共查询到19条相似文献,搜索用时 375 毫秒
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TDZ:一种有效的植物生长调节剂 总被引:64,自引:2,他引:62
人工合成的苯基脲衍生物TDZ(N—苯基—N′—1,2,3—噻二唑—5—脲)是已被广泛用于植物组织培养形态发生的高效生物调节剂。它能诱导外植体从愈伤组织形成到体细胞胚胎发生的一系列不同反应,具有生长素和细胞分裂素双重作用的特殊功能。近年来通过研究TDZ启动的形态发生事件,人们正逐渐揭示出其内在作用机理。许多研究报告指出TDZ通过调节内源植物生长激素起作用,或者是诱导逆境产生起间接作用。它还能调节细胞膜结构、能量水平、营养吸收和同化作用。本文将探讨TDZ几种可能的作用机理,并概述近年来有关TDZ诱导的植物离体形态发生效应研究进展。 相似文献
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三种植物生长调节剂对密叶绢藓(Entodon challengeri)孢子萌发、原丝体发育及芽体发生的影响 总被引:3,自引:0,他引:3
研究了3种植物生长调节剂苯基噻二唑基脲(TDZ)、6-苄基腺嘌呤(6-BA)、萘乙酸(NAA)对密叶绢藓[Entodon challengeri(Paris)Cardot]孢子萌发、原丝体发育及芽体发生的影响,并对整个发育过程进行了显微观察和照相,结果表明:(1)3种植物生长调节剂对密叶绢藓孢子萌发影响不显著;(2)在原丝体发育阶段,1.0 mg/LNAA对原丝体初期的发育促进效果显著,0.4 mg/L TDZ对原丝体发育中期分枝的形成促进效果显著,6-BA处理效果不显著;(3)3种植物生长调节剂单独处理均促进芽体的发生,但0.4 mg/L TDZ效果最佳。而1.5 mg/L 6-BA TDZ组合处理效果更加显著;(4)芽体的发生数量与芽体的长势无正相关性。 相似文献
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研究了3种植物生长调节剂苯基噻二唑基脲(TDZ)、6-苄基腺嘌呤(6-BA)、萘乙酸(NAA)对密叶绢藓[Entodon challengeri(Paris)Cardot]孢子萌发、原丝体发育及芽体发生的影响,并对整个发育过程进行了显微观察和照相,结果表明:(1)3种植物生长调节剂对密叶绢藓孢子萌发影响不显著;(2)在原丝体发育阶段,1.0 mg/LNAA对原丝体初期的发育促进效果显著,0.4 mg/L TDZ对原丝体发育中期分枝的形成促进效果显著,6-BA处理效果不显著;(3)3种植物生长调节剂单独处理均促进芽体的发生,但0.4 mg/L TDZ效果最佳。而1.5 mg/L 6-BA+TDZ组合处理效果更加显著;(4)芽体的发生数量与芽体的长势无正相关性。 相似文献
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棉花脱叶剂TDZ简介 总被引:16,自引:1,他引:15
TDZ(Thidiazuron)是西德Schering公司1976年宣布合成的新植物生长调节剂,化学名为N-苯基-N′-1,2,3-噻二唑-5-脲(N-phenyl-N′-1,2,3-thiadiazol-5-ylurea),结构式是: 相似文献
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复叶槭顶芽组织培养再生体系的建立 总被引:1,自引:0,他引:1
初步建立了复叶槭顶芽组培再生体系。N-苯基-N'-1,2,3-噻二唑-5-脲(TDZ)可诱导复叶槭顶芽分化。复叶槭顶芽的最佳增殖培养基为MS 0.1mg·L-16-BA 0.01mg·L-1NAA,最佳分化培养基为MS 0.01mg·L-1TDZ,最佳生根培养基为MS 0.1mg·L-1NAA。 相似文献
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B. N. S. Murthy S. J. Murch Praveen K. Saxena 《In vitro cellular & developmental biology. Plant》1998,34(4):267-275
Summary TDZ (N-phenyl-N’-1,2,3-thidiazol-5-ylurea) is a substituted phenylurea compound which was developed for mechanized harvesting of cotton bolls
and has now emerged as a highly efficacious bioregulant of morphogenesis in the tissue culture of many plant species. Application
of TDZ induces a diverse array of cultural responses ranging from induction of callus to formation of somatic embryos. TDZ
exhibits the unique property of mimicking both auxin and cytokinin effects on growth and differentiation of cultured explants,
although structurally it is different from either auxins or purine-based cytokinins. A number of physiological and biochemical
events in cells are likely to be influenced by TDZ, but these may or may not be directly related to the induction of morphogenic
responses, and hence, the mode of action of TDZ is unknown. However, the recent approaches applied to study the morphogenic
events initiated by TDZ are clearly beginning to reveal the details of a variety of underlying mechanisms. Various reports
indicate that TDZ may act through modulation of the endogenous plant growth regulators, either directly or as a result of
induced stress. The other possibilities include the modification in cell membranes, energy levels, nutrient uptake, or nutrient
assimilation. In this review, several of these possiblities are presented and discussed in light of recently published studies
on characterization of TDZ-induced morphogenic effects. 相似文献
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Effects of chemical substances on the rapid cultivation of moss crusts in a phytotron from the Loess Plateau,China 总被引:1,自引:0,他引:1
Yongsheng Yang Li Zhang Xingfang Chen Wen Wang Yingnian Li 《International journal of phytoremediation》2019,21(3):268-278
In this study, typical moss crusts, which were dominated by the species Didymodon vinealis (Brid.) Zand., were collected from the Loess Plateau and a 65-day cultivation experiment was performed to study the effects of five kinds of nutrient solutions (Knop, Murashige-Skoog (MS), Benecke, Part and Hoagland), two kinds of carbohydrates (glucose and sucrose) and three kinds of plant growth regulators (thidiazuron (TDZ), 6-benzylaminopurine (6-BA) and naphthaleneacetic acid (NAA)) on the coverage, plant density, and plant height of moss crusts. The main conclusions are as follows. (1) All Knop, MS, Benecke, Part and Hoagland nutrient solutions improved the coverage and plant density of moss crusts to different degrees and the promotional effects of the Hoagland nutrient solution were most