《生命的化学》征稿启事

１、《生命的化学》是中国生物化学与分子生物学会的会刊和通讯，是国内外公开发行的综述性、通讯性的生物化学与分子生物学期刊，重点刊登综述性、进展性、报道性文章，同时也旁及技术和方法、生物化学与分子生物学家的传记轶事、书评、教学讨论、访问观感等内容。《生命...     

我国主要地方绵羊品种mtDNA D-loop区PCR-RFLP研究

利用5种限制性内切酶（Hinf I,Msp I,Sau3A I,Xsp I,Taq I）,采用PCR-RFLP技术研究了我国9个地方绵羊品种以及2个引入品种共计83只绵羊个体线粒体DNA D-loop区的多态性。结果表明,我国主要地方绵羊品种线粒体DNA D-loop区存在两种基本单体型,提示我国主要地方绵羊品种起源于两个母系祖先。线粒体DNA D-loop区多态度为0.042 1%,说明我国地方绵羊品种线粒体DNA多态度较为贫乏。
     

安徽和黑龙江省大豆疫霉群体遗传结构的SSR分析

采用简单重复序列(SSR)的分析方法,对来自安徽和黑龙江省的大豆疫霉群体进行了遗传多样性分析。通过使用20对SSR引物对供试的83株大豆疫霉菌株进行PCR扩增,共得到109个SSR标记,全部为多态性标记,平均每对引物扩增出5.5条带。遗传变异与相似性分析表明,安徽群体具有更高的遗传变异度,安徽群体与黑龙江群体间遗传相似性较低;聚类分析显示,供试菌株在80%的相似性水平上可被区分为7个类群,且安徽群体分布于更多的聚类组中;Shannon-Wiener多样性指数也表明安徽群体的遗传多样性较黑龙江群体丰富。综合分析表明,本研究的结果不支持关于安徽的大豆疫霉可能来源于黑龙江的推测。     

温度对橄榄蛏蚌耗氧和氨氮排泄的影响

The respiration metabolismand excretion of marinebivalves were studied by different researchers[1—6].Themetabolic rate of bivalves is influenced by a number ofvariables,includingtemperature,body size,oxygen ten-sion,food concentration,reproductive state,activityleveland physiological condition.The excreted metabolites ofbivalves include ammonia,urea,uric acid and others,with ammonia comprising70%of the total excretion.Solenaia oleivorais a proper freshwater bivalve in China.For the consumer it has the follo...     

香溪河水体中生源要素磷的时空分布研究

Phosphorus is an essential and oftenlimiting nutrientin both marine andfreshwater ecosystems,yetits oversup-plyis of concern in many environments due to its role ineutrophication[1].Phosphorus enters rivers from diffusecatchment sources(particularly agriculture)and point(effluent)sources.However,river systems have an im-portant internal capacity to remove or release phosphorusfrom/to the water column and to trans-form phosphorusbetween organic,inorganic,particulate and dissolvedforms.River bed…     

蝎毒多肽提取物抑制DU- 145 细胞 COX- 2 和MMP- 9 表达的研究

目的：研究蝎毒多肽提取物（peptide extract from scorpion venom,PESV）对雄激素非依赖性人前列腺癌细胞株DU-145COX-2和MMP-9表达的影响,进一步探讨其抗血管生成的分子机制,为抗前列腺癌骨转移提供有效的治疗手段。方法：采用免疫组只化学方法检测PESV对COX-2、MMP-9蛋白表达的影响,应用RT-PCR检测PESV对MMP-9在mRNA水平表达的影响。结果：蝎毒多肽提取物（40μg／mL）作用于前列腺癌细胞后,COX-2、MMP-9蛋白表达水平明显下调（P〈0．05）,进一步检测发现MMP-9在mRNA水平亦明显下降（P〈0．05）。结论：蝎毒多肽提取物（PESV）通过抑制前列腺癌细胞血管生成因子COX-2的表达而发挥其抗血管生成作用,具有临床应用价值。     

Effects of ELF magnetic fields on protein expression profile of human breast cancer cell MCF7

A case-control study in 1979 on electrical wiring configurations and childhood leukemia had stimulated interest in the issue that extremely low frequency magnetic fields (ELF MF) may have harmful biologi-cal effects especially on the incidence of human can-cer[1]. Since then, a large number of studies have been conducted to follow up this important result[2]. The majority of these studies indicate a weak association between exposure to 50―60 Hz ELF MF and the in-cidence of cancer; however…     

Silver stained polyacrylamide gels and fluorescence-based automated capillary electrophoresis for detection of amplified fragment length polymorphism patterns obtained from white-rot fungi in the genus Trametes

Silver stained denaturing polyacrylamide gels (PAGEs) and fluorescent denaturing automated capillary electrophoresis (CE) were used to detect amplified fragment length polymorphism (AFLP) patterns obtained from white-rot fungi belonging to the genus Trametes. AFLP fingerprinting detected by the fluorescence-based method as well as by silver staining showed a high discriminatory power in differentiating nine strains of Trametes ochracea, nine strains of Trametes hirsuta and ten isolates of Trametes versicolor. UPGMA dendrograms derived from fluorescently labelled and silver stained AFLP patterns were similar, but a few differences were detected especially in the clustering of T. ochracea and T. hirsuta strains. Compared to silver-stained AFLP, detection of fluorescent AFLP was fast, reliable and easy to perform and it facilitated surveying with a computerized analysis system. Fluorescent CE seems to be well suited for studying similarity between Trametes species.