CO_2激光治疗小儿面颈部色素痣14例疗效观察

ＣＯ２激光治疗小儿面颈部色素痣１４例疗效观察李小标（武汉市儿童医院皮肤科，４３００１６）关键词色素痣ＣＯ２激光ＥｒａｌｕａｔｉｏｎｏｎＥｆｅｃｔｏｆ１４ｃａｓｅｓｃｈｉｌｄｒｅｎ′ｓＮｅｒｕｓＰｉｇａｍｅｎｔｏｓｕｓｏｎＦａｅｅａｎｄＮｅｃｋｗｉｔｈ...     

不同波长激光辐照花生种子的生物学效应

本实验考察了Ｋ＋ｒ、Ａｒ＋、Ｎｄ：ＹＡＧ、ＨｅＮｅ和ＬＤ等不同波长的激光辐照对花生种子产生的生物学效应。结果显示,适当剂量不同波长的激光辐照都能促进花生种子生长。在辐照剂量为０．１２８ｗ／ｃｍ２×１８０ｓ的条件下,较短波长激光对花生幼苗的促进作用比长波长激光显著;在辐照剂量为１．２８ｗ／ｃｍ２×１８ｓ的条件下,短波长激光对花生种子的萌发及胚的生长有抑制作用,而长波长激光有促进效应。在相同辐照剂量条件下,不同功率密度与时间的组合其辐照效果不同。１．２８ｗ／ｃｍ２功率密度的Ｎｄ：ＹＡＧ（５３２ｎｍ）激光脉冲输出辐照对花生种子的生长产生显著的抑制作用。实验结果提示,要得到相同的辐照效果,长波长激光与短波长激光相比,必须提高辐照功率密度或加大辐照输出剂量。     

CO2激光辐照对酿酒酵母菌的诱变作用

应用红外ＣＯ２激光对甘蔗糖蜜工业性生产用酿酒酵母菌Ｓａｃｃｈａｒｏｍｙｃｅｓ ｃｅｒｅｖｉｓｉａｅ ＡＳ２．１１８９进行辐照处理,经酵母菌糖蜜酒精发酵试验,发现红外ＣＯ２激光对酿酒酵母菌具有诱变作用,并初步筛选到决乙醇含量有较大变化的辐照变异菌株;同时,通过对这些辐照变异菌株的乙醇脱氢酶同工酶的比较分析,进一步证实了红外ＣＯ２激光对酿酒酵母酶的诱变作用。从而为工业上利用红外ＣＯ２激光对酿酒酵母菌进     

沈阳市区大气SO2污染程度的叶绿素含量监测分析

沈阳市区大气ＳＯ２污染程度的叶绿素含量监测分析周兴文（沈阳大学师范学院生物系,沈阳１１００１５）ＴｈｅｃｈｌｏｒｏｐｈｙｌｃｏｎｔｅｎｔｍｏｎｉｔｏｒｉｎｇａｎａｌｙｓｉｓｏｆｔｒｅｌｅａｖｅｓｆｏｒＳＯ２ｐｏｌｕｔｉｏｎｉｎＳｈｅｎｙａｎｇＣｉｔｙ...     

激光照射,免疫抑制剂及二者配合应用对大鼠全胰十二指肠移植的影…

以Ｗｉｓｔａｒ糖尿病模型大鼠作为受体，进行同性间全胰十二指肠移植。用激光照射、免疫抑制剂及二者配合应用进行抗移植排斥反应试验，于术后７天及出现排斥反应相时，进行病理解剖与病理组织学观察，结果表明，日剂量为３９。７２４５Ｊ／ｃｍ＾２的激光照射，可推迟排斥反应的发生时间、降低排斥反应的发生程度；激光照射与８－５－３ｍｇ／ｋｇ／ｄａｙ的硫唑嘌呤（Ａｚａ）配合，上述作用显著，且与环孢霉素Ａ（ＣｓＡ）的效果     

若干蝙蝠葛碱衍生物对人红细胞膜Ca^2＋—Mg^2＋——ATPase活力的影响

本文测定了数种蝙蝠葛碱衍生物对钙调素激活的人红细胞膜Ｃａ＾２＋－Ｍｇ＾２＋－ＡＴＰａｓｅ和的影响,结果表明,这些化合物对该酶都有不同程度的抑制作用,其机制表现为性抑制,过量的ＣａＭ能完全逆转这些化合物所引起的抑制。当Ｃａ＾２＋－Ｍｇ＾２＋－ＡＴＰａｓｅ被胰蛋白酶限制性酶解完全活化后,其活力不再受ＣａＭ激活,但仍被这些化合物所抑制。     

