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David Hartman Rudolf Werner 《Biochemical and biophysical research communications》1977,77(4):1445-1451
During the incorporation of α-[32P]-dATP into HeLa cell nuclei some of the [32P]-label enters ribonucleic acid presumably because of intracellular nucleotide turnover. This result makes the conclusion about the existence of an RNA-DNA covalent linkage, based on the transfer of [32P]-label from deoxyribonucleotides to ribonucleotides after alkaline hydrolysis of the nuclei acid, untenable. 相似文献
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A progesterone-binding component is reported in the cerebral hemispheres of immature female rat. [3H] progesterone binding in the brain cytosol is increased following two weeks of estradiol administration. The [3H] progesterone binding by this component can be reduced by pretreatment with unlabeled steroid. In addition, the binder from both control and estradiol-treated groups shows inter-action with ATP immobilized on columns of ATP-Sepharose. 相似文献
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木聚糖是一种在自然界中含量仅次于纤维素的丰富的可再生资源,木聚糖酶是一类可以将木聚糖水解成单糖和寡糖的酶,利用木聚糖酶将木聚糖分解后的产物被广泛应用于食品、造纸以及纺织等行业。木聚糖酶按其对酸碱环境的耐受能力分为碱性木聚糖酶、中性木聚糖酶和酸性木聚糖酶,其中碱性木聚糖酶适合应用于造纸工业中,尤其在造纸的制浆、促进漂白及废纸脱墨等多种工艺中,可以显著提高纸张质量,有效降低氯气排放量,从而减少对环境的污染。随着生物技术的进步,利用基因工程技术可以对碱性木聚糖酶进行分子改造,以提高其耐碱、耐热能力,扩大其在工业应用中的条件范围。介绍碱性木聚糖酶在分子改造方面的研究进展以及其在造纸漂白和制浆、废纸脱墨中的应用。 相似文献
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