A Cytotoxic Neolignan from Schisandra propinqua （Wall.） Baill.

In the course of our study of bioactive natural products from Schisandra plants, we isolated a neolignan from an EtOAc extract of the stems of Schisandra propinqua （Wall.） Baill. The structure of the new com- pound was determined to be 4, 4-di （4-hydroxy-3-methoxyphenly）-2, 3-dimethylbutanol （compound 1） on the basis of 1H- and 13C-NMR spectra and 2D NMR methods. Eight known compounds, compounds 2-9, were also isolated and identified, of which compounds 3, 4, 6 and 9 were isolated for the first time from this plant. In addition, compounds 1-4 were evaluated for cytotoxicity by an 3-（4,5-dimethyl-2 thiazoyl）-2,5-diphenyl-2H- tetrazolium bromide （MTT） assay. Compound 1 showed significant potential cytotoxic ability in the bioassay.     

Chemical Constituents of the Roots of Anemone altaica Fisch. ex C. A. Mey.

The roots of Anemone altaica Fisch. ex C. A. Mey. have been used in the treatment of epilepsia, neurasthenia, and arthritis in Chinese folk medicine for a long time. In order to find new and bioactive compounds, the chemical constituents of the roots of A. altaica were investigated and nine compounds were isolated from the EtOH extract of this plant. On the basis of spectroscopic methods, the structures of these compounds were elucidated as 4-（9H-β-carbolin- 1-yl）-4-oxo-butyric acid （1）, carboxymethyl isoferulate （2）, isoferulic acid （3）, cirsiumaldehyde （4）, 5-hydroxy-4-oxo-pentanoic acid （5）, triacontane （6）, palmic acid （7）, β-sitosterol （8）, and daucosterol （9）. Among them, 1 and 2 were new compounds, and 3 and 4 were obtained from this genus for the first time.     

A New Isoflavonoid from Belamcanda chinensis （L.） DC.

A new isoflavonoid, 5, 6, 7, 3＇-terahydroxy-8, 4＇, 5＇-trimethoxyisoflavone （1）, along with 10 known isoflavonoids, namely 5, 6, 7, 4＇-tetrahydroxy-8-methoxyisoflavone （2）, irilone （3）, genistein （4）, tectorigenin （5）, irigenin （6）, irisflorentin （7）, dichotomitin （8）, dimethyltectorigenin （9）, iridin （10）, and tectoridin （11）, was isolated from the alcohol extract of the rhizomes of Belamcanda chinensis （L.） DC. The structures of these compounds were elucidated on the basis of results of spectroscopic analysis.     

A New Pregnane from Munronia delavayi Franc （Meliaceae）

To search for pharmacologically active constituents of folk medicine, a new pregnane, 2α,3α,15β-trihydroxy-20（S）-tigloyl-pregnane （compound 1）, and nine known compounds, geranylgeraniol （compound 2）, β-daucosterol （compound 3）, 6-hydroxystigmast-4oen-3-one （compound 4）, sitoindoside Ⅰ （compound 5）, sitoindoside Ⅱ （compound 6）, β-sitosterol （compound 7）, kaempferol （compound 8）, quercetin （compound 9）, and rutin （compound 10）, were isolated from the ethanol extract of whole plants of Munronia delavayi Franch using chromatographic methods. The structures of compounds 1-10 were elucidated on the basis of spectral data.     

Two New Coumarins from Fraxinus chinensis Rexb.

The ethanol extract of the stem bark of Fraxinus chinensis Roxb. showed good inhibitory bioactivity against VEGF receptor-1 kinase （IC50：13.8 μg/mL）. In order to find new and bioactive compounds, the chemical constituents of the stem bark of F. chinensis were investigated and two new coumarins, namely 6＇-O-sinapinoyl esculin （compound 1） and 6＇-O-vanillyl esculin （compound 2）, together with eleven known compounds （compounds 3-13） were isolated. The structures of the two new compounds were identified by their physicochemical properties and spectral analysis, particularly one- and two-dimensional nuclear magnetic resonance spectral methods. The known compound, oleuropein （compound 11）, exhibited moderate activity （IC50：（8.7 ± 1.3） μmol/L） to inhibit VEGF receptor- 1 kinase.     

