中国猕猴桃属植物叶表皮毛微形态特征及数量分类分析

选取国产猕猴桃35个分类群的代表植株,应用光学显微镜对其新鲜叶表皮毛的微形态特征、形体大小、细胞结构、分布和密度等多态性状和数量性状进行了观察和测量。该属植物的叶表皮毛微形态特征可归纳为6个类型：1)单细胞毛;2)单列多细胞毛,包括线形单列毛、泡状单列毛、扭曲毛、直壁单列毛、曲壁单列毛;3)多列渐尖毛和急尖毛,包括多列曲壁渐尖毛和急尖毛、多列直壁渐尖毛和急尖毛; 4)多列粗毛,包括柱状毛、多列渐尖粗毛、多列急尖粗毛;5)星状毛,包括薄壁星状毛、厚壁星状毛(分正常状况和莲座毛、盾形星状毛、叠生星状毛等特殊状况);6)二歧分枝毛。在此基础上,建立了数据矩阵,并选择猕猴桃科藤山柳lematoclethra lasioclada作为外类群,应用Wagner法和UPGMA聚类法对中国猕猴桃属植物进行了分支分析和表征分析。分支分析结果表明：梁畴芬在1984年发表的分类系统中的净果组为单系类群,而其它3个组(斑果组、糙毛组和星毛组)均不构成单系类群。表征分析反映了猕猴桃属分类群间的表征亲缘关系,特别是反映了中华猕猴桃与美味猕猴桃两个物种间关系较近,而与硬齿猕猴桃两个变种间的关系较远。叶表皮毛微形态特征及数量分类分析方法对研究猕猴桃属植物的系统发育关系和亲缘关系具有重要的意义。     

亚洲粗叶木属及相关属植物叶表皮微形态特征

利用光学显微镜和电子扫描显微镜研究了亚洲茜草科粗叶木属植物26种（包括1个亚种和2个变种）和相关4属即巴戟天属、九节属、染木属和尖叶木属6种植物的叶表皮微形态特征。粗叶木属植物上表皮细胞形状一般呈不规则波状、近椭圆形或者不规则多边形,下表皮细胞一般呈不规则皱波状或者多裂深波状,细胞壁曲折无章,细胞间界限不明显,上、下表皮细胞壁均有加厚现象。叶表皮角质层明显,在电镜下有以下几种类型：颗粒状,网状,鳞片状,乳突状。气孔器几乎全部生在下表皮,有些种气孔下陷,气孔器一般是一对保卫细胞和一对副卫细胞平列。一些粗叶木属植物种叶上着生表皮毛。表皮毛分为单细胞毛和单列多细胞毛两种结构类型以及扭曲毛,线形毛和直壁毛3种形态类型。相关属的叶表皮微形态特征与粗叶木属植物较为相似。粗叶木属植物叶表皮微形态特征具有一定的分类学价值,其性状虽有遗传稳定性,但随生态环境不同而有一定变异。粗叶木属植物叶气孔长轴径/径轴变化幅度比较大以及叶表皮上的毛被多样化均能反映其对生态环境的适应变化。     

中国蒿属植物比较形态和解剖学研究——(Ⅰ)叶表皮结构

本文通过光学显微镜和扫描电镜,观察国产菊科蒿属2亚属7组30种代表植物成熟叶片的表皮细胞、气孔器、表皮毛的特征和气孔形状、气孔外拱盖及拱盖内缘、角质膜和蜡质纹饰。其中表皮细胞多角(边)形,大小不等,垂周壁平直、弓形,或者表皮细胞形状不规则,大小不等,垂周壁波浪状或波纹状。下表皮均有气孔器,上表皮有或无气孔器,气孔器类型有无规则型。无规则四细胞型、不等细胞型、十字型、横列型和平列型。大多数种类具多细胞单列毛。多细胞双列毛和腺毛仅发现在腺毛蒿组。这些微形态特征在组间存在着某些差异,具有一定的分类学和生态学意义。     

