mannopyranosyl and
alpha-d-glucopyranosyl residues. DLL strongly precipitates murine IgM but
not IgG, and the recent finding that this lectin interacts specifically
with NIH 3T3 fibroblasts transfected with the Flt3 tyrosine kinase receptor
and preserves human cord blood stem cells and progenitors in a quiescent
state for prolonged periods in culture, make this lectin a valuable tool in
biomedical research.
相似文献
54.
55.
Following water quality and minimum flow improvements to the impounded Tennessee and Cumberland Rivers, juvenile lake sturgeon (Acipenser fulvescens) have been restocked annually since 2000. Our goal was to seasonally assess foraging mode of this recovering population in Ft. Loudoun Reservoir in the Upper Tennessee River. During 2014–15, individuals were captured using trot-lines in a 13-km reach that supports the greatest density of lake sturgeon. We used colonic flushing and gastric lavage techniques to obtain diet. We took systematic benthic sediment grabs along multiple transects throughout the reach and opportunistically deployed rock cages filled with hard substrates to assess potential prey that colonize hard surfaces. Foraging modes of lake sturgeon were determined by comparing relative abundances of invertebrate taxa in the gut contents (6581 invertebrates) of 28 fish to the relative abundances of the same invertebrate taxa collected from the resource base (1667 invertebrates). Proportional similarity, Levin’s niche breadth, and Manly’s index were used to assess the degree of prey selectivity. Lake sturgeon fed selectively on a narrow range of available prey consisting mostly of larval chironomids (93% composition by number during warm season, 96% during cool season), some genera of which they prey upon selectively, primarily Chironomus sp., but to a lesser extent Procladius, Ablabesmyia, Coelotanypus, and Cryptochironomus spp. Meanwhile, other abundant taxa in the resource base were avoided, such as Oligochaetes, Hexagenia mayflies, and the chironomid Glyptotendipes. Our results illustrate that assessing seasonally available prey from habitat utilized by lake sturgeon is important when investigating diet preference. 相似文献
56.
Pascale Simard Hugo Galarneau Sébastien Marois Daniel Rusu Caroline D Hoemann Patrice E Poubelle Hani El-Gabalawy Maria JG Fernandes 《Arthritis research & therapy》2009,11(3):R74-10
Introduction
Osteoarthritis is characterized by the progressive destruction of cartilage in the articular joints. Novel therapies that promote resurfacing of exposed bone in focal areas are of interest in osteoarthritis because they may delay the progression of this disabling disease in patients who develop focal lesions. Recently, the addition of 80% deacetylated chitosan to cartilage microfractures was shown to promote the regeneration of hyaline cartilage. The molecular mechanisms by which chitosan promotes cartilage regeneration remain unknown. Because neutrophils are transiently recruited to the microfracture site, the effect of 80% deacetylated chitosan on the function of neutrophils was investigated. Most studies on neutrophils use preparations of chitosan with an uncertain degree of deacetylation. For therapeutic purposes, it is of interest to determine whether the degree of deacetylation influences the response of neutrophils to chitosan. The effect of 95% deacetylated chitosan on the function of neutrophils was therefore also investigated and compared with that of 80% deacetylated chitosan. 相似文献57.
This study investigates the thermal activity thresholds of the predatory mirid Nesidiocoris tenuis Reuter (Hemiptera: Miridae) and two spotted spider mite Tetranychus urticae Koch (Acari: Tetranychidae). Adult N. tenuis lost locomotory function and entered chill coma at significantly lower temperatures (4.0°C and 0.3°C, respectively) than adult T. urticae (7.0°C and 5.7°C, respectively). However, the mirids were more adversely affected by high temperatures, with T. urticae losing the ability to walk and entering heat coma at higher temperatures (47.3°C and 49.7°C, respectively) than N. tenuis (43.5°C and 46.6°C, respectively). Across a range of temperatures (2.5–20°C) adult N. tenuis had faster walking speeds than T. urticae. These data are discussed in relation to the climatic conditions under which N. tenuis would be an effective biocontrol agent. 相似文献
58.
59.
Wirschell M Yamamoto R Alford L Gokhale A Gaillard A Sale WS 《Archives of biochemistry and biophysics》2011,510(2):4527-100
Recent evidence has revealed that the dynein motors and highly conserved signaling proteins are localized within the ciliary 9 + 2 axoneme. One key mechanism for regulation of motility is phosphorylation. Here, we review diverse evidence, from multiple experimental organisms, that ciliary motility is regulated by phosphorylation/dephosphorylation of the dynein arms through kinases and phosphatases that are anchored immediately adjacent to their axonemal substrates. 相似文献
60.
John Llewelyn Ben L. Phillips Greg P. Brown Lin Schwarzkopf Ross A. Alford Richard Shine 《Evolutionary ecology》2011,25(1):13-24
Biological invasions can expose native predators to novel prey which may be less nutritious or detrimental to predators. The introduction and subsequent spread of cane toads (Bufo marinus) through Australia has killed many anuran-eating snakes unable to survive the toad’s toxins. However, one native species, the keelback snake (Tropidonophis mairii), is relatively resistant to toad toxins and remains common in toad-infested areas. Is the keelback’s ability to coexist with toads a function of its ancestral Asian origins, or a consequence of rapid adaptation since cane toads arrived in Australia? And does the snake’s feeding preference for frogs rather than toads reflect an innate or learned behaviour? We compared keelback populations long sympatric with toads with a population that has encountered toads only recently. Unlike toad-vulnerable snake species, sympatry with toads has not affected keelback toxin tolerances or feeding responses: T. mairii from toad-sympatric and toad-naïve populations show a similar sensitivity to toad toxin, and a similar innate preference for frogs rather than toads. Feeding responses of neonatal keelbacks demonstrate that learning plays little or no role in the snake’s aversion to toads. Thus, behavioural aversion to B. marinus as prey, and physiological tolerance to toad toxins are pre-existing innate characteristics of Australian keelbacks rather than adaptations to the cane toad’s invasion of Australia. Such traits were most likely inherited from ancestral keelbacks that adapted to the presence of bufonids in Asia. Our results suggest that the impact of invasive species on native taxa may be strongly influenced by the biogeographic histories of the species involved. 相似文献