释放不同种类赤眼蜂对亚洲玉米螟的防治效果比较

【目的】研究适宜黑龙江省第二积温带应用赤眼蜂Trichogramma sp.防治亚洲玉米螟Ostrinia furnacalis (Guenée)的的蜂种及合理的放蜂时期。【方法】2016-2017年,在齐齐哈尔市玉米田人工释放玉米螟赤眼蜂Trichogramma ostriniae、松毛虫赤眼蜂T.dendrolimi和螟黄赤眼蜂T.chilonis防治亚洲玉米螟。于6月30日第1次释放蜂15 000头/hm~2,8月5日第2次释放蜂30 000头/hm~2。【结果】释放不同蜂种田的亚洲玉米螟卵块被寄生率间差异显著,以释放玉米螟赤眼蜂田的寄生率最高,平均为95.7%,比未放蜂对照田的自然寄生率提高了49.0%。其次为松毛虫赤眼蜂和螟黄赤眼蜂,平均寄生率分别为75.0%和71.0%。两年试验结果表明释放3种不同赤眼蜂的玉米田平均被害株率、百株残存虫量和蛀孔数差异显著,综合防治效果以释放玉米螟赤眼蜂最高,为78.1%,其次是松毛虫赤眼蜂和螟黄赤眼蜂,分别为66.2%和60.2%。【结论】玉米螟赤眼蜂是黑龙江省第二积温带防治玉米螟的适宜释放蜂种,第1次放蜂时间提早到6月30日左右。     

赤眼蜂防治玉米螟的应用研究

本文系1974年在山东省邹县进行赤眼蜂防治玉米螟的研究结果。重点是摸索放蜂时间、次数、数量和蜂菌配合的防治效果,同时还探明了玉米的不同播种方式、长势及玉米螟落卵量的不同与放蜂效果的关系。研究结果表明:利用赤眼蜂防治玉米螟效果明显,卵寄生率一般可达70—90%,防治效果达60—80%。赤眼蜂每次放逢量一般以1万头为宜,寄生率可达80%左右,蜂量再多似无必要。放蜂时间要掌握在卵初期,防治效果可达82.6%,若在卵盛期放蜂,防治效果降低34.8%。放蜂的次数主要根据玉米螟全卵期的长短和赤眼蜂在当时的田间温度下发育一代所需时间而确定,一般放蜂3—4次即可。玉米的长势与赤眼蜂寄生率的关系极为密切,长势好的地块赤眼蜂的寄生率明显高于长势差的地块。以蜂灭卵,以菌扫残(幼虫),蜂菌配合可明显提高防治效果。     

化害为利 以虫治虫

我县广大工农兵和科技工作者,在毛主席关于“备战、备荒、为人民”的伟大战略方针指引下,开展了利用松毛虫卵繁殖赤眼蜂防治稻纵卷叶螟的试验,取得了较好的效果。 据盆栽试验,用松毛虫卵培养的松毛虫赤眼蜂和螟黄赤眼蜂对稻纵卷叶螟卵的寄生率都在80％以上。 田间防治第二代稻纵卷叶螟每亩放蜂2—3万头,经6—7天后检查,卵寄生率比对照区高出37.0—41.2％,15天后卷叶虫密度比对照区下降75.4—100％,卷叶率下降60.4—78.3％。赤眼蜂可在稻田内继续繁殖,寄生下一代稻纵卷叶螟的卵。 利用松毛虫卵繁殖赤眼蜂,出蜂正常,蜂体大而活耀。据室内观察,对稻纵卷叶螟卵的寄生率在90％以上。一粒松毛虫卵平均可出螟黄赤眼蜂17.1头,出松毛虫赤眼蜂13.66头。松毛虫卵冷藏15—36天后,仍保持新鲜,不影响寄生率。     

