ENZYMIC ACTIVITIES IN DISSOCIATED NEURONS DIFFERENTIATED IN CULTURES IN VITRO

Abstract— The appearance of the enzymes succinate dehydrogenase, lactate dehydrogenase, acid phosphatase, monoamine oxidase and acetylcholinesterase in cultures of dissociated spinal cord, dorsal root ganglia and cerebellum removed at different stages of development have been studied. The neurons differentiated in vitro have morphological and cytochemical characteristics similar to those of the normally developing neurons. On the basis of the results obtained, it is argued that in this experimental model the histogenetic determination of the neuroblasts at the time of the explant may start and bring to an end the morphological and biochemical differentiation of the various types of neurons even under conditions extremely different from those of normal development.     

中国蜱螨学研究进展概况

<正> 我国蜱螨学的研究起步较晚,解放前只有一些零星报道,比较系统的研究是从50年代开始。1963年在长春召开了第一届全国蜱螨学术讨论会,至今已有30年。近十几年来,蜱螨学的研究进展较快,中国经济昆虫志中有关蜱     

TILLING在水稻育种中的应用前景

TILLING(Targeting Induced Local Lesions in Genomes)是功能基因组研究中应用的一种反向遗传学技术。它能高通量低成本地在EMS诱变群体中鉴定出发生在特定基因上的点突变。在其基础上发展出的EcoTILLING技术则可发现种质资源中的SNP位点及小插入或缺失多态性位点。水稻是非常重要的粮食作物, 也是已经完成了全基因组序列测定,有丰富的生物信息学资源可以利用的基因组研究模式植物。水稻的分子标记辅助育种将在育种中扮演越来越重要的角色。在这样的背景下,本文从基于特定基因的种质资源鉴定、EMS诱变育种、及水稻功能标记开发等方面论述了其在水稻育种中的应用前景。