短角湿生冷水花化学成分的研究

为研究短角湿生冷水花(Pilea aquarum subsp.brevicornuta)化学成分。实验采用色谱法从中分离得到7个化合物,包括5个五环三萜、一个甾醇和一个木脂素。利用波谱学方法鉴定了它们的结构,分别鉴定为pilearbornol(1)、rubiarbonone D(2)、camarolide(3)、表齐墩果酸(4)、齐墩果酮酸(5)、5,8-epidioxy-(3β,5α,8α,22E)-ergosta-6,9(11),22-trien-3-ol(6)和桉脂素(7)。其中,化合物1和2属于乔木萜烷型(arborane)三萜,化合物1为新化合物,化合物2的绝对构型首次通过X-射线单晶衍射得到了确定。所有化合物均为首次从该植物中分离得到,这也是首次从荨麻科植物中分离得到乔木萜烷型三萜化合物。     

海芒果叶中三萜类成分的研究

采用多种柱色谱等分离纯化手段对海芒果叶中的三萜类成分进行分离,得到7个化合物,通过理化性质和波谱方法鉴定化合物为熊果醇(1),(23Z)-9,19-cycloart-25-ene-3β,24-diol(2),大戟醇(3),乌苏酸(4),2α-羟基乌苏酸(5),乙酰乌苏酸(6),α-香树脂醇(7)。化合物1～6均为首次从该属植物中分离得到。     

川赤芍的化学成分研究

从川赤芍Paeonia anomala subsp. veitchii 根皮的70%丙酮提取物中, 分离鉴定了22 个化合物, 其中包括一个新的24 , 30 位降常春藤皂苷三萜衍生物, 命名为paeonenolide H ( 1) 。化合物2 , 4 , 9 , 10 为首次从该植物中分离得到。     

开口箭根茎中甾体化合物的研究

从开口箭根茎部位共分离得到9个甾体化合物,经波谱数据分析结合文献对照分别鉴定为3-epi-neoruscogenin-3-β-D-glucopyranoside(1),3-epi-ruscogenin-3-β-D-glucopyranoside(2),ranmogenin A(3),convallagenin B(4),3-epi-neoruscogenin(5),tupichigenin E(6),(20S,22R)-spirost-25(27)-ene-1β,2β,3β,4β,5β,7α-hexaol-6-one(7),β-谷甾醇(8)和胡萝卜苷(9)。其中化合物1、2和4为首次从该植物中分离得到。     

黄檗落叶化学成分的研究

从黄檗落叶的95％乙醇提取物中分离鉴定了7个化合物,分别为phellodenol-A(1),茵芋苷(skimmin,2),phellodenol E(3),黄檗苷(amurensin,4),黄柏苷(phellamurin,5),(2R) -phellodensin-F(6)和clerosterol 3-Oβ-D-galactopyranoside (7).其中,化合物7为首次从黄柏属植物中分离得到,化合物3和6为首次从该植物中分离得到.     

川赤芍的化学成分研究

从川赤芍Paeonia anomala subsp·veitchii根皮的70%丙酮提取物中,分离鉴定了22个化合物,其中包括一个新的24,30位降常春藤皂苷三萜衍生物,命名为paeonenolide H(1)。化合物2,4,9,10为首次从该植物中分离得到。     

绿茎还阳参化学成分的研究

采用柱层析和重结晶从药用植物绿茎还阳参(Crepis lignea)丙酮提取物中分离得到7个单体化合物,并根据化合物的理化性质和波谱分析,分别鉴定为:3β-acetoxyurs-13( 18)-ene (1),lupeol acetate (2),4-hydroxy-3 -methoxycinnamaldehyde (3),(Z)-2-ethylidene-3-methylsuccinic acid (4),olean- 12 -ene-11 α-methoxy-3β-acetate (5),α-amyrin acetate (6)和lupeol-9( 11) en-3β-acetate(7).上述化合物均为首次从绿茎还阳参中分离得到.     

毛剪秋罗的化学成分研究

从毛剪秋罗(Lychnis coronaria(L.)Desr.)全草的乙醇提取物中分离鉴定了11个已知化合物,经波谱分析确定其结构分别为tricin 7-O-glucopyranoside(1),(+)-isoscoparin(2),epoxyactinidionoside(3), 1α, 20R- hydroxyecdysone ( 4 ), ecdysterone ( 5 ), polypoding B ( 6 ), ecdysterone 22-O-β-D-glucopyranoside(7), stigmast-5-ene-3-one(8), taraxerol ( 9 ), a-tocopherol (10), dehydrodiconiferyl alcohol 4-O-β-D-gluoopyranoside(11).此外,还分离得到β-谷甾醇和胡萝卜苷.以上化合物均为首次从该植物中分离得到.     

