藏区金腰种质资源调查与分析

金腰属植物因富含黄酮醇类化合物而具有显著的药效,通过对我国西藏、云南、四川、青海以及甘肃5个省(自治区)进行金腰属植物种质资源的科学考察,共收集到19种(含2变种)145份野生种质资源。此外四川省发现金腰新分布2种,分别为秦岭金腰(Chrysosplenium biondianum Engl.)、陕甘金腰(Chrysosplenium qinlingense Z.P.Jien&J.T.Pan),均为中国特有种;甘肃省发现金腰新分布3种,分别为毛金腰(Chrysosplenium pilosum Maxim.)、绵毛金腰(Chrysosplenium lanuginosum Hook.f.&Thomson)和蜕叶金腰(Chrysosplenium henryi Franch.),凭证标本存于中南民族大学植物标本馆(HSN)。金腰属植物作为传统藏药长期以来被用于治疗各种疾病,如长梗金腰(Chrysosplenium axillare Maxim.)的75%乙醇提取物具抗炎症、抗流感病毒活性,从灰花金腰(Chrysosplenium grayanum Maxim.)与裸茎金腰(Chrysosplenium nudicaule Bunge)提取的五环三萜对肿瘤细胞有较强抑制效果。本调查丰富了野生藏药金腰属植物种质资源,为深入研究金腰属植物的分类与药理活性提供了基础。     

蝴蝶果茎挥发油的化学成分

采用水蒸汽蒸馏法从大戟科蝴蝶果茎中提取挥发油,用气相色谱-质谱联用技术对挥发油化学成分进行分析。分离出36个峰,鉴定出35种化合物,占总油量的98.34%,并应用面积归一化法测定各成分的相对百分含量。其主要成分为十六烷酸乙酯(13.19%)、正十六烷酸(11.11%)、十八碳烯酸乙酯(6.18%)、正十八烷(4.98%)、(Z,Z)-9,12-十八碳二烯酸(4.90%)及十八碳二烯酸乙酯(4.21%)。     

基于RAPD数据探讨中华蓑藓(Macromitrium cavaleriei Card. & Thér.)形态变异的遗传基础

中华蓑藓（Macromitrium cavaleriei Card.＆Thér.）的形态变异强烈,为了解形态变异是否具有遗传学背景,本研究以采自浙南凤阳山、浙中金华北山、浙西北的天目山和江西庐山4个种群的中华蓑藓为材料,运用随机扩增多态性DNA（RAPD）探讨了中华蓑藓的遗传多样性。从100条随机引物中筛选出了15条有效引物,利用它们共获得183个条带,其中多态性条带占79·69%,中华蓑藓各种群间的Dice遗传距离为0·0732~0·1514。POPGENE32软件分析得到种的Nei基因多样性指数（H）为0·3378,Shannon指数（I）为0·5126,遗传分化系数（Gst）为0·1670;基因流（Nm）为2·4947;种群内遗传多样性指数为0·4055,占种群总的遗传多样性的79·11%,反映出研究区域内的中华蓑藓遗传变异大多数存在于种群内,中华蓑藓形态变异并没有多少遗传背景,很可能是生态环境因素引起的可塑性变异。聚类分析表明,中华蓑藓种群遗传距离与地理距离之间无直接相关关系,遗传多样性水平与物种特性和所处不同群落有关。虽然4个种群内的形态变异丰富,但是属于同一物种的范围。     

贵州鼠尾草化学成分的研究

从贵州鼠尾草95%乙醇提取物分离得到8个化合物,通过理化性质及波谱方法分别鉴定为正三十三烷(1),十二烷酸十四烷酯(2),β-谷甾醇(3),胡萝卜苷(4),乌苏酸(5),2 a,3β-二羟基乌苏酸(6),白桦酸(7)和迷迭香酸(8)。以上化合物均首次从该植物中分离得到。     

A Study on the Genus Chrysosplenium L. from China (Cont.)

