旋转恒定磁场对大鼠血清中铁代谢相关蛋白的影响

目的:观察旋转恒定磁场对大鼠血清中铁代谢相关蛋白的影响,为深入认识磁场的多方面生物学效应提供依据.方法:将SD大鼠随机分为对照组和曝磁组.后者在磁场强度是0.4 T、频率为8.97 Hz的环境中每天曝磁1次,每次2 h,连续处理30 d后处死分组大鼠,获得血清,冻存后测定血清中铜蓝蛋白活力和总抗氧化能力、铁蛋白和乳铁蛋白含量等指标.结果:曝磁组大鼠血清中铜蓝蛋白活力与对照组大鼠相比没有显著性变化;总抗氧化能力有了显著性的提高(P＜0.05);铁蛋白含量有显著性的降低(P＜0.05);乳铁蛋白含量有显著性的降低(P＜0.01).结论:磁场对大鼠血清中铁代谢的部分相关蛋白存在影响.     

不同磁场对大鼠脏器一氧化氮含量的影响

目的 :探讨磁场对大鼠肝组织、肾组织中一氧化氮 (NO)含量的影响。方法 :将大鼠置于不同磁场 ( 1 0mT、2 0mT、30mT)中曝磁 30min ,观察磁场对大鼠肝组织和肾组织NO含量的影响。NO含量的测定采用改良的Griess法。结果 :在 30mT磁场中曝磁 30min ,大鼠肝组织、肾组织中NO含量都显著高于对照组(p <0 .0 1 ) ;其它场强NO含量与对照组相比无统计学意义。结论 :30mT磁场对大鼠肝组织、肾组织NO含量有一定影响。     

穴位埋磁对家兔实验性动脉粥样硬化的影响

目的 :探讨穴位埋磁对家免实验性动脉硬化的影响 ,为磁场防治动脉粥样硬化提供依据。方法 :随机将实验家免分为三组。喂高脂饲料的家兔 ,在其足三里 (双侧 )、内关 (双侧 )及关元穴位埋磁 90天 ,检测其血清胆固醇 (TC)、低密度脂蛋白 (LDL -c)、甘油三酯 (TG)水平。结果 :检测结果均明显低于对照组 (p <0 .0 1 ) ,高密度脂蛋白 (HDL -c)水平显著高于对照组 (p <0 .0 1 )。主动脉内膜脂纹及粥样灶面积亦均显著小于对照组 (p <0 .0 1 )。结论 :穴位埋磁具有减轻和防治动脉硬化的功效。     

磁场作用对小鼠学习记忆的影响

目的 :探讨磁场作用对小鼠学习记忆能力的影响。方法 :应用水迷宫学习模型测定并对比 30分钟磁场处理组、1 5分钟磁场处理组和非磁场处理的正常对照组动物的空间学习记忆能力。结果 :水迷宫学习训练的实验表明 30分钟磁场处理组与正常对照组比较 ,动物到达水下平台的时间延长 ;游程增加 ;平均游速减慢 ,且均具有显著性差异 (p <0 .0 5)。 1 5分钟磁场处理组与正常对照组比较 ,动物到达水下平台的时间延长 ,且具有显著性差异 (p <0 .0 5) ;游程和平均速度与正常对照组相比无显著性差异 (p >0 .0 5)。结论 :磁场处理 30分钟或 1 5分钟损伤小鼠的空间学习记忆能力 ,且以 30分钟的磁场处理作用较强     

磁处理党参药液对小鼠肠推进运动影响的药效研究

目的 :观察磁处理党参药液对小鼠肠推进运动的影响。方法 :实验分为非磁处理党参药液对照组 ,生理盐水对照组 ,0 .1T磁处理试验组和 0 .2 5T磁处理药液试验组 ,采用灌胃给药的途径进行实验观察。结果 :0 .1T和 0 .2 5T磁处理药液组和生理盐水对照组相比较 ,试验组具有显著促进小鼠肠的炭末推进作用 (p <0 .0 1 ) ;非磁处理与磁处理党参药液组相比较 ,0 .1T和 0 .2 5T磁处理药液组对小肠的炭末推进率有着显著的抑制作用 (p <0 .0 1 )。结论 :党参磁处理药液对小鼠肠的推进有明显作用。     

