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1.
Stanislaw Grzesiak Morio Iijima Yasuhiro Kono Akira Yamauchi 《Acta Physiologiae Plantarum》1997,19(3):349-357
Relatively little research has been conducted to determine different responses to drought among cultivars of the legume species.
The objective of this study was to identify differences in seedlings growth, water relations and leaf conductances resulting
from drought imposed on two field bean and two field pea cultivars that had been observed to differ in their drought tolerances,
and special emphasis was placed on the root system development.
Distinct differences between resistant and sensitive cultivars of field bean and field pea became evident in measurements
of the characteristics of the lateral root. The drought treatment induced statistically significant decrease in the number
of the developed laterals, their total length and dry matter. In the drought resistant cultivars (field bean Gobo and field
pea Solara) this reduction was smaller in comparison with sensitive ones (field bean Victor and field pea Bareness). The effect
of drought on growth of tap root in the drought resistant and drought sensitive cultivars was smaller and statistically not
significant. The results showed that drought resistant cultivars when compared with drought sensitive one would demonstrate
less abundance in the above-ground part and greater dimensions of the root system.
The measurements of leaf water potential and stomata diffusive resistance measurements indicate that the physiological reasons
for the different reactions to drought between the resistant and the sensitive field bean and field pea cultivars may be due
to a more effective protection of the level of tissue hydration and due to increase stomata diffusive resistance in the resistant
cultivars. During recovery period it has been also demonstrated that in the drought resistant cultivars a tendency exists
for a more complete return to the level of the control plants. 相似文献
2.
Seasonal leaf water relations characteristics were studied in fully irrigated spring barley (Hordeum distichum L. cv. Gunnar) fertilized at low (50 kg K ha−1) or high (200 kg K ha−1) levels of potassium applied as KCl. The investigation was undertaken from about 14 days before anthesis until the milk ripe
stage in leaves of different position and age. Additionally, the effects of severe water stress on leaf water relations were
studied in the middle of the grain filling period in spring barley (cv. Alis). The leaf water relations characteristics were
determined by the pressure volume (PV) technique.
Water relations of fully irrigated plants were compared in leaf No 7 with the water relations of slowly droughted plants (cv.
Alis). Leaf osmotic potential at full turgor (ψ
π
100
) decreased 0.1 to 0.3 MPa in droughted leaves indicating a limited osmotic adjustment due to solute accumulation. The leaf
osmotic potential at zero turgor (ψ
π
0
) was about −2.2 MPa in fully irrigated plants and −2.6 MPa in droughted plants. The relative water content at zero turgor
(R0) decreased 0.1 unit in severely droughted leaves. The ratio of turgid leaf weight to dry weight (TW/DW) tended to be increased
by drought. The tissue modulus of elasticity (ε) decreased in droughted plants and together with osmotic adjustment mediated
turgor maintenance during drought. A similar response to drought was found in low and high K plants except that the R0 and ε values tended to be higher in the high K plants. Conclusively, during drought limited osmotic adjustment and increase
in elasticity of the leaf tissue mediated turgor maintenance. These effects were only slightly modified by high potassium
application.
The seasonal analysis in fully irrigated plants (cv. Gunnar) showed that within about 14 days from leaf emergence ψ
π
100
decreased from about −0.9 to −1.6 MPa in leaf No 7 (counting the first leaf to emerge as number one) and from about −1.1
to −1.9 MPa in leaf No 8 (the flag leaf) due to solute accumulation. A similar decrease took place in ψ
π
0
except that the level of ψ
π
0
was displaced to a lower level of about 0.2 to 0.3 MPa. Both ψ
π
100
and ψ
π
0
tended to be 0.05 to 0.10 MPa lower in high K than in low K plants. R0 was about 0.8 to 0.9 and was independent of leaf position and age, but tended to be highest in high K plants. The TW/DW ratio
decreased from about 5.5 in leaf No 6 to 4.5 in leaf No 7 and 3.8 in leaf No 8. The TW/DW ratio was 4 to 10% higher in high
K than in low K plants indicating larger leaf cell size in the former. The apoplastic water content (Va) at full turgor constituted about 15% in leaf No 7. ε was maximum at full turgor and varied from about 11 to 34 MPa. ε tended
to be higher in high K plants. Conclusively, in fully watered plants an ontogenetically determined accumulation of solutes
(probably organic as discussed) occurred in the leaves independent of K application. The main effect of high K application
on water relations was an increase in leaf water content and a slight decrease in leaf ψπ.
