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1.
The taxonomic positions of five Gram-negative, non-spore-forming and non-motile bacterial strains isolated from the rhizosphere of sand dune plants were examined using a polyphasic approach. The analysis of the 16S rRNA gene sequence indicated that all of the isolates fell into four distinct phylogenetic clusters belonging to the genus Chryseobacterium of the family Flavobacteriaceae. The 16S rRNA gene sequence similarities of isolates to mostly related type strains of Chryseobacterium ranged from 97.5% to 98.5%. All strains contained MK-6 as the predominant menaquinone, and iso-C 15:0, iso-C 17:0 3-OH and a summed feature of iso-C 15:0 2-OH and/or C 16:1 ω7c as the dominant fatty acids. Combined phenotypic, genotypic and chemotaxonomic data supported that they represented four novel species in the genus Chryseobacterium, for which the names Chryseobacterium hagamense sp. nov. (type strain RHA2-9 T=KCTC 22545 T=NBRC 105253 T), Chryseobacterium elymi sp. nov. (type strain RHA3-1 T=KCTC 22547 T=NBRC 105251 T), Chryseobacterium lathyri sp. nov. (type strain RBA2-6 T=KCTC 22544 T=NBRC 105250 T), and Chryseobacterium rhizosphaerae sp. nov. (type strain RSB3-1 T=KCTC 22548 T=NBRC 105248 T) are proposed. 相似文献
2.
A Gram-reaction-negative bacterium, strain IMMIB L-12475 T, was isolated from blood cultures of a human with septicaemia. The yellowish orange pigmented strain contained flexirubin pigment. Phylogenetic analysis based on 16S rRNA gene sequence revealed that strain IMMIB L-12475 T belonged to the family Flavobacteriaceae, forming a distinct phyletic line that is distantly related (79.1–89.4% sequence similarity) to described genera of this family. Membership to the family was confirmed by a fatty acid profile consisting of branched-chain and 3-hydroxy fatty acids with major amounts of iso-C 17:0 3-OH and iso-C 15:0, by the presence of menaquinone MK-6 as the only respiratory quinone and a polyamine pattern that contained sym-homospermidine as major component. The phospholipids consisted of phosphatidylethanolamine and an unknown phospholipid. The genomic DNA mol% G+C content was 45.6%. The distant phylogenetic position as compared to other representative of the family and the significant phenotypic properties such as pigment composition, morphology, and physiology support the proposal of a novel genus and species Cruoricaptor ignavus gen. nov., sp. nov. The type strain is IMMIB L-12475 T (=DSM 25479 T = CCUG 62025 T). 相似文献
3.
Two bacterial strains, KIS66-7 T and 5GH26-15 T, were isolated from soil samples collected in the South Korean cities of Tongyong and Gongju, respectively. Both strains were aerobic, Gram-stain-positive, mesophilic, flagellated, and rodshaped. A phylogenetic analysis revealed that both strains belonged to the family Microbacteriaceae of the phylum Actinobacteria. The 16S rRNA gene sequence of strain KIS66-7 T had the highest similarities with those of Labedella gwakjiensis KSW2-17 T (97.3%), Cryobacterium psychrophilum DSM 4854T (97.2%), Leifsonia lichenia 2Sb T (97.2%), Leifsonia naganoensis JCM 10592 T (97.0%), and Cryobacterium mesophilum MSL-15 T (97.0%). Strain 5GH26-15 T showed the highest sequence similarities with Leifsonia psychrotolerans LI1T (97.4%) and Schumannella luteola KHIAT (97.1%). The 16S rRNA gene sequence from KIS66-7 T exhibited 96.4% similarity with that from 5GH26-15 T. Strain KIS66-7 T contained a B2γ type peptidoglycan structure with D-DAB as the diamino acid; MK-13, MK-12, and MK-14 as the respiratory quinones; ai-C 15:0, ai-C 17:0, and i-C 16:0 as the major cellular fatty acids; and diphosphatidylglycerol, phatidylglycerol, and glycolipids as the predominant polar lipids. Strain 5GH26-15T had a B2β type peptidoglycan structure with D-DAB as the diamino acid; MK-14 and MK-13 as the respiratory quinones; ai-C 15:0, i-C 16:0, and ai-C{vn17:0} as the major cellular fatty acids; and diphosphatidylglycerol, phatidylglycerol, and glycolipids as the predominant polar lipids. Both strains had low DNA-DNA hybridization values (<40%) with closely related taxa. Based on our polyphasic taxonomic characterization, we propose that strains KIS66-7 T and 5GH26-15 T represent novel genera and species, for which we propose the names Diaminobutyricibacter tongyongensis gen. nov., sp. nov. (type strain KIS66-7 T=KACC 15515 T=NBRC 108724 T) and Homoserinibacter gongjuensis gen. nov., sp. nov. (type strain 5GH26-15 T=KACC 15524 T=NBRC 108755 T) within the family Microbacteriaceae. 相似文献
4.