芦苇（Phragmites communis）光合作用与蒸腾作用的日进程

测定了野生芦苇（Ｐｈｒａｇｍｉｔｅｓｃｏｍｍｕｎｉｓ）光合作用、蒸腾作用及相关指标的日变化。叶片的净光合作用率在１０∶００～１５∶００间保持一个高的稳定阶段,平均为７３～９９μｍｏｌＣＯ２·ｍ－２·ｓ－１,光饱和状态下的最大值为１２７μｍｏｌＣＯ２·ｍ－２·ｓ－１,处于挺水植物的低限。蒸腾作用强度的峰值达４８ｍｍｏｌＨ２Ｏ·ｍ－２·ｓ－１。光合作用和蒸腾作用的日进程均未发生“午休”现象;相反,二者都是在正午前后强度最大。光合光量子流密度与净光合作用率、蒸腾强度、叶面传导率均存在不同程度的正相关。芦苇的光合作用强度和水分利用效率相对较低,但年生产量却较高,这是因为芦苇具有密实的叶子、有利的叶片配置结构和高的蒸腾作用强度。     

DOPE、DOPC对重组去脂肌质网Ca~(2+)-ATPase活力和构象的影响

经磷脂酶Ａ２ 去脂的肌质网Ｃａ２ ＋ － ＡＴＰａｓｅ 重组于不同比例的二油酰磷脂酰胆碱（Ｄｉｏｌｅｏｙｌｐｈｏｐｈａｔｉｄｙｌｃｈｏｌｉｎｅ,ＤＯＰＣ） 和二油酰磷脂酰乙醇胺（Ｄｉｏｌｅｏｙｌｐｈｏｐｈａｔｉｄｙｌｅｔｈａｎｏｌａｍｉｎｅ,ＤＯＰＥ） 形成脂酶体,研究了不同磷脂环境中Ｃａ２ ＋ － ＡＴＰａｓｅ 的ＡＴＰ 水解和Ｃａ２ ＋ 转运活力。结果表明,ＤＯＰＣ 和ＤＯＰＥ 分别有利于ＡＴＰ 水解和Ｃａ２ ＋ 的转运,ＤＯＰＥ 可以增强Ｃａ２ ＋ － ＡＴＰａｓｅ 的ＡＴＰ水解和Ｃａ２ ＋ 转运之间的偶联效率。利用内源荧光、荧光淬灭及Ｆｏｒｓｔｅｒ 能量转移原理测定Ｃａ２ ＋ －ＡＴＰａｓｅ 相应的构象变化, 发现随着ＤＯＰＥ／ ＤＯＰＣ 比例的改变使Ｃａ２ ＋ － ＡＴＰａｓｅ 构象发生相应的变化。     

青藏高原毛茛科3种特有植物核型和进化研究

对青藏高原高山冰缘地区毛茛科３种特有植物的核型进行了分析。它们的核型公式（Ｋ）、染色体相对长度组成（Ｃ．Ｒ．Ｌ．）和核型不对称系数（Ａｓ．Ｋ％）分别为：青藏金莲花Ｔｒｏｌｉｕｓｐｕｍｉｌｕｓｖａｒ．ｔａｎｇｕｔｉｃｕｓ：Ｋ（２ｎ）＝６ｍ＋８ｓｍ（２ＳＡＴ）＋２ｓｔ,Ｃ．Ｒ．Ｌ．＝４Ｌ＋４Ｍ２＋４Ｍ１＋４Ｓ,Ａｓ．Ｋ％＝６３．５７,核型属２Ｂ型;甘青乌头Ａｃｏｎｉｔｕｍｔａｎｇｕｔｉｃｕｍ为Ｋ（２ｎ）＝６ｍ＋１０ｓｍ,Ｃ．Ｒ．Ｌ．＝４Ｌ＋８Ｍ１＋４Ｓ,Ａｓ．Ｋ％＝６２．５４,２Ｂ型;单花翠雀花Ｄｅｌｐｈｉｎｉｕｍｃａｎｄｅｌａｂｒｕｍｖａｒ．ｍｏｎａｎｔｈｕｍ为Ｋ（２ｎ）＝６ｍ＋８ｓｍ＋２ｓｔ,Ｃ．Ｒ．Ｌ．＝４Ｌ＋４Ｍ２＋４Ｍ１＋６Ｓ,Ａｓ．Ｋ％＝６４．３４,属３Ｂ型。经同相关近缘种核型资料比较,青藏金莲花核型不对称性和进化程度比金莲花Ｔ．ｃｈｉｎｅｎｓｉｓ低;甘青乌头的核型不对称性和进化程度在其近缘类群（乌头组Ｓｅｃｔ．Ａｃｏｎｉｔｕｍ）已报道的种之内最低;单花翠雀花核型不对称性和进化水平比翠雀组（Ｓｅｃｔ．Ｄｅｌｐｈｉｎａｓｔｒｕｍ）已报道的展毛翠雀花Ｄ．ｋａｍａｏｅｎｓｅｖａｒ．ｇｌａｂｒｅｓｃｅｎｓ、     