The effect of K status on growth and drought resistance is discussed. 相似文献
3.
E. S. Jensen 《Plant and Soil》1986,92(1):3-13
Summary The total amount of nitrogen derived from symbiotic nitrogen fixation in two pea and one field bean cultivar, supplied with
50 kg N ha−1 at sowing (‘starter’-N), was estimated to 165, 136, and 186 kg N ha−1, respectively (three-year means). However, estimates varied considerably between the three years. At the full bloom/flat
pod growth stage from 30 to 59 per cent of total N2 fixation had taken place. The proportion of total N derived from N2 fixation at maturity was higher in seeds than in vegetative plant parts and amounted to 59.5, 51.3 and 66.3 per cent of total
above-ground plant N in the two pea cultivars and field bean, respectively (three-year means). The recovery of fertilizer
N was 62.2, 70.2, 52.1, and 69.5 per cent in the two pea cultivars, field bean and barley, respectively. Growth analysis indicated
that barley did not meet the claims for an ideal reference crop in the15N fertilizer dilution technique for estimating N2 fixation in pea and field bean. ‘Starter’-N neither increased the seed yield nor the N content of the grain legumes. 相似文献
4.
Dehydration-induced alterations in growth and osmotic potential of callus from six tepary bean lines varying in drought resistance 总被引:1,自引:0,他引:1
Growth and osmotic potential of calli induced from leaf- and root-derived tissues of six tepary bean lines (Phaseolus acutifolius) varying in drought resistance were assessed in vitro after polyethylene glycol-induced (10%, PEG-10,000) dehydration. Calli of resistant teparies were characterized by low initial osmotic potential (ψs) and relative growth rate (RGR) on medium lacking PEG (−0.30 MPa). However, calli of both resistant and sensitive lines were similar in dry matter percent (DM). Presence of PEG in the medium (−0.58 MPa) elevated DM in all teparies except one resistant line. Both leaf- and root-derived calli of sensitive teparies exhibited osmotic adjustment (OA) but reduced RGR that remained after rehydration in one line. We concluded that preexisting force of low cellular ψs rather than induced OA plays an important role in buffering adverse effects of dehydration and conditioning drought resistance of tepary beans. This information may aid Phaseolus breeders in screening for drought resistance among large number of accessions. 相似文献
5.
U. Celikkol Akcay O. Ercan M. Kavas L. Yildiz C. Yilmaz H. A. Oktem M. Yucel 《Plant Growth Regulation》2010,61(1):21-28
Two cultivars of peanut (Arachis
hypogaea L.) which were designated as resistant (Florispan) and sensitive (Gazipasa) according to their growth retardation under drought
stress conditions were compared for their oxidative damage and antioxidant responses. Sixteen days-old peanut seedlings were
subjected to PEG-6000 solutions of two different osmotic potentials; −0.4 and −0.8 MPa, and various growth parameters, photosystem
II activity, changes in malondialdehyde (MDA), hydrogen peroxide (H2O2) and proline levels, activities of ascorbate peroxidase (APX), catalase (CAT), peroxidase (POX) and gluthatione reductase
(GR) enzymes were determined. Both cultivars exhibited water deficit at −0.8 MPa osmotic potential of PEG-6000 and H2O2 levels significantly increased during exposure to −0.4 MPa osmotic potential. However, H2O2 levels were under control in both cultivars at exposure to −0.8 MPa osmotic potential. Significant proline accumulation was
observed in the tissues of cv. Florispan at −0.8 MPa osmotic potential, whereas proline accumulation did not appear to be
an essential part of the protection mechanism against drought in cv. Gazipasa. No significant variation in chlorophyll fluorescence
values were detected in neither of the cultivars. Enzyme activity measurements revealed that Gazipasa copes well with lesser
magnitudes of drought stress by increasing the activity of mainly APX, and during harsh stress conditions, only APX maintains
its activity in the tissues. In cultivar Florispan, GR activity appears to take role in lesser magnitudes of drought stress,
whereas CAT and APX activities appear to be very crucial antioxidative defenses during intense stress conditions. The results
indicate that, the level of proline and activities of the enzymes CAT and APX are important mechanisms for the maintenance
of drought tolerance in peanut plants. 相似文献
6.
