miR-15a 和miR-16-1 模拟物对人骨肉瘤细胞系SOSP-9607 凋亡 和增殖的影响

目的:探讨miR-15a和miR-16-1模拟物对于人骨肉瘤细胞系SOSP-9607凋亡和增殖的影响。方法:将SOSP-9607细胞分为实验组和对照组。实验组分为miR-15a组、miR-16-1组、miR-15a+miR-16-1组。以miR-15a组为例,采用miR-15a模拟物(hsa-miR-15a mimics)上调SOSP-9607细胞内的miR-15a表达量。对照组分为阴性对照组和空白对照组。采用流式细胞仪测定细胞凋亡率,四甲基偶氮唑蓝(MTT)法测定细胞增殖,并计算细胞增殖效率。结果:通过统计学分析,实验组凋亡率与阴性对照组凋亡率相比明显增高(P<0.05);实验组的细胞增殖率明显低于对照组(P<0.05)。结论:上调SOSP-9607细胞内miR-15a和miR-16-1的表达量可促进SOSP-9607细胞的凋亡并抑制其增殖。     

分离自石果衣的共生菌藻在干燥饥饿胁迫下存活能力分析

对分离自地衣石果衣的共生菌藻在干燥和饥饿胁迫下的存活能力进行了分析.对其中的共生菌进行了干燥和饥饿双胁迫以及饥饿单胁迫两种实验处理,对共生藻进行了干燥单胁迫实验处理.实验通过使用扫描电子显微镜、透射电子显微镜、荧光显微镜、分光光度法和流式细胞术对生物量大小、重量和细胞密度、菌丝和藻细胞畸形以及代谢活力进行了检测.结果显示,共生菌在干燥和饥饿双胁迫下能存活7个月;在饥饿单胁迫下能存活8个月.共生藻在干燥胁迫下能存活2个月.本实验为深入研究地衣繁殖生物学和固沙固碳的干旱沙漠生物地毯工程提供了科学依据.     

CHEK2*1100delC and susceptibility to breast cancer: a collaborative analysis involving 10,860 breast cancer cases and 9,065 controls from 10 studies

Previous studies of families with multiple cases of breast cancer have indicated that a frameshift alteration in the CHEK2 gene, 1100delC, is associated with an elevated frequency of breast cancer in such families, but the risk associated with the variant in other situations is uncertain. To evaluate the breast cancer risk associated with this variant, 10,860 breast cancer cases and 9,065 controls from 10 case-control studies in five countries were genotyped. CHEK2*1100delC was found in 201 cases (1.9%) and 64 controls (0.7%) (estimated odds ratio 2.34; 95% CI 1.72–3.20; P=.0000001). There was some evidence of a higher prevalence of CHEK2*1100delC among cases with a first-degree relative affected with breast cancer (odds ratio 1.44; 95% CI 0.93–2.23; P=.10) and of a trend for a higher breast cancer odds ratio at younger ages at diagnosis (P=.002). These results confirm that CHEK2*1100delC confers an increased risk of breast cancer and that this risk is apparent in women unselected for family history. The results are consistent with the hypothesis that CHEK2*1100delC multiplies the risks associated with susceptibility alleles in other genes to increase the risk of breast cancer.     

贵州梵净山地衣的初步研究(英文)

梵净山国家级自然保护区是联合国“人与生物圈网”的成员之一,位于中国西南贵州省的东北部。报道了梵净山地衣物种119种,其中106种为梵净山新记录,79种为贵州新记录,2种为中国新记录:Dermatocar-ponintestiniforme(K rb.)Hasse.和Thelotrema similansNylander,同时对梵净山地衣进行了初步的地理学分析,从而为该地区地衣生物多样性的研究及地衣资源的保护和利用提供了相关资料。     

山西桑干河流域湿地植被优势种群分布格局研究

根据野外调查的样地资料,应用方差/均值比（扩散系数）的t检验、聚集指数、平均拥挤度、聚块性指数、Green指数和负二项分布的聚集强度等方法,研究了山西桑干河流域湿地植被优势种群的分布格局并分析格局形成的原因,并用相关分析比较了6个指数间的关系,结果表明：这些植物的分布格局皆属于聚集分布,但它们的聚集强度有差异。聚集强度最大的是沙棘和柽柳,聚集强度最小的是小车前和车前,由于它们均分布于河漫滩或者河床地势较高处,土壤水分含量较低,仅仅在洪水季节短时间有积水存在,不是典型的湿地植物。典型的水生植物泽泻、莎草、轮藻、芦苇、香蒲、慈姑,湿生植物水葱、藨草等,它们的聚集强度居中,对水分有着较强的依赖性,均分布于流水较缓的河道,或者濒临河道的积水处。此外,多年生的莎草、芦苇、香蒲、水葱、藨草等主要依靠无性繁殖扩大种群分布范围和密度,这也是它们服从聚集分布的原因之一。本研究还说明方差/均值比率法是一种较好的分析种群分布格局的方法。     