磁处理党参药液对小白鼠碳粒廓清功能影响的研究

目的 :观察小白鼠饮用磁处理党参药液后对碳粒廓清功能的影响。方法 :用碳粒廓清法比较正常对照组、非磁处理党参药液组与磁处理党参药液组对小白鼠碳粒廓清功能的影响。结果 :与正常对照组比较 ,磁处理党参药液组光密度 (OD)降低 ,差异极显著 (p <0 .0 1 ) ,廓清指数 (K)都有提高 ,差异显著 (p <0 .0 5 ) ,吞噬指数 (α)也有提高 ,其中 0 .2 5T药液组达差异显著 (p <0 .0 5 ) ;与非磁处理药液组比较 ,光密度(OD)逐渐下降 ,当取血时间为 1 0min时 ,0 .2 5T药液组达差异显著 (p <0 .0 5 ) ,廓清指数 (K) ,0 .2 5T药液组有提高 ,但差异不显著 ,吞噬指数 (α)都有提高 ,仅 0 .2 5T药液组达差异显著 (p <0 .0 5 )。结论 :磁处理党参药液具有促进单核巨噬细胞系统吞噬功能的作用 ,对免疫有良好作用。     

磁处理党参药液对小鼠血液生理功能影响的研究

目的:观察小白鼠饮食磁处理党参药液后对血液功能的影响。方法:用血球计数仪测定白细胞数(WBC)、红细胞数(RBC)、血红蛋白浓度(HGB)及血小板数(PLT) ,采用比较法比较正常对照组、非磁处理党参药液组与磁处理党参药液组对小白鼠血液功能的影响。结果:与正常对照组比较,磁处理党参药液组白细胞数差异不显著(p >0 .0 5 ) ,红细胞数、血红蛋白浓度显著提高,差异极显著(p <0 .0 1) ,血小板数0 .2 5T药液组达差异显著(p <0 .0 5 ) ,0 .1T药液组差异不显著;与非磁处理药液组比较,磁处理党参药液组白细胞数差异不显著(p >0 .0 5 ) ,而红细胞数、血红蛋白浓度及0 .2 5T血小板数均有提高,差异极显著(p <0 .0 1) ,0 .1T药液组血小板数差异不显著(p >0 .0 5 )。结论:磁处理党参药液对血细胞数变化有明显作用。     

旋转恒定磁场对类风湿关节炎大鼠血清铁的影响

目的:以类风湿关节炎SD大鼠为模型,观察旋转恒定磁场对类风湿关节(rheumatoid arthritis,RA)模型大鼠的治疗效果及对血清铁的影响。方法:用乳化剂天然牛胶原蛋白Ⅱ加完全弗氏佐剂法建立RA大鼠模型。将RA大鼠随机分为对照组、磁场强度是0.4T,频率分别为6.72Hz和8.97Hz的曝磁组,每天曝磁两次,每次2 h,曝磁过程中每天观察大鼠关节肿胀程度,30 d后测定血清铁状态及转铁蛋白和转铁蛋白受体含量。结果:曝磁组RA大鼠关节的肿胀程度显著减小,由严重肿胀且不能负重变为中度肿胀或轻度肿胀,且血清中转铁蛋白和转铁蛋白受体含量有显著的增加,血清铁升高。结论:磁场对类风湿关节炎大鼠有治疗作用,对RA大鼠铁吸收有促进作用。     

磁处理党参、白术药液对胃蛋白酶活性影响的研究

目的 :观察磁处理党参、白术药液对胃蛋白酶活性的影响。方法 :用麦特 (Mett)氏法比较正常对照组 ,非磁处理党参、白术药液组与磁处理党参、白术药液组的胃蛋白酶活性。结果 :与正常对照组比较 ,磁处理党参、白术药液组有显著提高胃蛋白酶活性的作用 (p <0 .0 1 ) ;与非磁处理药液组比较 ,磁处理药液组也都有提高胃蛋白酶活性的作用 (p <0 .0 5及p <0 .0 1 )。结论 :磁处理党参、白术药液可提高胃蛋白酶活性     