Stanisław Grzesiak Maciej T. Grzesiak Władysław Filek Joanna Stabryła 《Acta Physiologiae Plantarum》2003,25(1):29-37
Effects of soil drought on crop yield of 4 strains and 7 cultivars of spring triticale was investigated under field condition.
The Drought Susceptibility Index (DSI) was evaluated in a two year experiment by the determination of grain loss in conditions
of two soil moisture levels (drought-D and irrigated-IR). In the experiment response to drought was evaluated by different
screening tests (leaf gaseous exchange, leaf water potential, chlorophyll content and fluorescence, leaf injury by drought
and by simulated drought and heat temperature and water loss by excited leaf. The DSI values and the results of screening
tests showed the genetic variation in the degree of drought tolerance. The values of DSI enabled the ranking of the tested
triticale genotypes with respect to their drought tolerance and allow to divide them into three groups of drought susceptibility.
Large differences among studied forms were observed also in changes of leaf water potential, fluorescence and leaf injury.
For plants in vegetative stage of growth the tested breeding forms were easily separated into groups of different drought
tolerance. Changes of ψ, Fv/Fm and LI as a screening tests were the most suitable techniques for estimation of degree of drought
tolerance for triticale. Laboratory screening tests (leaf injury by simulated drought (LIDS) and high temperature (LIHT) and water loss (WL) of excited leaf conducted for nonstressed plants in most cases were significantly correlated with DSI.
The statistically significant correlation between leaf water potential (ψ) was observed only with leaf fluorescence (Fv/Fm).
Changes of Fv/Fm were significantly correlated with ψ, LI and LIHT for 50 °C. Index of leaf injury (LI) by soil drought were significantly correlated with Fv/Fm, LIDS (−1.0, −1.5 MPa), LIHT (45 and 50°C) and water loss (WL). The correlation coefficient between the tests LIDS and LIHT were most of the considered cases statistically significant which indicate that the mechanism of membranes injury resulted
from simulated drought or high temperature were similar in triticale. Water loss (WL) of excited leaves was the most suitable
test for screening drought tolerance in triticale population. Changes of gaseous exchange parameters were not useful as screening
test in this research. 相似文献
7.
Screening for drought tolerance in Sorghum using cell culture 总被引:4,自引:0,他引:4
R. H. Smith S. Bhaskaran F. R. Miller 《In vitro cellular & developmental biology. Plant》1985,21(10):541-545
Summary Callus growth from 10 cultivars ofSorghum bicolor (L.) Moench was measured with increasing levels of polyethylene glycol (PEG) as an osmoticum in the medium to determine whether
differences among these cultivars at the cellular level in response to osmotic stress existed. These cellular ratings were
compared to field ratings from the 10 tolerant-to-susceptible cultivars when grown under drought conditions to determine whether
cellular ratings corresponded to differences in drought tolerance at the plant level. Callus cultures were grown on Murashige
and Skoog inorganic salt formulation plus vitamins, 2,4-dichlorophenoxyacetic acid (2,4-D), kinetin and sucrose, supplemented
with 0 to 25% (wt/vol) PEG corresponding to −0.2 to −1.62 MPa osmotic potential. Results suggest that PEG-induced osmotic
stress on callus cultures can be used to screen sorghum cultivars for potential early field (preflowering) drought tolerance.
This implies that at least a component of the early field drought tolerance in sorghum may have a cellular basis.
This study was supported by U.S. Agency for International Development Grant AID/DSAN/XII/G-0149, and USDA Competitive Grants
Program. 相似文献
8.