鹅观草属五个类群的核型与进化

报道了鹅观草属５个类群的核型,即长芒鹅观草,核型２ｎ＝４ｘ＝２８＝２２ｍ＋６ｓｍ（２ＳＡＴ）;短颖鹅观草,核型２ｎ＝４ｘ＝２８＝２０ｍ（２ＳＡＴ）＋８ｓｍ（２ＳＡＴ）;短柄鹅观草,核型２ｎ＝４ｘ＝２８＝２２ｍ（２ＳＡＴ）＋６ｓｍ;纤毛鹅观草,核型２ｎ＝４ｘ＝２８＝２０ｍ＋８ｓｍ（４ＳＡＴ）;毛盘鹅观草,核型２ｎ＝４ｘ＝２８＝１８ｍ＋６ｓｍ（４ＳＡＴ）＋４ｓｔ。同时,通过核型重要性状的递变分析,揭示了鹅观草属５个类群的相对进化程度以及宏观分类中４个组的系统发育关系,表明鹅观草属的半颖组在系统发育中可能既派生了颖体短小的小颖组,又派生了颖体长大的大颖组和长颖组。     

The effects of temperature and swimming speed on instantaneous fuel use and nitrogenous waste excretion of the Nile tilapia.

The effects of acclimation temperature (30 degrees, 20 degrees, and 15 degrees C) and swimming speed on the aerobic fuel use of the Nile tilapia (Oreochromis niloticus; 8-10 g, 8-9-cm fork length) were investigated using a respirometric approach. As acclimation temperature was decreased from 30 degrees C to 15 degrees C, resting oxygen consumption (Mo2) and carbon dioxide excretion (Mco2) decreased approximately twofold, while nitrogenous waste excretion (ammonia-N plus urea-N) decreased approximately fourfold. Instantaneous aerobic fuel usage was calculated from respiratory gas exchange. At 30 degrees C, resting Mo2 was fueled by 42% lipids, 27% carbohydrates, and 31% protein. At 15 degrees C, lipid use decreased to 21%, carbohydrate use increased greatly to 63%, and protein use decreased to 16%. These patterns at 30 degrees C and 15 degrees C in tilapia paralleled fuel use previously reported in rainbow trout acclimated to 15 degrees C and 5 degrees C, respectively. Temperature also had a pronounced effect on critical swimming speed (UCrit). Tilapia acclimated to 30 degrees C had a UCrit of 5.63+/-0. 06 body lengths/s (BL/s), while, at 20 degrees C, UCrit was significantly lower at 4.21+/-0.14 BL/s. Tilapia acclimated to 15 degrees C were unable or unwilling to swim. As tilapia swam at greater speeds, Mo2 increased exponentially; Mo2min and Mo2max were 5.8+/-0.6 and 21.2+/-1.5 micromol O2/g/h, respectively. Nitrogenous waste excretion increased to a lesser extent with swimming speed. At 30 degrees C, instantaneous protein use while swimming at 15 cm/s ( approximately 1.7 BL/s) was 23%, and at UCrit (5.6 BL/s), protein use dropped slightly to 17%. During a 48-h swim at 25 cm/s (2.7 BL/s, approximately 50% UCrit), Mo2 and urea excretion remained unchanged, while ammonia excretion more than doubled by 24 h and remained elevated 24 h later. These results revealed a shift to greater reliance on protein as an aerobic fuel during prolonged swimming.     

