Effects of antibiotics on modern chronic diseases

The discovery of antibiotics is the miracle of modern medicine,which has contributed greatly to the protection of human health.However,in despite of medical advances to date,some quite inexplicable modern diseases are still haunting us.These chronic diseases are less fierce in fatality,but more permanently compromise the quality of patients′lives.In this paper,the relevant background knowledge about antibiotics were introduced and the relationship between antibiotics and several common chronic diseases was analyzed,in order to promote the rational use of antibiotics and the correct view on antibiotics.     

Benzene containing polyhydroxyalkanoates homo- and copolymers synthesized by genome edited Pseudomonas entomophila

Microbial synthesis of functional polymers has become increasingly important for industrial biotechnology.For the first time,it became possible to synthesize controllable composition of poly(3-hydroxyalkanoate)(P3HA)consisting of 3-hydroxydodecanoate(3HDD)and phenyl group on the side-chain when chromosome of Pseudomonas entomophila was edited to weaken itsβ-oxidation.Cultured in the presence of 5-phenylvaleric acid(PVA),the edited P.entomophila produced only homopolymer poly(3-hydroxy-5-phenylvalerate)or P(3HPhV).While copolyesters P(3HPhV-co-3HDD)of 3-hydroxy-5-phenylvalerate(3HPhV)and 3-hydroxydodecanoate(3HDD)were synthesized when the strain was grown on mixtures of PVA and dodecanoic acid(DDA).Compositions of 3HPhV in P(3HPhV-co-3HDD)were controllable ranging from 3%to 32%depending on DDDA/PVA ratios.Nuclear magnetic resonance(NMR)spectra clearly indicated that the polymers were homopolymer of P(3HPhV)and random copolymers of 3HPhV and 3HDD.Their mechanical and thermal properties varied dramatically depending on the monomer ratios.Our results demonstrated the possibility to produce tailor-made,novel functional PHA using the chromosome edited P.entomophila.     

Effects of Temperature and pH on Cell Growth of a Flocculating Microalga (Chlorella vulgaris) JSC-7

微藻是可广泛用于健康食品及水产养殖的饵料,同时,微藻细胞内积累的油脂可作为可再生生物燃料,因此微藻的生长和代谢受到广泛关注。温度和pH对微藻的生物量积累有很大影响,考察不同温度和pH条件下微藻细胞的生长有助于寻找最佳的条件进行微藻的培养。自絮凝小球藻JSC-7（Chlorella vulgaris JSC-7）可实现自沉降采收,有利于降低微藻生产成本,优化其生长条件对更好地利用该微藻具有重要意义。考察了温度（22∽40℃）及pH（6.0∽10.0）对其细胞生长、叶绿素含量和油脂产量的影响。在所选取的温度及pH范围内,JSC-7细胞均可生长,显示该藻种可以适应广泛的温度和pH条件。适合细胞生长的温度依次为31℃〉28℃〉35℃〉25℃,pH依次为7.0〉8.0〉6.0。pH 8.0时生物量和油脂的积累量最多,说明该藻株在弱碱条件下更适合生长和产油。当温度为31℃、pH为7.0时,可获得最高的生长量（OD690=0.941）、叶绿素含量（19 mg/L）及油脂产量（39.07%/克干重）。     

Using a non-radioisotopic, quantitative TRAP-based method detecting telomerase activities in human hepatoma cells

INTRODUCTIONTelomerase is a ribonucleoprotein complex that plays a critical role in telomeremaintenance and cellular immortality. Telomerase has been considered as tumordiagnostic marker and potential target for cancer therapyll, 2]. A sensitive, reliableand quantitative assay is of high interest in this field. The development of a very sensitive Telomeric Repeat Amplification Protocol (TRAP) for measuring telomerajseactivity in cell extracts has been proved to be an important tool for…     

糖尿病患者指纹白线的分析

作者对298例糖尿病患者的指纹白线的出现率进行了调查,结果表明,糖尿病患者的指纹白线出现率明显高于同一地区正常人群,差异高度显著(P<0 .01)。其中女性指纹白线的出现率明显高于男性,差异显著(P<0.05),较某些遗传病指纹白线的出现率低。     

Functional expression of opioid receptor—like receptor and its endogenous specific agonist nociceptin／orphanin FQ during mouse embryogenesis

INTRODUCTIONOpioidreceptorsbelongtotheG-protein-coupledreceptorfamilythatischaracterizedbytheseventransmembranespanningdomainsinstructure.Threesubtypesoftheopioidreceptors(H,6,andK)havebeenclonedandcharacterizedthroughtheir1.CorrespondingauthorFunctionalexpressionofORLIandN/OFQduringmouseembryogenesisdistinctaffinitiesfordifferentopioidligands.TheseopioidreceptorsareallcoupledtotheinhibitoryGprotein(Gi)andnegativelyregulateadenylatecyclase[1].Opioidreceptor--likereceptor(ORLI),anew…     

Human chromosome pellicle antibody recognizing centromere protein-C (CENP-C), the main component of the kinetochore

INTRODUCTIONDuringnormalcelldivision,thechromosomesplitsevenly,givingthesamegeneticmaterialtoeachdaughtercell.Theaccuracyofsuchcelldivisiondependsontheinteractionbetweenthespindleapparatusandtheets--actingregionofeachchromosomeknownasthecentromere.Manyessentialmitoticfunctionsoccuratorarecontrolledbythecentromere.Capturingspindlemicrotubules,thegrowthanddisassemblyofmicrotubules,theliningupofchromosomesonthemetaphaseplate,andthesplittingoffofthesisterchromatidsduringmitosisareallsomehowre…     

寻常型银屑病遗传度的研究

对临床已确诊的212例寻常型银屑病患者进行了一、二、三级亲属发病率调查, 结果表明,患者一级亲属的发病率为3.8%,对照组一级亲属发病率为0.42%,本病的遗传方式为多基因遗传,遗传度为58.2%。     

三宝鸟核型的研究

三宝鸟(Eurystomus orientalis Linnaeus)属于佛法僧目(Coraciiformes)的佛法僧科(Coraciidae)。主要分布于日本南部、印度、中印半岛、澳洲以及我国的大部分地区。本文分析了分布于我国三宝鸟的染色体核型,试图从核型上了解该物种的特性,并初步探讨其演化程度和分类地位。