Photosynthetic parameters and leaf water potential of five common bean genotypes under mild water deficit 总被引:2,自引:0,他引:2
The leaf water potential, gas exchange and chlorophyll fluorescence were evaluated in five common bean (Phaseolus vulgaris) genotypes A222, A320, BAT477, Carioca and Ouro Negro subjected to moderate water deficit. At the maximum water deficit (10
d of water withholding), the leaf water potential of genotypes A320 and A222 was higher (−0.35 and −0.50 MPa) when compared
to the other genotypes (−0.67 to −0.77 MPa). The stomatal conductance and net photosynthetic rate were significantly reduced
in all genotypes due to the water deficit. The greater reduction in stomatal conductance of A320 under drought resulted in
high intrinsic water use efficiency. Mild water deficit affected the photochemical apparatus in bean genotypes probably by
down-regulation since plants did not show photoinhibition. The photochemical apparatus of A222 and A320 genotypes was more
sensitive to drought stress, showing reduced apparent electron transport even after the recovery of plant water status. On
the other hand, even after 10 d of water withholding, the maximum efficiency of photosystem 2 was not affected, what suggest
efficiency of the photoprotection mechanisms. 相似文献
9.
Ambient temperature and water availability regulate seasonal timing of germination. In fire-prone landscapes, the role of
fire-related cues in affecting the range of temperatures and water potentials (ψs) across which germination can occur is poorly
known, especially in non-Mediterranean landscapes. We examined interactive effects of temperature (15 or 25°C), ψ (0 to −0.9 MPa),
and fire-related cues (heat and smoke) on germination for seeds of three shrub species from fire-prone southeastern Australia.
Incubation temperature affected germination of untreated seeds of Kunzea
ambigua and Kunzea capitata (Myrtaceae) (reduction at 25°C), but germination was uniformly low in Epacris obtusifolia (Ericaceae). Decreasing ψ reduced germination across both incubation temperatures. Fire cues increased germination at both
incubation temperatures and across ψs, although in Kunzea the increase was smaller and occurred over a narrower range of ψs at 25°C. Hydrotime analysis suggested that fire cues reduced
the amount of water necessary for germination of Kunzea seeds. Post-fire germination of the three study species may occur during the warm season, although it is reduced and confined
to wet periods for the two Kunzea species. Warm season germination of the study species is consistent with a trade-off between the increased risk of failure
of a cohort of seedlings, and benefits of early establishment of a cohort that may survive in an environment with aseasonal
rainfall. 相似文献
10.
Germination and seedling growth of pea (Pisum sativum L. cv. Rachna) was studied in salts and PEG-6000 solution having osmotic potentials −0.1, −0.2, −0.3, −0.4 and −0.5 MPa.
At equivalent level of stress, NaCl proved more harmful to germination, seedling growth, vigour index, as well as initial
mobilizing efficiency of food material from seed to the growing seedling, while PEG-6000 was more harmful to imbibition rate
and mobilization efficiency in further days. 相似文献
11.
Poor crop stand is a common problem in saline areas. Germination and seedling emergence may be depressed as a result of impeded
aeration, saline or dry conditions. In this study, we examined the effects of salinity and moisture stress and their interactions
on seed germination and seedling growth of carrots. Variable soil matric and osmotic potentials were either obtained by equilibrating
soil salinized to different degrees on a 0.5 MPa ceramic plate soil moisture extractor or by adding different amounts of salt
solutions to the same mass of air-dried soil, based on a previously determined soil moisture release curve, and allowing to
equilibrate for 1 week.
Germination decreased significantly in the investigated silty soil (Aquic Ustifluvent) at soil moisture potentials higher
than −0.01 MPa, whereas osmotic potentials as low as −0.5 MPa did not influence germination. Matric potentials of −0.3 and
−0.4 MPa, respectively, resulted in a strong decrease (35–95%) of germination and delayed germination by 2 to 5 days in the
silty soil to which different amounts (18 and 36%, respectively) and sizes (0.8–1.2 mm and 1.5–2.2 mm, respectively) of sand
particles had been added. No effect of sand and grain diameter was detected. Germination was not affected by comparable osmotic
potentials.
