Discovery of a Homolog of Siderophilin in a Plant

Members belonging to the siderophilin family are iron-binding and iron-transporting proteins, which includes transferrin and lactoferrin. They have only been found in animals previously. If siderophilin could be found in and isolated from a plant, its production and subsequent extensive application could be increased. The present study is the first to report the discovery of a homolog of siderophilin in a plant. In order to purify antifreeze proteins from Ammopiptanthus mongolicus （Maxim.） Cheng f., the authors processed the proteins from the leaves using techniques such as column chromatography using DEAE-Cellulose-52, gel filtration via Sephacryl S-100 HR medium, hydrophobic interaction chromatography, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Mass spectroscopy was performed on the three proteins purified and the sequence of one of the proteins （containing 32 amino acids） was found to have 97% homology with the corresponding part of one type of human lactoferrin. Moreover, one of the two peptides belongs to an iron-binding domain. So, it is possible that siderophilin also exists in plants and plays a role as an antibacterial and antifungal, among other actions.     

Dynamics of a mutualism in a multi-species context

Despite recent findings that mutualistic interactions between two species may be greatly affected by species external to the mutualism, the implications of such multi-species interactions for the population dynamics of the mutualists are virtually unexplored. In this paper, we ask how the mutualism between the shoot-base boring weevil Apion onopordi and the rust fungus Puccinia punctiformis is influenced by the dynamics of their shared host plant Cirsium arvense, and vice versa. In particular, we hypothesized that the distribution of the weevil's egg load between healthy and rust-infected thistles may regulate the abundance of the mutualists and their host plant. In contrast to our expectations we found that the dynamics of the mutualists are largely determined by the dynamics of their host. This is, to our knowledge, the first demonstration that the dynamics of a mutualism are driven by a third, non-mutualistic species.     

Decay instability of a laser pulse propagating in a transverse direction in a magnetized plasma

The decay instability of a lser pulse propagating across an external magnetic field in a subscritical plasma is investigated analytically and numerically. It is shown that, when the relaxation of the pulse is taken into account, the hydrodynamic growth rate of the decay instability is slower than that obtained earlier in the constant-amplitude pump wave approximation. The results of numerical simulations by a particle-in-cell method demonstrate that an increase in the amplitude of the parametrically excited waves is accompanied by a decrease in their group velocity; in this case, up to 85% of the laser energy is converted into the energy of the plasma particles. It is found that, under resonance conditions, the magnetic field acts to increase the energy of the accelerated ions that escape from the plasma slab through its front boundary.     

Evolution of a Langmuir wave in a weakly inhomogeneous plasma in a longitudinal electric field

The spatial evolution of a Langmuir wave excited by external sources in a weakly inhomogeneous electron plasma in a longitudinal electrostatic field is considered. It is shown that, in a longitudinal electrostatic field, a Langmuir wave can only be amplified in an inhomogeneous plasma provided that the current of trapped electrons exceeds that of untrapped electrons. In this case, as the wave propagates through the inhomogeneous region where its phase velocity increases, some untrapped electrons become trapped in the wave potential wells. As a result, the current of trapped electrons increases and the wave is amplified. Moreover, in the regions where the bulk electrons are localized, the minima of the wave are amplified to a greater extent than its maxima.     

Prostatectomy in a county hospital; a review of 677 cases in a six-year period

Transurethral procedures were used in 620 of 677 cases in which prostatectomy was done (principally by residents supervised by a urologist) at a county hospital in a six-year period. Open operations were used in the other 57 cases. Results were classified as "excellent" in 46.8 per cent of the transurethral cases and as "good" in 36.3 per cent.     

LCA of a tomato crop in a multi-tunnel greenhouse in Almeria

Purpose

Protected crops have expanded significantly in the Mediterranean area over the last few decades as a successful means to provide abundant and high-quality produce. Although resources are generally used efficiently, greenhouse areas cause major environmental impacts. The aim of this work was to study, from an environmental point of view, the improvement capacity of greenhouse areas in the Mediterranean region and to assess several alternative agricultural practices to decrease their contribution to the environmental impacts in this system.

Materials and methods

The methodology used was life cycle assessment (LCA) based on a tomato crop grown in a multi-tunnel greenhouse in Almeria, on the southeast coast of Spain. The functional unit chosen was 1 ton of loose classic tomatoes. Five midpoint impact categories and one energy flow indicator were selected for their relevance. The agricultural practice alternatives evaluated were reduction of volume of substrate and fertilizers, extension of substrate and greenhouse life span and increase in renewable energy for electricity production.

Results and discussion

The results indicated that the main contributors to impact categories in the tomato production were structure, auxiliary equipment and fertilizers. Structure accounted for between 30 and 48?% of the contributions, depending on the impact category. The principal burdens in the auxiliary equipment stage were substrate and consumption of electricity. Fertilizers environmental impacts were due to emissions during their manufacture and application to the crop. In a best-case option, taking into account the best alternatives, contributions to the impact categories were reduced by between 17 and 30?%. The LCA methodology proved to be a useful tool to evaluate the environmental damage of this agricultural activity. The importance of including farm infrastructure in the assessment was demonstrated as it was a major contributor. The risk of eutrophication could be reduced by adjustment of the fertilizers?Cwater balance and implementation of a closed-loop irrigation system. Future technological improvements should be developed to increase yields and thereby directly reduce the environmental burdens per unit produce.