This paper deals with the taxonomy and geographic distribution of the genus Chrysosplenium L. in China. Based on the characters and evolution of the seed, capsule, disk, ovary and leaf, the species of this genus can be grouped into 2 subgenera, 5 sections and 16 series. There are 2 subgenera, 5 sections and 11 series in China. They are as follows: I. Subgen. Gamosplenium Maxim. emend. J. T. Pan Leaves alternate. Lectotype: Chrysosplenium carnosum Hook. f. et Thoms. 1. Sect. Alternifolia Franch. emend. J. T. Pan Seeds smooth and glabrous. Type: Chrysosplenium alternifolium L. (1) Ser. Nudicaulia Maxim. emend. J. T. Pan Disk obscure or absent; ovary nearly half-inferior, sometimes mostly inferior; capsule generally subtruncate and emarginate at top and bilobed with equal and horizontally divaricate or suberect lobes; seeds smooth and glabrous. Type: Chrysosplenium nudicaule Maxim. (2) Ser. Alternifolia Maxim. emend. J. T. Pan Disk 8-lobed; ovary nearly half-inferior; capsule generally subtruncate and emarginate at top, and bilobed with equal and horizontally divaricate lobes; seeds smooth and glabrous. Type: Chrysosplenium alternifolia L. 2. Sect. Nephrophylloides Turcz. Seeds minutely papillose or pilose. Type: Chrysosplenium sedakowii Turcz. (1) Ser. Macrophylla Franch. emend. J. T. Pan Disk obscure or absent; ovary nearly half-inferior; capsule nearly truncate and emarginate at top, and bilobed with equal lobes; seeds minutely papillose. Type: Chrysosplenium macrophyllum Oliv. (2) Ser. Ovalifolia Maxim. emend. J. T. Pan Disk generally 8-, rarely 4-, lobed, papillae absent around disk; ovary mostly inferior; capsule subtruncate and emarginate at top; seeds minutely papillose or pilose. Type: Chrysosplenium ovalifolium M. Bieb. ex Bunge (3) Ser. Lanuginosa Hara, emend. J. T. Pan Papillae numerous, brown around reduced disk; ovary mostly inferior; capsule nearly truncate and emarginate at top; seeds minutely papillose. Type: Chrysosplenium lanuginosum Hook. f. et Thoms. II. Subgen. Chrysosplenium Leaves opposite. Type: Chrysosplenium oppositifolium L. 1. Sect. Trichosperma J. T. Pan, sect. nov. Capsule not truncate at top, and bilobed with subequal, suberect or divergent lobes. Type: Chrysosplenium trichospermum Edgew. ex Hook. f. et Thoms. This section is divided into 4 series in the world, with only 1 in China. (1) Ser. Nepalensia Maxim. emend. J. T. Pan Disk obscure or absent; ovary generally mostly inferior; cassule not truncate at top, and bilobed with subequal and suberect or divergent lobes; seeds smooth and glabrous. Type: Chrysosplenium nepalense D. Don 2. Sect. Grayana J. T. Pan, sect. nov. Capsule bilobed with distinctly unequal and ascending lobes. Type: Chrysosplenium grayanum Maxim. This section consists of 4 series in the world, with 3 series in China. (1) Ser. Sinica Maxim. emend. J. T. Pan Disk obscure or absent; ovary nearly half-superior; capsule bilobed with distinctly unequal and ascending lobes; seeds minutely papillose. Type: Chrysosplenium sinicum Maxim. (2) Ser. Esulcata Franch. emend. J. T. Pan Disk (4)-8-lobed; ovary generally half-inferior; capsule bilobed with unequal and ascending lobes; seeds minutely papillose or pilose. Lectotype: Chrysosplenium dubium J. Gayex DC. (3) Ser. pilosa maxim. emend. J. T. Pan Disk obscure or absent; ovary nearly half-inferior; capsule bilobed with distinctly unequal and ascending lobes; seeds distinctly longitudinally ll-18-costate and minutely papillose or tuberculate on the ridge. Type: Chrysosplenium pilosum Maxim. 3. Sect. Chrysosplenium Capsule nearly truncate and emarginate at top, and bilobed with equal and horizontally divaricate lobes. Type: Chrysosplenium oppositifolium L. (1) Ser. Romosa J. T. Pan, ser. nov. Disk distinctly 8-lobed, papillae sparse, brown around disk; ovary mostly inferior; capsule nearly truncate and emarginate at top, and bilobed with equal and horizontally divaricate lobes; seeds smooth and glabrous. Type: Chrysosplenium ramosum Maxim. This series is monospecific one, also occurring in China, namely C. ramosum Maxim. (2) Ser. Delavayi Hara Disk distinctly 8-lobed, Papillae sparse, brown around the disk; ovary mostly inferior; capsule nearly truncate and emarginate at top, and bilobed with equal and horizontally divaricate lobes; seeds distinctly longitudinally 10-16-costate and transversely striate on the ridge. Type: Chrysosplenium delavayi Franch. This series can be considered as the most advanced one in the Chrysaspleninm L. So far, the Chrysosplenium L. comprises 64 species in the world, among which 1 species is found in North Africa, 2 in South America, 4 in Europe, 5 in North America, 56 in Asia, of which 3 occur in Sikkim, 5 Bhutan, 5 Mongolia, 6 north Burma, 6 Korea, 7 north India, 8 Nepal, 12 Japan, 17 U.S.S.R. (of which 3 also in Europe), 34 China (including 22 endemic species and 3 new species). In China, Fujian and Guangdong Provinces and Zhuang Autonomous Region of Guangxi each has only 1 species, Taiwan, Zhejiang, Shanxi and Hebei Provinces and Uygur Autonomous Region of Xinjiang each has 2, Anhui, Jiangxi and Hunan Provinces each has 3, Qinghai Province 4, Heilongjiang, Liaoning and Guizhou Provinces each has 5, Jilin and Hubei Provinces each has 6, Gausu Province 8, Shaanxi Province and Xizang (Tibet) Autonomous Region each has 10, Yunnan Province has 11, Sichuan Province has 14. Thus the distribution centre of this genus should be in the north temperate zone of Asia, and the region covering Shaanxi Gansu, Sichuan, Yunnan and Xizang may be regarded as an important part of this centre. The 7 species of Ser. Nudicaula Maxim. emend. J. T. Pan can be considered as the most primitive ones in this genus. They are mostly distributed in Shaanxi (Qin Ling), south Gansu, southeast Qinghai, southwest Sichuan and nothwest Yunnan of China. This region may be considered as the centre of the origin (or at least differentiation) of this genus. The new species and the new varieties described in this paper are as follows: C. hydrocotylifolium Levl. et Vant. var. emeiense J. T. Pan, C. taibaishanense J. T. Pan, C. lixianense Jien ex J. T. Pan, C. qinlingense Jien ex J. T. Pan.     