Seedling growth showed a much higher sensitivity than germination to decreasing matric potentials, but was not affected by
osmotic potentials ranging from −0.05 to −0.5 MPa. Optimum shoot growth occurred at matric potentials between −0.025 and −0.1
MPa. Shoot and root growth decreased markedly at matric potentials higher than −0.01 MPa. Fresh weight of shoots decreased
gradually at matric potentials lower than −0.2 MPa. Root growth was significantly increased at matric potentials of −0.1 to
−0.3 MPa, whereas comparable osmotic potentials did not have equivalent effects.
It is concluded that germination and seedling growth are differently affected by comparable matric and osmotic stresses and
that water stress exerts a more negative effect than salt stress. 相似文献
12.
To assess the potential of short-term screenings for drought resistance at the seedling stage to detect ecotypic variation
and predict field performance, we studied the responses to water deficit of seedlings of Pinus canariensis from five geographic origins under controlled conditions and compared these responses with the performance of provenances
in a multi-site field trial. Leaf water potential, the osmotic component, leaf chlorophyll fluorescence and growth and biomass
partitioning were measured as seedlings were subjected for 11 days to two levels of osmotic potential generated by polyethylene
glycol (PEG 6000), −1 MPa (slowly imposed water deficit; S) and −1.5 MPa (fast imposed water deficit; F), and a control treatment
(no PEG added to the nutrient solution; C). Leaf water potential declined to final mean values of −1.2, −2.7 and −4.7 MPa
in the C, S and F treatments, respectively. The ratio of variable to maximum chlorophyll fluorescence declined to final mean
values of 0.77, 0.66 and 0.40 in the C, S and F treatments, respectively, with no differences amongst provenances. All provenances
showed an active osmotic adjustment (OA) in response to water deficit which varied depending on the drying rate. A slow imposition
of water deficit favoured solute accumulation. Pooling all treatments, the index of OA ranged from 0.28 to 0.40, but rose
considerably when only C and S treatments were considered (0.56 to 0.70). There was a positive and significant correlation
between the overall index of OA (all treatments pooled) and the drought period in the site of origin, suggesting ecotypic
variation in OA as a result of drought duration. Seedlings allocated more dry matter to roots than shoots when subjected to
moderate and slowly imposed water deficit; only one provenance showed no increase in the root to shoot ratio at the end of
the treatment period compared with control seedlings. Responses to controlled water deficits were only qualitatively related
to performance (survival and growth) of provenances in several field sites, indicating the involvement of complex mechanisms
to cope with drought under natural conditions. However, the provenance with the highest overall index of OA outgrew and outsurvived
the other provenances in the most arid site, and the only provenance not modifying the root to shoot ratio in response to
water deficit survived the least in all field sites. Acclimation of root to shoot ratio and net solute accumulation to water
deficit could hence favour drought-tolerance beyond the seedling stage and be used as preliminary predictors of field performance. 相似文献
13.
M. V. B. Figueiredo H. A. Burity C. R. Martínez C. P. Chanway 《World journal of microbiology & biotechnology》2007,23(2):187-193
A greenhouse experiment was carried out aiming to evaluate the response to drought stress of cowpea nodule enzymatic activities
during different plant developmental stages leading to biological N2 fixation. Stress was applied by controlling soil’s water-potential through a porous cup. Cowpea plants cv IPA 205 were grown
in pots with yellow latosol soil under three different matric potential (ψm) treatments. Even with high evaporative demand and limited soil water availability, cowpea could not induce an extremely
low leaf water potential (ψw). Sap ureides concentration in cowpea declined during the drought stress period. There was a decline in enzyme activity in
the metabolic pathways concerned with N2 fixation: NADH-dependent glutamate synthase (EC 1.4.1.14), glutamine synthetase (EC 6.3.1.2) and phosphoenolpyruvate carboxylase
(EC 4.1.1.31). In contrast, an increase in glutamate dehydrogenase (EC 1.4.1.4) was observed as the ψm declined. Metabolism associated with N2 assimilation was impaired every time that the ψw was reduced below −0.73 MPa as had happened in the stressed treatments. The stress applied by the porous cup was gradual
and the plant recovered its turgor, avoiding permanent deleterious alterations in the cellular metabolism, even from a limited
cowpea-growth ψm. 相似文献
14.
