共查询到18条相似文献,搜索用时 99 毫秒
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大果良种沙棘愈伤组织诱导及植株再生的研究 总被引:18,自引:1,他引:17
大果良种沙棘的幼嫩茎尖,茎段外植体接种在MS,1/2MS附加不同浓度配比的IAA,IBA,BA,NAA培养基上可诱导茎尖及腋芽生长,将诱导产生的无性系芽接种在MS或1/2MS附加BA0.3-0.5mg/L,NAA0.05mg/L的培养基上可形成丛生芽,同时在小叶片和嫩茎上诱导产生愈伤组织,继续培养愈伤组织表面形成大量的绿色突起,进一步分化成不定芽,在相同培养基上,不定芽上可直接产生不定芽,从而形成多达数百个的不定芽族,不定芽长至3cm时切下转至1/2MS附加IAA或IBA 0.2mg/L的培养基上可生根而形成完整 的再生植株。 相似文献
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香水白掌的组织培养和快速繁殖 总被引:4,自引:0,他引:4
1植物名称白鹤芋属品种香水白掌(Spathiphyllum“Xiangshui”)。2材料类别茎段、茎尖。3培养条件以MS为基本培养基。诱导丛生芽培养基:(1)MS+6-BA4~6mg·L-1(单位下同)+NAA0.2。丛生芽增殖培养基:(2)MS+6-BA2~4+NAA0.2;(3)培养基(2)+KH2PO485。4生长与分化情况4.1丛生芽的诱导取香水白掌茎段,剥去外层叶片,75%酒精消毒lmin,然后用灭菌净浸泡20~35min,切取茎尖和茎段,置于培养基(1)上培养。7d后开始出现侧芽萌发和茎尖萌发成苗。30d后将萌发苗基部切割,置于培养基(1)继续培养,2周后出现了… 相似文献
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魔芋属植物愈伤组织的诱导和再生植株的研究 总被引:10,自引:0,他引:10
用云南魔芋(Amorphophallus yunnanensis)、白魔芋(A.albus)、花魔芋(A.revieri)和勐海魔芋(A.bannaensis)等四个种的叶、鳞叶、花序、匍匐茎、块茎和茎尖为外植体,诱导产生小植物。可通过三种途径获得魔芋再生植株:(1)由外植体诱导愈伤组织,再分化小植株:在添加0.5—1.0mg/1 NAA和BA或KT、ZT的MS培养基上,高频率地诱导瘤状愈伤组织形成,发现NAA对愈伤组织的诱导是必不可少的,而细胞分裂素与之组合,促进瘤状愈伤组织的形成和发育,在无激素或低浓度激素的培养基上,瘤状愈伤组织分化出小植物;(2)茎尖和鳞叶、块茎切块培养,诱导形成多芽苗,产生再生植株;(3)块茎切块诱导产生微型小块茎,然后分化芽和根,长成小植物。本研究为魔芋的快速繁殖提供了新的手段。 相似文献
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In order to further increase shoot regeneration frequency of Vigna mungo (L.) Hepper., the effects of AgNO3 on this process was investigated in this study. The shoot tip and cotyledonary node explants were cultured on MS salts B5 Vitamins medium containing BA+TDZ+Ads+AgNO3 for multiple shoot induction. AgNO3 influenced the shoot bud formation and their subsequent proliferation. The best medium composition for multiple shoot induction was BA, TDZ combination with Ads and AgNO3 in MSB5 medium. Maximum 39 shoots in cotyledonary node and 22 shoots in shoot tip were obtained per explants after 4 – 6 wk. of culture. Elongation and rooting were performed in GA3 (0.6mg/l) and IBA (0.4mg/L) containing media respectively. The in vitro raised plantlets were acclimatized in green house and successfully transplanted to the field with a survival rate of 78%. 相似文献
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罗汉果组织培养中愈伤组织和腋生枝的形成 总被引:4,自引:0,他引:4
用罗汉果茎段为外植体,培养在MS+BA1.0+IBA0.1毫克/升培养基上,诱导愈伤组织和芽形成。观察了罗汉果茎段愈伤组织的生长以及腋生枝的形成。罗汉果茎段培养5天后,潜伏腋芽开始萌动和生长。培养10天后罗汉果茎段的基部一端开始膨大。培养20天后产生大量白色疏松的愈伤组织,这时腋生枝已经长成3—6厘米长。培养30天后基部的愈伤组织中有少量瘤状小突起,但再分化形成芽的频率极低。结果表明,罗汉果茎段组培形成的苗均是从腋芽产生的。 相似文献
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K. Sankara Rao 《Plant cell reports》1986,5(3):199-201
Callus-mediated shoot bud formation was demonstrated in Dalbergia latifolia Roxb. (East Indian Rosewood). Cultures were raised from shoot explants of six year-old plants on Murashige and Skoog (MS) medium supplemented with naphthaleneacetic acid (NAA) and benzyladenine (BA). A sequential treatment of callus with increasing BA levels and decreasing NAA ensured shoot bud induction. Rooting of shoots was achieved by a three-step culture procedure involving 1) White's(W) liquid medium containing indoleacetic acid (IAA), naphthaleneacetic acid and indolebutyric acid (IBA), 2) half-strength MS agar-solidified medium with charcoal (0.25%) and 3) half-strength MS liquid medium.Abbreviations BA
Benzyladenine
- IAA
Indoleacetic acid
- IBA
Indolebutyric acid
- MS
Murashige and Skoog
- NAA
a-naphthaleneacetic acid
- PVP
Polyvinylpyrrolidone
- W