磁场处理离体小肠对葡萄糖吸收功能的影响

目的 :观察磁场处理离体小肠对葡萄糖吸收功能的影响。方法 :用经 0 .1T和 0 .2 5T磁场处理小白鼠离体小肠 ,分别测定其对葡萄糖的吸收值。结果 :用 0 .1T和 0 .2 5T磁场处理的小白鼠离体小肠浆膜侧吸收葡萄糖的量与对照组比较 ,0 .1T差异显著 (p <0 .0 5 ) ,0 .2 5T的差异极显著 (p <0 .0 1 )。结论 :用0 .1T和 0 .2 5T磁场处理的小白鼠离体小肠对葡萄糖的吸收均有明显的促进作用。     

Copper and zinc concentrations and the activities of ceruloplasmin and superoxide dismutase in atherosclerosis obliterans

The relationships among concentrations of copper and zinc, the oxidase activity of ceruloplasmin (Cp) in serum, and Cu,Zn-SOD (superoxide dismutase) activity in erythrocytes were investigated in men with atherosclerosis obliterans (AO) and a control group. The oxidase activity of Cp was measured with o-dianisidine dihydrochloride as a substrate, and Cu,Zn-SOD activity in erythrocytes by using the RANSOD kit. The lipid profile and uric acid concentration were determined in AO and control groups. The results showed higher copper and zinc concentrations in serum in the AO group (20.0±3.5 and 18.0±3.2 μmol/L, respectively) in comparison with the control group (15.6±2.3 and 14.7±1.9 μmol/L). The Cp activity in serum was higher in the AO group (174.2±61.8 U/L) than in the control group (93.7±33.9 U/L), and a significant difference was found in the activity of Cu,Zn-SOD in erythrocytes (2389±1396 and 1245±365 U/g Hb, respectively) between both groups. The activity of Cu,Zn-SOD was positively correlated with copper in the control group (r=0.73), but not in AO, and negatively with uric acid concentration (r=−0.63) in the AO group. The oxidase activity of Cp was correlated with copper, but not zinc, in AO and control groups (r≥0.65). Negative correlation coefficients were calculated for uric acid and copper and zinc concentrations in the AO group (−r≥0.61). Increased copper concentrations and oxidase activity of Cp in serum in AO and the activity of Cu,Zn-SOD in erythrocytes could result from atherosclerotic disease, accompanied by chronic ischemia of a lower limb. These results suggest also that relationship between copper concentration and Cu,Zn-SOD activity in erythrocytes found in the serum of healthy subjects may be disturbed in pathologic conditions.     

Immunochemical and enzymatic study of ceruloplasmin in rheumatoid arthritis

Forty adult patients (30 women and 10 men) with rheumatoid arthritis (RA), treated with nonsteroidal anti-inflammatory drugs, were studied. Serum levels of immunoreactive ceruloplasmin, oxidase activity of the ceruloplasmin and total copper, as well as the specific oxidase activity (enzyme activity per unit of mass) and the copper/immunoreactive ceruloplasmin relationship were significantly higher in the group of patients than in the healthy control group (p < 0.001). However, no significant difference was found for the concentration of non-ceruloplasmin copper between both groups. A statistically significant negative correlation was obtained for the concentration of serum thiobarbituric acid-reacting substances with the immunoreactive ceruloplasmin and its oxidase activity in the group of patients (p < 0.005). These results suggest that in RA increases of serum copper are produced at the expense of the fraction linked to the ceruloplasmin, diminishing the proportion of apoceruloplasmin and other forms poor in copper. Although the increase in the serum concentration of ceruloplasmin might offer an additional safeguard against oxidative stress. it does not appear to have a beneficial effect upon the activity of the illness as evaluated by means the biological inflammation markers C-reactive protein, erythrocyte sedimentation rate and sialic acid.     

The effect of 110mAg on ceruloplasmin oxidase activity in rats

The effect of single i.v. injection of 110mAgNO3 (0.183 mg Ag+ X kg-1 b.wt.) in rats on the ceruloplasmin oxidase activity (Cp) and copper serum concentration was studied. It was found that Cp activity in the serum decreased to 70% of the control value and simultaneously serum copper concentration decrease to 30% of the control level. In both cases the decrease was independent on the time elapsed after silver administration. Comparing these results with those reported recently in mice Cu deficit in the rat serum was approximately twice higher. This fact is considered to be an inter-species difference. The concentration of copper in the hepatic supernatant significantly decreased (to eight times from control value) after silver injection. Only less than 10% of the total amount of Ag found in whole liver was taken up to hepatic supernatant. GPC analysis of the supernatant (Sephadex G-75) revealed that no Ag-metallothionein fraction is present. On the basis of the results obtained it was concluded that the mechanism of silver inhibition of Cp oxidase activity remains still in question.     