普通菜豆种质资源芽期抗旱性鉴定 总被引:9,自引:2,他引:7
李龙 《植物遗传资源学报》2013,14(4):600-605
摘要:干旱是影响我国普通菜豆生产的主要因素之一,筛选芽期抗旱性种质资源,培育抗旱品种,有利于提高普通菜豆品种的出苗率和幼苗长势,对发展我国普通菜豆生产具有重要意义。本研究首先以4份普通菜豆种质为材料,检测了不同渗透势PEG6000溶液模拟旱胁迫下的发芽率和发芽势,确定了PEG6000溶液的最适渗透势为-0.7MPa(浓度为19.6%);以-0.7MPa的PEG6000溶液对121份普通菜豆种质进行芽期模拟旱胁迫,测定发芽率、发芽势、下胚轴长、胚根长、干重和鲜重等10项指标;通过主成分分析筛选出相对发芽率、相对发芽势、相对鲜重、相对干重、相对胚根长,相对总芽长,相对胚根/下胚轴指数、相对发芽指数、相对活力指数等9项指标可以有效评价普通菜豆的芽期抗旱性;利用隶属函数分析法对121份种质的芽期抗旱性进行综合评价,筛选出跃进豆(F0000156)、白扁豆(F0000613)等芽期抗旱性种质,为普通菜豆抗旱生理与机制研究、抗旱育种奠定了基础。 相似文献
15.
Sorghum (Sorghum bicolor L. Moench) plants were grown in solution culture and stressed at three rates of decreasing leaf water potential (−0.123,
−0.068 and −0.029 MPa day−1) achieved by the incremental addition of an osmoticum, polyethylene glycol (PEG) 6000 to the solutions. Plants were also
grown in soil and given different amounts of water which resulted in rates of decreasing leaf water potentials of −0.130 and
−0.073 MPa day−1.
The rate of stress and the culture system influenced the accumulation of solutes in the cell, but not cell volume. A rapid
stress (−0.123 and −0.130 MPa day−1) to approximately −1.6 MPa leaf water potential resulted in 0.75 and 0.16 MPa of osmotic adjustment in the PEG and soil culture
respectively. At moderate stress (−0.068 and −0.073 MPa day−1) respective values were 1.68 and 0.58 MPa. There were some visual symptoms in the solution grown plants characteristic of
uptake of high molecular weight PEG. However the relative growth rates of these plants were equal to or greater than those
of the soil grown plants.
In view of the differences in plant water status of soil and PEG solution cultured plants it was concluded that the use of
the latter system would not be entirely suitable for some studies of drought resistance in sorghum, as related to crop performance
in the field. 相似文献
16.
Isa A. M. Yunusa Sue E. Thomson Keith P. Pollock Lu Youwei Donald J. Mead 《Plant and Soil》2005,275(1-2):195-206
In order to understand how radiata pines respond to declining supply of soil-water in agroforestry systems, we monitored water
potential in xylem (ψ
x
), osmotic potential (ψ) and relative water content (q) for fascicles at pre-dawn and at mid-day for 3-year-old trees that were raised from either seedlings (Seedling) or from
tissue culture (TC3 and TC4), and grown either alone (Control) or over lucerne (Medicago sativa) pasture (Lucerne). Water relations at dawn were mostly similar for all the pines, except late in the season when ψ was lower,
bulk turgor pressure (P), deduced as the difference between ψ
x
and ψ, was higher, for TC3 than for the other two pines. At mid-day, Seedling often had higher ψ
x
and ψ, but because of its poor osmotic adjustment (OA) had lower P, than either TC3 or TC4. The cell walls were more elastic in Seedling with modulus of elasticity (e) of 6.5 MPa compared with 8.1 MPa for both TC3 and TC4, while loss of turgor was estimated to occur at ψ
x
of −1.45 MPa for Seedling, −1.38 MPa for TC3 and −1.35 MPa for TC4. All trees irrespective of their origin had higher ψ
x
, P, CO2 assimilation (A), and stomatal conductance (g
s
), but lower ψ, in Control than in Lucerne in which the soil profile was consistently drier. The trends in ψ
x
, ψ, q and A did not reflect the known differences in dry weight of trees, P was in the order TC3 > TC4 > Seedling, consistent with previously reported tree weights. Both TC3 and TC4 had higher P, due to their larger OA, than Seedling, although the latter had higher A. Thus ψ
x
and A that are routinely measured may not always adequately explain differences in growth amongst pines; it is advisable that ψ
be determined to allow deductions of P be made when using water relations to analyse plant growth. 相似文献
17.