Effect of temperature and host-parasite ratio on sex differentiation of Romanomermis iyengari (Welch), a mermithid parasite of mosquitoes

The effect of temperature and host-parasite ratio on the percentage infection and sex differentiation of R. iyengari was studied. Significant differences were observed in the percentage infection due to different host-parasite ratios and temperatures. At 25 degrees and 30 degrees C, the host parasite ratio of 1:3 resulted in 86-92% infection of Culex quinquefasciatus larvae. At 20 degrees and 35 degrees C, a higher host-parasite ratio was required to get this level of infection. More number of post-parasites per mosquito larva emerged at 20 degrees (1.5-5.8) and 25 degrees C (1.9-6.3) than at 30 degrees (1.5-3.9) and 35 degrees C (1.6-3.6). More than 50% of the post-parasites were females at 20 degrees and 25 degrees, 30 degrees and 35 degrees C at 1:1-1:10, 1:1-1:4 and 1:1-1:3 host-parasite ratios, respectively.     

Leaf respiration of snow gum in the light and dark. Interactions between temperature and irradiance

We investigated the effect of temperature and irradiance on leaf respiration (R, non-photorespiratory mitochondrial CO(2) release) of snow gum (Eucalyptus pauciflora Sieb. ex Spreng). Seedlings were hydroponically grown under constant 20 degrees C, controlled-environment conditions. Measurements of R (using the Laisk method) and photosynthesis (at 37 Pa CO(2)) were made at several irradiances (0-2,000 micromol photons m(-2) s(-1)) and temperatures (6 degrees C-30 degrees C). At 15 degrees C to 30 degrees C, substantial inhibition of R occurred at 12 micromol photons m(-2) s(-1), with maximum inhibition occurring at 100 to 200 micromol photons m(-2) s(-1). Higher irradiance had little additional effect on R at these moderate temperatures. The irradiance necessary to maximally inhibit R at 6 degrees C to 10 degrees C was lower than that at 15 degrees C to 30 degrees C. Moreover, although R was inhibited by low irradiance at 6 degrees C to 10 degrees C, it recovered with progressive increases in irradiance. The temperature sensitivity of R was greater in darkness than under bright light. At 30 degrees C and high irradiance, light-inhibited rates of R represented 2% of gross CO(2) uptake (v(c)), whereas photorespiratory CO(2) release was approximately 20% of v(c). If light had not inhibited leaf respiration at 30 degrees C and high irradiance, R would have represented 11% of v(c). Variations in light inhibition of R can therefore have a substantial impact on the proportion of photosynthesis that is respired. We conclude that the rate of R in the light is highly variable, being dependent on irradiance and temperature.     

Effect of temperature on the activity and synthesis of glucose-catabolizing enzymes in Pseudomonas fluorescens.

The activity of the enzymes of the oxidative non-phosphorylated pathway, glucose and gluconate dehydrogenases, were not significantly affected by changes in the assay temperature. Both enzymes demonstrated only a threefold difference in activity when compared at assay temperatures of 30 degrees C and 5 degrees C. In contrast, the enzymes involved in the direct phosphorylation and catabolism of glucose or its oxidation products, gluconate and 2-ketogluconate, exhibited a more pronounced response to decreasing assay temperatures. At least one enzyme in each pathway, involved in the direct phosphorylation and catabolism of glucose or 2-ketogluconate (2KG), demonstrated an eightfold decrease in activity with a decrease in assay temperature from 30 degrees C to 5 degrees C. A similar decrease in assay temperature resulted in a fivefold decrease in activity of the enzymes involved in the direct phosphorylation and catabolism of gluconate. The observed differential effect of temperature on the activity of the enzymes of glucose catabolism and on the accumulation of direct oxidation products during growth with glucose in P. fluorescens E-20 is discussed. Growth with glucose at 5 or 20 degrees C resulted in high induced levels of all glucose-catabolizing enzymes examined when compared with the levels of these same enzymes in pyruvate-grown cells. However, only low levels of glucose dehydrogenase were detected during growth at 30 degrees C with glucose, gluconate, or 2-KG. Similarly, only low levels of gluconate dehydrogenase were detected during growth with glucose at 30 degrees C, although a weak induction was observed during growth with gluconate or 2-KG at 30 degrees C. The levels of 2-KG kinase plus KPG reductase during growth at 30 degrees C were undetectable with glucose, weakly induced with gluconate, and fully induced with 2-KG. High induced levels of glucose dehydrogenase, gluconate dehydrogenase, and 2-KG kinase plus KPG reductase were present during growth at 20 degrees C with glucose or 2-KG. The low levels of glucose and gluconate dehydrogenases present at a growth temperature of 30 degrees C was not due to heat lability of the enzymes at this temperature. The low amounts of these two enzymes during growth with glucose at 30 degrees C probably prevented sufficient inducer(s) formation from glucose to allow induction of enzymes of 2-KG catabolism. The results demonstrated that temperature may regulate the pathways of glucose dissimilation by regulating, either directly or indirectly, the activity and synthesis of the enzymes involved in these pathways.     