广东省淡水红藻弯枝藻属一种（Compsopogon sp．）的形态描述及分类地位探讨

2009年8月在广东省龙川县定南水河采集到一份弯枝藻属的标本,其形态特征介于弯枝藻（Compsopogon caeruleus）和疏枝弯枝藻（Csparsus）之间。植物体株高10～18cm,主轴直径180～270μm,微收缢;中轴细胞近球形和扁球形,横壁收缢明显,直径150-200μm,高100～110μm;皮层细胞1层,中轴细胞缢缩处为2—3层;多角形或卵形,长25~35μm,直径20-25μm;分枝直出至侧生,某些基部细胞特化为假根;分枝顶端渐尖、急尖或二叉状;单孢子球形,直径10—14μm。该标本可能为弯枝藻和疏枝弯枝藻在演化过程中的一种过渡类型。     

栎属青冈亚属（壳斗科）的叶表皮研究

利用光镜和扫描电镜观察了栎属青冈亚属Quercus subgen. Cyclobalanopsis 48种植物的叶表皮,尤以对叶下表皮的毛被特征观察较为仔细。共观察到8种不同类型的叶表皮毛：单列毛、单毛、乳突、星状毛、溶和星状毛、具柄束毛、多出毛和水母状毛。其中乳突在青冈亚属中较常见,而在壳斗科其他属中仅在石栎属 Lithocarpus 少数种类中有报道;水母状毛首次在壳斗科中发现。毛被可能遵循以下的演化规律：乳突→单毛→星状毛;星状毛依照从简单→复杂的演化途径,分化出各种形态各异和结构复杂的毛系。初步讨论了毛被以及叶表皮其他特征(如毛基细胞和表皮细胞的形态、气孔的类型和密度等)的分类和系统学意义。     

番荔枝科10种植物叶表皮结构的研究

利用光学显微镜和扫描电子显微镜,对番荔枝科10种植物的叶表皮结构进行了观察,结果如下：表皮细胞近等径多角形或不规则形,垂周壁直,弧形,浅波状,波状或深波状,瓜馥木和香港瓜馥木叶片远轴面表皮细胞平周壁形成乳突,有的植物表皮细胞内具棱晶或晶簇,气孔器全部为平列型,具2－6个副卫细胞,除鹰爪花近轴面,远轴面均具气孔器外,其它9种植物的气孔器仅分布在远轴面,有的植物还具有双气孔器或败育的气孔器类型。除圆滑番荔枝外,其它9种的表皮均具表皮毛,表皮毛有单细胞毛,单列多细胞毛,2列多细胞毛,棍棒状毛和星状毛等类型,研究结果表明,10种植物的表皮细胞形状,垂周壁的式样,平周壁是否乳突,表皮细胞内晶体的有无及其类型,近轴面是否具气孔器,表皮毛的类型等方面存在着种间差异,这些特征可以作为种间甚至属间分类和系统关系探讨的依据。     

10种卫矛科植物次生韧皮部的比较解剖

研究了卫矛科（Ｃｅｌａｓｔｒａｃｅａｅ）５ 属１０ 种植物次生韧皮部的结构。根据筛管分子、韧皮射线及厚壁组织的结构特征,将这１０ 种植物次生韧皮部分为３ 种类型。类型Ⅰ：筛管分子端壁倾斜,筛域数多,都为复筛板,韧皮射线大多为单列。卫矛属（Ｅｕｏｎｙｍ ｕｓ）的５ 种植物属于这一类型;类型Ⅱ：包括南蛇藤属（Ｃｅｌａｓｔｒｕｓ）和雷公藤属（Ｔｒｉｐｔｅｒｙｇｉｕｍ ）,其特征是筛管分子短,端壁倾斜度小,筛域数少,纤维不木质化,射线多列;类型Ⅲ：筛管分子多为单筛板,端壁接近水平,在不具功能韧皮部出现成簇的石细胞和纤维,射线单列或两列。属于此类型的有十齿花属（Ｄｉｐｅｎｔｏｄｏｎ）和核子木属（Ｐｅｒｒｏｔｔｅｔｉａ）     