Chemical Constituents of Daphne giraldii Nitsche

In a search for structurally interesting substances from traditional Chinese medicines, eight compounds were isolated from an ethanolic extract of the stem bark of Daphne giraldii Nitsche. On the basis of one- and two-dimensional nuclear magnetic resonance and mass spectrometry data, and chemical methods, their structures were determined to be 2H-1-benzopyran-2-one-8-hydroxy-7-O-β-D-glucopyranosyl-5-（2-oxo- 2H-1-benzopyran-7-hydroxy-8-yloxy） （compound 1）, 1-pentanone, 1-（4-hydroxyphenyl）-5-phenyl （compound 2）, octadecyl caffeate （compound 3）, （＋）syringaresinol-4,4＇-diglycoside （compound 4）, daphnetin-8-O-β-D- glucopyranoside （compound 5）, p-hydroxybenzoic acid （compound 6）, 7,7＇-dihydroxy-[6, 8＇-bi-2H-benzopyran]- 2, 2＇-dione （compound 7）, and daphnorin （compound 8）, respectively. Of the compounds isolated, compounds 1 and 2, which we named daphnolin and daphnolon, respectively, were new, and the others were obtained from this plant for the first time.     

Water-Soluble Constituents of Cudrania tricuspidata （Carr.） Bur.

In order to find new structural and biologically active compounds, the constituents of the bark of Cudrania tricuspidata （Carr.） Bur. were investigated and a new 6-p-hydroxybenzyltaxifolin glucoside, named tricusposide （compound 1）, together with 16 known compounds, was isolated by solvent partition, macroporous adsorption resin AB-8, silica gel, Sephadex LH-20 chromatography. Using spectroscopic methods, the structures of the compounds were elucidated as 6-p-hydroxybenzyl taxifolin-7-O-β-D-glucoside （compound 1）, dihydroquerctin-7-O-β-D-glucoside （compound 2）, dihydrokaempferol-3-O-β-D-glucoside （compound 3）, dihydroquercetin （compound 4）, peonoside （compound 5）, sphaerobioside （compound 6）, quercimeritrin （compound 7）, genistein （compound 8）, aromadendrin （compound 9）, kaempferol （compound 10）, genistin （compound 11）, 3,4-dihydroxystyryl alcohol （compound 12）, sucrose （compound 13）, 1,3,5,6-tetrahydroxyxanthone （compound 14）, gericudranin E （compound 15）, gericudranin C （compound 16）, and orobol （compound 17）. Compounds 2-6, 8, 9, 12-14, and 17 were isolated from this genus for the first time.     

Chemical Constituents of the Roots of Vernonia cumingiana Benth.

To search for new and bioactive constituents from traditional Chinese medicines, a new steroidal saponin, named vernonioside G （1）, was isolated from the roots of Vernonia cumingiana Benth. （Compositae）. The structure of vernonioside G was elucidated using spectral methods, particularly two-dimensional nuclear magnetic resonance analysis. Together with the new compound, eight known compounds were also isolated and identified from the roots of V. cumingiana, among which, VE-1 （2） and 24-methylenelanost-9（11）- en-3β-ol acetate （3） were assigned NMR data for the first time and compound 3 was obtained as a natural product from a plant for the first time.     

Chemical Constituents of Siegesbeckia orientalis L.

The aerial parts of Siegesbeckia have been used as the traditional Chinese medicine in the treatment of rheumatic arthritis, hypertension, malaria, neurasthenia, and snakebite. In order to find new and bioactive compounds, the chemical constituents of the aerial parts of S. orientalis L. were investigated and two new compounds, namely β-D-glucopyranosyl-ent-2-oxo-15,16-dihydroxy-pimar-8（14）-en-19-oic-late （compound 1 ） and [1 （10） E,4Z]-8β-angeloyloxy-9α-methoxy-6α, 15-dihydroxy-14-oxogermacra-1 （10）,4,11 （13）-trien-12-oic acid 12,6-1actone （compound 2）, as well as five known ent-pimarane diterpenes （compounds 3-7） were isolated. The structures of the two new compounds were identified by their physicochemical properties and spectral analysis, particularly oneand two-dimensional nuclear magnetic resonance spectral methods.     

A Novel Flavonoid Glucoside from Anoectochilus roxburghii （Wall,） Lindl.