significant. (2) Glucose and sucrose could promote the formation of moss crusts, but they inhibited the development of moss crusts at concentrations greater than 10?g/L. (3) With an increase in concentration, the effects of TDZ on the development of moss crusts changed from “enhanced” to “inhibited”. Regardless of whether the concentration was high or low, 6BA had no significant effects on the growth of moss crusts, and NAA reduced the development of moss crusts. Results suggest that nutrient solutions (e.g. Hoagland), low concentration carbohydrates solutions, and some plant growth regulators (e.g. 1?mg/L TDZ) enhance the development of moss crusts in Loess Plateau under the appropriate environmental conditions. 相似文献
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目的:研究苯基噻二唑基脲(Thidiazuron,TDZ)对喜树愈伤组织生长及叶绿素、花青素积累的影响。方法:采用TDZ单独应用及合并植物激素对喜树愈伤组织进行了继代培养。结果:单独应用TDZ时1mg/L为组织生长最适浓度。1mg/L TDZ+0.5mg/L NAA可显著促进愈伤组织的生长及叶绿素的积累,1mg/L TDZ+0.5mg/L 2,4-D培养条件下花青素含量高于单独应用TDZ培养。结论:TDZ在喜树愈伤组织培养中可替代植物激素并对细胞植物色素积累有明显影响。 相似文献
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Nam Hee Yoo Ok Tae Kim Jung Bong Kim Sun Hee Kim Young Chang Kim Kyong Hwan Bang Dong Yun Hyun Seon Woo Cha Min Young Kim Baik Hwang 《Plant biotechnology reports》2011,5(3):283-287
In order to produce centellosides from whole plant cultures of Centella asiatica (L.) Urban, we evaluated the synergistic effects of thidiazuron (TDZ) and methyl jasmonate (MJ) on whole plant growth and
centelloside production. After 4 weeks of treatment with 0.025 mg/L of TDZ coupled with 0.1 mM MJ, the production of madecassoside
and asiaticoside from whole plant cultures was estimated to be 2.40- and 2.44-fold, respectively, above that of MJ elicitation
alone. When whole plants were treated with a growth regulator and an elicitor, the growth of whole plants, as compared to
the controls, did not differ. Additionally, total phytosyterol content in the leaves of whole plants co-treated with MJ and
TDZ was 1.08-fold greater than those of MJ alone. These results demonstrate that combined treatments not only stimulate the
accumulation of centellosides in the leaves but also inhibit the reduction of phytosterol levels caused by MJ elicitation. 相似文献
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匙羹藤组织培养条件优化研究 总被引:3,自引:1,他引:2
以匙羹藤无菌种子苗为外植体,通过预备实验确定基本培养基后,采用完全实验和正交设计相结合的方法,研究植物生长调节剂两因素配合和多因素组合(NAA、TDZ、2-ip、IAA、6-BA和KT)对匙羹藤芽体诱导及继代培养的影响。结果显示,匙羹藤组织培养最适的基本培养基为N68;N68+NAA0.05mg/L+2-ip0.4mg/L+TDZ0.001mg/L利于芽体增殖,可用于继代培养;N68+NAA0.05mg/L+TDZ0.005mg/L促进植株长根,可用于生根培养。 相似文献
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The effects of thidiazuron (TDZ) on adventitious bud and shoot formation from hypocotyl segments of sweetgum (Liquidambar styracifiua) were tested alone and in combination with 2,4-dichlorophenoxyacetic acid (2,4-D). The combination of 1 mg/1 TDZ with 0.01 mg/l 2,4-D resulted in the highest frequency of bud production. Lower concentrations of TDZ stimulated shoot production, generating the most shoots at 0.1 mg/1 TDZ with 0.01 mg/1 of 2,4-D. Inhibition of shoot elongation by TDZ was overcome by transferring shoot cultures to a shoot proliferation medium lacking TDZ or containing naphthaleneacetic acid and benzyladenine in addition to TDZ. Shoot production in liquid culture was significantly greater than that in solid culture. Comparisons of in vitro and ex vitro rooting of the adventitious shoots demonstrated that ex vitro rooting produced plants with faster growth rates and more extensive root systems.Abbreviations
BA
Benzyladenine
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IBA
indole-3-butyric acid
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NAA
naphthaleneacetic acid
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PGR
plant growth regulator
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TDZ
thidiazuron
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2,4-D
2,4-dichlorophenoxyacetic acid 相似文献
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C. G. Zhang W. Li Y. F. Mao D. L. Zhao W. Dong G. Q. Guo 《Russian Journal of Plant Physiology》2005,52(3):345-351