濒危植物南方红豆杉濒危原因分析

南方红豆杉是我国第三纪孑遗特有树种,为国家1级濒危保护植物,主要分布于在长江流域、南岭山脉以及广东、广西、江西、河南、陕西、甘肃、台湾和山西等省（区）的山地。南方红豆杉分布区的生态环境特征是：热量充足,温暖潮湿。南方红豆杉濒危的主要原因有：（1）种子产生量少,萌发率和幼苗成活率较低（2）种群缺乏较强的竞争力,（3）地理分布的局限性,（4）对生境要求的特殊性和（5）人为破坏严重。此外,针对南方红豆杉濒危现状和原因,还提出了科学保护和可持续利用的对策。     

In vitro Anti-viral Activity of the Total Alkaloids from Tripterygium hypoglaucum against Herpes Simplex Virus Type 1

Herpes simplex virus type 1 (HSV-1) is a commonly occurring human pathogen worldwide. There is an urgent need to discover and develop new alternative agents for the management of HSV-1 infection. Tripterygium hypoglaucum (level) Hutch (Celastraceae) is a traditional Chinese medicine plant with many pharmacological activities such as anti-inflammation, anti-tumor and antifertility. The usual medicinal part is the roots which contain about a 1% yield of alkaloids. A crude total alkaloids extract was prepared ...     

尼古丁调控细胞凋亡的分子机制

尼古丁是烟草中生物碱的主要成分,其生物学作用广泛。尼古丁参与影响神经系统、呼吸系统和心血管系统等重要器官的发育,并与癌症的发生有着密切的关系。尼古丁通过对细胞凋亡的调控,发挥其生物学作用。现对尼古丁调控细胞凋亡相关的各种信号通路及其分子机制进行综述。     

合欢花对慢性应激大鼠生长和脑单胺类神经递质含量的影响

为探讨合欢花对慢性应激大鼠生长和脑单胺类神经递质的影响,采用15只大鼠,设置了对照组、应激组和合欢花组3组实验。应激组和合欢花组均接受7天的应激刺激,之后合欢花组再灌胃合欢花10天。实验结束后,取3组大鼠的脑组织,用高效液相色谱法测定高香草酸(HVA)、去甲肾上腺素(NE)、多巴胺(DA)和5-羟色胺(5-HT)的含量。结果表明,应激组大鼠日增重显著低于对照组(P=0.011);而合欢花组大鼠的日增重极显著高于应激组(P=0.002)。应激组大鼠海马、纹状体和前额叶中的HVA含量与对照组相比,虽有升高的趋势,但无显著差异;两组间的NE、DA和5-HT也无显著差异。合欢花组大鼠海马中的HVA、DA含量明显高于应激组,而前额叶中的多巴胺和5-羟色胺,以及纹状体中的5-羟色胺均明显低于应激组。这表明合欢花对慢性应激引起的大鼠生长受抑有缓解作用,对其脑内单胺类神经递质有调节作用。     

Selective gene therapy for human lung adenocarcinoma by tumor-specific expression of herpes simplex virus thymidine kinase gene

According to the fact that CEA gene expressed only in lung adenocarcinoma but not in normal lung cells, a retroviral expression vector (pCEATK) of the herpes simplex virus thymidine kinase (HSV-TK) gene regulated by CEA promoter was constructed and introduced into CEA-producing human lung adenocarcinoma cells GL and non-CEA-producing HeLa cells. The expression of pCEATK and Ganciclovir (GCV) sensitivity of the transfected cells were tested in vitro and in vivo . pCEATK expressed only in CEA-producing GL cells but not in non-CEA-producing HeLa cells. The sensitivity to GCV of pCEATK-transfected GL was 992 times higher compared with that of the parental cell line and there was obvious "bystander effect" in vitro. HeLa cells transfected wtih pCEATK were still resistant to GCV. Injection of GCV resulted in significant regression of pCEATK-transfected GL tumors in nude mice. In addition, all mice with any fraction of GL cells expressing HSV-TK exhibited a significant reduction in tumor growth, including mice