魏氏拟尾柱虫腹皮层纤毛器微管胞器的形态及形态发生

应用荧光紫杉醇直接荧光标记和抗α-微管蛋白抗体免疫荧光标记显示,魏氏拟尾柱虫(Paraurostyla weissei)腹面皮层纤毛器微管胞器由口围带、波动膜、额腹横棘毛和左右缘棘毛等纤毛器微管、纤毛器基部附属微管等组成。其中口围带基部微管包括小膜托架、小膜附属微管;额腹横棘毛和左右缘棘毛基部附属微管包括前纵微管束、后纵微管束和横微管束,它们由各自的纤毛器基部向皮层细胞质不同方向发射,形成腹皮层表面下微管网。结果表明,魏氏拟尾柱虫的纤毛器骨架、纤毛器附属结构也是一类以微管蛋白为基本成分的微管胞器,其中缘棘毛基部附属微管具有不同于其他纤毛虫(例如棘尾虫)中所观察到的同种微管胞器的建构特征。形态发生中,前仔虫口围带在老结构位置形成,其结构建成与部分老口围带的更新有关;老缘棘毛的结构物质对新的左、右缘棘毛的发生可能具有定位作用及物质贡献,但此后新的左、右缘棘毛列分别在老缘棘毛的右侧形成,而并非是在老缘棘毛位置分化的。在有些细胞中,新的左缘棘毛左侧另有一列棘毛,这可能是形态发生中老的左缘棘毛退化不完全产生的。     

基于形态和叶表皮微形态特征试论国产赖草属属下类群间的系统关系

通过对先前学者报道的国产赖草属24个物种、72个代表居群形态和叶表皮微形态性状特征的观测和研究,结果发现:(1)原国产赖草属物种在旗叶长宽、叶片被毛状况、花序直曲和长宽、穗轴每节小穗数、每小穗小花数、外稃和内稃长度等15个外部形态学性状上均存在不同程度的差异,可将参试物种区分为3个不同的组;大赖草、粗穗赖草、硕花赖草和柴达木赖草多穗组植物的穗状花序粗壮呈圆锥状、直立、密集,穗轴同节着生3-多枚小穗,每小穗常含3~10小花,颖线状披针形等,致使其处于该属最原始的地位;皮山赖草、若羌赖草和格尔木赖草单穗组物种的穗状花序细弱呈线状、直立、疏松,穗轴每节仅着生1枚小穗,每小穗往往含2~5小花,颖线状披针形或锥形,这些较高级的外部形态特征使得该组植物隶属于赖草属中较高级的类群;而少穗组植物的外部形态性状通常介于前两组植物的中间过渡状态,因而它的系统地位自然也应处于多穗组和单穗组植物之间。同时,依据外部形态性状的递变趋势分析显示,3组植物具有直接的派生渊源。(2)国产赖草属植物的叶表皮皆由长细胞、短细胞、气孔器细胞和刺毛所组成,表现为典型的狐茅型;它们除在长细胞类型和壁的厚薄、气孔器保卫细胞的体积和类型等性状上具有明显重叠而显示相似外,其它多数性状如长细胞的长度和壁的波曲程度、短细胞的分布式样、副卫细胞的形状及刺毛的类型等性状上具有明显差异,其可将赖草属植物鉴分为与形态学界定结果完全相同的3个组。同时,根据3组植物及组内物种叶表皮性状的演化趋势,对各组和组内物种的演化关系和系统位置分析表明,多穗组植物最原始,少穗组植物较进化,单穗组植物最高级;多穗组可能直接派生了较进化的少穗组,并在少穗组的基础上进而产生了最高级的单穗组。赖草属属下类群的这一系统关系与利用外部形态特征所获得的系统与进化关系基本一致。     