Callusing was induced from three kinds of explants of Chinese medical plant Scutellaria baicalensis with thidiazuron (TDZ) as an exogenous growth regulator. Multiple shoots were formed from these calli on hormone-free media. Calli induced by 0.3 mg/l TDZ produced shoots directly on the induction medium after culturing for 30–40 days without any interval, while at a higher concentrations of TDZ (1.0, 2.0, and 5.0 mg/l), regeneration occurred only after transfer to hormone-free medium. Endogenous hormones were detected by reverse-phase HPLC-MS and ELISA. The higher TDZ concentrations decreased endogenous benzyladenine (BA) and IAA levels, while iPA and ZR levels and their ratios to IAA were not correlated with TDZ concentrations, growth of calli, or plant regeneration. BA was the dominant cytokinin during calli growth and bud formation, 10 000-fold more abundant than iPA and ZR. Moreover, low IAA/BA ratio, rather than low IAA or high cytokinin levels alone, is the most important factor for shoot formation by calli. TDZ might act indirectly by changing endogenous hormonal levels, most likely the IAA/BA ratio which controls the calli growth and bud formation.__________From Fiziologiya Rastenii, Vol. 52, No. 3, 2005, pp. 392–398.Original English Text Copyright © 2005 by Zhang, Li, Mao, Zhao, Dong, Guo.This article was submitted by the authors in English. 相似文献
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Waraporn Chouychai Maleeya Kruatrachue Hung Lee 《International journal of phytoremediation》2015,17(11):1053-1059
The influence of three plant growth regulators, indolebutyric acid (IBA), thidiazuron (TDZ) and gibberellic acid (GA3), either individually or in pair-wise combinations, on the ability of waxy corn plant to remove hexachlorocyclohexane (HCH) from contaminated soil was studied. Waxy corn seeds were immersed for 3 h in solutions of 1.0 mg/l IBA, 0.01 mg/l TDZ, 0.1 mg/l GA3, or a mixture of two of the growth regulators, and then inoculated in soil contaminated with 46.8 mg/kg HCH for 30 days. Pretreatment of corn seeds with the plant growth regulators did not enhance corn growth when compared with those immersed in distilled water (control), but the pretreatment enhanced HCH removal significantly. On day 30, HCH concentration in the bulk soil planted with corn seeds pretreated with GA3 or TDZ+GA3 decreased by 97.4% and 98.4%, respectively. In comparison, HCH removal in soil planted with non-pretreated control waxy corn seeds was only 35.7%. The effect of several growth regulator application methods was tested with 0.01 mg/l TDZ. The results showed that none of the methods, which ranged from seed immersion, watering in soil, or spraying on shoots, affected HCH removal from soil. However, the method of applying the growth regulators may affect corn growth. Watering the corn plant with TDZ in soil led to higher root fresh weight (2.2 g) and higher root dried weight (0.57 g) than the other treatments (0.2–1.7 g root fresh weight and 0.02–0.43 g root dried weight) on day 30. Varying the concentrations of GA3 did not affect the enhancement of corn growth and HCH removal on day 30. The results showed that plant growth regulators may have potential for use to enhance HCH phytoremediation. 相似文献
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A. Sieboldianus (5-leaf aralia) is recalcitrant for micropropagation, but has very good landscaping potential. This research was conducted
with the following objectives: (1) to study effects of BA, TDZ, CPPU, 2iP, kinetin and zeatin in woody plant medium on the
performance of softwood shoot nodal explants produced by field grown 5-leaf aralia plants; (2) to investigate influences of
BA or TDZ in the forcing solution on subsequentin vitro shoot initiation of nodal explants taken from forced softwood growth. Shoot initiation of softwood nodal explants from field-grown
plants was promoted by adding BA, TDZ or CPPU to the culture medium. Kinetin, zeatin and 2iP were ineffective for micropropagation
ofA. Sieboldianus. The forced softwood growth for use as explants was “primed” by forcing dormant stems in solution containing 200 mg 8-HQC
per liter plus 2% sucrose, 44.4, 222, or 444 μM BA, or 45.4, 227, or 454 μM TDZ. BA and TDZ in the forcing solution enhanced
subsequentin vitro axillary shoot initiation of nodal explants taken from forced stems by doubling the number of shoots produced per explant
to 3.3 from 1.65 shoots per explant taken from field grown plants. This forcing solution technique also reduced the time needed
from culture initiation to potted plants to half of the time needed for the conventional micropropagation method (12 to 14
vs. 25 to 27 weeks), thus expediting the micropropagation ofA. Sieboldianus. 相似文献