Variations in Copper Concentration and Ceruloplasmin Activity of Dairy Cows in Relation to Lactation Stages with Regard to Ceruloplasmin to Copper Ratios

Variations of copper (Cu) concentration and ceruloplasmin (Cp) activity in serum and plasma of dairy cows at different stages of lactation were assessed in 240 Holstein dairy cows. Furthermore, ceruloplasmin to copper ratios were also investigated. The cows were classified according to their lactation stages into four different groups as close-up stage (3–1 week(s) antepartum), fresh lactation stage (0–1 week postpartum), early lactation stage (3–5 weeks postpartum), and mid-lactation stage (15–18 weeks postpartum). Each group consisted of 60 multiparous cows. Serum and heparinised plasma samples were obtained from each cow. Concentrations of serum copper (sCu) and plasma copper (pCu) as well as activities of plasma Cp (pCp) were higher in the group of fresh lactation stage than other groups (P < 0.05). Serum Cp (sCp) exhibited no significant difference between fresh lactation and early lactation groups (P > 0.05). Low correlations were obtained between sCp and pCu, sCp and sCu, and sCu and pCu. Plasma copper concentration and plasma ceruloplasmin activity were higher than serum copper concentration and serum ceruloplasmin activity, respectively (P < 0.001). The ratios of Cp activity to Cu concentration (Cp/Cu) were not significantly changed in the different lactation stages of dairy cows (P > 0.05). Use of sCp/pCu and sCp/sCu rather than pCp/pCu will reduce the calculated value of Cp/Cu. Furthermore, for evaluation of copper status, use of sCp/sCu or sCp/pCu identified more animals as ‘low’ and ‘marginal’ than using pCp/pCu (P < 0.001). It can be concluded that ceruloplasmin and copper undergo a physiological increase just after calving; thus, their values should be interpreted with caution during assessment of copper status. Plasma measurements should be used for calculation of Cp/Cu, and further research is required to refine diagnosis criteria for use of such ratio in determining copper status in dairy cows.     

Serum Copper Homeostasis in Hypertensive Intracerebral Hemorrhage and its Clinical Significance

This study was to investigate the alterations of serum copper homeostasis after hypertensive intracerebral hemorrhage (ICH), which is not yet clear. We recruited 85 hypertensive ICH patients and determined their serum levels of total copper (TCu), small molecule copper (SMC), and ceruloplasmin (Cp). Sera from 32 healthy persons and 12 primary hypertension patients were collected and analyzed as well. Serum TCu levels in ICH patients were tested at three time points (on admission, day 3, and day 7) and found to be higher than that in hypertension patients (p?<?0.05). The serum SMC levels in hypertension patients and ICH patients at three time points were higher than that in healthy controls (p?<?0.05). Higher serum SMC levels on days 3 and 7 were associated with death in the hospital. Additionally, higher serum SMC levels on the seventh day were associated with poor outcome at discharge. High serum Cp levels on admission, as well as low serum Cp levels on the seventh day, were associated with death in the hospital (p?=?0.002 and p?=?0.034, respectively). Our findings indicated that declines in serum Cp and increases in serum SMC are correlated with lethal or poor outcome in hypertensive ICH patients, possibly as a result of contributions to secondary injury of brain after hemorrhage due to impairment of iron transport and enhanced oxidative stress.     

