基于树干液流技术的北京市刺槐冠层吸收臭氧特征研究

全球范围内加速的城市化导致空气质量严重退化。随着北京市建设范围不断扩大和机动汽车数量迅猛增长,空气污染日益严重。浓度不断增加的近地层臭氧作为影响全球气候变化的重要因素和危害人类健康、动植物生长的二次污染物,受到广泛关注。城市树木能够有效地去除大气污染物,进而提高空气质量。目前已有很多研究关于区域尺度上城市树木吸收臭氧,然而,冠层尺度上城市树木吸收臭氧特征少有研究。因此,本文基于树干液流技术,结合天气变化和大气臭氧浓度分析,研究夏秋季节北京市典型绿化树种刺槐（Robinia pseudoacacia）整树冠层吸收臭氧特征及环境影响因素。结果表明,在日尺度上,刺槐吸收臭氧速率变化呈单峰曲线,于下午15:00左右达到峰值;夏季峰值范围较宽,秋季峰值范围较窄;中午前后累积吸收臭氧量增加最明显。在季节尺度上,夏季刺槐吸收臭氧速率高于秋季;夏季累积吸收臭氧量显著增加,秋季略有增加。刺槐吸收臭氧的时间变化规律取决于大气臭氧浓度和冠层对臭氧的导度。臭氧浓度日变化和季节变化明显,导致刺槐吸收臭氧速率时间变化格局与之接近。在一定的臭氧浓度下,刺槐吸收臭氧速率的变化主要由冠层对臭氧的导度调控,进而受水汽压亏缺和总辐射的影响。随着水汽压亏缺降低,刺槐冠层对臭氧的导度明显下降;总辐射大于600 W/m2,冠层对臭氧的导度迅速下降。研究树种刺槐单位冠层投影面积上年吸收臭氧量约为0.16 g/m2,明显低于基于模型得到的结果,表明评估森林受臭氧危害的风险应考虑树种冠层臭氧通量。     

森林生态系统硅素循环研究进展

硅是植物生长发育的有益元素,其在生态系统内的迁移转化是维持生态系统结构与功能的决定性因素之一。近年来,陆地生态系统硅循环特别森林生态系统硅循环在全球生物地球化学循环中的重要性,受到越来越多的关注。该文总结了国内外森林生态系统硅循环研究的成果,在综述了硅在森林生态系统中的存在形态、分布、循环过程的基础上,总结了森林生态系统硅循环的特点、作用及其影响因素,并指出典型森林生态系统类型中硅循环规律的研究、森林生态系统与其它生态系统硅循环的比较研究、森林生态系统硅循环对全球气候变化的影响和响应研究和人类干扰对森林生态系统硅循环的影响的研究将是今后开展森林生态系统硅循环研究的重点。     

森林生态系统细根周转规律及影响因素

根系周转是陆地生态系统碳循环的关键过程, 对研究土壤碳库变化及全球气候变化均具有重要意义。然而由于根系周转率的测量计算方法较多, 不同方法得出的结果差异较大, 且目前对全球区域尺度上森林生态系统根系周转的研究还不够充分, 使得全球森林生态系统根系周转变化规律仍不清楚。该研究通过收集文献数据并统一周转率计算方法, 对全球5种森林类型的细根周转空间格局进行整合, 同时结合土壤理化性质和气候数据, 得出影响森林生态系统细根周转的因子。结果表明, 不同森林类型细根周转率存在显著差异, 且随着纬度的升高逐渐降低; 森林生态系统细根周转率与年平均温度和年平均降水量呈正相关; 森林生态系统细根周转率与土壤有机碳含量呈正相关但与土壤pH值呈负相关。该研究为揭示森林生态系统细根周转规律及机制提供了科学依据。     

