Endogenous opiates and behavior: 2014

This paper is the thirty-seventh consecutive installment of the annual review of research concerning the endogenous opioid system. It summarizes papers published during 2014 that studied the behavioral effects of molecular, pharmacological and genetic manipulation of opioid peptides, opioid receptors, opioid agonists and opioid antagonists. The particular topics that continue to be covered include the molecular-biochemical effects and neurochemical localization studies of endogenous opioids and their receptors related to behavior (endogenous opioids and receptors), and the roles of these opioid peptides and receptors in pain and analgesia (pain and analgesia); stress and social status (human studies); tolerance and dependence (opioid mediation of other analgesic responses); learning and memory (stress and social status); eating and drinking (stress-induced analgesia); alcohol and drugs of abuse (emotional responses in opioid-mediated behaviors); sexual activity and hormones, pregnancy, development and endocrinology (opioid involvement in stress response regulation); mental illness and mood (tolerance and dependence); seizures and neurologic disorders (learning and memory); electrical-related activity and neurophysiology (opiates and conditioned place preferences (CPP)); general activity and locomotion (eating and drinking); gastrointestinal, renal and hepatic functions (alcohol and drugs of abuse); cardiovascular responses (opiates and ethanol); respiration and thermoregulation (opiates and THC); and immunological responses (opiates and stimulants).This paper is the thirty-seventh consecutive installment of the annual review of research concerning the endogenous opioid system. It summarizes papers published during 2014 that studied the behavioral effects of molecular, pharmacological and genetic manipulation of opioid peptides, opioid receptors, opioid agonists and opioid antagonists. The particular topics that continue to be covered include the molecular-biochemical effects and neurochemical localization studies of endogenous opioids and their receptors related to behavior (endogenous opioids and receptors), and the roles of these opioid peptides and receptors in pain and analgesia (pain and analgesia); stress and social status (human studies); tolerance and dependence (opioid mediation of other analgesic responses); learning and memory (stress and social status); eating and drinking (stress-induced analgesia); alcohol and drugs of abuse (emotional responses in opioid-mediated behaviors); sexual activity and hormones, pregnancy, development and endocrinology (opioid involvement in stress response regulation); mental illness and mood (tolerance and dependence); seizures and neurologic disorders (learning and memory); electrical-related activity and neurophysiology (opiates and conditioned place preferences (CPP)); general activity and locomotion (eating and drinking); gastrointestinal, renal and hepatic functions (alcohol and drugs of abuse); cardiovascular responses (opiates and ethanol); respiration and thermoregulation (opiates and THC); and immunological responses (opiates and stimulants).     

Endogenous opiates and behavior: 2007

This paper is the thirtieth consecutive installment of the annual review of research concerning the endogenous opioid system. It summarizes papers published during 2007 that studied the behavioral effects of molecular, pharmacological and genetic manipulation of opioid peptides, opioid receptors, opioid agonists and opioid antagonists. The particular topics that continue to be covered include the molecular-biochemical effects and neurochemical localization studies of endogenous opioids and their receptors related to behavior, and the roles of these opioid peptides and receptors in pain and analgesia; stress and social status; tolerance and dependence; learning and memory; eating and drinking; alcohol and drugs of abuse; sexual activity and hormones, pregnancy, development and endocrinology; mental illness and mood; seizures and neurologic disorders; electrical-related activity and neurophysiology; general activity and locomotion; gastrointestinal, renal and hepatic functions; cardiovascular responses; respiration and thermoregulation; and immunological responses.     

夏枯草药材和种植土壤中农药及重金属残留分析

采用气相色谱及ICP-AES法测定了安徽庐江和江苏洪泽2个种植基地的土壤和夏枯草(Prunella vulgaris L.)果穗及全草中有机氯农药及重金属含量,并根据污染指数和相关标准对土壤及药材的安全性进行了评价.测定结果表明:来源于2个基地的土壤及药材中有机氯农药及重金属含量有明显差异.庐江产果穗和全草中Pb、Cd、Cu、Cr、As及BHC含量分别为3.361和3.953、0.172和0.190、8.258和7.722、3.423和2.658、0.284和0.355、0.003和0.004 mg·kg-1,Hg和DDT未检出;洪泽产果穗和全草中Pb、Cd、Cu、Cr、Hg及BHC含量分别为2.399和1.558、0.155和0.111、7.682和6.756、4.259和3.801、0.077和0.102、0.003和0.006 mg·kg-1,As未检出,果穗中也未检出DDT.庐江基地土壤中Cd、Cu、Cr、As、Hg、BHC和DDT含量分别为0.001、12.943、47.417、1.008、0.003、0.003和0.002 mg·kg-1,Pb未检出;洪泽基地土壤中Pb、Cd、Cu、Cr、As、Hg和BHC含量分别为3.443、0.002、18.655、63.385、3.701、0.141和0.004 mg·kg-1,DDT未检出.比较结果表明:夏枯草果穗中重金属残留量均高于全草,但均低于国家限量标准;土壤中有机氯农药及重金属单项污染指数均小于1,且庐江和洪泽基地土壤的综合污染指数分别为0.286和0.399,因此,土壤污染等级属安全级且污染水平为清洁级.     

