中国倍叉(责)属三新种(责翅目:叉(责)科)

记述我国倍叉(责)属Amphinemura 3新种: 尖突倍叉(责)Amphinemura acutata sp. nov.、双突倍叉(责)Amphinemura didyma sp. nov.、单突倍叉 (责)Amphinemura singularis sp. nov..     

中国叉(虫责)属二新种(襀翅目,叉(虫责)科)

记述我国叉(虫责)属Nemoura 2新种:吉林叉(虫责)N.ijlinensis sp.nov.、妙峰山叉(虫责)N.miaofengshanensis sp.nov.,描述了其形态特征并与近似种做了比较.     

贵州钩(虫责)属一新种记述((襀)翅目:(虫责)科)

记述采自贵州宽阔水保护区的钩(虫责)属l新种:巨刺钩(虫责)Kamimuria grandispinata Du ＆Sun,sp.nov.,该新种的阳茎特征与长刺钩虫责K.longispina Wu相似,但长刺钩(虫责)阳茎囊背面近端部有1膜质突起,上面生有粒状微刺,而新种阳茎囊背面近端部是1个近似三角形的刺斑,无突起...     

中国瘤(虫责)属一新种记述(襀翅目:(虫责)科)

记述采自中国陕西佛龙的瘤(虫责)属Tyloperla 1新种:双凹瘤(虫责)Tyloperla bihypodroma Du, sp. nov.,该种与尖突瘤(虫责)Tyloperla attenuata很相近,但前者第8背板近后缘形成1个隆起脊、其中部有1凹陷,后者则在背板中部形成1近圆形的隆起、其上的锥状感觉器比前者多;前者第9背板中央微凹、其前缘骨化明显、极小的锥状感觉器稀少,后者中部有1丛明显的锥状感觉器;前者翅透明,后者翅微烟褐色.新种的模式标本保存在扬州大学昆虫标本馆.     

西藏倍叉属(襀翅目:叉科)三新种(英文)

记述我国西藏倍叉属Amphinemura 3新种 :杨氏倍叉Amphinemurayangisp .nov .(模式产地 :鲁郎 )、李氏倍叉Amphinemuraliisp .nov .(模式产地 :米林 )、西藏倍叉Amphinemuratibetensissp .nov .(模式产地 :波密 )。杨氏倍叉A .yangisp .nov .与a .wittmeriZwick&Sivec近似 ,但肛侧突中叶末端小而缘且有 3— 5个粗刺 ;李氏倍叉A .liisp .nov .与A .lebeziSivec近似 ,但肛侧突内叶不对称 ;西藏倍叉A .tibetensissp .nov .与江苏倍A .kiangsiensis(Wu)近似 ,但内叶短于中叶且末端呈 2瓣状。模式标本存中国农业大学昆虫标本馆。     

中国陕西诺(虫责)种团(襀翅目,卷(虫责)科)一新种记述

记述宁夏卷1新种,即龙潭诺Rhopalopsole longtana sp.nov.,并讨论了其与近似种的区别。模式标本保存在中国农业大学博物馆。龙潭诺,新种Rhopalopsole longtana sp.nov.(图1～8)新种与秦岭诺R.qinlinga Sivec&Harper,2008近似,肛侧突大型,具强骨化的刺突,同属于陕西诺种团。二者的主要区别在于:新种雄虫第9背板后缘中部有1隆起的横脊和三角形刺突,其两侧各有1个向后延伸的叶状突,肛上突端部三角形。而秦岭诺雄虫第9背板无刺突,后缘略平直,肛上突端部圆钝。正模♂,宁夏泾源龙潭,2008-07-05,刘经贤采。词源:新种种名以采集人的姓氏命名。     

中国瘤[虫责]属一新种记述（襀翅目：蟥科）

记述采自中国陕西佛龙的瘤[虫责]属Tyloperla1新种：双凹瘤[虫责]Tyloperla bihypodroma Du,sp．nov．,该种与尖突瘤[虫责]Tyloperla attenuata很相近,但前者第8背板近后缘形成1个隆起脊、其中部有1凹陷,后者则在背板中部形成1近圆形的隆起、其上的锥状感觉器比前者多;前者第9背板中央微凹、其前缘骨化明显、极小的锥状感觉器稀少,后者中部有1丛明显的锥状感觉器;前者翅透明,后者翅微烟褐色。新种的模式标本保存在扬州大学昆虫标本馆。     