Eight compounds were isolated from the ethyl acetate- and n-butanol-soluble fractions of the ethanollc extract of the whole plant of Anoectochllus roxburghll（Wali.） Lindi. （Orchidaceee）. On the basis of spectroscopic methods, the structures of these compounds were elucidated as quercetin-7-O-β-D-[6＂-O-（trans-feruloyi）]- giucopyranosids （compound 1）, 8-C-p-HydroxybenzyiquerceUn （compound 2）, isorhamnetin-7-O-β-D- giucopyranoside （compound 3）, isorhamnetin-3-O-β-D-giucopyranoside （compound 4）, kaempferoi-3-O-β-D- giucopyranoside （compound 5）, kaempferoi-7-O-β-D-giucopyranoside （compound 6）, 5-hydroxy-3＇,4＇,7- trimethoxyfiavonoi-3-O-β-D-rutinoside （compound 7）, and isorhamnetin-3-O-β-D-rutinoside （compound 8）. Of the compounds isoisted, compound 1 was a new flavonoid giucoside and exhibited strong scavenging activity against the 1,1-diphenyi-2-picryihydrazyi free radical, whereas the ethanolic extract showed weak activity. Compounds 2-8 were obtained from this family for the first time.     

团花树皮的化学成分研究

采用硅胶、MCI和Sephadex LH-20层析方法对团花树皮的化学成分进行分离纯化,运用现代波谱技术鉴定了10个化合物:4-carboxy-3-hydroxy-5-methylphenyl 3-methoxy-4-hydroxy-5-methylbenzoate(1),谷甾醇-3-O-(6’-O-棕榈酰基)-β-D-葡萄糖苷(2),喹诺酸-3-O-α-L-鼠李糖苷(3),clethric acid(4),常春藤苷元(5),钩藤苷元C(6),morolic acid(7),咖啡酸甲酯(8),卡丹宾(9)和3α-二氢卡丹宾(10)。其中化合物1为一个新的酚性成分,化合物2～8首次从该属植物中分离得到。     

A New Isofiavonoid from Belamcanda chinensis (L.) DC.

A new isoflavonoid, 5, 6, 7, 3'-terahydroxy-8, 4', 5'-trimethoxyisoflavone (1), along with 10 known isoflavonoids, namely 5, 6, 7, 4'-tetrahydroxy-8-methoxyisoflavone (2), irilone (3), genistein (4), tectorigenin (5), irigenin (6), irisflorentin (7), dichotomitin (8), dimethyltectorigenin (9), iridin (10), and tectoridin (11), was isolated from the alcohol extract of the rhizomes of Belamcanda chinensis (L.) DC. The structures of these compounds were elucidated on the basis of results of spectroscopic analysis.     

中国红豆杉枝叶中的紫杉烷二萜

从中国红豆杉(Taxus chinensis)枝叶的乙醇提取物中分离得到8个紫杉烷二萜,通过波谱分析分别确定为：14p羟基巴卡亭Ⅵ(1),巴卡亭Ⅵ(2),巴卡亭Ⅳ(3),1β-去羟基巴卡亭Ⅳ(4),云南红豆杉酯甲(5),2α-去乙酰-2α-苯甲酰基-13α-乙酰基云南紫杉亭(6),5α-羟基-2α,7βp,9α,10β,13α-五乙酰氧基紫杉-4(20),11-二烯(7)和Taxaein(8)。其中化合物1为新化合物,并报道八个化合物在丙酮中测得的核磁共振信号,化合物8的数据属首次报道。     

中国红豆杉中一个新的紫杉烷二萜

从中国红豆杉(Taxus chinensis)枝叶中分离得到4个紫杉烷二萜,通过波谱分析分别确定为：1β-羟基-2α,7β-二去乙酰基巴卡亭I(1),1β-羟基巴卡亭I(2),2α,5α,7β,9α,10β,13α-六乙酰氧基紫杉-4(20),11-二烯(3)和2-去乙酰氧基-5-去肉桂酰taxinine J(4)其中化合物1为新化合物。     