蝴蝶果茎枝化学成分的研究

从蝴蝶果(Cleidiocarpon cavalerie)茎枝的乙醇提取物中提取并鉴定了7个化合物,利用现代波谱技术(MS、1H-NMR、13C-NMR)进行结构鉴定,结果表明,它们分别为kauran-16-ol-3-one(1)、16β,17-dihydroxy-3-oxo-phyllocladane(2)、kauranoic acid(3)、山萘酚(4)、没食子酸(5)、β-谷甾醇(6)和胡萝卜苷(7)。化合物1~7均为首次从该属植物中获得。     

蝴蝶果根、果仁挥发油化学成分的研究

采用水蒸气蒸馏法从大戟科蝴蝶果的根和果仁中提取挥发油,用气相色谱-质谱联用技术对挥发油化学成分进行分析,并应用面积归一化法测定各成分的相对百分含量。从根中鉴定出23种化合物,占总油量的100%,其主要成分为十六烷酸(18.74%)、(Z,Z)9,12-十八碳二烯酸(12.81%)、1,2-苯二羧酸双(2-甲基丙基)酯(10.59%)、二丁基邻苯二甲酸酯(7.42%)及二十四烷(6.72%)。从果仁中鉴定出17种化合物,占总油量的100%,主要成分为(E-)-9-十八碳烯酸(23.15%)、正十六烷酸(21.20%)、(Z,Z)-9,12-十八碳二烯酸(19.26%)及3-甲基十七烷(6.80%)。     

安徽金腰属 (虎耳草科)植物新资料

楔叶金腰为金腰属一新变种,同时报道安徽新记录种肾萼金腰,并对安徽金腰属植物进行了补充记述。     

Refugia within refugia: the case study of a canopy tree (Eurycorymbus cavaleriei) in subtropical China

Aim Eurycorymbus cavaleriei (Lévl.) Rehd. et Hand.‐Mazz. (Sapindaceae) is a Tertiary relict tree endemic to subtropical China. This area is a centre for speciation and evolution within the East Asia biome and one of its most important refugial locations. In this study we aim to elucidate the phylogeographical patterning in E. cavaleriei, in order to identify the locations of the species’ main refugia and the predominant patterns of migration that have led to the contemporary spatial genetic structure of chloroplast variation. Location Subtropical China. Methods We sampled 18 populations of E. cavaleriei throughout its geographical range. Chloroplast DNA (cpDNA) sequence data from two non‐coding regions ((trnS/trnG and pl20/5′‐rps12) were obtained from 170 individuals for phylogeographical analyses. Relationships among cpDNA haplotypes were determined using median‐joining networks. Genetic structure was examined by spatial analysis of molecular variance (SAMOVA). Population differentiation was estimated by G_ST and N_ST statistics. Results Ten distinct haplotypes were identified. The level of differentiation among populations was relatively high (G_ST = 0.817), and N_ST was significantly higher than G_ST (P < 0.05), indicating that strong phylogeographical structure is exhibited by this species. The SAMOVA revealed five diverging groups of related haplotypes, which coincide with major landscape features in this region. Main conclusions The high differentiation among populations of E. cavaleriei may be a combined effect of historical and contemporary processes, such as the low effective population size for the chloroplast genome of a dioecious species, long‐term range fragmentation and limited seed dispersal for the species. Clear‐cut geographical distributions of ancestral haplotypes of the species suggest multiple potential refugia across subtropical China. The identified refugial regions have long been recognized as centres of plant diversity and endemism for China and have also been suggested as glacial refugia for many other plant species. The combination of these factors means that these locations should be considered as the highest priority for inclusion in conservation policies and sustainable forest management strategies for subtropical China.     

金腰属植物化学成分和构效关系研究进展

介绍从金腰属(Chrysospleruium)植物中分离得到的化合物及其生理活性研究概况和构效关系,为进一步开展该属植物成分和药效研究提供参考。金腰属植物成分主要为黄酮类化合物,还有五环三萜。都有较强的生物活性。