森林生态系统细根周转规律及影响因素

根系周转是陆地生态系统碳循环的关键过程,对研究土壤碳库变化及全球气候变化均具有重要意义。然而由于根系周转率的测量计算方法较多,不同方法得出的结果差异较大,且目前对全球区域尺度上森林生态系统根系周转的研究还不够充分,使得全球森林生态系统根系周转变化规律仍不清楚。该研究通过收集文献数据并统一周转率计算方法,对全球5种森林类型的细根周转空间格局进行整合,同时结合土壤理化性质和气候数据,得出影响森林生态系统细根周转的因子。结果表明,不同森林类型细根周转率存在显著差异,且随着纬度的升高逐渐降低;森林生态系统细根周转率与年平均温度和年平均降水量呈正相关;森林生态系统细根周转率与土壤有机碳含量呈正相关但与土壤pH值呈负相关。该研究为揭示森林生态系统细根周转规律及机制提供了科学依据。     

陆地生态系统水分利用效率对气候变化的响应研究进展

气候变化显著影响陆地生态系统生产力以及水分利用格局,而水分利用效率(Water Use Efficiency,WUE)是衡量生态系统碳水耦合关系的重要指标之一。研究陆地生态系统水分利用效率对气候变化的响应,有助于深入理解生态系统的变化规律,模拟和预测生态系统碳水过程的发展状况,从而为应对全球气候变化提供新的依据。为了更好地掌握生态系统水分利用效率研究现状以及其对温度、CO2等关键气候因子的响应情况,本文总结了陆地生态系统水分利用效率对气候变化响应的最新研究进展。首先介绍了相关的定义并归纳了两种不同计算方式的差异和特点;接着重点总结了陆地生态系统水分利用效率对大气温度、CO2、水分、干旱以及太阳辐射等影响因素的响应;最后文章总结了目前3个相关的研究态势,主要包括:(1)长时间序列水分利用效率与气候要素的关系研究;(2)土地利用/覆被变化对水分利用效率的影响及其对气候的反馈研究;(3)多尺度水分利用效率综合研究。本研究可为深入研究生态系统过程对气候变化的响应提供参考。     

森林干扰度评价

干扰普遍存在于森林生态系统,是森林群落演替的驱动力之一。干扰对森林生态系统的影响程度,决定于干扰的频率、强度、空间范围等,过度频繁或不合理的干扰,可能导致森林生态系统的毁灭。森林对干扰的响应表现在多方面,其内在联系十分复杂,因此,很难用森林中某个现象或某几个现象反映森林生态系统对干扰的响应。为了准确、科学地评价现有森林生态系统对干扰的响应程度,本文提出应用森林干扰度（REFD）评价现有森林受干扰的程度。森林干扰度是由于干扰的存在造成森林生态系统结构与功能的改变程度,仅反映干扰后现有森林与目标森林（地带性顶极植被或原有森林等）的距离程度（差距）,不反映干扰的种类、强度、性质等因素;在对森林干扰度内涵进行详细分析的基础上,确定了评价森林干扰度的原则及不同尺度条件下森林干扰度的评价指标。     

气候变化对雌雄异株植物影响的研究进展

雌雄异株植物作为陆地生态系统的重要组成部分,在维持生态系统结构和功能稳定性方面发挥着关键作用。但是,由于雌雄异株植物的不同性别植株在形态特征、生理特征和资源分配等方面均存在明显差异,至今对全球气候变化背景下雌雄植物的性别差异响应机制的认识仍十分有限。本文综述了全球变暖、降水变化和大气CO2浓度升高等主要气候因子变化对雌雄异株植物的影响,探讨了雌雄异株植物对气候变化响应的性别差异,提出了未来雌雄异株植物与气候变化关系研究的重点,以期为揭示陆地生态系统结构和功能稳定性对气候变化的响应机制提供参考依据。     

森林生态系统碳水关系及其影响因子研究进展

森林生态系统的碳水关系是陆地生态系统碳循环和水循环相互耦合的作用过程,对研究森林碳汇、森林生态水文过程和全球变化响应有重要意义.在全球变化背景下,森林生态系统碳水关系已成为生态水文学领域中的一个热点科学问题.本文在总结国际上森林碳汇研究的基础上,概述了森林碳水关系的过程机制,包括森林水分利用效率、不同尺度上的碳水关系、尺度推绎和碳水关系的模拟研究方面的进展;总结了影响森林碳水关系的因子和研究进展,包括水分条件、CO₂浓度升高、增温、氮沉降、臭氧浓度变化、辐射因子和海拔梯度因子对森林碳水关系的影响;最后对已有研究存在的问题进行了初步分析,并对未来研究内容和方向进行了展望.     