Stanisław Grzesiak 《Acta Physiologiae Plantarum》2001,23(4):443-456
Effects of soil drought on growth and productivity of 16 single cross maize hybrids were investigated under field and greenhouse
experiments. The Drought Susceptibility Index (DSI) was evaluated in a three year field experiment by the determination of
grain loss in conditions of two soil moisture levels (drought and irrigated) and in a pot experiment by the effects of periodical
soil drought on seedling dry matter. In the greenhouse experiment response to drought in maize genotypes was also evaluated
by root to shoot dry mater ratio, transpiration productivity index, indexes of kernel germination and index of leaf injury
by drought and heat temperature. The obtained values of DSI enabled the ranking of the tested genotypes with respect to their
drought tolerance. The values of DSI obtained in the field experiment allow to divide the examined genotypes into three, and
in the greenhouse experiment into two groups of drought susceptibility. The correlation coefficients between the DSI of maize
hybrids in the field and the greenhouse experiments was high and statistically significant, being equal to 0.876. The ranking
of hybrids drought tolerance, identified on the basis of field experiments was generally in agreement with the ranking established
on the basis of the greenhouse experiment. In the greenhouse experiment statistically significant coefficients of correlation
with DSI values in hybrids were obtained for the ratio of dry matter of overground parts to dry matter of roots, both for
control and drought treatments, whereas in the estimation of the transpiration productivity coefficient and total dry matter
the correlation coefficients were not statistically significant. In this study several laboratory tests were carried out for
the drought tolerance of plants (kernel germination, leaf injury) on 4 drought resistant and 4 drought sensitive maize hybrids.
Statistically significant correlation coefficients between DSI and the examined parameter of grain germination and leaf injury
were obtained for the determination of promptness index (PI), seedling survival index (SS) and leaf injuries indexes (IDS,
ITS) as a result of exposure to 14 days of soil drought, osmotic drought −0.9 MPa and exposure to high temperature 45 ° or
50 °C. The results of laboratory tests show that in maize the genetic variation in the degree of drought tolerance is better
manifested under severe conditions of water deficit in the soil. 相似文献
18.
The Development of Desiccation-tolerance and Maximum Seed Quality During Seed Maturation in Six Grain Legumes 总被引:3,自引:0,他引:3
Physiological maturity, i.e. the time when seedsreach their maximum dry weight during development, occurredwhen maturation drying on the parent plant in the field hadreduced seed moisture content to approximately 60 per cent infaba bean (Vicia faba L.), lentil (Lens culinaris Medic.), chickpea(Cicer arietinum L.), white lupin (Lupinus albus L.), soya bean(Glycine max [L.] Merr.) and pea (Pisum sativum L.) The onsetof desiccation-tolerance, i.e. the ability of seeds to germinatefollowing harvest and rapid artificial drying, coincided withphysiological maturity, except in pea where it occurred a littleearlier at about 70 per cent moisture content. Maximum seedquality as determined by maximum viability, minimum seedlingabnormalities and maximum seedling size occurred in pea, chickpeaand lupin when seeds were harvested for rapid drying at physiologicalmaturity; but for maximum seed quality in the other speciesmaturation drying had to proceed further - to about 45 per centmoisture content in soya bean and to about 30 per cent moisturecontent in lentil and faba bean seed crops. Much of this variationamongst the six species, however, was due to differences inthe variation in maturity within each seed crop. Results forindividual pods showed that peak maturity, i.e. maximum seedquality following harvest and rapid artificial drying, was achievedin all six species once maturation drying had reduced the moisturecontent of the seeds to 4550 per cent. In pea, faba beanand soya bean there was a substantial decline in viability andan increase in seedling abnormalities when harvest was delayedbeyond the optimal moisture content for harvest. 相似文献
19.