东北地区四种本地赤眼蜂对草地贪夜蛾寄生能力及适应性研究

草地贪夜蛾Spodoptera frugiperda(Smith)是新入侵我国的重大危险性入侵害虫,赤眼蜂是我国生物防治的优势卵寄生蜂,可用于防治多种农林作物上的鳞翅目害虫。为检测东北地区本地赤眼蜂对草地贪夜蛾卵的生防潜能,筛选防治草地贪夜蛾的有效蜂种,本研究比较研究了松毛虫赤眼蜂、玉米螟赤眼蜂、黏虫赤眼蜂和稻螟赤眼蜂对草地贪夜蛾卵的寄生能力和发育适合度。结果表明,4种赤眼蜂均能寄生草地贪夜蛾卵,并完成发育,但其寄生能力和发育适合度存在显著差异。松毛虫赤眼蜂、玉米螟赤眼蜂和黏虫赤眼蜂对草地贪夜蛾卵块寄生率均高于80%,但仅松毛虫赤眼蜂对草地贪夜蛾卵表现出较高的卵粒寄生量。松毛虫赤眼蜂、玉米螟赤眼蜂和黏虫赤眼蜂子代羽化率和雌性比无显著差异,但松毛虫赤眼蜂比其它3种赤眼蜂的发育历期更短,而稻螟赤眼蜂对草地贪夜蛾卵的寄生能力和适合度均最差。研究结果将为应用本地赤眼蜂生物防治潜在入侵东北地区的草地贪夜蛾提供技术支撑。     

利用广赤眼蜂防治玉米螟

1972年我们在较小的范围内利用赤眼蜂防治玉米螟取得了初步成功的基础上,1973年在批林整风运动推动下,在市、县各级领导支持下,全县19个社镇进行了大面积赤眼蜂防治玉米螟的示范,共用柞蚕茧1,100斤,从5月15日开始育蛾采卵,6月15日以卵繁蜂,到7月下旬共繁蜂两亿多头。于7月16日放蜂防治玉米螟(一年一代),放蜂面积达8,000多亩。据几个公社10个大队的360亩玉米地的调查统计,放蜂后玉米螟卵块寄生率平均为96.1％,卵粒寄生率为87.9％,玉米被害株率减少58.7％,越冬残虫量减少64.1％。取得了明显效果,深受贫下中农欢迎。     

米蛾对三种赤眼蜂的适合性及被寄生后卵内游离氨基酸含量的变化

寄生发生前寄生蜂的寄生行为及寄生发生后寄生蜂的生长发育情况能够反映出寄主对寄生蜂的适合性,而寄生蜂对寄主营养物质的吸收和利用是寄生蜂完成发育的生理基础。为了从寄生蜂利用寄主营养能力的角度探讨寄主对不同种赤眼蜂适合性变化的原因,本文观察了以米蛾Corcyra cephalonica Stainton卵为寄主时拟澳洲赤眼蜂Trichogramma confusumViggiani、松毛虫赤眼蜂T. dendrolimi Matsumura和玉米螟赤眼蜂T. ostriniae Pang et Chen的寄生行为及发育和存活情况,测定了被寄生米蛾卵内游离氨基酸的含量 。结果发现,玉米螟赤眼蜂的产卵时间为84.9 s,显著长于拟澳洲赤眼蜂和松毛虫赤眼蜂的产卵时间。拟澳洲赤眼蜂检测寄主所需时间为30.8 s,显著长于玉米螟赤眼蜂和松毛虫赤眼蜂所需时间,但从每寄主卵中羽化出的拟澳洲赤眼蜂数量显著高于松毛虫赤眼蜂及玉米螟赤眼蜂寄生的结果。3种赤眼蜂卵+幼虫的发育历期间不存在显著差异,但卵成虫的发育历期间存在显著差异。玉米螟赤眼蜂幼虫期和预蛹期的死亡率均显著高于拟澳洲赤眼蜂和松毛虫赤眼蜂相应虫期的死亡率。这些结果表明：米蛾卵对松毛虫赤眼蜂及拟澳洲赤眼蜂的适合性高于对玉米螟赤眼蜂的适合性。未被寄生的米蛾卵内游离氨基酸的总量在24～96 h时间段内从开始的2.194 mg/mL逐渐下降到1.565 mg/mL,而被寄生的米蛾卵内游离氨基酸总量均出现先升高后下降的现象。被松毛虫赤眼蜂和拟澳洲赤眼蜂寄生的米蛾卵内游离氨基酸总量在48 h达到最高值,分别为4.239 mg/mL和3.222 mg/mL,被玉米螟赤眼蜂寄生的米蛾在72 h达到最高值,为4 .323 mg/mL,显示同玉米螟赤眼蜂相比,松毛虫赤眼蜂和拟澳洲赤眼蜂能够更快地分解利用寄主营养。这些结果提示,3种赤眼蜂利用米蛾卵内营养物质能力的不同导致了米蛾卵对3种蜂适合性的不同。     

