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21.
Juliette Gaëtan Sébastien Halary Maxime Millet Cécile Bernard Charlotte Duval Sahima Hamlaoui Amandine Hecquet Muriel Gugger Benjamin Marie Neha Mehta David Moreira Fériel Skouri-Panet Cynthia Travert Elodie Duprat Julie Leloup Karim Benzerara 《Environmental microbiology》2023,25(3):751-765
The formation of intracellular amorphous calcium carbonates (iACC) has been recently observed in a few cultured strains of Microcystis, a potentially toxic bloom-forming cyanobacterium found worldwide in freshwater ecosystems. If iACC-forming Microcystis are abundant within blooms, they may represent a significant amount of particulate Ca. Here, we investigate the significance of iACC biomineralization by Microcystis. First, the presence of iACC-forming Microcystis cells has been detected in several eutrophic lakes, indicating that this phenomenon occurs under environmental conditions. Second, some genotypic (presence/absence of ccyA, a marker gene of iACC biomineralization) and phenotypic (presence/absence of iACC) diversity have been detected within a collection of strains isolated from one single lake. This illustrates that this trait is frequent but also variable within Microcystis even at a single locality. Finally, one-third of publicly available genomes of Microcystis were shown to contain the ccyA gene, revealing a wide geographic and phylogenetic distribution within the genus. Overall, the present work shows that the formation of iACC by Microcystis is common under environmental conditions. While its biological function remains undetermined, this process should be further considered regarding the biology of Microcystis and implications on the Ca geochemical cycle in freshwater environments. 相似文献
22.
Environmental gradients in a southern Europe estuarine system: Ria de Aveiro,Portugal implications for soft bottom macrofauna colonization 总被引:4,自引:0,他引:4
Four seasonal sampling surveys were carried out between December 1985 and September 1986 in Canal de Mira (Ria de Aveiro,
Portugal). A total of 40 sampling stations, distributed over 13 transects, was used. Salinity, temperature, dissolved oxygen
and pH of the water mass were measured. Sediment temperature, and salinity and pH of interstitial water were determined. Sediment
variables also included granulometric composition and organic matter contents. Bottom macrofauna samples were collected at
each station.
Ordination (PCA and MDS) and classification of the sampling stations were performed, using the physicochemical and the biological
data sets separately. Average linkage cluster analysis using the unweighted paired-group method, arithmetic averages, was
used for both sets of data.
With a salinity range from 35.1‰ to 0.0‰, Canal de Mira behaves like a tidally and seasonally poikilohaline estuary. Water
temperature (8.5–24.7°C) decreased along the channel towards its inner part during the cold season; an inverse and more pronounced
trend was observed during the hot season. Dissolved oxygen contents was generally high during the day (50% to 240% saturation).
Oversaturation was observed throughout the growing season, with peaks in areas with large amounts of rooted vegetation. The
pH values, largely correlated with dissolved oxygen, ranged from 6.8 to 8.9. Four types of sediment were present in Canal
de Mira, medium and muddy sands being dominant.
Two major gradients were identified: (i) a typical longitudinal estuarine gradient, associated with distance from the mouth,
representing physicochemical variables such as tidal amplitude, salinity and temperature; this gradient was accompanied by
an upstream increase in dominance; the community composition changes were mainly related to salinity; (ii) a lateral gradient,
related to current velocity, depth and sediment composition; the subtidal community had a comparatively low species richness
and abundance. Groups of stations could be recognized along the environmental gradients. Benthic community changes, however,
appeared to be gradual rather than marked by abrupt transitions. 相似文献
23.
24.
Sophie L. Richards Harith Farooq Hermenegildo Matimele Tereza Alves Castigo Datizua Clayton Langa Alice Massingue Jo Osborne Saba Rokni Camila de Sousa Iain Darbyshire 《Biotropica》2023,55(6):1183-1194
Successful protected area networks must represent biodiversity across taxonomic groups. However, too often plant species are overlooked in conservation planning, and the resulting protected areas may, as a result, fail to encompass the most important sites for plant diversity. The Mozambique Tropical Important Plant Areas project sought to promote the conservation of Mozambique's flora through the identification of Important Plant Areas (IPAs). Here, we use the Weighted Endemism including Global Endangerment (WEGE) index to identify the richest areas for rare and endemic plants in Mozambique and subsequently evaluate how well represented these hotspots are within the current protected area and IPA networks. We also examine the congruence between IPA and protected areas to identify opportunities for strengthening the conservation of plants in Mozambique. We found that high WEGE scores, representing areas rich in endemic/near-endemic and threatened species, predict the presence of IPAs in Mozambique, but do not predict the presence of protected areas. We also find that there is limited overlap between IPAs and protected areas in Mozambique. We demonstrate how IPAs could be an important tool for ensuring priority sites for plant diversity are included within protected area network expansions, particularly following the adoption of the “30 by 30” target agreed within the post-2020 Convention on Biological Diversity framework, with great potential for this method to be replicated elsewhere in the global tropics. 相似文献
25.
Varilla González Jean David Macedo Alves Flávio Bagnatori Sartori Ângela Lúcia de Oliveira Arruda Rosani do Carmo 《Journal of plant research》2023,136(4):453-481
Journal of Plant Research - The Pterocarpus clade includes 23 genera previously attributed to different Fabaceae tribes. The recent rearrangements of many genera in the clade do not recognize... 相似文献
26.
27.