中国广西红树林植物海漆的化学成分研究

用硅胶柱层析和凝胶柱层析对红树林植物海漆(Excoecaria agallochaL.)的乙酸乙酯提取物进行分离纯化,从中分离得到9个化合物;通过波谱学数据它们分别鉴定为kayadiol(1)、8(14),15-isopimaradiene-7α,18-diol(2)5、,11-epoxy-9-hydroxy-7-megastigmen-3-one(3)、annuionone E(4)、28,30-dihydroxy-lup-20(29)-ene-3-one(5)、mes-sagenic acid G(6)、3,β24-dihydroxy-12-oleanen-28-oic acid(7)s、yringaresinol(8)、ethyl gallate(9)。这些化合物均为首次从该植物中分离得到。     

菜蕨的化学成分研究

采用硅胶柱层析和凝胶柱层析对菜蕨的丙酮提取物进行分离纯化,首次从中分离得到10个化合物,通过波谱学数据并和已知化合物数据比较,鉴定它们分别为β-sitosterol(1),Stigmast-4-ene-6β-ol-3-one(2),Stigmast-4-ene-3,6-dione(3),Benzeneacetic acid(4),3β-Hydroxy-5α,8α-epidioxyergosta-6,22-diene(5),Stigraast-4-ene-3β,6β-diol(6),Stigmast-5-ene-3β,7α-diol(7),Stigmast-4-ene-6α-ol-3-one(8),Glycerol-1,3-dihexadecanoate(9)以及Daucosterol(10).     

Tyrosinase inhibitory cycloartane type triterpenoids from the methanol extract of the whole plant of Amberboa ramosa Jafri and their structure-activity relationship

New tyrosinase inhibitory cycloartane triterpenoids have been discovered from the methanol extract of the whole plant of Amberboa ramosa (Roxb.) Jafri, which is a member from the Compositae family. Utilizing the conventional spectroscopic techniques, including 1D and 2D NMR analysis, and also by comparing the experimental with literature data, the isolated compounds proved to be cycloartane type triterpenoids. These cycloartanes are: (22R)-cycloart-20, 25-dien-2alpha3beta22alpha triol (1), (22R)-cycloart-23-ene-3beta, 22alpha, 25-triol (2), cycloartenol (3), cycloart-23-ene-3beta, 25-diol (4), cycloart-20-ene-3beta, 25-diol (5), cycloart-25-ene-3beta, (22R) 22-diol (6), 3beta, 21, 22, 23-tetrahydroxy-cycloart-24 (31), 25 (26)-diene (7), and (23R)-5alpha-cycloart-24-ene-3beta, 21, 23-triol (8). Out of these eight compounds, compound 3 did not show any activity against the enzyme tyrosinase. Among them compound 7 was found to be the most potent (1.32 microM) when compared with the standard tyrosinase inhibitors kojic acid (16.67 microM) and L-mimosine (3.68 microM). Finally in this paper, we have discussed the structure-activity relationships of these molecules.     

粗枝崖摩中一个新的三萜(英文)

Five compounds were isolated from the EtOH extraction of the stem of Amoora dasyclada (How et T.Chen) C.Y.Wu (Meliaceae). On the basis of spectroscopic methods, their structures were elucidated as 24, 25-epoxy-tirucall-7-ene-3, 23-dione (1),24,25,26,27-tetranortirucall-7-ene-3-oxo-23(21)-Iactone (2),taraxerone (3),taraxerol (4)and β-sitosterol (5).Among them, compound 1 was a new triterpenoid, compounds 3-5 were firstly obtained from this plant; compound 2,an tetranortriterpenoid, was firstly isolated from natural sources, and its NMR data were assigned for the first time. Moreover, the △^7-bond and the Me-14 in compound 2 were never changed, which has never been found in other tetranortriterpenoids. And the biosynthetic pathway of tetranortriterpenoid was further discussed.     

二色补血草地上部分的化学成分

二色补血草﹝Limonium bicolor ( Bunge) Kuntze﹞为补血草属( Limonium Mill.)越年生草本植物,耐盐碱和抗逆性强,全草均可入药,具有补血止血、调经益气、抗菌消炎和温中健脾的作用[1],含有黄酮、多糖、挥发油和甾体等成分[2-7],其中,黄酮类成分具有较强的抗氧化能力,多糖对Hela细胞有明显抑制作用[8-11]。前人对二色补血草化学成分和药理活性已有一定研究,但对其地上部分的化学成分尚缺乏系统分析。为此,作者对二色补血草地上部分乙醇提取物进行了分离和鉴定,为该种的进一步开发利用和药理药效研究提供基础实验数据。     

酸渣树中一个新的黄烷类化合物

从酸渣树(Carapaguianensis Aubl.)枝条的乙醇提取物中首次分离到7个化合物,通过波谱方法鉴定,它们分别是:()-epicatechin-3-O-(3",5"-di-O-methyl)gallate(1)、(-)-catechin(2)、sciadopitysin(3)、cl eomi scosin B(4)、photogedunin(5)、chi socheton compound F(6)和odoratone(7).其中,化合物1为新黄烷类化合物,2～7为首次从该植物中分离得到.补充了化合物5的13C-NMR谱数据.活性测定显示,化合物7对大菜粉蝶(Pieris brassicae)三龄幼虫具有较强的杀虫活性,化合物2有一定的活性,而正丁醇部分只有很弱的活性.     