用于测定陆地生态系统与大气间CO2交换通量的多通道全自动通量箱系统

陆地生态系统与大气间CO2交换是全球碳循环的最重要组成部分,科学地测定其CO2交换通量一直是陆地生态系统碳循环研究的核心工作之一。提高观测的效率和减少观测对自然的干扰,是科学精确地估算区域和全球尺度上的陆地生态系统与大气间CO2交换量的关键。在参考国内外已有的陆地生态系统与大气间CO2交换通量箱式法观测技术的基础上,发展了一套多通道全自动通量箱系统用来连续观测陆地生态系统或土壤与大气间的CO2交换通量。在黄土高原中国科学院长武农业生态试验站的麦田和苹果园中进行了系统测试,结果表明,该系统不但能够实现自动、连续、多点观测,而且对自然环境的影响比较小,在田间的实验观测中,该系统运行稳定,能够比较客观地得到陆地生态系统与大气间的CO2交换通量。     

近30年中国陆地生态系统NDVI时空变化特征

气候变化已明显影响到陆地植被的活动,但在不同生态系统间存在差异,研究不同陆地生态系统归一化植被指数(NDVI)的时空变化特征,不仅可揭示各生态系统植被活动对气候变化的响应规律,而且可为我国不同生态区制定应对气候变化的策略和生态文明建设提供科学依据。基于1982—2012年GIMMS NDVI3g和中国陆地生态系统类型数据,利用一元线性回归、集合经验模态分解和相关分析等方法,研究了近30年中国各陆地生态系统NDVI的时空变化特征,分析了其与气候事件的关系。结果表明,近30年中国植被活动显著上升,年平均归一化植被指数(ANDVI)的上升幅度为0.0029/10a(P0.05),年最大归一化植被指数(MNDVI)的上升幅度为0.0076/10a(P0.01);植被活动显著增强的区域主要是分布在东部季风区的农田和森林生态系统,显著下降的区域主要是分布于西北的荒漠生态系统和东北的森林生态系统;尽管ANDVI和MNDVI线性趋势的显著性有所差异,但农田、森林、草地和水体与湿地生态系统的NDVI总体呈非稳定的上升趋势,上升过程中伴随着较大波动,荒漠生态系统的NDVI呈下降趋势,植被退化显著;与线性趋势不同,各生态系统植被活动的残差趋势包含"上升—下降"两个阶段,并相继于20世纪90年代到21世纪初发生转折;上述5类生态系统的植被活动存在不同尺度的周期特征,年际周期波动特征(1.9—7.6a)比较显著,而年代际周期(10.7a和22.2a)的显著性相对较差;各生态系统的空间异质性在趋强过程中存在2.1—7.1a的年际周期节律;海洋与大气环流的短周期脉动与各生态系统植被活动的周期性节律有着明显关联,ENSO事件和太阳活动是推动植被活动周期性振荡的重要因素。     

Unambiguous through-bond sugar-to-base correlations for purines in 13C, 15N-labeled nucleic acids: The HsCsNb,HsCs(N)bCb,and HbNbCb experiments

Summary A set of three 3D (¹H, ¹³C, ¹⁵N) triple-resonance correlation experiments has been designed to provide H1-H8 intraresidue sugar-to-base correlations in purines in an unambiguous and efficient manner. Together, the H_sC_sN_b, H_sC_s(N)_bC_b, and H_bN_bC_b experiments correlate the H1 sugar proton to the H8 proton of the attached base by means of the {H1, C1, N9, C8, H8} heteronuclear scalar coupling network. The assignment strategy presented here allows for unambiguous H1-H8 intraresidue correlations, provided that no two purines have both the same H1 and C1 chemical shifts and the same C8 and N9 chemical shifts. These experiments have yielded H1-H8 intraresidue sugar-to-base correlations for all five guanosines in the [¹³C, ¹⁵N] isotopically labeled RNA duplex r(GGCGCUUGCGUC)₂.     