利用自然界害虫卵复壮繁殖赤眼蜂兼治棉田害虫

1975年我们利用赤眼蜂防治棉田三代玉米螟兼治四代棉铃虫获得了良好效果,1976年全大队放蜂面积扩大为1,160亩,其中防治棉田三代玉米螟兼治四代棉铃虫600亩,1977年又增达1,416亩,防治棉田三代玉米螟兼治四代棉铃虫为757亩,先后放蜂三次,每次每亩放蜂一万头左右。在三代玉米螟发生期放蜂于玉米诱集带上,第二、三次放蜂于棉花上,每隔三天放蜂一次。经调查夏玉米诱集带上玉米螟的卵寄生率达100％,棉花上玉米螟的卵寄生率为91％。1976年     

赤眼蜂寄主偏爱可塑性研究

三种赤眼蜂寄主选择性和寄生能力的实验结果表明,松毛虫赤眼蜂对亚洲玉米螟卵的寄主选择性和寄生能力最弱,其次为拟澳洲赤眼蜂,玉米螟赤眼蜂最强.寄主偏爱可塑性实验结果表明,对玉米螟卵选择性和寄生能力较弱的拟澳洲赤眼蜂,其寄主的低偏爱性是可塑的;而对玉米螟卵选择性和寄生能力较强的玉米螟赤眼蜂,在亚洲玉米螟卵繁殖几代后,其寄生能力变化不大.     

利用赤眼蜂防治玉米螟的放蜂新方法

为了进一步提高赤眼蜂放蜂技术和治螟效果,我们于1975年在海林县新合公社斗银大队,设计研究了“重叠放蜂法”、“田间放繁并举放蜂法”,与普通三次放蜂方法的治螟效果比较试验,效果均很理想。这两种新放蜂方法,有三大优点:(1)使赤眼蜂本身适应田间环境,提高生活力;(2)提高螟卵的被寄生率和防治效果;(3)节省劳力,降低成本费用。 一、放蜂方法及蜂卡 加温和放蜂适期 (一)蜂卡加温和放蜂适期指标 在我省东部玉米螟发生一代区,蜂卡加温指标是按照6月底7月初灯下开始见到玉米螟成虫之日起,以及玉米秸堆垛内羽化率达到5％左右时,即开始将     

关于玉米螟卵赤眼蜂繁殖及应用的几个问题

通过几年来的实践,我们对玉米螟卵赤眼蜂的繁殖和应用,初步明确了以下几个问题。 一、蜂种问题 本地蜂种有适应性强、生活力旺盛、寄生率高等优点,因此采集本地玉米螟卵的赤眼蜂作蜂种比较好。一般在8月下旬晚玉米收获前15-20天采集3—4次,将卵块周围叶片剪去,装入大指形管,待羽化后作蜂种。 开始接种繁殖时寄生率往往很低,一般不寄生柞蚕卵;冷藏二、三个月的蓖麻蚕卵,寄生率仅有5％左右,新鲜卵可达20％左右;柳天蛾新鲜卵寄生率可高达40％左右。需连续繁殖二、三代后寄生率才能明显提高,如蓖麻蚕卵为61.7—85.9％,柳天蛾卵为80.6—91.0％。因此,用田间赤眼蜂作蜂种和选择适宜寄主卵很重要。     

Factors influencing parasitism of Trichogramma dendrolimi on eggs of the Asian corn borer, Ostrinia furnacalis

Laboratory studies were made to determine the capacity of Trichogramma dendrolimi to parasitize eggs of Ostrinia furnacalis, as affected by the rearing host species, substrate of host eggs, host age, original locality of host populations, and cold storage of host eggs. Wasps reared from eggs of Antheraea pernyi showed parasitic capacity on eggs of O. furnacalis on average twice as high as that of the wasps reared from eggs of Corcyra cephalonica. When the age of O. furnacalis eggs at 26 °C increased from 0–6 h to 18–24 h, the proportion of wasps that successfully parasitized host eggs, the number of host eggs parasitized, and the rate of parasitization all decreased by >50%. The number of O. furnacalis eggs parasitized per female T. dendrolimi increased with the number of host eggs available, and reached 22.9 in a 24 h period. However, the parasitic capacity of female T. dendrolimi on eggs of O. furnacalis laid on plant leaves was similar to that of O. furnacalis eggs laid on wax paper. Levels of parasitism of O. furnacalis eggs from two widely separated localities, i.e. Changchun (43.50° N, 125.20° E) and Hangzhou (30.18° N, 120.07° E), were similar. Cold storage of O. furnacalis eggs at 4 °C for 5 days did not affect parasitization. Results obtained in this study indicate that although O. furnacalis is a less preferred and less suitable host than many other hosts, such as Dendrolimus punctatus, Actias selene ningpoane, Philosamia cynthia, A. pernyi, C. cephalonica, within the host-species range of T. dendrolimi, the parasitoid has the potential to achieve 50–60% or even higher rates of parasitization of O. furnacalis eggs in corn fields under suitable conditions, and could be used in the biological control of the pest.     