磁场作用对小鼠抗应激能力的影响

目的 :探讨磁场作用对小鼠抗应激能力的影响。方法 :对磁场处理 30分钟和 1 5分钟的两组实验组与非磁场处理的正常对照组进行游泳耐疲劳运动时间的比较。结果 :游泳耐疲劳运动实验表明 30分钟磁场处理组与正常对照组比较 ,动物游泳耐疲劳运动时间延长 ,且具有显著性差异 (p <0 .0 5 ) ;1 5分钟磁场处理组与正常对照组比较 ,动物游泳耐疲劳运动时间无明显差异 (p >0 .0 5 ) ;30分钟磁场处理组与 1 5分钟磁场处理组比较 ,动物游泳耐疲劳运动时间延长 ,且具有显著性差异 (p <0 .0 5 )。结论 :在一段时间( 30天 )内 ,每天给予小鼠 30分钟的磁场处理明显提高了小鼠的抗应激能力 ,而磁场处理 1 5分钟对小鼠的抗应激能力不产生影响。     

Alterations of serum selenium, zinc, copper, and iron concentrations and some related antioxidant enzyme activities in patients with cutaneous leishmaniasis

The aim of this study was to measure the alterations in serum selenium (Se), copper (Cu), zinc (Zn), and iron (Fe) concentrations and their carrier proteins, ceruloplasmin (Cp), transferrin (Tf) albumin, and related antioxidant enzyme activities, erythrocyte Cu-Zn Superoxide dismutase (Cu-Zn SOD), glutathione peroxidase (GSH-Px), and catalase (CAT) activities in patients with cutaneous leishmaniasis (CL). Erythrocyte Cu-Zn SOD activities, serum Cu concentrations, and Cp levels were found to be significantly higher in the patients group than those of controls. However, GSH-Px and CAT activities and Se, Zn, Fe, and Tf levels were lower in patients than in the control subjects. There were positive important correlation’s between Cu-Zn SOD and Cp, Cu-Zn SOD and Cu, Cp and Cu, GSH-Px and Se, and Fe and CAT in the patients group. Our results showed that serum essential trace elements Se, Zn, Cu, and Fe concentrations and their related enzymes Cu-Zn SOD, GSH-Px, and CAT activities change in CL patients. The changes may be a part of defense strategies of organism and are induced by the hormonelike substances.     

Trace elements and some extracellular antioxidant protein levels in serum of patients with laryngeal cancer

In this study, we investigated the levels of serum Zn, Cu, Mg, Mn, Fe, ceruloplasmin (Cp), transferrin (Trf), and albumin in laryngeal carcinoma and correlated their levels with the cancer stage. The sera from 35 patients with laryngeal cancer (10 at stage II, 12 at stage III, and 13 at stage IV) were extracted before treatment and compared with those from the healthy control group (n=15). Although serum Fe and Mn concentrations were lower in the laryngeal cancer groups (for all stages) than in the control group, the difference was not statistically significant (p>0.05). The higher Cu (p<0.001) and Cp (p<0.01) and lower Zn (p<0.01), Mg (p<0.001), and Trf (p<0.01) concentrations were found in laryngeal cancer groups (for each stage) when compared to the control group. In the comparison of stages II, III, and IV (with each other), all parameters were found to be statistically not significant (p>0.05). On the other hand, no meaningful difference was found in terms of the serum albumin level. In our opinion, alterations in the level of trace elements and antioxidant proteins, important for many metabolic processes, in laryngeal cancer may not be a reason for but is, in fact, a consequence of the disease itself.     

Ceruloplasmin, an Indicator of Copper Status

For clinical purposes, the non-ceruloplasmin copper fraction is routinely derived on the basis that ceruloplasmin binds six Cu atoms. However, this approach is limited because the actual ceruloplasmin copper binding is unclear. We performed direct measurement of the total serum copper and ceruloplasmin in 790 healthy individuals. We used an immunoprecipitation technique to separate ceruloplasmin and determined Cu content. With these values, we calculated the Cu/ceruloplasmin (Cp) ratio and thus generated data to support or discard the theoretical calculation of the non-ceruloplasmin fraction. Average of serum Cu and Cp levels were 18.4 +/- 4.4 micromol/l and 390 +/- 100 mg/l, respectively. The immunoprecipitation procedure allowed us to calculate a Cu/Cp ratio of 5.8, respectively, which supports the methodology of calculation that assigns a mean of six copper atoms to each ceruloplasmin molecule. With these values, we calculated that, in apparently normal adults, the non-ceruloplasmin copper (NCPC) fraction is lower than 1.3 micromol/l of Cu. In this report, we examine the Cp/Cu ratio by using Cp immunoprecipitation procedure. Our in vitro and in vivo studies indicate that, as a mean, there are 5.8 atoms of Cu per Cp molecule and that <1.3 micromol/l of Cu would correspond to the NCPC.