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1.
RAPD分析─鉴定柑桔体细胞杂种的快速方法 总被引:64,自引:3,他引:61
肖顺元 Frederick G.Gmitter Jude W.Grosser 黄舒XIAO Shun-Yuan Frederick G.Gmitter Jude W.Grosser HUANG Shu 《遗传》1995,17(4):40-42
本文利用改进的DNA提取方法,从Volkamer柠檬(Citrus volkameriana Ten. and Pasq.)和酸橙(C. aurantium L.)及其原生质体杂种植株的叶片中抽提总DNA,进行RAPD(Random Amplified Polymorphic DNA)分析。结果表明: 在随机选取的15种引物中,有10种可单独或与其它引物一道鉴定这一组合的体细胞杂种。与形态学性状观察、同工酶及ONA杂交分析等方法比较,RAPD分析是一种可在试管苗期即可直接、准确、快速鉴定柑桔体细胞杂种的方法。 相似文献
2.
LI Mi LIU LianQing XI Ning WANG YueChao DONG ZaiLi XIAO XiuBin ZHANG WeiJing 《中国科学:生命科学英文版》2013,56(9):811-817
Atomic force microscopy (AFM) was used to examine the morphology of live mammalian adherent and suspended cells. Time-lapse AFM was used to record the locomotion dynamics of MCF-7 and Neuro-2a cells. When a MCF-7 cell retracted, many small sawtooth-like filopodia formed and reorganized, and the thickness of cellular lamellipodium increased as the retraction progressed. In elongated Neuro-2a cells, the cytoskeleton reorganized from an irregular to a parallel, linear morphology. Suspended mammalian cells were immobilized by method combining polydimethylsiloxane-fabricated wells with poly-L-lysine electrostatic adsorption. In this way, the morphology of a single live lymphoma cell was imaged by AFM. The experimental results can improve our understanding of cell locomotion and may lead to improved immobilization strategies. 相似文献
3.
YANG Lei RONG WeiQi XIAO Ting ZHANG Ying XU Bin LIU Yu WANG LiMing WU Fan QI Jun ZHAO XiuYing WANG HongXia HAN NaiJun GUO SuPing WU JianXiong GAO YanNing CHENG ShuJun 《中国科学:生命科学英文版》2013,56(7):638-646
For successful therapy, hepatocellular carcinoma (HCC) must be detected at an early stage. Herein, we used a proteomic approach to analyze the secretory/releasing proteome of HCC tissues to identify plasma biomarkers. Serum-free conditioned media (CM) were collected from primary cultures of cancerous tissues and surrounding noncancerous tissues. Proteomic analysis of the CM proteins permitted the identification of 1365 proteins. The enriched molecular functions and biological processes of the CM proteins, such as hydrolase activity and catabolic processes, were consistent with the liver being the most important metabolic organ. Moreover, 19% of the proteins were characterized as extracellular or membrane-bound. For validation, secretory proteins involved in transforming growth factor-β signaling pathways were validated in plasma samples. Alphafetoprotein (AFP), metalloproteinase (MMP)1, osteopontin (OPN), and pregnancy-specific beta-1-glycoprotein (PSG)9 were significantly increased in HCC patients. The overall performance of MMP1 and OPN in the diagnosis of HCC remained greater than that of AFP. In addition, this study represents the first report of MMP1 as a biomarker with a higher sensitivity and specificity than AFP. Thus, this study provides a valuable resource of the HCC secretome with the potential to investigate serological biomarkers. MMP1 and OPN could be used as novel biomarkers for the early detection of HCC and to improve the sensitivity of biomarkers compared with AFP. 相似文献
4.
WEI MIN NI XIAO YA CHEN ZHI HONG XU HONG WEI XUE. * National Laboratory of Plant Molecular Genetics Institute of Plant Physiology Ecology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Partner Group of Max-Pla 《Cell research》2002,(Z1)
Polar auxin transport plays a divergent role in plant growth and developmental processes including root and embryo development, vascular pattern formation and cell elongation. Recently isolated Arabidopsis pin gene family was believed to encode a component of auxin efflux carrier (G(?)lweiler et al, 1998). Based on the Arabidopsis pin1 sequence we have isolated a Brassica juncea cDNA (designated Bjpin1), which encoded a 70-kDa putative auxin efflux carrier. Deduced BjPIN1 shared 65% identities at protein level with AtPINl and was highly homologous to other putative PIN proteins of Arabidopsis (with highest homology to AtPIN3). Hydrophobic analysis showed similar structures between BjPINl and AtPIN proteins. Presence of 6 exons (varying in size between 65 bp and 1229 bp) and 5 introns (sizes between 89 bp and 463 bp) in the genomic fragment was revealed by comparing the genomic and cDNA sequences. Northern blot analysis indicated that Bjpin1 was expressed in most of the tissues tested, with a relatively h 相似文献
5.