A thermophilic bacterium, designated strain SYSU G04325T, was isolated from a hot spring sediment in Yunnan, China. Polyphasic taxonomic analyses and whole-genome sequencing were used to determine the taxonomic position of the strain. Phylogenetic analysis using 16S rRNA gene sequences indicated that strain SYSU G04325T shows high sequence similarity to Thermoflexibacter ruber NBRC 16677T (86.2%). The strain can be differentiated from other species of the family Thermoflexibacteraceae by its distinct phenotypic and genotypic characteristics. Cells of the strain SYSU G04325T were observed to be aerobic, Gram-stain negative and filamentous. Growth was found to occur optimally at 45 ºC and pH 7.0. In addition, the respiratory quinone was identified as menaquinone-7, while the major fatty acids (>?10%) were identified as iso-C15:0, iso-C17:0 and Summed Feature 9 (iso-C17:1ω9c). The polar lipids detected included phosphatidylethanolamine, three unidentified phospholipids, one unidentified glycolipid, five unidentified aminolipids and four unidentified polar lipids. The G?+?C content of the genomic DNA was determined to be 47.6% based on the draft genome sequence. On the basis of phenotypic, genotypic and phylogenetic data, strain SYSU G04325T is concluded to represent a novel species of a novel genus in the family Thermoflexibacteraceae, for which the name Rhodoflexus caldus gen. nov., sp. nov. is proposed. The type strain of Rhodoflexus caldus is SYSU G04325T (=?MCCC 1K06127T?=?KCTC 82848T). 相似文献
5.
Strain 268506 T was isolated from a root of Avicennia marina collected at mangrove forest in Wengchang, Hainan province, China. The 16S rRNA gene sequence of strain 268506 T showed the highest similarity with Micromonospora equina Y22 T (98.8 %) and Micromonospora olivasterospora DSM 43868 T (98.7 %). In addition, gyrB gene phylogeny clearly showed strain 268506 T should be assigned to the genus Micromonospora but different from any established Micromonospora species. The predominant menaquinones are MK-9(H 8) and MK-9(H 6). The major fatty acids are iso-C 16:0, iso-C 15:0 and anteiso-C 17:0. The characteristic whole-cell sugars are xylose, mannose and arabinose. The cell wall contains meso-DAP and glycine. Phosphatidylinositol, diphosphatidylglycerol and phosphatidylethanolamine are the characteristic polar lipids. The DNA G+C content is 70.3 mol%. Some physiological and biochemical properties combined with low DNA–DNA relatedness indicated that the novel strain could be readily distinguished from the closest phylogenetic relatives. On the basis of these phenotypic and genotypic data, strain 268506 T represents a novel species of the genus Micromonospora, for which the name Micromonospora avicenniae sp. nov. is proposed. The type strain is 268506 T ( = CCTCC AA 2012010 T = DSM 45758 T). 相似文献
6.
A Gram-stain negative, aerobic, oxidase and catalase positive, non-flagellated, pink coloured bacterium with gliding motility, designated as strain UDD1 T was isolated from soil. The bacterium lacked flexirubin-type pigments. Phylogenetic analysis based on its 16S rRNA gene sequence revealed that strain UDD1 T formed a lineage within the family Cytophagaceae of the phylum Bacteroidetes, and forms a distinct clade with type strains of the closely related genus Pontibacter with similarities of 91.36–93.62%. Strain UDD1 T contained MK-7 as the predominant menaquinone and summed feature 4 (iso-C 17:1 I and/or anteiso-C 17:1 B) and iso-C 15:0 as the major fatty acids. The major polar lipids were phosphatidylethanolamine and an unidentified glycolipid. The DNA G+C content of strain UDD1 T was 49 mol%. On the basis of phenotypic, genotypic and phylogenetic analyses, the strain UDD1 T represents a novel species of a new genus in the family Cytophagaceae, for which the name Edaphorhabdus rosea gen. nov., sp. nov., is proposed. The type strain of Edaphorhabdus rosea is UDD1 T (=?KCTC 62117 T =?JCM 32366 T). The Digital Protologue Database Taxon number for strain UDD1 T is GA00058. 相似文献
7.