Crystal structure of the ternary FimC-FimF(t)-FimD(N) complex indicates conserved pilus chaperone-subunit complex recognition by the usher FimD

Type 1 pili, anchored to the outer membrane protein FimD, enable uropathogenic Escherichia coli to attach to host cells. During pilus biogenesis, the N-terminal periplasmic domain of FimD (FimD(N)) binds complexes between the chaperone FimC and pilus subunits via its partly disordered N-terminal segment, as recently shown for the FimC-FimH(P)-FimD(N) ternary complex. We report the structure of a new ternary complex (FimC-FimF(t)-FimD(N)) with the subunit FimF(t) instead of FimH(p). FimD(N) recognizes FimC-FimF(t) and FimC-FimH(P) very similarly, predominantly through hydrophobic interactions. The conserved binding mode at a "hot spot" on the chaperone surface could guide the design of pilus assembly inhibitors.     

μ- and μ4-η coordination of A2H2 (A = C, Si, Ge, Sn and Pb) ligands with transition metals

The heavier analogs of C₂H₂ have been studied at the B3LYP level for their μ and μ₄-η² coordination properties with the transition metals. Based on known alkyne compounds, transition metal fragments [W₂(μ-NH)(Cp)₂(Cl)₂] and [Fe₄(CO)₁₂] have been chosen. The SBKJC relativistic effective core potentials and their associated basis sets were used on W, Fe, Sn and Pb, and the 6-31G(d) basis set was used on all other elements. All the complexes of Si₂H₂, Ge₂H₂, Sn₂H₂ and Pb₂H₂ are found to be local minima. The trans-twist nature of the ligand A₂H₂ (A = Si-Pb) is large in μ-coordinated complexes of W, and it is very small in μ₄-η² coordinated complexes of Fe. The electronic structure of these complexes was investigated using fragment molecular orbital method (FMO).     

Introduction: A Close Look at the Vacuolar ATPase

The vacuolar ATPases (V-type ATPases) are a family of ATP-dependent ion pumps and found in two principal locations, in endomembranes and in plasma membranes. This family of ATPases is responsible for acidification of intracellulare compartments and, in certain cases, ion transport across the plasma membrane of eucaryotic cells. V-ATPases are composed of two distinct domains: a catalytic V₁ sector, in which ATP hydrolysis takes place, and the membrane-embedded sector, V₀, which functions in ion conduction. In the past decade impressive progress has been made in elucidating the properties structure, function and moleculare biology. These knowledge sheds light also on the evolution of V-ATPases and their related families of A-(A₁A₀-ATPase) and F-type (F₁F₀-ATPases)ATPases.     

Acetylcholinesterase/Butyrylcholinesterase inhibition activity of some new carbacylamidophosphate deriviatives

Eight newly synthesized carbacylamidophosphates with the general formula RC(O)NHP(O)Cl₂ with R = pCl–C₆H₄ 1a, pBr–C₆H₄ 2a, C₆H₅ 3a, and pMe–C₆H₄ 4a and RC(O)NHP(O)(NC₄H₈O)₂ R = pCl–C₆H₄ 1b, pBr–C₆H₄ 2b, C₆H₅ 3b, pMe–C₆H₄ 4b, were selected to compare the inhibition kinetic parameters, IC₅₀, K_i, k_p and K_D, on human erythrocyte acetylcholinesterase (hAChE) and bovine serum butyrylcholinesterase (BuChE), Also, the in vivo inhibition potency of compound 2a, 2b and 3a, were studied. The data demonstrates that compound 2a and compound 2b are the potent sensitive as AChE and BuChE inhibitors respectively, and the inhibition of hAChE is about 10-fold greater than that of BuChE.     