中华青荚叶的一个新果糖酯

从山茱萸科中华青荚叶( Helwingia chinensis )的乙醇提取物中分离得到一个新果糖酯和十个已知化合物.通过现代波谱技术分别鉴定为:2- O -(E)-咖啡酰-3- O -(3, 5-二甲氧基香豆酰)-α-D-呋喃果糖甙(1),2- O -β-D-呋喃果糖基α-D-异吡喃糖酯(2),甘草甜素(3),4′-羟基-7- O -葡萄糖-2, 3-二羟黄酮甙(4),黄豆甙(5),5-葡萄糖芹菜甙(6),7- O -葡萄糖芹菜甙(7),4- O -葡萄糖香豆酸(8), 葡萄糖咖啡酸(9), 3β-赤杨醇(10), 薯蓣皂甙3- O -{α-L-鼠李糖吡喃糖基(1→2)-[α-L-阿拉伯呋喃糖基(1→3)]-β-D-葡萄糖吡喃糖} (11).     

竹叶榕根的化学成分研究

从竹叶榕(Ficus stenophyllaHemsl.)根乙醇提取物中分离得到10个化合物,经理化常数和波谱数据分析,分别鉴定为3,4-二氢补骨酯素(3,4-dihydropsoralen,1)、7-羟基香豆素(7-hydroxycoumarin,2)、香柠檬内酯(bergapten,3)、补骨酯素(psoralen,4)、( )-儿茶素(catechin,5)、芹菜素(apigenin,6)、蔗糖(sucrose,7)、香草酸(vanillic acid,8)、胡萝卜苷(daucosterol,9)和豆甾醇(stigmasterol,10)。化合物1~10均为首次从该植物中分离得到,其中化合物1为新天然产物。     

华麻花头根中的蜕皮甾酮类成分

从华麻花头(Serratula chinensis S．Moore)根中分得7种蜕皮甾酮类化合物,经光谱分析和化学方法,分别鉴定为：20-羟基蜕皮松(1),podecdysone C(2),3-氧-乙酰基-20-羟基蜕皮松(3),20-羟基蜕皮松-20,22．-缩丁醛(4),shidasterone(5),atrotosterone C(6)和carthamosterone(7),其中20-羟基蜕皮松-20,22．缩丁醛为一新的化合物。     

Anti-adipogenic chromone glycosides from Cnidium monnieri fruits in 3T3-L1 cells

Seven new chromone glycosides, monnierisides A (3), B (10), C (11), D (12), E (13), F (15) and G (16) were isolated from Cnidium. monnieri, together with ten known chromone derivatives, undulatoside A (1), cnidimol C (2), saikochromoside A (4), cnidimoside A (5), cnidimoside B (6), 2-methyl-5-hydroxy-6-(2-butenyl-3-hydroxymethyl)-7-(β-d-glucopyranosyloxy)-4H-1-benzopyran-4-one (7), cnidimol D (8), hydroxycnidimoside A (9), umtatin (14) and 6'-hydroxylangelicain (17). The structures of isolated compounds were determined on the basis of spectroscopic analysis including 1D, 2D NMR and HR-MS. Among the compounds isolated, compounds 5, 6, 9 and 10 significantly inhibited adipocyte differentiation as measured by fat accumulation in 3T3-L1 cells using Oil Red O staining.     

细花线纹香茶菜的化学成分研究

用色谱技术对细花线纹香茶菜(Isodon lophanthoides var.graciliflora)全草水提物进行分离纯化,获得了11种单体化合物。通过光谱分析及与文献数据对照,分别鉴定为牡荆素(vitexin,1)、藿香黄酮醇(pachypodol,2)、猫眼草黄素(chrysoplenetin,3)、9-羟基-5,7-豆甾二烯-4-酮(9-hydroxy-5,7-megastigmadien-4-one,4)、3,9-二羟基-5,7-豆甾二烯-4-酮(3,9-dihydroxy-5,7-megastigmadien-4-one,5)、黑麦草内酯(loliolide,6)、4-乙酰-5-甲基二氢呋喃-2-酮(4-acetyl-5-methyldihydrofuran-2-one,7)、5-羟甲基糠醛(5-hydroxymethylfuraldehyde,8)、5-甲氧甲基-1H-吡咯-2-甲醛(5-methoxymethyl-1H-pyrrole-2-carbaldehyde,9)、3-吲哚甲醛(indole-3-aldehyde,10)和丁二酸单丁酯(succini cacid monobutyl ester,11)。这些化合物均为首次从线纹香茶菜中分离得到。