繁育寄主对三种赤眼蜂个体大小及抱卵量的影响

【目的】繁育寄主及成蜂日龄均会影响赤眼蜂体内的抱卵量,为了解繁育寄主及育出赤眼蜂个体大小与赤眼蜂抱卵量的关系,我们在室内研究了米蛾 Corcyra cephalonica（Stainton）卵和亚洲玉米螟 Ostrinia furnacalis （Güenée）卵作为繁育寄主对3种赤眼蜂雌蜂大小、抱卵量的影响。【方法】在光周期14L:10D、温度25±1℃的室内条件下,以米蛾卵、亚洲玉米螟卵作为繁育寄主,解剖不同日龄（羽化后12-72 h）松毛虫赤眼蜂 Trichogramma dendrolimi Matsumura、玉米螟赤眼蜂Trichogramma ostriniae Pang et Chen以及稻螟赤眼蜂Trichogramma japonicum Ashmead,观察雌蜂抱卵量,测量成蜂的大小,分析雌蜂抱卵量与其日龄、个体大小以及繁育寄主的关系【结果】在羽化后48 h以内,3种蜂的抱卵量随雌蜂日龄的增长而增加。羽化后72 h,除米蛾卵繁育的玉米螟赤眼蜂及玉米螟卵繁育的松毛虫赤眼蜂的抱卵量比羽化后48 h时略高外,其他组合均出现下降。以亚洲玉米螟卵为繁育寄主时的玉米螟赤眼蜂的抱卵量显著高于对应日龄的以米蛾卵为繁育寄主时的抱卵量,而松毛虫赤眼蜂（除羽化后12和24 h外）和稻螟赤眼蜂则相反;以米蛾卵为繁育寄主时,相同日龄的赤眼蜂中,松毛虫赤眼蜂抱卵量最高,稻螟赤眼蜂的抱卵量最低;以亚洲玉米螟卵为繁育寄主时,玉米螟赤眼蜂抱卵量最高,稻螟赤眼蜂抱卵量最低。雌蜂抱卵量与雌蜂个体大小呈线性正相关,相关程度随雌蜂日龄增加而更加明显。【结论】后足胫节长度可以作为评价松毛虫赤眼蜂、玉米螟赤眼蜂和稻螟赤眼蜂质量的指标。赤眼蜂抱卵量受繁育寄主、赤眼蜂蜂种及雌蜂日龄的显著影响,寄主的适合性也会影响育出赤眼蜂的大小,筛选合适的繁育寄主有助于提高赤眼蜂的质量。     

Large-scale releases of Trichogramma ostriniae to suppress Ostrinia nubilalis in commercially grown processing and fresh market sweet corn

Abstract:  We evaluated the egg parasitoid Trichogramma ostriniae (Pang et Chen) (Hym.: Trichogrammatidae) for control of European corn borer [Lep.:Crambidae: Ostrinia nubilalis (Hübner)] in fields of commercial processing sweet corn in New York in 2002 and 2003. We made inoculative releases of 75 000 T. ostriniae /ha when corn was at mid-whorl and regional O. nubilalis activity had begun. Each release field was paired with a similar non-release control. The incidence of egg mass parasitism, number of stalk tunnels, incidence of ear damage and effect on insecticide spray decisions were evaluated. Parasitism of cumulative number of O. nubilalis egg masses was 51.3 ± 7.2% and 43.5 ± 5.7% (mean ± SEM) in release fields in 2002 and 2003 respectively. The incidence of ear damage by O. nubilalis was not affected by T. ostriniae in either year of the study. There was no difference in stalk damage between release fields and control fields in either 2002 or 2003. However, individual comparisons between paired release and control fields showed reduced stalk tunnelling in 10 of 19 fields, but ear damage was reduced in only two of 19 fields. Neither the number of actual insecticide sprays nor the number of decisions to spray based on sequential sampling were affected significantly by releasing T. ostriniae . In a separate but related study, where releases were conducted in commercial fresh market sweet corn and used higher rates of release, T. ostriniae had no effect on the number of insecticidal sprays. In an additional experiment using small plots of sweet corn, ear damage was similar whether plots were treated with T. Ostriniae or with insecticide. Additional research should focus on improving the timing, frequency and rate of releases.     

