刺槐根瘤菌的研究——Ⅰ菌种分离筛选、形态及生理特性

作者从干旱地区刺槐根瘤中选出6株固氮力高、抗逆性强的根瘤菌,并研究了其形态特征及主要生理特性,发现刺槐根瘤菌生长速度快,代谢过程产酸,有较高的耐盐力,广泛的pH生长范围及较高的离体固氮活性,有次端生和周生鞭毛.此研究结果为刺槐根瘤菌分类及林业上的应用提供资料和依据。     

链球菌属的一个新种

自君子兰(Clivia miniata)软腐病病株的叶片病灶中分离到两株致病菌。经鉴定其中一株(85—2)多为双球状,也有呈单个存在,极少为四个细胞相连的短链。无芽孢,无鞭毛,不运动,革兰氏染色阳性,抗酸染色阴性,兼性厌氧生长;可从多种糖类产酸,但不产气;接触酶和苯丙氨酸脱氨酶均为阴性;不液化明胶,还原石蕊牛奶并凝固、胨化;生长温度范围为15—53℃,DNA中G+C含量为41．7mol％。由于其性状与链球菌属的各已知种均不相同,故定为一个新种,命名为君子兰链球菌(Streptococcus miniatus Zhou,Ping et Shao sp. Nov.)。     

家兔中脑导水管周围灰质中注射八肽胆囊收缩素（CCK—8）拮抗吗啡镇痛和...

We have reported that intracerebroventricular (i. c. v.) injection of 1-4 ng of CCK-8 to the rat produced a remarkable antagonistic effect on morphine analgesia. In order to study the species specificity and the site of action, CCK-8 was microinjected into the PAG of the rabbit, and its influence on morphine analgesia and electroacupuncture analgesia was observed. The latency of the escape response (ERL) to radiant heat focused on the snout was measured as an index of the pain threshold. Microinjections were made via cannulae chronically implanted into the PAG. The drug solutions were delivered in a volume of 1 microliter, at a speed of 0.125 microliter/min. The ERL was measured for a period of 60 or 70 minutes at 10 min intervals. 1. CCK-8 administered unilaterally to the PAG of the rabbit at a dose of 3 ng antagonized the analgesia induced by morphine (4 mg/kg, i. v.) by 73% (P less than 0.001), and reduced the analgesic effect of electroacupuncture by 67% (P less than 0.001). These effects were dose-dependent within the range from 1.5 ng to 6.0 ng. The effect of CCK-8 was reversed by CCK receptor blocker proglumide (4 microliters, intra-PAG injection). Unsulfated CCK-8 (CCK-us) had no effect in this regard. These results indicate that in the PAG of the rabbit, exogenously administered CCK-8 was capable of antagonizing opioid analgesia by the activation of CCK receptors. 2. Two groups of rabbits were given with morphine (2 mg/kg, i. v.) and simultaneous injection of CCK-8 antiserum (CCK-AS, 1 microliter) or normal rabbit serum (NRS) into the PAG.(ABSTRACT TRUNCATED AT 250 WORDS)     

根结线虫天敌真菌的筛选研究初报

从土壤和病株上分离到10个线虫的天敌真菌菌株,其中有三个菌株较有希望,烛台霉属(Candelabrello sp.)一个。孤孢属(Monacrosporium spp.)两个,菌株代号分别为CN_7;CN_(?)和CN_5。 据试验:CN_7较好,菌丝体生长的温度范围是20～33℃,最适宜的温度范围是25～30℃,以式捕捉环捕食线虫,对培养基选择性不强,最适宜的pH值是6.0～7.5,但在pH4.5～8.0都能进收缩行捕捉,捕捉器官形成量多,捕虫势强且稳定,在水中也能形成捕捉环并捕食线虫。     

抑菌试验用于测定T-2毒素

本文从镰刀菌中提取T-2毒素,对不同的酵母菌进行抑菌试验,结果证明红酵母属(Rho-dotorula)的三个种,即红酵母(R.glutrnis)、深红酵母(R.ruber)和小红酵母(R.minuta)对T-2毒素最敏感,被定为测毒敏感菌。将抑菌试验与家兔皮肤反应试验、豌豆发芽抑制试验进行对比,结果有部分重合,但不能相互取代。由于抑菌试验具有快速、简便、准确的优点,可做为测定T-2毒素的方法之一。     

试论云阳县人口激增与土地锐减的矛盾及对策

土地是人们赖以生存的物质基础,是其它任何东西不可代替的生产资料。离开了土地,人就不能生存下去,这是众所周知的真理。但是,目前人口的激增与土地锐减的矛盾越来越尖锐。据有关统计资料表明,1978—1988年全国耕地面积减少3.66×10~6ha,与此同时全国人口又猛增1.33亿,给农业粮食生产、