河南新(虫责)属(襀翅目,(虫责)科)一新种记述

记述采自河南的1新种,河南新Neoperla henana sp.nov.,并讨论了其与近似种的区别。模式标本保存在河南科技学院标本馆。河南新,新种Neoperla henana sp.nov.(图1～5)新种腹部第7背板隆突和第8背板的骨化突特征与师周新Neoperla magisterchoui Du,2000近似。二者的主要区别在于:新种雄虫阳茎囊略长于阳茎管,阳茎管端部有1对膜质突起。而师周新雄虫阳茎囊长度约为阳茎管的两倍,阳茎管端部有两对膜质突起。正模♂,河南南阳老界岭,2008-07-02,李卫海采,副模1♂,同正模。词源:新种以标本的模式产地命名。     

贵州新虫责属二新种记述1(襀翅目:虫责科:虫责亚科)

记述采自中国贵州的新虫责属 2新种 ,即双瘤新虫责 Neoperla bituberculata,sp.nov.和曲囊新虫责 N eoperla flexiscrotata,sp.nov.。模式标本存扬州大学植物保护系昆虫标本室 ( YU)     

(虫+责)科昆虫地理分布研究初探

本文就(虫+责)科昆虫的起源，各亚科、族及属的分布特点进行了分析和总结；并根据各属的世界分布情况，将其分为8种类型。文中还就中国(虫+责)科昆虫的地理分布及特点作了初步的探讨，结果表明：在我国已知的2亚科4族20个属中，东洋区分布的有10个属，其中有3个属仅分布于我国；古北区分布的有1个属；东洋-古北区分布的有3个属；东洋-新北区分布的有1个属；东洋-古北-新北区分布的有4个属；东洋-古北-新北-非洲区分布的有1个属。此外，(虫+责)科昆虫在中国的一个主要分布特点就是多数种类集中分布于华中区、华南区和西南区；而华中区则很可能是(虫+责)科种类的分化中心，并以此为中心向其他区扩散。     

七种蒿属植物种子重量形状及萌发特性的比较研究

在实验室条件下 ,对 7种蒿属植物种子 (差巴嘎蒿、乌丹蒿、万年蒿、大籽蒿、黄蒿、野艾蒿和冷蒿 )进行重量、形状及萌发特性的比较研究。沙生先锋植物乌丹蒿和差巴嘎蒿的种子重量较大、形状扁平 ,这些特征是植物对流沙环境进化的适应机制之一。黄蒿种子小且呈圆形 ,具有持久土壤种子库 ,因此黄蒿抗干扰能力较强。 7种蒿属植物有 3种萌发格局 :大籽蒿、万年蒿、差巴嘎蒿和冷蒿的萌发前期快 ,后期平缓 ;野艾蒿和黄蒿整个萌发过程平缓 ;乌丹蒿早期和后期萌发平缓 ,中间快。乌丹蒿推迟萌发高峰是它比差巴嘎蒿更适应流沙环境的机制之一。从种子萌发格局分析 ,黄蒿种子具有生理后熟或休眠机制 ,大籽蒿种子萌发是典型的机会主义。黄蒿、野艾蒿和冷蒿种子具有风险分摊的萌发机制。种子重量和形状与发芽率之间无相关性 ,重量和形状则显著相关。     

Substitution of methionine 63 or 83 in S100A9 and cysteine 42 in S100A8 abrogate the antifungal activities of S100A8/A9: potential role for oxidative regulation