甜玉米地亚洲玉米螟为害的生态控制系统研究

利用自然种群生命表方法 ,研究了甜玉米品种抗虫性差异 ,斯氏线虫、玉米螟赤眼蜂对亚洲玉米螟种群系统的生态控制 ,根据不同卵量水平初步组建了第 5代亚洲玉米螟生态控制系统。结果得出 :亚洲玉米螟为害的生态控制系统由人工生态控制系统和自然控制系统两大部分组成 ,主要通过调控人工生态控制系统中的生态控制措施来达到生态控制目标。当甜玉米品种为穗甜 2号 ,卵密度为 18(块 / 10 0株 )时 ,不采取人为生态控制措施即可控制亚洲玉米螟的为害 ,即在人工生态控制系统内 ,利用穗甜 2号对亚洲玉米螟的抗性即可达到生态控制目标 ;当甜玉米品种为粤甜 1号 ,卵密度为 18或 35 (块 / 10 0株 )时 ,在人工生态控制系统内释放玉米螟赤眼蜂或撒施线虫颗粒剂均可达到生态控制目标 ;当甜玉米品种为粤甜 1号 ,卵密度为 6 2 (块 / 10 0株 )时 ,则要在人工生态控制系统内同时释放玉米螟赤眼蜂和撒施线虫颗粒剂 ,才能达到生态控制目标。通过生态控制系统的建立 ,为甜玉米的无公害化生产提供了一条切实可行的害虫控制途径     

Effectiveness of Trichogramma ostriniae and T. nubilale for controlling the European corn borer Ostrinia nubilalis in sweet corn

Seven field releases of Trichogramma ostriniae and T. nubilale (Hymenoptera: Trichogrammatidae) were made separately and in combination in a sweet corn field to compare the level of parasitism in sentinel eggs of the European corn borer, Ostrinia nubilalis (Lepidoptera: Pyralidae). The results indicate that the levels of egg parasitism among different release dates differed mainly because of changes in weather and plant architecture within the season. The level of egg parasitism by releasing T. ostriniae alone was found to be 15% higher than that by releasing T. nubilale alone, and 20% higher than by releasing the combination of the two species. Further analyses using the logistic regression model for independent and correlated data indicated T. ostriniae to be more efficient at discovering host egg masses and to have higher levels of egg parasitism than T. nubilale. Mutual interference between T. ostriniae and T. nubilale was the main factor for the lower level of egg parasitism when T. ostriniae and T. nubilale were released together. The results suggest that T. ostriniae is the better candidate for augmentative releases for control of the European corn borer, and the two species should not be released into a corn field at the same time.     

Effect of Inoculative Releases of Trichogramma ostriniae on Populations of Ostrinia nubilalis and Damage to Sweet Corn and Field Corn

European corn borer (Ostrinia nubilalis) is one of the most injurious pests of sweet and field corn in the United States. We report here on controlled experiments in which an egg parasitoid (Trichogramma ostriniae) was released inoculatively (75,000 females ha⁻¹) early in the growing season (when corn plants were at the early to mid-whorl stage) to test its efficacy as a biological control agent of O. nubilalis. Releases were made in fields of sweet corn and field corn. Numbers of eggs laid in experimental plots, larval tunnels, and larvae and proportion of damaged ears were determined. Mass of ears was determined for field corn plots. In sweet corn, despite greater oviposition by O. nubilalis in T. ostriniae release plots, the number of borer larvae, stalk tunnels, and damaged ears was reduced by ∼50% compared with those in nonrelease plots. This reduction in damage was consistent for early and late-planted sweet corn. In the field corn plots, larger numbers of O. nubilalis eggs were again laid in some release plots than in control plots. However, O. nubilalis damage appeared to be suppressed in T. ostriniae release plots, although no significant differences were found in most. These results were promising, but further work is required in field corn. The results for sweet corn demonstrated that inoculative releases of T. ostriniae provide suppression of O. nubilalis populations adequate to reduce damage significantly.     