Using yeast two-hybrid system to detect interactions of ATP synthase subunits from Spinacia oleracea

Subunit interactions among the chloroplast ATP synthase subunits were studied using the yeast two-hybrid system. Various pairwise combinations of genes encoding a, p, y, 8 and e subunits of Spinach ATP synthase fused to the binding domain or activation domain of GAL4 DNA were introduced into yeast and then expression of a reporter gene encoding p-galactosidase was detected. Of all the combinations, that of y and e subunit genes showed the highest level of reporter gene expression, while those of a and p, a and e, p and e and p and 8 induced stable and significant reporter gene expression. The combination of 8 and e as well as that of 8 and y induced weak and unstable reporter gene expression. However, combinations of a and y, p and y and a and 8 did not induce reporter gene expression. These results suggested that specific and strong interactions between y and e, a and p, a and e, p and e and p and 8 subunits, and weak and transient interactions between 8 and e and 8 and y subunits occurred in the yeast     

广西岑溪县七坪林区土壤微生物生态分布及生化活性的研究

广西气候温和,雨量充沛,亚热带和热带森林资源十分丰富。为了合理利用和开发广西梧州、玉林等地区南亚热带的土地资源和充分发挥该地区森林资源的经济效益,1987年我们     

NOK在骨肉瘤细胞系MG63 EMT过程中的作用

目的:通过对比和检测人正常成骨细胞系hFOB1.19以及骨肉瘤细胞系MG63中NOK以及EMT标志性分子E-cadherin、Vimitin的mRNA和蛋白表达量,并观察NOK对骨肉瘤细胞系MG63中EMT标志性分子E-cadherin及Vimitin的mRNA和蛋白表达量的影响,探讨NOK在骨肉瘤细胞系MG63 EMT过程中的作用。方法:qRT-PCR、Western blot法检测人正常成骨细胞系hFOB1.19以及骨肉瘤细胞系MG63中NOK、E-cadherin、Vimitin的mRNA和蛋白表达量;构建慢病毒并干扰骨肉瘤细胞系MG63中NOK表达,qRT-PCR、Western blot法检测干扰NOK前后EMT标志性分子E-cadherin及Vimitin的mRNA和蛋白表达。结果:相比于人正常成骨细胞系,NOK、Vimitin的mRNA和蛋白在骨肉瘤细胞系MG63中高表达,E-cadherin的mRNA和蛋白在骨肉瘤细胞系MG63中低表达。慢病毒干扰骨肉瘤细胞中NOK表达后,E-cadherin的mRNA和蛋白表达升高,Vimitin的mRNA和蛋白表达降低。结论:NOK具有促进骨肉瘤细胞系MG63发生EMT过程。     

SHAPING THE IMMIGRATION DEBATE: CONTENDING CIVIL SOCIETIES ON THE US–MEXICO BORDER

This article explores the cultural politics of memory and the reconfiguration of commemorative landscapes as one of the principal arenas within which the interiority and exteriority of place and identity are being re-narrated, contested, and re-enacted by actors and institutions representing a wide range of scalar stances. We treat place and identity as mutually constituted, dynamically interactive discursive and practical categories of becoming, and identify borderlands as multiscalar sites of imminence, where the interiority and exteriority of place and identity are re-narrated and re-enacted. Using a multiscalar network approach, we focus on the actors engaged in the cultural politics of memory in the southern Russian-Estonian borderlands. We conclude that borderlands are central multiscalar nodes where power, place and identity intersect, where the interior and exterior not only of Setomaa and Seto-ness, but also of Estonia and Estonian-ness, Russia and Russian-ness, and Europe and European-ness are reconfigured.     