S100A8 and S100A9 and their heterocomplex calprotectin (S100A8/A9) are abundant cytosolic constituents in human neutrophils previously shown to possess antifungal activity. This study was designed to investigate mechanisms involved in the modulation of the antifungal properties of S100A8/A9. S100A8, S100A9 and site-directed mutants of both proteins were tested for their antifungal effect against Candida albicans in microplate dilution assays. Whereas S100A8 alone did not inhibit fungal growth, S100A9 by itself had a moderate antifungal effect. Combining both proteins had the strongest effect. Supporting a potential role for oxidation in S100A8/A9, substitution of methionine 63 or 83 of S100A9 resulted in the loss of antifungal activity. Additionally, the substitution to alanine of cysteine 42 of S100A8 also caused a loss of S100A8's ability to enhance S100A9's antifungal effect. Overall, our data indicate that both S100A8 and S100A9 are required for their fully active antifungal effect and that oxidation regulates S100A8/A9 antifungal activity through mechanisms that remain to be elucidated and evaluated. Finally, together with our previous work describing the oxidation-sensitive anti-inflammatory effects of S100A8/A9, we propose that S100A8/A9 exerts an anti-inflammatory activity in healthy state and that conditions associated with oxidative stress activate the antifungal activity of S100A8/A9.     

In rat hepatocytes, different adenosine receptor subtypes use different secondary messengers to increase the rate of ureagenesis

In rat hepatocytes, the role of cAMP and Ca(2+) as secondary messengers in the ureagenic response to stimulation of specific adenosine receptor subtypes was explored. Analyzed receptor subtypes were: A(1), A(2A), A(2B) and A(3). Each receptor subtype was stimulated with a specific agonist while blocking all other receptor subtypes with a battery of specific antagonists. For the A(1) and A(3) adenosine receptor subtypes, the secondary messenger was the cytoplasmic Ca(2+) concentration ([Ca(2+)](cyt)). Accordingly, the A(1) or A(3)-mediated increase in [Ca(2+)](cyt) and in ureagenic activity were both inhibited by chelating Ca(2+) with either EGTA or BAPTA-AM. Also, Gd(3+) blocked both the increase in [Ca(2+)](cyt) and ureagenesis, suggesting that a Ca(2+) channel may be involved in the response to both A(1) and A(3). A partial effect was observed with the sarcoplasmic reticulum Ca(2+)-ATPase inhibitor thapsigargin. The concentration of cyclic AMP ([cAMP]) increased in response to stimulation of either the A(2A) or the A(2B) adenosine receptor subtypes, while it decreased slightly in response to stimulation of either A(1) or A(3). The stimulation of either the A(2A) or A(2B) adenosine receptor subtypes resulted in an increase in [cAMP] and an ureagenic response which were not sensitive to EGTA, BAPTA-AM, Gd(3+) or to thapsigargin. In addition, the adenylyl cyclase inhibitor MDL12,330A blocked the ureagenic response to A(2A) and A(2B), but not the response to either A(1) or A(3). Our results indicate that in the ureagenic liver response to adenosine, the secondary messenger for both, the A(1) and A(3) adenosine receptor subtypes is [Ca(2+)](cyt), while the message from the A(2A) and A(2B) adenosine receptor subtypes is relayed by [cAMP].     

Phylogenetic analysis of the genus Aquaspirillum based on 16S rRNA gene sequences

Phylogenetic analysis of 15 species of the genus Aquaspirillum based on 16S rRNA gene (rDNA) sequences indicated that the genus Aquaspirillum is phylogenetically heterogeneous and the species could be divided into four groups as follows: Aquaspirillum serpens, the type species of this genus, A. dispar and A. putridiconchylium are situated in the family Neisseriaceae; members of the second group, A. gracile, A. delicatum, A. anulus, A. giesbergeri, A. sinuosum, A. metamorphum and A. psychrophilum, are included in the family Comamonadaceae; the two members of the third group, A. arcticum and A. autotrophicum, are included in the family Oxalobacteriaceae; and members of the fourth group, A. polymorphum, A. peregrinum, and A. itersonii, are included in the alpha-subdivision of Proteobacteria. Thus, phylogenetic studies indicated that all the species excepting A. serpens, the type species, should be transferred to distinct genera.     