Effect of quadriceps femoris muscle length on neural activation during isometric and concentric contractions.

The effect of muscle length on neural drive (here termed "neural activation") was investigated from electromyographic activities and activation levels (twitch interpolation). The neural activation was measured in nine men during isometric and concentric (30 and 120 degrees /s) knee extensions for three muscle lengths (35, 55, and 75 degrees knee flexion, i.e., shortened, intermediate, and lengthened muscles, respectively). Long (76 degrees ), medium (56 degrees ), and short (36 degrees ) ranges of motion were used to investigate the effect of the duration of concentric contraction. Neural activation was found to depend on muscle length. Reducing the duration of contraction had no effect. Neural activation was higher with short muscle length during isometric contractions and was weaker for shortened than for intermediate and lengthened muscles performing 120 degrees /s concentric contractions. Muscle length had no effect on 30 degrees /s concentric neural activation. Peripheral mechanisms and discharge properties of the motoneurons could partly explain the observed differences in the muscle length effect. We thus conclude that muscle length has a predominant effect on neural activation that would modulate the angular velocity dependency.     

Differential effects of temperature on cAMP-induced excitation, adaptation, and deadaptation of adenylate and guanylate cyclase in Dictyostelium discoideum

Extracellular cAMP induces excitation of adenylate and guanylate cyclase in Dictyostelium discoideum. Continuous stimulation with cAMP leads to adaptation, while cells deadapt upon removal of the cAMP stimulus. Excitation of guanylate cyclase by cAMP has a lag time of approximately 1 s; excitation of adenylate cyclase is much slower with a lag time of 30 s. Excitation of both enzyme activities is less than twofold slower at 0 degrees C than at 20 degrees C. Adaptation of guanylate cyclase is very fast (t1/2 = 2.4 s at 20 degrees C), and virtually absent at 0 degrees C. Adaptation of adenylate cyclase is much slower (t1/2 = 110 s at 20 degrees C) but not very temperature sensitive (t1/2 = 290 s at 0 degrees C). At 20 degrees C, deadaptation of adenylate cyclase is about twofold slower than deadaptation of guanylate cyclase (t1/2 = 190 and 95 s, respectively). Deadaptation of adenylate cyclase is absent at 0 degrees C, while that of guanylate cyclase proceeds slowly (t1/2 = 975 s). The results show that excitation, adaptation, and deadaptation of guanylate cyclase have different kinetics and temperature sensitivities than those of adenylate cyclase, and therefore are probably independent processes.     

The effect of pH, salt concentration and temperature on the survival and growth of Listeria monocytogenes

Factorially designed experiments have been used to study the growth and survival of Listeria monocytogenes in different combinations of pH and salt concentrations at ambient and chill temperatures. Survival at low pH and high salt concentration was strongly temperature dependent. The minimum pH values that allowed survival after 4 weeks from an initial 10(4) cells were 4.66 at 30 degrees C, 4.36 at 10 degrees C and 4.19 at 5 degrees C. These limits were salt dependent, low (4-6%) salt concentrations improved and higher concentrations reduced survival at limiting pH values. The lowest pH that allowed a 100-fold increase in cell numbers within 60 d was 4.66 at 30 degrees C but this was increased to 4.83 at 10 degrees C. At 5 degrees C growth occurred at pH 7.0 but not at pH 5.13. Simple predictive models describing the effect of hydrogen-ion and salt concentration on the time for at least a 100-fold increase in numbers at 10 degrees C and 30 degrees C were constructed after analysis of the results for a least squares fit to a quadratic model. The interactions between salt and hydrogen-ion concentration on growth were found to be purely additive.     

Functional and structural response of a cellulose-degrading methanogenic microbial community to multiple aeration stress at two different temperatures