The responses of leaf water parameters to drought were examined using three sunflower (Helianthus annuus L.) genotypes. Osmotic potential at full water saturation (π100), apoplastic water fraction (AWF) and bulk elastic modulus (BEM) were determined by pressure-volume curve analysis on well
watered or on water-stressed plants (−1.0 MPa Ψ1 < −1.5 MPa) previously drought-pretreated or not. The drought-pretreated plants were subjected to a 7-day drought period
(predawn leaf water potential reached −0.9 MPa) followed by 8 days of rewatering. In well watered plants, all genotypes in
response to drought acclimation displayed a significantly decreased π100 associated with a decrease in the leaf water potential at the turgor-loss point (decrease in Ψtlp was between 0.15 and 0.21 MPa, depending on the genotype). In two genotypes, drought acclimation affected the partitioning
of water between the apoplastic and symplastic fractions without any effect on the total amount of water in the leaves. As
a third genotype displayed no modification of AWF and BEM after drought acclimation, the decreased π100 was only due to the net accumulation of solutes and was consistent with the adjustment of the photochemical efficiency observed
previously in this genotype in response to drought acclimation. In water-stressed plants, the osmotic adjustment (OA) can
increase further beyond that observed in response to the drought pretreatment. However, the maintenance of photosynthetic
rate and stomatal conductance at low leaf water potentials not only depends on the extent of osmotic adjustment, but also
on the interaction between OA and AWF or BEM. Adaptative responses of leaf water parameters to drought are thus quite contrasted
in sunflower genotypes.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
20.
Several QTLs involved in osmotic-adjustment trait variation in barley (Hordeum vulgare L.) 总被引:4,自引:0,他引:4
B. Teulat D. This M. Khairallah C. Borries C. Ragot P. Sourdille P. Leroy P. Monneveux A. Charrier 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,96(5):688-698
Osmotic adjustment (OA) was previously demonstrated to be an important adaptive mechanism of drought tolerance in cereals.
In order to determine which genomic regions are involved in OA variation, 187 barley (Hordeum vulgare L.) recombinant inbred lines (RILs) derived from a cross between Tadmor (drought tolerant) and Er/Apm (susceptible) were
studied in a growth chamber for their OA capacity (through correlated traits and by calculation), at an early growth stage
and under two water treatments (soil moisture of 14% and 100% of field capacity). The continuous distribution of the traits
and their broad-sense line heritabilities, ranging from 0.04 to 0.44, indicated that OA and related traits should have a polygenic
nature. A subset of 167 RILs were also genotyped using 78 RFLP, 32 RAPD and three morphological markers and a linkage map
was constructed. Despite strong environmental effects acting on the traits, interval mapping and single-marker ANOVA allowed
the detection of three QTLs for relative water content (RWC), four QTLs for osmotic potential (ψπ), two QTLs of osmotic potential at full turgor (ψπ100) and one QTL for osmotic adjustment at a soil moisture of 14% field capacity. For the irrigated treatment, only two QTLs
were detected: one for RWC and one for ψπ100. Two chromosomal regions were involved in several OA-related trait variations and could be considered as regions controlling
OA; these were present on chromosome 1 (7H) and chromosome 6 (6H), whereas other regions were specific for one trait. No major
QTL was found. However, the genomic region involved in OA-related traits on chromosome 1 (7H) in barley seemed to be conserved
for OA variation among cereals. Epistatic effects, with or without additive effects, acted on the traits.
Received: 15 July 1997 / Accepted: 29 October 1997 相似文献