Potential of Trichogramma ostriniae (Hymenoptera: Trichogrammatidae) for biological control of European corn borer (Lepidoptera: Crambidae) in solanaceous crops

We assessed the ability of Trichogramma ostriniae (Peng & Chen) to locate and parasitize Ostrinia nubilalis (Hübner) eggs in crops other than corn, and we evaluated the efficacy of inundative releases of the parasitoid in two solanaceous crops, pepper and potato. Despite a greater plant surface area to search, parasitism of O. nubilalis eggs was consistently higher in sweet corn than dicotyledonous crops such as pepper, snap bean, broccoli, potato, and melon, in choice and no-choice experiments. Nonetheless, in 2002 and 2003, we made four to five separate inundative releases of approximately 30,000-50,000 T. ostriniae per 0.02 ha in nine pepper fields in Virginia, Pennsylvania, and Massachusetts and compared O. nubilalis egg parasitization and fruit damage in those plots with spatially isolated nonrelease plots. Egg parasitization averaged 48.7% in T. ostriniae release plots, which was significantly higher than in nonrelease plots (1.9%). Also, cumulative pepper fruit damage averaged 8.7% in release plots, which was significantly less than in nonrelease plots (27.3%). In potatoes in 2002 and 2003, we made two releases of approximately 75,000 T. ostriniae per 0.2 ha in nine fields in Maine and Virginia and compared O. nubilalis damage in those plots with that in nonrelease plots. T. ostriniae releases significantly reduced the number of tunnel holes and number of O. nubilalis larvae in potato stems. We conclude that this parasitoid has great potential as a biocontrol agent for O. nubilalis in solanaceous crops.     

江苏沿海地区棉田玉米螟综合控制新技术的研究与应用效果

棉田玉米螟综合控制技术研究的新进展包括：改变春玉米的栽培方式降低了棉田玉米螟的虫量基数、开发和筛选新药剂确保了农药品种的更新换代、改进施药方法提高了防治效果、转Bt基因抗虫杂交棉对玉米螟表现很强的抗性功能、棉花生育状态模型和DPC在棉虫综防中的应用丰富了玉米螟综防技术、玉米螟寄生性天敌的调查有助于加强天敌的保护利用,并能提高玉米螟测报的准确性。与常规棉田比较,转Bt基因抗虫杂交棉的推广种植,有效抑制了棉田玉米螟,其残虫量和被害率下降了80％以上;棉田捕食性天教总量增加了25．63％—47．50％;有利于与多种作物间套作;省工节本可增加1476．93元．hm^2,增幅达30％左右、同套复种还可以增加产值15％以上。     

螟长距茧蜂、玉米螟与春玉米间相互关系的初步研究

研究表明,螟长距茧蜂发生高峰期比玉米螟成虫高峰期大约推迟6-9天,与春玉米抽雄期相一致。玉米抽雄开花时绝大部分玉米螟幼虫处在易被螟长距茧蜂寄生的Ⅲ、Ⅳ龄。玉米开花后雄穗的形态结构和营养成分发生改变,致使幼虫下垂转移寻找更适宜的食物。螟长距茧蜂被取食玉米雄穗的玉米螟幼虫所吸引。其寄生与玉米螟幼虫的发育和玉米生育期密切相关。     

Dispersal of Trichogramma ostriniae in field corn

Trichogramma ostriniae has shown success as a biological control agent for European corn borer (Ostrinia nubilalis) in sweet corn and the species offers potential for suppression of lepidopteran pests of field corn. Field corn is typically planted at higher densities, is taller, and has greater leaf area than sweet corn, presenting a possible restriction on T. ostriniae dispersal and efficacy. Therefore, parasitoid dispersal in field corn from the centre of a 6.25 ha square grid was determined using sticky cards to capture adult T. ostriniae and sentinel eggs of O. nubilalis to monitor parasitism after releases of ~1 million of T. ostriniae each into four fields of corn. Dispersal was rapid and extensive, achieving distances of ~175 m within 4–7 days after release. The pattern of movement fit well with a diffusion model of dispersal, with the greatest level of dispersal occurring from 7 to 10 days post-release. Parasitism of O. nubilalis sentinel egg masses declined linearly with distance from the release foci, and was also greatest 7–10 days post-release. However, measurement of association showed no significant differences between the spatial distributions of sticky trap captures and percentage parasitism of O. nubilalis egg masses. The distances from the release point that encompassed 98% of re-captured T. ostriniae increased over time and were estimated to range from a low of 100 m at 4 days post-release to 365 m at 14 days post-release. The results of this research suggest that T. ostriniae relies initially on random movement to locate host patches, and that a single release locus per hectare would be sufficient in field corn.