铜、镉胁迫下施硫肥和有机肥对冬小麦碳氮运转的影响

采用盆栽试验,研究了铜、镉胁迫条件下施硫和有机肥对冬小麦碳氮运转的影响。结果表明,与各自对照相比,铜、镉胁迫下低施硫和有机肥的处理增加了小麦叶片、茎鞘、颖壳穗轴等营养器官花前贮藏物质、氮素的再运转量和运转率以及营养器官花前贮藏物质、氮素的总再运转量和总运转率,高施硫和有机肥的铜、镉处理则规律性不明显。在铜、镉胁迫条件下,施用硫肥和有机肥处理增加了小麦成熟期籽粒重和花后光合同化物输入籽粒量以及籽粒氮素含量和花后氮素积累量。与各自对照相比,铜胁迫下施硫和有机肥的处理与镉胁迫下低施硫和有机肥的处理增加了成熟期小麦的穗数、穗粒数和千粒重,提高了籽粒产量,其中以T\-5处理增产幅度最大;镉胁迫下高施硫和有机肥的处理则变化不大。铜、镉胁迫下低施硫和有机肥的处理均增加了籽粒淀粉含量,而高施硫和有机肥的铜、镉处理则未表现出此规律。此外,铜、镉胁迫下施硫和有机肥的各处理增加了籽粒蛋白质的含量。     

Comparative Evaluation of Trace Metal Distribution and Correlation in Human Malignant and Benign Breast Tissues

Selected trace metals were analyzed in human malignant and nonmalignant (benign) breast tissue samples by the flame atomic absorption spectrophotometric method. In malignant tissues, dominant mean concentrations were revealed by Na, K, Ca, Mg, Fe, Zn, and Al at 927, 552, 231, 61.7, 36.5, 18.3, and 8.94 microg/g, respectively, while the mean metal levels in benign tissues were 903, 435, 183, 63.3, 24.7, 14.5, and 10.1 microg/g, respectively. Average concentrations of Cd, Co, Cr, Cu, Fe, Mn, K, Ca, and Zn were noted to be significantly higher in the malignant tissues compared with the benign tissues. Significantly strong correlations (r > 0.50) in malignant tissues were observed between Mn and Co, Mn and Cd, Cd and Cr, Fe and Mn, Cd and Co, Fe and Co, Mg and Pb, Cd and Fe, Mg and Ni, Pb and Ni, Ni and Sr, and Fe and Pb, whereas, Cd and Co, Cd and Mn, Co and Mg, Co and Mn, Cu and Mn, Co and Ni, Mg and Ni, Cd and Cu, Cd and Ni, Ca and Mg, Mn and Pb, Cu and Ni, Fe and Ni, Cd and Mg, Co and Cu, Cr and Na, and Cd and Cr revealed strong and significant relationships in benign tissues at p < 0.001. Principal component analysis of the metals data yielded six principal components for malignant tissues and five principal components for benign tissues, with considerably different loadings, duly supported by cluster analysis. The study revealed a considerably different pattern of distribution and mutual correlations of trace metals in the breast tissues of benign and cancerous patients.     

桃果实不同发育时期细胞内糖酸分布对甜酸风味的影响

为了解桃果实发育过程中细胞内糖酸的分布、变化规律对果实甜酸风味的影响,采用区室分析方法研究了‘白凤’桃(Prunus persica‘Hakuho’)果实不同发育时期细胞内糖酸组分、含量及其分布对甜酸风味的影响。结果表明,成熟果实中(花后100 d)可溶性糖(蔗糖、葡萄糖、果糖和山梨醇)在液泡、细胞质和细胞间隙中的含量分别为27.3、11.6、9.0 mg/g,有机酸(苹果酸、柠檬酸、奎宁酸和莽草酸)含量为2.09、0.94、0.35 mg/g;未成熟果实中(花后60 d)可溶性糖在液泡、细胞质和细胞间隙中的含量分别为0.97、2.2、2.3 mg/g,有机酸含量为0.25、0.44、0.82 mg/g。‘白凤’桃果肉细胞内不同的糖酸分布对成熟果实的甜酸风味具有显著影响,而对未成熟果实影响较小。成熟果实中糖酸在液泡、细胞质和细胞间隙三者之间的分布差异可能是导致果实甜度变化的主要原因。     

Gene-activation mechanisms in the regression of atherosclerosis, elimination of diabetes type 2, and prevention of dementia