中国特有属藤山柳属(猕猴桃科)植物的叶表皮形态及其分类学意义

由于形态特征变异和地理分布区域存在重叠,中国特有属藤山柳属(猕猴桃科)的物种划分问题长期以来存在争议,曾被分为20种或修订为含1种4个亚种的单型属。该研究选取了在形态和地理分布上有代表性的29个居群的184份标本,利用光学显微镜和扫描电镜观察了叶表皮形态和微形态特征,以探讨它们的分类学意义。结果表明:(1)藤山柳属植物叶表皮毛被的形态和微形态特征有3类,即光滑-短柱状毛、刚毛-长柱状毛/长刺毛、绒毛-单列多细胞毛,且这些特征在居群间差异明显,并各具明显的地理分布格局,支持把藤山柳属分为3类,即光滑类、刚毛类和绒毛类。(2)3类藤山柳植物在个别居群表现出部分同域分布现象,在峨眉山不同海拔高度的3个居群存在垂直地带性分布特点。(3)藤山柳属植物叶表皮的其他形态特征,如不规则型表皮细胞、6类气孔器、叶表皮初级蜡质纹饰以网状隆起为主,伴随着2～4类次级纹饰,在居群间变化多样等均没有明显的分类学意义。(4)由于具有相同的叶表皮形态特征和地理分布,建议把繁花藤山柳合并到绒毛藤山柳,故支持藤山柳属是1个正处于分化进程中的单型属,包括1个种3个亚种。     

Quantitative taxonomic analyses of Actinidia (Actinidiaceae) in China based on micromorphological characters of foliar trichomes

There are various arguments on classification of the genus Actinidia Lindl., a genus with approximately 63 species, 59 of which have been found in China. The paper investigated the characteristics of foliar trichomes of 35 taxa from China under optical microscope, including size, celluar structure, distribution and density. According to their micromorphological characteristics, foliar trichomes can be classified into the following six categories: 1) single-cell hairs; 2) uniseriate hairs, including linear, bulbous, twisted, straight-walled, and bent-walled hairs; 3) multiseriate hairs, including twisted, straight-walled and gradually sharpening, straight-walled and suddenly sharpening, bent-walled and gradually sharpening, and suddenly sharpening hairs; 4) multiseriate thick hairs, including pillar hairs, gradually sharpening thick hairs, and suddenly sharpening thick hairs; 5) stellate hairs, including parenchyma-stellate and sclerenchyma-stellate (normal state and special states such as rosulate, peltate-stellate, and overlopping-stellate) hairs; and 6) dichotomous hairs. On the basis of the micromorphological characteristics of foliar trichomes in Actinidia, with Clematoclethra lasioclada as an outgroup, both the quantitative cladistic analysis and phenetic analysis were performed using Wagner method and UPGMA clustering method respectively to reconstruct the phylogeny of Actinidia in China. The phylogenetic tree generated by cladistic analysis suggests that the sect. Leiocarpae be a monophyletic group, but other three sections, i.e., sect. Maculatae, sect. Strigosae and sect. Stellatae, be non-monophyletic groups. The results obtained from the phenetic analysis reflect relationships among the taxa of Actinidia in China, especially a close relationship between A. chinensis and A. deliciosa, and a relatively remote relationship between A. callosa var. henryi and A. callosa var. discolor. In conclusion, the micromorphological characters of foliar trichomes and the methods of quantitative taxonomic analysis are of key importance tostudies on phylogenetic and phenetic relationships of Actinidia.     

Leaf epidermal features of Lithocarpus (Fagaceae) from China and their systematic significance

The leaves of 52 species of Lithocarpus in China were studied. The adaxial leaf epidermis was investigated by light microscopy. Epidermal cells of the adaxial surface were classified into three types on the basis of the outline of their anticlinal walls, i.e. sinuate, straight and curved. The abaxial leaf epidermis was investigated by light microscopy and scanning electron microscopy. The following types of trichome were observed: appressed parallel tuft, stellate, fused stellate, papillae, stipitate fasciculate, solitary unicellular, appressed laterally attached unicellular, curly thin‐walled unicellular, bulbous and thin‐walled peltate. The fused stellate, appressed laterally attached unicellular and curly thin‐walled unicellular trichomes were reported in Lithocarpus for the first time. The appressed parallel tuft trichome, which is recognized as a salient characteristic of Lithocarpus, was not found in 15 species. A cladistic analysis was performed on the basis of the leaf epidermal features. According to the leaf epidermal features and several morphological characteristics, 26 of the 52 species could be divided into seven groups. Similar groups can be found in Barnett's and Camus' systems. The trichomes of four genera in Fagaceae are listed and compared. Lithocarpus had 14 types of trichome, 11 of which were identical to types found in Quercus, more than in Castanopsis and Cyclobalanopsis. The evolutionary trends of trichomes in Fagaceae are discussed and a new point of view is raised. © 2011 The Linnean Society of London, Botanical Journal of the Linnean Society, 2012, 168 , 216–228.     

