多通道流动电泳技术及其在蛋白质、酶和抗体纯化中的应用研究

提出一种新型制备电泳方法即多通道流动电动电泳技术,建立了微机控制的电泳设备,考查了操作条件对设备分离效率的影响。应用该技术进行牛血清清蛋白(BSA)-牛血红蛋白(HBB)混合物的连续分离,每小时从含BSA、HBB各37.0mg的蛋白质混合物中分离出13.6mg的BSA和20.0mg的HBB。应用该技术进行尿激酶粗品的纯化,可将其比活力由4000IU/mg提高到4×10~4IU/mg以上,产量在10×10~4IU/h以上。将该技术与(NH_4)SO_4沉淀方法结合,从15ml免疫鼠血清中分离出12.5mg高纯度尿激酶抗体IgG,产量为2.1mg/h。上述结果证明多通道流动电泳技术具有分离速度快、精度高和可操作性好等优点,在生物产品分离领域中具有广阔的应用前景。     

Influence of silicon on microdistribution of mineral ions in roots of salt-stressed barley as associated with salt tolerance in plants

Two contrasting barley (Hordeum vulgare L.) cultivars: Kepin No.7 (salt sensitive), and Jian 4 (salt tolerant) were grown hydroponically to investigate the microdistribution of mineral ions in roots as affected by silicon (Si) with respect to salt tolerance. The experiment was undertaken consisting of two treatments with 3 replicates: (i) 120 mmol · L⁻¹ NaCl alone (referred to as Si-NaCl+), (ii) 120 mmol · L⁻¹ NaCl + 1.0 mmol · L⁻¹ Si (as potassium silicate) (referred to as Si+NaCl+). Plant root tips were harvested for microanalysis using an energy dispersive X-ray microanalyzer (EDX) 30 d after transplanting. Higher Cl and Na X-ray peaks were recorded in the root epidermal, cortical and stelar cells of roots for the treatment Si-NaCl+ with the majorities of Na and Cl being accumulated in epidermal and cortical cells, while relatively low K peaks were observed regardless of the barley cultivars used. By contrast, considerably higher K peaks were detected in the epidermal, cortical and stelar cells of the roots for the treatment Si+NaCl+, but lower Cl and Na peaks were also observed for this treatment with both Na and Cl ions being evenly distributed in the epidermal, cortical and stelar cells. These findings directly support our previous finding, which showed that Si depressed the uptake of sodium but enhanced the uptake of potassium by salt-stressed barley. We believe that one of the possible mechanisms involved in Si-enhancement of salt tolerance in barley is attributed to the Si-induced changes in the uptake and microdistribution of mineral ions in plants.     

延髓腹外侧区在降压反射中的作用

在各种心血管反射中,降压反射是最主要的,延髓腹外侧区在降压反射中起重要作用。目前认为降压反射中枢通路中至少有四种成分是最基本的：（１）孤束核中的神经元;（２）延髓腹外侧头端的交感前运动细胞;（３）延髓腹外侧尾端;（４）疑核或和迷走神经背运动核。此外,兴奋性与抑制性氨基酸受体和抑制性神经元也是中枢通路的关键成分。下丘脑视上核与室旁核的升压素分泌细胞也有一定作用     

银耳袋栽高产优质技术

银耳,学名ＴｒｅｍｅｌａｆｕｃｉｆｏｒｍｉｓＢｅｒｋ。别名白木耳、雪耳、川耳,属于层菌纲、异隔担子亚纲、银耳目、银耳属。银耳与其他菌类不同,它的菌丝体是一个混合菌丝结构,即由银耳纯菌丝和一种称为“耳友菌”或称“伴生菌”的香灰菌丝（亦称羽毛状菌丝）构成...     

百脉根的综合利用

百脉根的综合利用丁玉川俞小秋百脉根（Ｌｏｔｕｓｃｏｒｎｉｃｕｌａｔｕｓ）,又名牛角花、五叶草,蝶形花科多年生草本植物。高１０—６０厘米。小叶三出,生于叶柄基部的２枚托叶与３小叶相似,故欲称“五叶草”。花冠黄色,长１厘米以上。荚果长圆柱形。种子绿色,肾...     

中国厉螨科二新种记述(蜱螨亚纲:革螨股)

记述厉螨科Ｌｅａｌａｐｉｄａｅ Ｂｅｒｌｅｓｅ,１８９２二新种：拟巴阳厉螨Ａｎｄｒｏｌａｅｌａｐｓ ｓｕｂｐａｖｌｏｖｓｋｉｉ Ｌｉｕ,Ｍａ ｅｔ Ｄｉｎｇ,ｓｐ．ｎｏｖ．和峡江广厉螨Ｃｏｓｍｏｌａｅｌａｐｓ ｘｉａｊｉａｎｇｅｎｓｉｓＬｉｕ ｅｔ Ｍａ,ｓｐ．ｎｏｖ．,文中详细描述了新种形态特征,并与相近种作比较。     

乙脑病毒全长cDNA的扩增克隆及体外转录制备感染性RNA的研究

本研究根据已知的乙脑病毒(JEV)SA14株基因序列设计一套引物 ,利用长模板RT-PCR 技术,一次扩增出了JEV的全长cDNA,并采用大片段克隆技术将其克隆于载体的RNA聚合酶启动子下游.阳性克隆转录的RNA转染HBK细胞后细胞产生病变,经乳鼠脑内接种及特异性免疫荧光染色证明为JEV,这一结果表明JEV感染性克隆的构建获得了成功.其次,合成一条含有 RNA聚合酶启动子序列的5′引物,利用长模板RT-PCR方法扩增出带有启动子的JEV全长cDNA ,以此cDNA为模板作体外转录.转录产物转染BHK细胞后观察到了典型的JEV病变.收获的病毒经乳鼠脑内接种及免疫荧光染色证明为JEV,从而建立了制备JEV感染性RNA的快捷方法.本研究构建的JEV感染性克隆与建立的长模板RT-PCR快速制备JEV感染性RNA的方法,将为JEV 分子生物学研究的后续工作提供有力的工具和奠定坚实的基础.     

土施L-蛋氨酸、L-苯基丙氨酸、L-色氨酸对玉米生长和养分吸收的影响

通过盆栽试验,研究了3种植物生长调节剂前体物质对玉米生长发育和养分吸收的影响,并确定了其适宜用量.结果表明,土施L蛋氨酸(L-MET)、L-苯基丙氨酸(L-PHE)和L-色氨酸(L-TRP)能不同程度地增加玉米株高、地上部和地下部干重,提高玉米根系活力、体内硝酸还原酶和过氧化氢酶的活性,促进玉米对氮、磷、钾、锌养分的吸收.在所有供试浓度中,以土施L-MET0.0185～0.185mg·kg^-1、L-PHE0.2mg·kg^-1和L-TRP0.03～0.3mg·kg^-1效果最佳,而且L-PHE和L-TRP对玉米生长的促进作用和提高养分的吸收能力均优于L-MET.     

葛根淀粉的采集和加工方法

葛,又名野葛、葛藤,豆科藤本。块根含淀粉20％以上。经过粉碎、漂洗、沉淀等工序,可以从葛中分离提取出葛粉,其质地洁白、细嫩,除富含大量淀粉外,还含少量维生素和矿物质,是营养丰富的高级淀粉。将葛粉用沸水冲调,即成为稠粘胶状,晶莹透明、气味芳香、爽滑可口,加入食糖或其他佐料搅拌后,就成为一种美味的凉点,此外还可制糕饼、粉丝等。其产品易于硝化,还具有清热润肺的功效。十分适于老人、小孩、产妇和病人食