Conclusions

The present study served to assess the environmental impacts of a tomato crop in a multi-tunnel greenhouse on the coast of Almeria. The assessment was used to evaluate alternatives for improvement of cleaner production in greenhouse areas. Further research should focus on assuring the feasibility of the suggested options.     

Dynamics of a vortex filament in a stratified medium

The behavior of a vortex filament in a perfectly conducting stratified medium is analyzed. It is shown that the equation describing oscillations of a straight filament is linear, but becomes substantially non-linear with increasing inclination angle. Effects related to the finite radius of the vortex core are considered, and dispersion relations for linear oscillations of a vortex column are derived.     

Helix-coil transitions of a polypeptide in a two-component solvent

A microscopic model is considered of a helix-coil transition of polypeptides in a two-component solvent one of whose components competes for the formation of hydrogen bonds with the peptide group. It is shown that by a redetermination of the temperature parameter the model is reduced to a polypeptide chain without the solvent. Behavior of the transition parameters is calculated in relation to the solvent concentration competing for the formation of hydrogen bonds at different parameters of polypeptide-solvent interaction.     

Correction of a Genetic Defect in a Mammalian Cell

Foreign genetic material, in amounts too small to determine the synthesis of foreign surface antigens, can be incorporated into somatic cells by a special application of the technique of cell fusion. The interpolated genetic material is expressed and replicated in the foreign environment.     

Detection of a Target Taste in a Complex Masker

Detection thresholds for sodium chloride were compared in aqueoussolution, in mixture with a sucrose masker, in mixture witha citric acid masker, and in mixture with both of these maskerstogether. Separately the two maskers raised the threshold ofsodium chloride by three to four times, and together by overnine times, a result consistent with independence (additivity)of the two masking effects. To achieve comparable masking witheither sucrose alone or with citric acid alone would requireincreasing their masking concentrations by about ten times.Hence multiple masking can be a far more efficient means ofconcealing a taste, whether an unpleasant one (e.g. the bittertaste of medicine) or a pleasant one (e.g. a salty or sweetcondiment). Multiple masking has dietary and culinary significance,especially for middle aged and elderly persons concerned aboutsalt intake, because their thresholds for NaCl, whether withor without maskers, are typically two or three times higherthan those of youthful persons. Chem. Senses 22: 529–534,1997.     

Successful invasion of a food web in a chemostat

A food web in a chemostat is considered in which an arbitrary number of competitor populations compete for a single, essential, nonreproducing, growth-limiting substrate, and an arbitrary number of predator populations prey on some or all of the competitor populations. Although any number of predator populations may prey on the same competitor population, each predator population preys on only one competitor population. The dynamics of substrate uptake is modeled by Lotka-Volterra or Michaelis-Menten (Holling type I or II), but the dynamics of competitor uptake is restricted to Lotka-Volterra. Based on certain parameters, the model predicts the asymptotic survival or extinction of each of the different populations and suggests how competitor and/or predator populations could successfully invade the chemostat with or without causing a diverse ecosystem to crash. Similarly, it suggests how the elimination of certain populations could result in a more diverse or less diverse system.     

Induced indirect defence in a lycaenid-ant association: the regulation of a resource in a mutualism

Indirect defences involve the protection of a host organism by a mutualistic partner. Threat of predation to the host organism may induce the production of rewards and/or signals that attract the mutualistic partner. In laboratory and field experiments we show that threatened lycaenid butterfly larvae (Plebejus acmon) produce more nectar rewards from their gland and were tended by protective ants twice as much as controls. Ant attendance did not affect the leaf consumption or feeding behaviour of larvae in the absence of predators. Inducible nectar production and indirect defence in this system may be a mechanism by which larvae provide rewards for services when they are needed the most. Such a system may stabilize the mutualistic association between lycaenid larvae and ants by preventing exploitation by either partner.     

Mechanisms of coexistence of a bacteria and a bacteriophage in a spatially homogeneous environment

When bacteriophage are added to laboratory bacteria populations, bacteria mutants that are resistant to the phage quickly dominate the population. The phage will only persist in the long‐term if there are sufficient bacteria in the population that show susceptibility to the phage. We investigated the mechanisms allowing for coexistence by adding the virulent bacteriophage φ6 to cultures of the bacterium Pseudomonas syringae pv. phaseolicola in a spatially homogeneous environment. We saw large differences between replicate cultures, in particular when one or both of the species persisted. These differences can be explained by variation in the timing of the appearance of various resistant phenotypes in the bacteria populations before the phage were added, which determines their relative frequencies within the populations. Although these resistant phenotypes have similar fitnesses in the presence and in the absence of the phage, they have a profound effect on the persistence of the phage. Our results give a clearer understanding of the ecological mechanisms that lead to the coexistence of bacteria and virulent phage in environments where there are no spatial refuges available to the bacteria population.