Wen-rong YAO Guo-qiang PAN Cheng-long XIONG Qian-fu ZHOU Qi-you XIAO Ming-hui LI Yong-zhen ZHANG 《Virologica Sinica》2007,22(4):307-315
Saliva and blood were collected from two patients who had not received post exposure prophylaxis in the cities of Wenzhou and Xinning respectively. Both patients were confirmed as positive for rabies by detection of rabies virus specific nucleoprotein antibodies in the sera by Western Blot. However, rabies virus specific RNA was only identified in the saliva collected from the patient in Wenzhou. Furthermore, the isolate Zhejiang Wz0 (H) was obtained by inoculating one-day-old suckling mice. Both nucleoprotein (N) and glycoprotein (G) genes from the isolate were amplified by RT-PCR and sequenced. Phylogenetic analysis indicated that the isolate belonged to classic rabies virus, and shared a higher homology with the street viruses from dogs in the main endemic areas in China and the street virus from dogs in Indonesia than with other known strains. Further comparison of the deduced amino acid sequences between the isolate and the vaccine strains used in China showed that the virus had a higher level of homology with the vaccine strain CTN than with the other vaccine strains (3aG, PV, PM and ERA). In particular, amino acid residues substitutions located in antigenic site Ⅲ in the G protein, which could react with the neutralizing antibodies, were observed. These results suggested that the virus belonged to the classic rabies virus, and both N and G genes diverged from the current vaccine strains used in China at either the nucleotide or the amino acid level. 相似文献
6.
The complete mitochondrial genomes sequences of Asio flammeus and Asio otus and comparative analysis 总被引:2,自引:0,他引:2
SUN Yi MA Fei XIAO Bing ZHENG Junjie YUAN Xiaodong TANG Minqian WANG Li YU Yefei & Li Qingwei . College of Life Sciences Liaoning Normal University Dalian China . TaKaRa Biotechnology 《中国科学:生命科学英文版》2004,47(6):510-520
Mitochondrial DNA (mtDNA), as a model sys-tem, has been extensively used for molecular phy-logenetic and evolutionary analysis[1]. With the ad-vances in DNA sequencing technology, more andmore researchers prefer to use complete mitochondrialgenome for phylogenetic analysis[2—4]. Since the com-plete sequencing of human mtDNA in 1981 (Andersonet al., 1981)[5], 342 vertebrate mitochondrial genomeshave so far been sequenced. Up to now the completesequences of 29 avian mitochondrial genomes h… 相似文献
7.
为克隆精子发生相关基因的全长cDNA,根据mRNA差异显示获得的ESTs设计引物,利用一种新的cDNA末端快速扩增方法(SMARTRACE)扩增该EST的5′末端,并进行克隆测序,与mRNA差异显示获得ESTs拼接后,获得了三个新的全长cDNA.结果表明,SMARTRACE是一种简便、有效的克隆cDNA5′末端未知序列的技术.
Abstract:To clone the full-length cDNAs of genes related to spermatogenesis,ESTs obtained by mRNA differential display were used to design gene-specific primer.Then SMART RACE was performed to obtain the 5′ region of these ESTs.After cloning,sequencing and splicing with ESTs obtained by mRNA differential display,three full-length cDNAs were obtained.The results indicate that SMART RACE is a simple and an effective technique for cloning 5′-end unknown sequence of gene. 相似文献
8.
Li-Qing XIAO Ai-Hua LIU and Yong-Lian ZHANG State Key Laboratory of Molecular Biology Institute of Biochemistry Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Graduate School of the Chinese Academy of Sciences Shanghai China 《Acta biochimica et biophysica Sinica》2004,(8)
Defensins are a family of evolutionarily relatedvertebrate antimicrobial peptides with a characteristicβ-sheet-rich fold and a framework of six disulphide-linkedcysteines. The two main defensin subfamilies, α- andβ-defensin, differ in the length of pep… 相似文献
9.
家禽(鹅、鸭、鸡)血清酯酶多态性比较血型学初步研究 总被引:9,自引:2,他引:7
鸡的酯酶在两个区域出现了变异, 靠近阳极的Es-1区共有3条带,表现出7种表型组合(AA、 BB、CC、AB、AC、BC和无带O型),分别受控于常染色体上相同基因座位上3个复等位基因(Es-1^A|、Es-1^B|、Es-1^C|)和1个隐性基因(Es-1^O|)。在原点和Es-1之间的Es-2区表现出有带(+)和无带(-)两种类型。鹅的血清酯酶与鸡截然不同。在鸡的Es-2区域,鹅的带谱信号弱(无)。在与鸡Es-1区域相应位置,鹅存在着8-9条谱带,其中的1号、2 -7号存在着个体水平的多态现象。鸭血清酯酶的聚丙烯酰胺谱带与鹅非常相似。鹅、鸭血清酯酶的遗传机制有待交配实验确定。 相似文献
10.
CHEN Ruijuan JIANG Jiangang XIAO Xiao & WANG Daowen . The Institute of Hypertension Department of Internal Medicine Tongji Hospital Tongji Medical College Huazhong University of Science & Technology Wuhan China . Departments of Molecular Genetics Biochemistry & Gene Therapy Center University of Pittsburgh Pittsburgh PA USA 《中国科学:生命科学英文版》2005,48(5):495-505
Vascular endothelial cells play crucial roles in regulating cardiovascular function, maintaining car-diovascular homeostasis and preventing the occur-rence of cardiac and cerebral vascular diseases. All these protective effects are fulfilled through various vasoactive products secreted by endothelium including nitric oxide (NO), prostacyclin (PGI2) and endothe-lium-derived hyperpolarizing factor (EDHF). NO, pro-duced from L-arginine by endothelial nitric-oxide synthase (eNOS), is an impor… 相似文献