Adenosine A2A receptor is involved in cell surface expression of A2B receptor

The A2A and A2B adenosine receptors (A2AR and A2BR) are implicated in many physiological processes. However, the mechanisms of their intracellular maturation and trafficking are poorly understood. In comparative studies of A2AR versus A2BR expression in transfected cells, we noticed that the levels of cell surface expression of A2BR were significantly lower than those of A2AR. A large portion of the A2BR was degraded by the proteasome. Studies of cell surface expression of A2BR chimeric molecules in transfectants suggested that A2BR does not have the dominant forward transport signal for export from the endoplasmic reticulum to the cell surface. A2BR surface expression was increased in A2BR chimeras where the A2BR carboxyl terminus (CT) was replaced or fused with the A2AR CT. Co-transfection of A2AR with A2BR enhanced surface expression of A2BR though the F(X)(6)LL motif in the A2AR CT. The requirements of A2AR expression for better A2BR cell surface expression was not only established in transfectants but also confirmed by observations of much lower levels of A2BR-induced intracellular cAMP accumulation in response to A2BR-activating ligand in splenocytes from A2AR(-/-) mice than in wild type mice. The results of mechanistic studies suggested that poor A2BR expression at the cell surface might be accounted for mainly by the lack of a dominant forward transport signal from the endoplasmic reticulum to the plasma membrane; it is likely that A2BR forms a hetero-oligomer complex for better function.     

Contribution of conserved polar glutamine, asparagine and threonine residues and glycosylation to agonist action at human P2X1 receptors for ATP

The role of conserved polar glutamine, asparagine and threonine residues in the large extracellular loop, and glycosylation, to agonist action at human P2X1 receptors was tested by generating alanine substitution mutants. For the majority of mutants (Q56A, Q95A, T104A, T109A, Q112A, Q114A, T146A, N153A, T158A, N184A, N191A, N242A, N300A) alanine substitution had no effect on ATP potency. The mutants Q95A, Q112A, Q114A and T158A showed changes in efficacy for the partial agonists BzATP and Ap5A, suggesting that these polar residues may contribute to the gating of the channel. The mutants T186A, N204A and N290A had six-, three- and 60-fold decreases in ATP potency, respectively. For T186A and N290A, the partial agonists BzATP and Ap5A were no longer agonists but still bind to the receptor as shown by the ability to modulate the response to co-applied ATP. N153, N184 and N242 are glycosylated in the endoplasmic reticulum and N300 acquires complex glycosylation in the golgi. These results aid in refining a model for ATP binding at the P2X1 receptor where the residues F185T186, and the conserved triplet N290F291R292, are likely to play a role in ATP action at the receptor.     

中南半岛紫金牛科植物志预报

紫金牛科是一个典型的热带分布科,中南半岛种类非常丰富。Ｐｉｔａｒｄ（１９３０）在“Ｆｌｏｒｅ Ｇｅｎｅｒａｌｅ ｄｅ Ｌ＇Ｉｎｄｏｃｈｉｎｅ”中记录了６属１０９种。此后,对这一地区的种类无人作过全面深入的研究。最近作者在编研《柬埔寨、老挝和越南植物志》的过程中,对紫金牛科作了全面的修订,被确认的种类增加到７属１４２种,其中包括紫金牛属,酸藤子属和杜茎山属的３０个新种,１０个新变种,另外还有紫金牛属     

Neuroprotection by adenosine A2A receptor blockade in experimental models of Parkinson's disease

Adenosine A2A receptors are abundant in the caudate-putamen and involved in the motor control in several species. In MPTP-treated monkeys, A2A receptor-blockade with an antagonist alleviates parkinsonian symptoms without provoking dyskinesia, suggesting this receptor may offer a new target for the antisymptomatic therapy of Parkinson's disease. In the present study, a significant neuroprotective effect of A2A receptor antagonists is shown in experimental models of Parkinson's disease. Oral administration of A2A receptor antagonists protected against the loss of nigral dopaminergic neuronal cells induced by 6-hydroxydopamine in rats. A2A antagonists also prevented the functional loss of dopaminergic nerve terminals in the striatum and the ensuing gliosis caused by MPTP in mice. The neuroprotective property of A2A receptor antagonists may be exerted by altering the packaging of these neurotoxins into vesicles, thus reducing their effective intracellular concentration. We therefore conclude that the adenosine A2A receptor may provide a novel target for the long-term medication of Parkinson's disease, because blockade of this receptor exerts both acutely antisymptomatic and chronically neuroprotective activities.