Trichome anatomy and taxonomy of Saxifraga (Saxifragaceae)

Trichomes on flower pedicels and leaves were examined in 213 species of Saxifraga L. and in the monotypic, segregate genera Zahlbrucknera Reichenb., Saxifragella Engl., Saxifragodes D. M. Moore and Saxifragopsis Small. Pedicel trichomes were found to be better taxonomic characters than foliar ones. Six main trichome types were identified: i) multiseriate, glandular; ii) uniseriate, glandular; iii) sessile, multicellular, glandular; iv) multiseriate, eglandular; v) uniseriate, eglandular; vi) unicellular, eglandular. The different types were distributed (except in certain species) on pedicels as follows. Multiseriate, glandular types occur in four series of Saxifraga sect. Micranthes (Haw.) D. Don, part of sect. Gymnopera D. Don, and in sects Ciliatae Haw., Trachyphyllum Gaudin, Xanthizoon Griseb., Ligulatae Haw., Porophyllum Gaudin, Porphyrion Tausch and Irregulares Haw. They also occur in the genus Saxifragopsis. Uniseriate, glandular types occur in five series of Saxifraga sect. Micranthes , part of sect. Gymnopera and Irregulares , and in sects. Miscopetalum (Haw.) Sternb., Cymbalaria , Griseb. Tridactylites (Haw.) Engl., Saxifraga and Stoloniferae Haw. They also occur in the genus Zahlbrucknera. Eglandular, multicellular trichomes occur occasionally in Saxifraga sects Micranthes and Gymnopera , more frequently in sects Ciliatae and Ligulatae , and are ubiquitous in sects Xanthizoon, Porophyllum and Porphyrion. Eglandular, unicellular trichomes occur only in Saxifragopsis and Saxifragodes. Sessile, glandular trichomes occur in a group of species from Saxifraga sect. Stoloniferae. Saxifragella is glabrous. This taxonomic distribution of trichome types generally supports Engler's classification of the genus, and is compatible with other evidence from palynology, embryology and seed morphology. The taxonomic disposition of the few exceptional species is discussed.     

Systematic distribution of foliar trichome types in Crotovt (Euphorbiaceae)

Variation in foliar trichomes in Croton (Euphorbiaceae) is reviewed, and the terminology clarified by redefinitions and illustrations of terms. Evolution of trichome types appears to proceed from branched (stellate or fasciculate) hairs to simple and dendritic ones. A systematic enumeration characterizes the trichome morphology in 36 of the 40 sections, with citation of the 120 species examined.     

The foliar trichomes of Hypoestes aristata (Vahl) Sol. ex Roem. & Schult var aristata (Acanthaceae) a widespread medicinal plant species in tropical sub-Saharan Africa: with comments on its possible phylogenetic significance

The micromorphology of foliar trichomes of Hypoestes aristata var. aristata was studied using stereo, light and scanning microscopy (SEM). This genus belongs to the advanced angiosperm family Acanthaceae, for which few micromorphological leaf studies exist. Results revealed both glandular and non-glandular trichomes, the latter being more abundant on leaf veins, particularly on the abaxial surface of very young leaves. With leaf maturity, the density of non-glandular trichomes decreased. Glandular trichomes were rare and of two types: long-stalked capitate and globose-like peltate trichomes. Capitate trichomes were observed only on the abaxial leaf surface, while peltate trichomes were distributed on both adaxial and abaxial leaf surfaces.     