Atherosclerotic vascular disease, diabetes mellitus (DM) and dementia are major global health problems. Both endogenous and exogenous factors activate genes functioning in biological processes. This review article focuses on gene-activation mechanisms that regress atherosclerosis, eliminate DM type 2 (DM2), and prevent cognitive decline and dementia. Gene-activating compounds upregulating functions of liver endoplasmic reticulum (ER) and affecting lipid and protein metabolism, increase ER size through membrane synthesis, and produce an antiatherogenic plasma lipoprotein profile. Numerous gene-activators regress atherosclerosis and reduce the occurrence of atherosclerotic disease. The gene-activators increase glucose disposal rate and insulin sensitivity and, by restoring normal glucose and insulin levels, remove metabolic syndrome and DM2. Patients with DM2 show an improvement of plasma lipoprotein profile and glucose tolerance together with increase in liver phospholipid (PL) and cytochrome (CYP) P450. The gene-activating compounds induce hepatic protein and PL synthesis, and upregulate enzymes including CYPs and glucokinase, nuclear receptors, apolipoproteins and ABC (ATP-binding cassette) transporters. They induce reparation of ER structures and eliminate consequences of ER stress. Healthy living habits activate mechanisms that maintain high levels of HDL and apolipoprotein AI, promote health, and prevent cognitive decline and dementia. Agonists of liver X receptor (LXR) reduce amyloid in brain plaques and improve cognitive performance in mouse models of Alzheimer's disease. The gene activation increases the capacity to withstand cellular stress and to repair cellular damage and increases life span. Life free of major health problems and in good cognitive health promotes well-being and living a long and active life.     

Total C and N Pools and Fluxes Vary with Time,Soil Temperature,and Moisture Along an Elevation,Precipitation, and Vegetation Gradient in Southern Appalachian Forests

The interactions of terrestrial C pools and fluxes with spatial and temporal variation in climate are not well understood. We conducted this study in the southern Appalachian Mountains where complex topography provides variability in temperature, precipitation, and forest communities. In 1990, we established five large plots across an elevation gradient allowing us to study the regulation of C and N pools and cycling by temperature and water, in reference watersheds in Coweeta Hydrologic Laboratory, a USDA Forest Service Experimental Forest, in western NC, USA. Communities included mixed-oak pine, mixed-oak, cove hardwood, and northern hardwood. We examined 20-year changes in overstory productivity and biomass, leaf litterfall C and N fluxes, and total C and N pools in organic and surface mineral soil horizons, and coarse wood, and relationships with growing season soil temperature and precipitation. Productivity increased over time and with precipitation. Litterfall C and N flux increased over time and with increasing temperature and precipitation, respectively. Organic horizon C and N did not change over time and were not correlated to litterfall inputs. Mineral soil C and N did not change over time, and the negative effect of temperature on soil pools was evident across the gradient. Our data show that increasing temperature and variability in precipitation will result in altered aboveground productivity. Variation in surface soil C and N is related to topographic variation in temperature which is confounded with vegetation community. Data suggest that climatic changes will result in altered aboveground and soil C and N sequestration and fluxes.     

Endogenous opiates and behavior: 2001

This paper is the twenty-fourth installment of the annual review of research concerning the opiate system. It summarizes papers published during 2001 that studied the behavioral effects of the opiate peptides and antagonists. The particular topics covered this year include the molecular-biochemical effects and neurochemical localization studies of endogenous opioids and their receptors (Section 2), and the roles of these opioid peptides and receptors in pain and analgesia (Section 3); stress and social status (Section 4); tolerance and dependence (Section 5); learning and memory (Section 6); eating and drinking (Section 7); alcohol and drugs of abuse (Section 8); sexual activity and hormones, pregnancy, development and endocrinology(Section 9); mental illness and mood (Section 10); seizures and neurologic disorders (Section 11); electrical-related activity and neurophysiology (Section 12); general activity and locomotion (Section 13); gastrointestinal, renal and hepatic functions (Section 14); cardiovascular responses (Section 15); respiration and thermoregulation (Section 16); and immunological responses (Section 17).     