Two unsymmetric tetracyclic triterpenoids from cissus quadrangularis

Two new unsymmetric tetracyclic triterpenoids onocer-7-ene-3α,21β-diol and onocer-7-ene-3β,21α-diol together with sitosterol, δ-amyrin and δ-amyrone have been isolated from Cissus quadrangularis. The structures of the new compounds were elucidated on the basis of ¹H NMR, mass spectral and chemical evidence.     

Triterpenes of five euphorbiaceae species of Sri Lanka

Five endemic species of Euphorbiaceae have been investigated. Aleuritolic acid and its derivatives were isolated from P. thwaitesii. A. cardiosperma had friedelan derivatives. The Glochidion species contained the same triterpenes, viz. glochidonol, lup-20(29)-ene-3α,23-diol and glochidiol. The bark extractives of a new Glochidion species had, in addition, glochidone and probably lup-20(29)-ene-1α,3α,23-triol. The latter is a new natural product. Glochidone was isolated for the first time outside the genus Glochidion from Bridelia moonii.     

Triterpene saponins from Silphium radula

Nine triterpene saponins (1-9) were isolated from leaves and stems of Silphium radula Nutt. (Asteraceae). Their structures were determined by extensive 1D ((13)C, (1)H, DEPT, TOCSY) and 2D NMR (NOESY, HSQC, HMBC) and ESI-MS studies. The compounds were identified as 3beta,6beta,16beta-trihydroxyolean-12-en-23-al-3-O-beta-glucopyranosyl-16-O-beta-glucopyranoside (1), urs-12-ene-3beta,6beta,16beta-triol-3-O-beta-galactopyranosyl-(1-->2)-beta-glucopyranoside (2), 3beta,6beta,16beta-trihydroxyolean-12-en-23-oic acid-3-O-beta-glucopyranosyl-16-O-beta-glucopyranoside (3), urs-12-ene-3beta,6beta,16beta,21beta-tetraol-3-O-beta-glucopyranoside (4), olean-12-ene-3beta,6beta,16beta,21beta-tetraol-3-O-beta-glucopyranoside (5), olean-12-ene-3beta,6beta,16beta,21beta,23-pentaol-3-O-beta-glucopyranosyl-16-O-beta-glucopyranoside (6), olean-12-ene-3beta,6beta,16beta-triol-3-O-beta-glucopyranosyl-16-O-alpha-arabinopyranosyl-(1-->2)-beta-glucopyranoside (7), olean-12-ene-3beta,6beta,16beta,23-tetraol-3-O-beta-glucopyranosyl-16-O-alpha-arabinopyranosyl-(1-->2)-beta-glucopyranoside (8), 3beta,6beta,16beta,21beta-tetrahydroxyolean-12-en-23-al-3-O-beta-glucopyranoside (9). The presence of a 6beta-hydroxyl function was not common in the oleanene or ursene class and the aglycones of these compounds were not found previously in the literature. Moreover, the cytotoxic activities of the isolated compounds were tested against human breast cancer cell line MDA-MB-231. Results showed that compound 2 decreased cell proliferation in a statistically significant manner at 25 microg/ml.     

Structure and cytotoxicity of new polyhydroxylated sterols from the Caribbean gorgonian Plexaurella grisea.

The gorgonian Plexaurella grisea contains the new steroids 9-hydroxygorgosterol (1), 9,11 alpha,14-trihydroxygorgosterol (2), 5 beta,6 beta-epoxyergost-24(28)-ene-3 beta,7 beta-diol (3), ergost-24(28)-ene-3 beta,5 alpha,6 beta,7 beta-tetrol (4), an unseparable 1:1 mixture of the epimers (25R) and (25S)-26-acetoxy-3 beta,5 alpha-dihydroxyergost-24(28)-en-6-one (5/6), and seven related, known compounds (7-13). The structures of these new compounds were defined by spectroscopic analysis. All the compounds (1-13) isolated from P. grisea were tested against P 388, A 549, and HT 29 tumor cell lines. Compounds 3, 5/6, and 12 exhibited selective activity against the HT 29 cell line (ED(50) = 0.1 microg/ml).