White's medium
- 2,4-D
2,4-dichlorophenoxyacetic acid 相似文献
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Protocorm like bodies (PLBs), callus and shoot buds developed in culture from in vitro raised foliar explants of Cleisostoma racimeferum. Among the different basal media, better result was obtained on MS medium containing sucrose (3%) and BA (2 microM) with approximately 80% frequency after 40 days of culture. Young leaves (15 week old) produced better PLBs. Whole leaf placed vertically upside-up orientation can regenerate PLBs and shoot buds (80%). PLBs and shoot buds formed on entire surface of the leaves. Cultures on BA and NAA (2 and 2 microM respectively in combination) stimulated callus mediated regeneration (68%). The rooted plantlets regenerated within 8-10 week from PLBs and shoot buds on MS medium containing IAA and kinetin (2 microM each in combination). BA containing medium triggered multiple shoot bud formation, while NAA alone or in combination with other growth regulators was inhibitory. Incorporation of activated charcoal (0.01%) in the medium stimulated formation of repetitive PLBs and multiple shoot buds. Rooted plants were ready for harvest after 20-22 week of initiation of culture. About 65% of the potted plants survived after 3 months in the poly house. 相似文献
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Kosturkova Georgina Mehandjiev Atanas Dobreva Irina Tzvetkova Veska 《Plant Cell, Tissue and Organ Culture》1997,48(2):139-142
Ten genotypes from Pisum sativum and Pisum arvense were screened for their regeneration abilities. Most of them were created
through experimental mutagenesis from Bulgarian varieties and have various valuable agronomic traits. Embryonic axes from
immature embryos were plated on modified Murashige and Skoog medium, containing different concentrations of 2,4-dichlorophenoxyacetic
acid (2,4-d), α-naphthaleneacetic acid (NAA) and benzyladenine (BA). Two schemes for direct and indirect organogenesis were
established. Callus and shoot formation were induced on media containing 0.2 mM 2,4-d or 5 mM BA, respectively. Embryonic
axes formed buds directly when plated on medium with 10 mM BA and 1 mM NAA. Organogenesis and adventitious bud formation were
maintained on medium supplemented with BA and NAA. Rhizogenesis was induced on Gamborgs' B5 medium. All screened genotypes
were able to regenerate plants with a high efficiency (50–100%) although some differences in their organogenetic response
were observed.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
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Bodhipadma Kitti Noichinda Sompoch Padyencheun Winan Khunthacharoen Theerapong Chikhunthod Utorn Leung David W. M. 《Plant Cell, Tissue and Organ Culture》2011,105(3):465-469
The shoots developed from both the shoot tip and nodal explants of feathered amaranth (Celosia argentea var. plumosa—feathered cockscomb or plumed cockscomb) after 8 weeks of culture in the presence of either paclobutrazol or benzyladenine
(BA) were shorter than those developed on basal Murashige and Skoog (MS) medium (Physiol Plant, 15:473–497, 1962) alone. However, this retarding effect was more pronounced in the nodal explant culture. Shoot tip explants from 2-week-old
seedlings were more adversely affected by 0.85 or 1.7 μM paclobutrazol than those from older seedlings. In contrast, regardless