恢复生态学研究的一些基本问题探讨

对恢复生态学的研究概况、基本概念、内涵与研究内容以及生态恢复的目标、原则、程序与技术进行了分析与探讨。指出恢复生态学应加强基础理论研究(包括生态系统的演替理论及干扰条件下生态系统的受损过程与响应机制研究等)和应用技术研究(包括土壤、水体、大气和植被恢复技术、生物多样性保护技术以及生态系统的组装与集成技术等).生态恢复与重建是指根据生态学原理,通过一定的生物、生态以及工程的技术,人为地切断生态系统退化的主导因子和过程,调整和优化系统内部及其与外界的物质、能量和信息的流动过程及其时空秩序,使生态系统的结构、功能和生态学潜力尽快地成功地恢复到原有的乃至更高的水平。     

Water and electrolyte changes in skeletal and cardiac muscles of rats during prolonged hypokinesia

The objective of this study was to show that hypokinesia (diminished movement) could affect differently water and electrolyte content in muscles having minimum differences in their function and morphology. To this end, we studied water and electrolyte content in skeletal and cardiac muscles, fluid excretion, electrolyte absorption, and electrolyte levels in plasma, urine and feces of rats during prolonged hypokinesia (HK). Studies were conducted on one-hundred-twenty-six 13-weeks old male Wister rats during a pre-hypokinetic period and a hypokinesia period. Animals were equally divided into two groups: vivarium control rats (VCR) and hypokinetic rats (HKR). Hypokinetic animals were kept in small individual cages which restricted their movements in all directions without hindering food and water intake. Control rats were housed in individual cages under vivarium control conditions. Sodium (Na+) and potassium (K+) absorption, electrolyte and water content in cardiac muscles (right and left ventricle), thigh extensor (quadriceps femoris muscle) and long muscle of the back (biceps femoris muscle), urine volume, and electrolyte levels in plasma and urine and feces did not change in VCR when compared to their pre-hypokinetic levels. The absorption of Na+ and K+, water and electrolyte content in cardiac and skeletal muscles decreased significantly, while urine volume, plasma electrolyte levels and urine and fecal electrolyte excretion increased significantly in HKR compared with their pre-HK values and with their respective vivarium control (VCR). Water and electrolyte content decreased more significantly in skeletal than in cardiac muscles. Water and electrolyte levels decreased more in the thigh extensor and in the right ventricle than in the long muscle of the back, the left ventricle or the septum. Muscles suffering from higher water and electrolyte loss against the background of lower water and electrolyte content show lower water and electrolyte deposition. Lower electrolyte and water content in skeletal than in cardiac muscle shows that water and electrolyte content decreases more in skeletal than cardiac muscles. Skeletal muscle showed lower water and electrolyte content than cardiac muscle indicating that the risk for decreased muscle water and electrolyte content is inversely related to the muscle function and morphology, i.e., the more weight-bearing supporting function and morphology muscles have, the higher the risk for lower muscle water and electrolyte content. It was concluded that the greater muscle function and morphology, the lower electrolyte and water deposition, the higher water and electrolyte losses, and the lower water and electrolyte content.     

Endogenous opiates and behavior: 2012

This paper is the thirty-fifth consecutive installment of the annual review of research concerning the endogenous opioid system. It summarizes papers published during 2012 that studied the behavioral effects of molecular, pharmacological and genetic manipulation of opioid peptides, opioid receptors, opioid agonists and opioid antagonists. The particular topics that continue to be covered include the molecular-biochemical effects and neurochemical localization studies of endogenous opioids and their receptors related to behavior (Section 2), and the roles of these opioid peptides and receptors in pain and analgesia (Section 3); stress and social status (Section 4); tolerance and dependence (Section 5); learning and memory (Section 6); eating and drinking (Section 7); alcohol and drugs of abuse (Section 8); sexual activity and hormones, pregnancy, development and endocrinology (Section 9); mental illness and mood (Section 10); seizures and neurologic disorders (Section 11); electrical-related activity and neurophysiology (Section 12); general activity and locomotion (Section 13); gastrointestinal, renal and hepatic functions (Section 14); cardiovascular responses (Section 15); respiration and thermoregulation (Section 16); and immunological responses (Section 17).     