Earthworm effects on gaseous emissions during vermifiltration of pig fresh slurry

Treatment of liquid manure can result in the production of ammonia, nitrous oxide and methane. Earthworms mix and transform nitrogen and carbon without consuming additional energy. The objective of this paper is to analyse whether earthworms modify the emissions of NH₃, N₂O, CH₄ and CO₂ during vermifiltration of pig slurry.The experiment used mesocosms of around 50 L, made from a vermifilter treating the diluted manure of a swine house. Three levels of slurry were added to the mesocosms, with or without earthworms, during one month, in triplicate. Earthworm abundance and gas emissions were measured three and five times, respectively.There was a decrease in emissions of ammonia and nitrous oxide and a sink of methane in treatments with earthworms. We suggest that earthworm abundance can be used as a bioindicator of low energy input, and low greenhouse gas and ammonia output in systems using fresh slurry with water recycling.     

Antibacterial properties of recombinant human non-pancreatic secretory phospholipase A2

Human non-pancreatic secretory phospholipase A₂ (hnpsPLA₂) is a group IIA phospholipase A₂ which plays an important role in the innate immune response. This enzyme was found to exhibit bactericidal activity toward Gram-positive bacteria, but not Gram-negative ones. Though native hnpsPLA₂ is active over a broad pH range, it is only highly active at alkaline conditions with the optimum activity pH of about 8.5. In order to make it highly active at neutral pH, we have obtained two hnpsPLA₂ mutants, Glu89Lys and Arg100Glu that work better at neutral pH in a previous study. In the present study, we tested the bactericidal effects of the native hnpsPLA₂ and the two mutants. Both native hnpsPLA₂ and the two mutants exhibit bactericidal activity toward Gram-positive bacteria. Furthermore, they can also kill Escherichia coli, a Gram-negative bacterium. The two mutants showed better bactericidal activity for E. coli at neutral pH than the native enzyme, which is consistent with the enzyme activities. As hnpsPLA₂ is highly stable and biocompatible, it may provide a promising therapy for bacteria infection treatment or other bactericidal applications.     

Crystal structure of the archaeal A1Ao ATP synthase subunit B from Methanosarcina mazei Gö1: Implications of nucleotide-binding differences in the major A1Ao subunits A and B

The A1Ao ATP synthase from archaea represents a class of chimeric ATPases/synthases, whose function and general structural design share characteristics both with vacuolar V1Vo ATPases and with F1Fo ATP synthases. The primary sequences of the two large polypeptides A and B, from the catalytic part, are closely related to the eukaryotic V1Vo ATPases. The chimeric nature of the A1Ao ATP synthase from the archaeon Methanosarcina mazei G?1 was investigated in terms of nucleotide interaction. Here, we demonstrate the ability of the overexpressed A and B subunits to bind ADP and ATP by photoaffinity labeling. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry was used to map the peptide of subunit B involved in nucleotide interaction. Nucleotide affinities in both subunits were determined by fluorescence correlation spectroscopy, indicating a weaker binding of nucleotide analogues to subunit B than to A. In addition, the nucleotide-free crystal structure of subunit B is presented at 1.5 A resolution, providing the first view of the so-called non-catalytic subunit of the A1Ao ATP synthase. Superposition of the A-ATP synthase non-catalytic B subunit and the F-ATP synthase non-catalytic alpha subunit provides new insights into the similarities and differences of these nucleotide-binding ATPase subunits in particular, and into nucleotide binding in general. The arrangement of subunit B within the intact A1Ao ATP synthase is presented.     

Assessing the Impact of Advected African Dust on Air Quality and Health in the Eastern United States

Large quantities of African dust are carried into the southeastern United States each summer with concentrations typically in the range of 10 to 100?µg m^?3. Because approximately one-third to one-half of the dust mass is in the size range under 2.5?µm diameter, the advection of African dust has implications for the EPA's newly implemented standard for PM_2.5 particulate matter and for the assessment of human health effects. It will be difficult to assess the impact of African dust events on air quality because they occur during the summer (maximum in July) when photochemical pollution events are frequent and intense in the eastern United States. Indeed, the presence of dust could affect the evolution of photochemical dust events. In order to assess the role of African dust in air quality in the United States, it will be necessary to develop a set of diagnostic indicators; it appears that dust mineralogy and elemental composition might be useful for this purpose. Various satellite products can be used to characterize the spatial coverage of dust events and, when coupled with meteorological tools, to predict impact regions.