A novel halophilic actinomycete strain, H23 T, was isolated from a Saharan soil sample collected in Djamâa (Oued Righ region), El-Oued province, South Algeria. Strain H23 T was identified as a member of the genus Actinopolyspora by a polyphasic approach. Phylogenetic analysis showed that strain H23 T had 16S rRNA gene sequence similarities ranging from 97.8 % ( Actinopolyspora xinjiangensis TRM 40136 T) to 94.8 % ( Actinopolyspora mortivallis DSM 44261 T). The strain grew optimally at pH 6.0–7.0, 28–32 °C and in the presence of 15–25 % (w/v) NaCl. The substrate mycelium was well developed and fragmented with age. The aerial mycelium produced long, straight or flexuous spore chains with non-motile, smooth-surfaced and rod-shaped spores. Strain H23 T had MK-10 (H 4) and MK-9 (H 4) as the predominant menaquinones. The whole micro-organism hydrolysates mainly consisted of meso-diaminopimelic acid, galactose and arabinose. The diagnostic phospholipid detected was phosphatidylcholine. The major cellular fatty acids were anteiso-C 17:0 (37.4 %), iso-C 17:0 (14.8 %), iso-C 15:0 (14.2 %), and iso-C 16:0 (13.9 %). The genotypic and phenotypic data show that the strain represents a novel species of the genus Actinopolyspora, for which the name Actinopolyspora righensis sp. nov. is proposed, with the type strain H23 T (=DSM 45501 T = CCUG 63368 T = MTCC 11562 T). 相似文献
8.
A Gram-negative, aerobic, non-flagellated, non-gliding and rod-shaped bacterial strain, designated M-M16 T, was isolated from seashore sand around a seaweed farm on the South Sea, South Korea, and its taxonomic position was investigated by using a polyphasic study. Strain M-M16 T grew optimally at 30 °C, at pH 7.0–8.0 and in the presence of 2 % (w/v) NaCl. Strain M-M16 T exhibited the highest 16S rRNA gene sequence similarity values to the type strains of Gaetbulibacter lutimaris (96.5 %) and Flaviramulus basaltis (95.8 %). Neighbour-joining and maximum-parsimony phylogenetic trees based on 16S rRNA gene sequences revealed that strain M-M16 T clustered with the type strains of Gaetbulibacter species and F. basaltis. Strain M-M16 T contained MK-6 as the predominant menaquinone and iso-C 15:1 G, iso-C 15:0 and iso-C 17:0 3-OH as the major fatty acids. The major polar lipids detected in strain M-M16 T were phosphatidylethanolamine and one unidentified lipid. The DNA G+C content of strain M-M16 T was 37.4 mol%. The phylogenetic and chemotaxonomic data and other phenotypic properties revealed that strain M-M16 T represents a novel genus and species within the family Flavobacteriaceae, for which the name Sabulilitoribacter multivorans gen. nov., sp. nov. is proposed. The type strain of S. multivorans is M-M16 T (= KCTC 32326 T = CCUG 63831 T). 相似文献
9.
Three halophilic mycelium-forming actinobacteria, strains H195 T, H150 and H151, were isolated from a Saharan soil sample collected from Béni-isguen in the Mzab region (Ghardaïa, South of Algeria) and subjected to a polyphasic taxonomic characterisation. These strains were observed to show an aerial mycelium differentiated into coccoid spore chains and fragmented substrate mycelium. Comparative analysis of the 16S rRNA gene sequences revealed that the highest sequence similarities were to Saccharopolyspora qijiaojingensis YIM 91168 T (92.02 % to H195 T). Phylogenetic analyses showed that the strains H195 T, H150 and H151 represent a distinct phylogenetic lineage. The cell-wall hydrolysate was found to contain meso-diaminopimelic acid, and the diagnostic whole-cell sugars were identified as arabinose and galactose. The major cellular fatty acids were identified as iso-C 15:0, iso-C 16:0, iso-C 17:0 and anteiso-C 17:0. The diagnostic phospholipid detected was phosphatidylcholine and MK-9 (H 4) was found to be the predominant menaquinone. The genomic DNA G+C content of strain H195 T was 68.2 mol%. On the basis of its phenotypic features and phylogenetic position, we propose that strain H195 T represents a novel genus and species, Mzabimyces algeriensis gen. nov., sp. nov., within a new family, Mzabimycetaceae fam. nov. The type strain of M. algeriensis is strain H195 T (=DSM 46680 T = MTCC 12101 T). 相似文献
10.
A novel bacterium, designated JP1 T, was isolated from soil of Hallasan Mountain in Jeju Island. The isolate was a Gramnegative, aerobic, motile and rod-shaped (0.2–0.4 × 1.2–2.0 μm) bacterium. Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain JP1 T was closely related to Dyella koreensis with 97.6% similarity. Growth of strain JP1 T occurred at 10–37°C, pH 5–7 and 0–1% (w/v) NaCl. The genomic DNA G+C content of strain JP1 T was 62.1 mol%. The major fatty acids were iso-C 16:0, iso-C 17:1 ω9c, and iso-C 15:0. The predominant quinone was ubiquinone-8. The major polar lipids of strain JP1 T were phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, unidentified aminolipids and unidentified aminophospholipids. The DNA-DNA relatedness values between strain JP1 T and previously reported Dyella species were <10%. Based on phenotypic, genotypic, and phylogenetic distinctness, strain JP1 T represents a novel species in the genus Dyella, for which the name Dyella jejuensis sp. nov. is proposed. The type strain is JP1 T (=KACC 17701 T =JCM 19615 T). 相似文献
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