of preculture duration investigated nodal explants did not exhibit different response to three different concentrations of
paclobutazol. The response to 2.2 or 4.4 μM BA appeared to be largely independent of the age of the shoot tip explants or
preculture treatment of nodal explants. Shoots developed from nodal explants produced a higher number of terminal inflorescence
than those from shoot tip explants. Moreover, only lateral shoots from nodal explant culture formed inflorescence. Increased
preculture duration on basal MS medium could generally lessen the inhibitory effect of lower concentrations of paclobutazol
or BA on terminal or lateral inflorescence formation in nodal explant culture. 相似文献
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A highly efficient two stage protocol was developed for induction of multiple shoots from single node in vitro shoot tip explants of Decalepis hamiltonii. It was found that phloroglucinol (PG) had synergistic effect on shoot multiplication when added with N6-benzyladenine and gibberellic acid. This protocol uses PG for both multiple shoot induction from nodal explants, elongation of primary shoots and initiation of adventitious shoot formation from primary shoots, which was more in presence of triacontanol (TRIA). Maximum number of shoots per culture was observed on the medium containing N6-benzyladenine (1.1 microM; BA), GA3 (5.8 microM) and PG (800 microM). Sub-culturing of the shoots onto MS medium containing optimum concentration of BA (5.6 microM), PG (200 microM) and TRIA (0.011 microM) produced elongated shoots along with secondary shoot formation. The long shoots were rooted on alpha-naphthalene acetic acid (5.38 microM; NAA) and PG (400 microM) containing medium. The rooted plantlets were hardened and their field survival rate was 80-90%. 相似文献
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Conditions for plant regeneration from excised shoot tips of Vigna radiata were studied. Complete plants were regenerated directly without an intervening callus phase from shoot tips on basal medium (MS salts+B5vitamins). Regeneration frequency varied with genotype, explant size and growth regulator combinations in the medium. Addition of cytokinins induced a variable amount of callus at the base of the shoot tip, followed by multiple shoot formation. Benzyladenine (BA), kinetin and zeatin at 5×10-6 M each induced multiple shoots in 100% of the explants but the highest number of regenerants per explant (9) was produced with BA. The efficacy of BA for shoot multiplication was not improved when it was supplemented with naphthaleneacetic acid (NAA) or indoleacetic acid (IAA). NAA or adenine sulphate, when applied alone, induced complete plantlets. The growth regulator requirement of explants for the induction of multiple shoots varied with explant size. The shoot tip explants maintained proliferation ability on subculture. None of the treatments was effective in inducing shoot bud differentiation from callus. Regenerated shoots were rooted on MS basal medium and MS supplemented with either IAA or indolebutyric acid. The rooted plants were transferred to the field; 60% subsequently survived and grew.Abbreviations BM
basal medium [MS (Murashige & Skoog 1962) salts+B5 (Gamborg et al. 1968) vitamins]
- BA
6-benzyladenine
- AdS
adenine sulphate
- IAA
indole-3-acetic acid
- NAA-1
naphthaleneacetic acid
- IBA
indolebutyric acid 相似文献