Two cellulose-fermenting methanogenic enrichment cultures originating from rice soil, one at 15 degrees C with Methanosaeta and the other at 30 degrees C with Methanosarcina as the dominant acetoclastic methanogen, both degraded cellulose anaerobically via propionate, acetate and H2 to CH4. The degradation was a two-stage process, with CH4 production mainly from H2/CO2 and accumulation of acetate and propionate during the first, and methanogenic consumption of acetate during the second stage. Aeration stress of 12, 24, 36 and 76 h duration was applied to these microbial communities during both stages of cellulose degradation. The longer the aeration stress, the stronger the inhibition of CH4 production at both 30 degrees C and 15 degrees C. The 72 h stressed culture at 30 degrees C did not fully recover. Aeration stress at 30 degrees C exerted a more pronounced effect, but lasted for a shorter time than that at 15 degrees C. The aeration stress was especially effective during the second stage of fermentation, when consumption of acetate (and to a lesser extent propionate) was also increasingly inhibited as the duration of the stress increased. The patterns of CH4 production and metabolite accumulation were consistent with changes observed in the methanogenic archaeal community structure. Fluorescence in situ hybridization showed that the total microbial community at the beginning consisted of about 4% and 10% archaea, which increased to about 50% and 30% during the second stage of cellulose degradation at 30 degrees C and 15 degrees C respectively. Methanosarcina and Methanosaeta species became the dominant archaea at 30 degrees C and 15 degrees C respectively. The first round of aeration stress mainly reduced the non-Methanosarcina archaea (30 degrees C) and the non-Methanosaeta archaea (15 degrees C). Aeration stress also retarded the growth of Methanosarcina and Methanosaeta at 30 degrees C and 15 degrees C respectively. The longer the stress, the lower was the percentage of Methanosarcina cells to total microbial cells after the first stress at 30 degrees C. A later aeration stress decreased the population of Methanosarcina (at 30 degrees C) in relation to the duration of stress, so that non-Methanosarcina archaea became dominant. Hence, aeration stress affected the acetotrophic methanogens more than the hydrogenotrophic ones, thus explaining the metabolism of the intermediates of cellulose degradation under the different incubation conditions.     

Temperature and meal size effects on the postprandial metabolism and energetics in a boid snake

We investigated the combined effect of meal size and temperature on the aerobic metabolism and energetics of digestion in Boa constrictor amarali. Oxygen uptake rates (Vd2;o2) and the duration of the digestion were determined in snakes fed with meals equaling to 5%, 10%, 20%, and 40% of the snake's body mass at 25 degrees and 30 degrees C. The maximum Vd2;o2 values attained during digestion were greater at 30 degrees C than at 25 degrees C. Both maximal Vd2;o2 values and the duration of the specific dynamic action (SDA) were attained sooner at 30 degrees C than at 25 degrees C. Therefore, the temperature effect on digestion in Boa is characterized by the shortening of the SDA duration at the expense of increased Vd2;o2. Energy allocated to SDA was not affected by meal size but was greater at 25 degrees C compared to 30 degrees C. This indicates that a postprandial thermophilic response can be advantageous not only by decreasing the duration of digestion but also by improving digestive efficiency. Maximal Vd2;o2 and SDA duration increased with meal size at both temperatures.     

Active knee joint velocity replication measures are stable and accurate in healthy individuals

The purpose of this study was to determine the stability and accuracy of active knee joint velocity replication methods in healthy subjects. We used a repeated measures design with 14 healthy volunteers. Measures of velocity replication were performed in two ranges of knee joint flexion (0 degrees -30 degrees and 60 degrees -90 degrees ), across four testing velocities (5, 10, 15, and 30 degrees /s) in two movement directions (flexion and extension). Statistical analysis included intraclass correlation coefficients (ICCs; 2, k) and associated standard error of the measures calculated between day 1 and 2. We performed z-tests between all possible combinations of ICC pairs using Fisher's Z transformations to determine if any significant differences existed between observed ICCs. We also calculated correlation ratios (eta2) to explain the source of variability in the calculated ICCs. To assess measurement accuracy, we calculated constant error and absolute error between criterion and replication velocities. Results on ICCs and standard error of the measurements (SEMs) ranged from r = -0.44 +/- 7.00 to 0.88 +/- 0.72 degrees /s. Calculated z-tests indicated six paired ICCs were significantly different ( p < 0.1). In all six pairs, the faster test velocity had a lower ICC magnitude. The eta2 calculations demonstrated that inconsistent performance between day 1 and 2 caused the low ICC magnitudes observed with faster testing velocities. Significantly more absolute error occurred at 30 and 15 degrees /s compared with 5 degrees /s. Significantly less constant error was observed for 30 degrees /s compared with 15 degrees /s. A significant direction by range of motion interaction indicated less constant error for flexion movements in the 60 degrees -90 degrees range of motion (ROM) as compared with extension movements in either ROM. Healthy individuals could actively replicate slower criterion velocities in the mid and end ranges of knee joint motion in both movement directions with an acceptable amount of consistency and accuracy. The data support the use of velocity replication in future investigations on proprioceptive function.