中国14个特殊旁系族群的头面部特征比较

头面部特征是人类学各人种进行分类的重要依据,在人类学的研究中被用作亲缘关系的证据。2006-2016年在四川、云南、西藏、贵州、海南、新疆、内蒙古共调查14个族群成人2989人（男性1434人,女性1555人）的16项头面部指标,比较这些族群头面部特征差异。研究结果如下：1）在男性族群中木雅人、尔苏人、临高人、白马人的面宽、头宽均较大。图瓦人、布里亚特人、摩梭人的面宽、头宽、形态面高、鼻高值均较大。僜人、革家人、莽人的面宽、头宽较小,而形态面高、鼻高值较大。空格人的面宽、头宽、形态面高、鼻高值均较小。2）在女性族群中图瓦人、布里亚特人的头宽、面宽、鼻高、耳上头高均较大。革家人、空格人的头宽、面宽、鼻高、耳上头高均较小。木雅人、尔苏人、八甲人、白马人、夏尔巴人的头宽、面宽较大,鼻高、耳上头高较小。僜人、克木人、莽人的头宽、面宽较小,而鼻高、耳上头高较大。3）头宽、容貌耳长跟纬度、年平均气温、年降雨量等环境因子相关性较高。4）通过聚类分析和主成分分析木雅人、尔苏人、白马人与羌族头面部特征比较接近。克木人与佤族头面部特征最为接近。革家人、僜人、莽人头面部特征比较接近。图瓦人、布里亚特人头面部特征与蒙古族比较接近。     

Baboon carboxylesterases 1 and 2: sequences, structures and phylogenetic relationships with human and other primate carboxylesterases

Background  Carboxylesterase (CES) is predominantly responsible for the detoxification of a wide range of drugs and narcotics, and catalyze several reactions in cholesterol and fatty acid metabolism. Studies of the genetic and biochemical properties of primate CES may contribute to an improved understanding of human disease, including atherosclerosis, obesity and drug addiction, for which non-human primates serve as useful animal models.
Methods  We cloned and sequenced baboon CES1 and CES2 and used in vitro and in silico methods to predict protein secondary and tertiary structures, and examined evolutionary relationships for these enzymes with other primate and mouse CES orthologs.
Results and Conclusions  We found that baboon CES1 and CES2 proteins retained extensive similarity with human CES1 and CES2, shared key structural features reported for human CES1, and showed family specific sequences consistent with their multimeric and monomeric subunit structures respectively.     

城市林业概述与发展趋势

随着社会经济的快速发展和城市化进程的加快,城市环境问题日益成为社会各界关注的焦点,城市林业在城市建设与发展中的地位和作用越来越受到重视。从自然属性与社会属性,地域范围与层次的角度介绍了城市林业的基本概念和内涵,并比较了城市林业与传统林业、现代园林在属性、经营管理和功能方面的差别。借助已有的研究数据和成果,阐述了城市林业在城市发展中的作用,提出了现代城市林业面临的机遇和挑战以及未来发展趋势。     

(Co)variance components, genetic parameters and annual trends for calf weights in a Brahman herd kept on floodable savanna

(Co)variance components and genetic parameters were estimated for body weights of an elite Brahman herd under a designed, supervised management and genetic program, including strategic artificial insemination (AI). Restricted maximum likelihood methods were used with a univariate animal model for birth weight (BW) and a bivariate model for weaning weight (205-day weight, 205W) and 18-month weight (548-day weight, 548W). Models included random animal direct and maternal genetic effects, maternal permanent environmental effect (c2), and sex-year-month of birth-age of dam and genetic group (identified and unidentified paternity), as fixed effects. Analysis A1 included all calves and analysis A2 included only those with identified sires. Of the 8,066 calves born, 36% were progeny of AI, 11% from single sire and 53% from multi-sire herds. They were born from 1985 to 1998, from 2559 dams and 146 sires (78 identified). Estimates of direct, maternal and total heritabilities from A1 for BW, 205W and 548W were: 0.23, 0.07 and 0.30; 0.08, 0.14 and 0.16; 0.16, 0.04 and 0.28, respectively. Corresponding estimates of direct maternal genetic correlations were 0.22, 0.07 and 0.86, and c2 estimates were 0.04, 0.14 and 0.04, respectively. Estimates of direct and maternal genetic, and permanent environmental correlations between 205W and 548W were: 0.66, 0.70 and 1.00. Variances and genetic parameters from A1 and A2 were, in general, very similar. Estimates of phenotypic, and direct and maternal genetic trends per year from A1 were: 0.393, 0.004 and 0.003 kg (BW), 3.367, 0.142 and 0.115 kg (205W), 1.813, 0.263 and 0.095 kg (548W). Estimates of direct and maternal genetic trends from A2 were: 0.033 and -0.002 kg (BW); 0.186 and 0.276 kg (205W); 0.471 and 0.136 kg (548W). The modern selection methods that have been used recently should be continued, with emphasis on the improvement of cow efficiency for sustainable beef production on floodable savanna combined with improved pasture.