TILLING在水稻育种中的应用前景

TILLING(Targeting Induced Local Lesions in Genomes)是功能基因组研究中应用的一种反向遗传学技术。它能高通量低成本地在EMS诱变群体中鉴定出发生在特定基因上的点突变。在其基础上发展出的EcoTILLING技术则可发现种质资源中的SNP位点及小插入或缺失多态性位点。水稻是非常重要的粮食作物, 也是已经完成了全基因组序列测定，有丰富的生物信息学资源可以利用的基因组研究模式植物。水稻的分子标记辅助育种将在育种中扮演越来越重要的角色。在这样的背景下，本文从基于特定基因的种质资源鉴定、EMS诱变育种、及水稻功能标记开发等方面论述了其在水稻育种中的应用前景。

TILLING IN RICE BREEDING IN FUTURE

Targeting Induced Local Lesions IN Genomes (TILLING) is a reverse genetics method for functional genomics research.It is possible to screen for point mutations in the populations of EMS mutagenesis with high-throughput and low-cost. EcoTILLING is a method based on TILLING ,was developed for detecting multiple types of polymorphisms in germplasm collections,such as single nucleotide polymorphism,small deletion and insertion etc.Rice is a very important food crop.It is a model plant for genome research also,there are complete genome sequence and a lot of other bioinformatics resources about it.So the marker-assisted breeding is becomeing more and more important in rice breeding.In this paper ,we will discuss some issues based on TILLING about identify germplasm based on gene sequence,EMS mutagenesis breeding,developing functional marker in rice breeding in future.