Operational patterns affecting lactic acid production in ultrafiltration cell recycle bioreactor 总被引:2,自引:0,他引:2
Lactic acid production with cell recycling on an ultrafiltration tubular membrane reactor was studied; higher lactic acid concentrations as well as productivities were obtained under long-term fermentations compared with other high cell density systems. Different operational conditions, namely dilution rates and start-up modes, were assessed. Performances were very different at the three different dilution rates tested (D = 0.20 h(-1), D = 0.40 h(-1), or D = 0.58 h(-1)). The different behaviours are discussed and factors responsible for them are presented. The best way to operate for lactic acid production is chosen, the dilution rate of D = 0.40 h(-1) being the one providing the best overall performance. On the other hand, results show that of the two start-up modes tested, continuous start (membrane open) permits higher permeabilities throughout the operational runs than batch start (membrane closed). Operational stability was found to be directly associated with membranes that work at "steady state," the membrane permeability being kept around 15 L/m(2) h. Optimized cell bleed can improve time of operation if such membrane permeability can be maintained for a longer time. A comparison of results with those obtained in other lactic acid production systems is presented; such comparison shows that this tubular ultrafiltration membrane cell recycle reactor presents three important advantages: (1) concomitant lactic acid concentrations and productivities; (2) long periods of operation at reasonable permeabilities; and (3) good mechanical stability permitting the use of steam sterilization. (c) 1995 John Wiley & Sons, Inc. 相似文献
28.
Baudin Bruno; Alves Nathalie; Pilon Antoine; Beneteau-Burnat Benedicte; Giboudeau Jacqueline 《Glycobiology》1997,7(4):565-570
We enzymatically deglycosylated pig lung angiotensin I-convertingenzyme (ACE) to study the involvement of its glycanic chainsin its physicochemical and catalytic properties. The effectsof endoglycosidases F2 and H, and of N-glycanase were assessedby ACE mobility in SDS-PAGE. N-Glycanase only was completelyeffective with or without previous denaturation, leading toa shift in ACE Mr from 172 to 135 kDa; endoglycosidase F2 producedthe same shift but only without previous denaturation. DeglycosylatedACE had the same kcat as native ACE for the substrate hippuryl-histidyl-leucine,and an identical Stokes radius as measured by size-exclusionhigh performance liquid chromatography. Neuraminidase had noeffect on ACE Stokes radius but slightly decreased its kcatwhich could be related to variations in ionization of the activesite. The isoelectric point of ACE, as, determined by isoelectricfocusing, increased from 4.54.8 to 5.05.3 aftereither endoglycosidase F2 or neuraminidase digestion, but stillwith microheterogeneities which thus did not seem to be relatedto ACE glycans. Deglycosylated ACE did not bind onto agaroselectinsin contrast to native ACE which bound strongly to concanavalinA showing interactions involving oligomannosidic or biantennaryand sialylated N-acetyl-lactosaminic isoglycans. Finally, tunicamycin,an inhibitor of N-glycosylation, did not modify ACE secretionby endothelial cells. Thus, ACE glycans have no drastic effectson structural and biological properties of the protein, butthey may have a functional role on intracellular targeting ofboth secreted and membrane-bound ACE isoforms, also for theprotection of the soluble plasma form against hepatic lectinsand the maintenance of its hydrosolubility. converting enzyme (peptidyldipeptidase EC.3.4.15.1) endothelium glycosidases lectins 相似文献
29.
Dr J. O. Lopes D. V. Pereira L. A. Streher A. A. Fenalte S. H. Alves J. P. Benevenga 《Mycopathologia》1995,130(2):89-92
A case of cutaneous zygomycosis caused byAbsidia coryabifera in a leukemic patient submitted to chemotherapy is reported. The lesion was located on the little finger of the right hand and probably resulted from a latent osteomyelitis. It progressed to form extensive necrotic area. No systemic infection was detected and the lesion did not appear to be associated with any trauma. 相似文献
30.
Thirty-four strains belonging to various species of the genus Thermus (T. aquaticus, "T. thermophilus,"
"T. brockianus,"
T. scotoductus, and genomic species 2) isolated from hot springs of different geographical areas were examined for plasmid content and restriction
fragment length polymorphism (RFLP) of plasmid DNAs. The four strains of the numerical taxonomy cluster E of genomic species
2 did not harbor plasmid DNA. Overall examination of the HindIII-RFLP profiling of plasmid DNA showed considerable variability between and within genomic species, with the exception
of presumed clonal isolates. In spite of this heterogeneity, HindIII plasmid digests within a numerical taxonomic cluster gave a subset of restriction fragments of similar or identical length.
Strains belonging to genomic species 2 or unclassified isolates from S. Pedro do Sul that harbored plasmid DNA (7 of the 14
strains studied) exhibited strong DNA homology between plasmid regions. No homologous sequences to these plasmid regions were
found in chromosomal DNA from strains isolated from S. Pedro do Sul in which no plasmids were detected. The strains belonging
to T. scotoductus formed two plasmid DNA homology groups, as estimated by probing with a plasmid fragment that coincided with the two numerical
taxonomy clusters proposed previously. Among the other species, homology of plasmid regions was also found between some strains.
Strong homology was also found between plasmid regions from some strains of different taxonomic groups, isolated from the
same and from different sources, suggesting that these sequences are highly conserved in plasmids present in Thermus. For plasmid-containing strains, results of plasmid RFLP profiling/DNA homology appear promising for the typing of Thermus at the level of biotypes or of individual strains, namely, for monitoring the diversity and frequency of isolates from a
particular hot spring.
Received: 24 October 1994 / Accepted: 6 March 1995 相似文献