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1.
Characterization of B and H blood-group active glycosphingolipids from human B erythrocyte membranes
Peter Hanfland 《Chemistry and physics of lipids》1975,15(2):105-124
Two blood group B active glycosphingolipids (B-I and B-II) previously isolated and highly purified from human B erythrocytes [21] were analysed first by degradation with α-D-galactosidase from coffee beans, α-L-fucosidase from bovine kidney and with 0,1 N trichloracetic acid; the native B-glycolipids as well as their degradation products were then investigated by methylation analysis with combined gas chromatography-mass spectrometry, by thin layer chromatography, twodimensional immunodiffusion and by the hemagglutination inhibition technique. Together with the results obtained by mass spectrometry of permethylated glycolipids [26] the following structures were elucidated: α-D-galactopyranosyl-(1 → 3)-[α-L-fucopyranosyl-(1 → 2)]-D-galactopyranosyl-(1 → 4)-N-acetyl-D-glucosaminosyl-(1 → 3)-D-galactopyranosyl-(1 → 4)-D-glucopyranosyl-(1 → 1)-ceramide for the B-I glycosphingolipid and α-D-galactopyranosyl-(1 → 3)-[α-L-fucopyranosyl-(1 → 2)]-D-galactopyranosyl-(1 → 4)-N-acetyl-D-glucosaminosyl-(1 → 3)-D-galactopyranosyl-(1 → 4)-N-acetyl-D-glucosaminosyl-(1 → 3)-D-galactopyranosyl-(1 → 4)-D-glucopyranosyl-(1 → 1)-ceramide for the B-II glycosphingolipid. A H active glycolipid fraction from B erythrocytes further purified by thin layer chromatography was also investigated by methylation analysis. The pattern of its partially methylated alditol acetates was essentially the same as that of the α-galactosidase treated and permethylated B-I glycolipid. It also exhibited strongly precipitating and hemagglutination inhibiting H properties as well as the two α-galactosidase treated B-I and B-II glycosphingolipids. Based upon these data the following tentative structure was proposed: α-L-fucopyranosyl-(1 → 2)-D-galactopyranosyl-(1 → 4)-N-acetyl-D-glucosaminosyl-(1 → 3)-D-galactopyranosyl-(1 → 4)-D-glucopyranosyl-(1 → 1)-ceramide. Gas chromatographic analysis revealed sphingosine and lignoceric, nervonic and behenic acids to be the main components of the ceramide residues of the three glycosphingolipids. From the data presented the H active substance very probably can be regarded as the immediate precursor of the B-I glycosphingolipid from human B erythrocyte membranes. 相似文献
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利用 XAD-4憎水性吸附树脂采集墨红头香,以毛细管气相色谱双柱保留指数和 GC/MS/DS 联用方法鉴定头香的化学成份。共分离鉴定或初步鉴定了45种组份,其中含量较大的有乙酸芳樟酯(14.98%),柠檬烯(12.07%),甲基苯甲醚(9.88%),香茅醇(4.82%),乙酸巳酯(3.98%),β-石竹烯(4.55%),芳樟醇(3.18%),正巳醇(3.17%)等. 相似文献
4.
Two population groups of Jammu and Kashmir (India) — Muslims and Bodhs-have been typed for haptoglobin and for CG and PI subtype
polymorphisms. The allele frequencies do not show significant differences between these two populations. HP and GC allele
frequencies of Bodhs and Muslims differ considerably from with that observed in other North Indian populations. The PI allele
frequencies of Bohds and Muslims differ considerably from those found in other Indian populations and are more similar to
Mongoloid ones. 相似文献
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“同时蒸馏-萃取”分析茉莉花香成分 总被引:5,自引:0,他引:5
用一种经过改进的“同时蒸馏-萃取”仪提取了茉莉花的香成分,以 GC/MS 和 Kovats保留指数法鉴定了提取物中的28个化学成分。主要成分为:芳樟醇、乙酸苯甲酯、顺-石竹萜烯、榧烯醇、苯甲酸顺-3-己烯酯、邻氨基苯甲酸甲酯、二十三烯-11及吲哚。讨论了鲜花香成分和植物精油的采集与分析方法。 相似文献
7.
本文报道了从腐梨上分离到的一株未见描述的毕赤酵母属新种(Pichia hangzhou-ana Lu et Li),并讨论了与其近似种的区别。同时进行了DNA G+C百分比值的测定。 相似文献
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内源植物激素与光敏核不育水稻农垦58S育性的关系 总被引:22,自引:1,他引:21
采用气相色谱-质谱联用选择离子检测(GC-MS-SIM)技术,定性、定量分析了光敏核不育水稻农垦58S(Oryza sativa L. subsp. japonica)及对照品种“农垦58”在长(LD)、短(SD)日照处理下,不同发育时期顶端全展叶片中内源IAA、ABA 和生殖器官中内源IAA、ABA、GA1、GA4 的含量变化。实验结果表明:在LD 处理下,农垦58S雌雄蕊形成期的叶片和花粉母细胞形成期的幼穗中IAA 相继发生亏缺;农垦58S-LD花粉母细胞形成期、单核期和扬花期,生殖器官中内源ABA 均低于农垦58S-SD 的含量,“农垦58”则与此相反;扬花期不育花药中内源GA1 和GA4 含量剧减。据此认为:生殖器官中早期IAA 的亏损、ABA 含量剧减伴随着抗逆性能的减弱及GAS的不足共同导致了农垦58S的雄性败育 相似文献
10.
The application of cellulose-based stationary phases for chiral separations has been extended to open tubular column chromatography. Efficient columns were obtained by coating the capillaries with mixtures of chiral cellulose materials and conventional achiral stationary phases for gas chromatography. In this study, various siloxane and polyethylene glycol polymers were used as achiral components and mixed with different substituted benzoylcellulose derivatives as chiral components. Systematic investigations were carried out to determine the optimal ratio for the components of the stationary phase. Depending on the chromatographic mode—gas chromatography (GC) or supercritical fluid chromatography (SFC)—the stationary phases were found to behave differently. The applicability of the technique was demonstrated by the resolution of various racemic compounds. © 1993 Wiley-Liss, Inc. 相似文献