用直接涂片检查快速诊断弯曲菌性肠炎

<正>空肠弯曲菌是急性胃肠炎常见的病原体。最近已证实,空肠弯曲菌引起的感染多见于夏季,其发生率甚至多于常见的诸如沙门氏菌属和志贺氏菌属的肠道致病菌。 常规诊断方法需要2～4天才能出结果。粪便标本立即接种选择培养基(如弯曲菌血平板——Campy-BAP)上或接种含抗生素的巯基醋酸盐培养基中,中加有CO_2的37℃温箱中培育24～48小时,挑取可疑菌落作鉴别试验后才能确诊。 过去,粪便标本涂片企图用革兰氏染色法给弯曲菌性肠炎作快速诊断,但敏感性较低。     

朝鲜木本糖料植物考察

根据中朝两国政府文化合作协定,中国科学院外事局在院内组织了糖槭树考察团(四人)于1984年7月中、下旬赴朝鲜民主主义人民共和国考察,得到朝方自然保护联盟中央委员会及其有关部门、单位的热情接待和大力支持,使这次考察获得圆满成功。现仅就朝鲜木本糖料植物的研究概况报道如下:     

板栗枝插生根试验

促使枝插生根是板栗营养繁殖最有希望的方法之一,因其方法简便,同时又解决了由于板栗嫁接时常遇到的砧穗不亲和的问题。 试材为‘AU—Cropper’、‘AU—Homestead’和‘AU—Leader’三个板栗品种。插条采自植株成熟部位经修剪诱发的健壮副梢上抽生的春梢,剪取其顶部和基部枝条,长15—20厘米;此外,从植株未修剪的成熟部位,剪取当年生春梢(带有2—3厘米长的去年生枝)。插条上的叶片,仅留顶部2—3片,余者剪除,然后在近轴末端的     

Immobilization of enzymes in porous supports: Effects of support-enzyme solution contacting

Proteins have been immobilized in porous support particles held in a fixed-bed reactor through which protein solution is continuously circulated. Changing the recirculation flow rate alters the observed immobilization kinetics and the maximum enzyme loading which can be achieved for glucose oxidase and glucoamylase on carbodiimide-treated activated carbon and for glucoamylase immobilized on CNBr-Sepharose 4B. Direct microscopic examination of FITC-labelled protein in sectioned Sepharose particles and indirect activity-loading studies with activated carbon-enzyme conjugates all indicate that immobilized enzyme is increasingly localized near the outer surface of the support particles at larger recirculation flow rates. Restricted diffusion of enzymes may be implicated in this phenomenon. These contacting effects may be significant considerations in the scaleup of processes for protein impregnation in porous supports, since apparent activity and stability of the final preparation depend on internal protein distribution.     

Analysis of a continuous, aerobic, fixed-film bioreactor. I. Steady-state behavior

The analysis of a continuous, aerobic, fixed-film bioreactor is performed by simulating the behavior of penicillin production in a three-phase fluidized bed. Rigorous mathematical models are developed for a fluidized-bed fermentor in which bioparticles are fluidized by the liquid medium and air. The steady-state performance of the fluidized-bed reactor is appraised in terms of penicillin productivity and outlet concentration by considering the two extremes in contacting patterns, complete back-mix and plug flow, in the absence of a growing biofilm. The results show that the complete back-mix contacting pattern is preferred over that of plug flow due to the nature of the penicillin kinetic relationships. It is also shown that for the dual-nutrient (glucose and oxygen) penicillin reaction system the optimum biofilm thickness does not equal the penetration depth of a limiting nutrient, but depends upon the total reactor configuration.     

管花马兜铃化学成分的研究

从管花马兜铃中分得马兜铃酸-A,7-甲氧基马兜铃酸-A,马兜铃内酰胺-β-D-葡萄糖甙,尿囊素和Eupomatenoid-7。     

银杏科研的主要成就和展望

前言银杏又名白果,是现存种子植物中最古老的孑遗植物,也是源于我国的特有珍贵树种。学者们一向把它视为植物界的奇珍,在植物分类学、树木学、果树栽培学和古植物学上占有特殊的位置,至今国内外植物学家、地质学家,以及果树栽培学家们,仍对它进行着深入细致的研究。银杏的科学奥秘将逐步被揭开。     

大豆根瘤侵染细胞中一种细胞质内含物的电镜观察

在中国丰收11号大豆根瘤侵染细胞中,我们发现了一种电子密度很高,体积很大,形状为圆形或近似圆形,外面没有界膜,常位于胞间隙附近的特殊的细胞质内含物。高尔基体及其小泡,丰富的粗糙型内质网和核糖体常在它的附近,其中一些核糖体正沉积在它的表面。它主要是由核糖体凝聚而成,高尔基体和内质网在它的形成中也起了一定作用。它的内部含有颗粒状,纤维状,泡状和管状物质。它的出现似乎与侵染细胞固氮有关。     

甲型肝炎病毒对理化因子的稳定性

用放射免疫成斑法研究了甲型肝炎病毒FM-175株的理化稳定性和灭活条件,证明甲型肝炎病毒理化稳定性与其它肠道病毒相似,具有广范围的pH(2～10)稳定性;Mg~(++)和Ca可增强其热稳定性,不能抵抗冷冻干燥,但对热的抵抗力明显高于普通肠道病毒,可被紫外线迅速杀灭,也可被多种消毒剂如3～8％的甲醛液,50～90％的乙醇,2％的石炭酸及400ppm的有效氯等杀灭,但可抵抗0.1％甲醛液,2～5％的来苏儿及200ppm的有效氯1小时以上,本研究工作为甲型肝炎病毒的理化性状、保存及消毒条件等提供了实验数据。     

用PO4细胞为靶细胞检测人血清中Epstein-Barr病毒IgA/MA抗体以诊断鼻咽癌

近年来,常用未经丙酮固定的、由丁酸和巴豆油激活的B95-8细胞或P3HR-1细胞为靶细胞,检测人血清中EB病毒IgA/MA抗体以早期诊断鼻咽癌,效果良好。但由于B95-8细胞含有多种EB病毒抗原,不能用丙酮固定,需多次离心沉淀,在浮悬状态下检测,技术比较复杂。