Morphology and distribution of trichomes in Italian species ofTeucrium sect.Chamaedrys (Labiatae) — a taxonomical evaluation

Micromorphology and distribution of trichomes (both secretory and not) in different parts of plants of the five Italian species belonging toTeucrium sect.Chamaedrys have been studied. These characters neatly evidence the differences between the taxa considered; of particular interest are the observations concerning the dense coverage of peltate hairs on the abaxial face of the leaves ofT. flavum subsp.glaucum and the characters contributing to a better identification ofT. marum andT. subspinosum. The importance of the calyx indumentum as distinctive taxonomical character of the genusTeucrium is confirmed, stressing at the same time the necessity of completing the observations at micromorphological level.     

水玉簪属系统发育重建的初步研究

以白玉簪科和兰科的代表种类分别作为外类群对水玉簪属进行了初步的分支分析。虽然运用不 同外类群进行分支分析产生的结果并不完全一致,但它们均显示了属内一些稳定“支”的结构及其种间关系。分支分析还表明：水玉簪属的两个组中,Foliosa组极可能是一个单系类群,而水玉簪组则是一并系类群。如果叶绿素的丧失是一个不可逆的进化过程,水玉簪族中全菌物异养习性的获得只出现过一 次。     

Trichome complement of Turnera and Piriqueta (Turneraceae)

The indumentum of Piriqueta and Turnera is made up of nine different types of trichomes, which broadly can be divided into glandular and non-glandular. Taking into account foot shape, head size and pedicle size, five variants of glandular trichomes are recognized: microcrapitate, stipitate-capitate, sessile-capitate and setiform. The non-glandular trichomes can be simple (unicellular or pluricellular-uniseriate), stellate or porrect-stellate. The setiform glandular hairs are present in most species of Piriqueta . Simple unicellular hairs are the most widespread type, frequently being found in combination with other trichomes. Stellate trichomes show a restricted distribution in both genera. Within Piriqueta , section Africana has only simple trichomes, whereas section Piriqueta has also porrect-stellate trichomes; groups of species can be set up according to the presence and type of glandular trichomes. Within Turnera the stipitate-capitate trichomes are exclusive to series Papilliferae ; sessile-capitate trichomes are found in series Microphyllae , Annulares and in some species of Salicifoliae ; clavate trichomes are found only in series Turnera ; setiform glandular hairs are exclusive to T. collotricha , whereas the microcapitate trichomes are widely distributed.  © 2004 The Linnean Society of London, Botanical Journal of the Linnean Society , 2004, 144 , 85–97.     

Trichome micromorphology in Alcea L. and allied genera (Malvaceae) and its systematic implication

Trichomes of 26 species of Alcea (Malvaceae) were investigated using light (LM) and scanning electron microscopy (SEM). The trichomes show a great micromorphological variation, which provides data useful for species delimitation in Alcea. Two basic types of trichomes could be distinguished in Alcea and allied genera: glandular and eglandular. The glandular trichomes could in turn be subdivided into two subtypes: capitate and clavate. The eglandular trichomes could be subdivided into five subtypes: simple, fascicled, stellate, fascicled-stellate and pluri-radiate. Characters of taxonomic interest are: trichome density (glabrous to dense), number of arms per trichome, orientation relative to the epidermal surface (appressed to erect) and presence/absence of a stalk. According to the results the species of Alcea can be divided into four informal groups based on trichome types. The results further support the exclusion of the annual Althaea species from the perennial ones and their close relationship to Malva. In addition, a close relationship between perennial Althaea and basal Alcea lineages is supported. Based on the evolutionary framework provided by recent molecular phylogenetic investigations, the following trends can be observed in the Malva alliance: long and narrow-armed trichomes are primitive in relation to short and thick-armed trichomes; dense indumentum coverage is primitive in relation to moderately dense or glabrous ones; presence of simple hairs on stem (particularly on leaves) is more advanced than their absence; spreading villous-stellate and fascicled trichomes are more advanced than appressed stellate ones. Clavate trichomes, which were found exclusively in a few species of Alcea, should be considered as a derived state in relation to capitate ones, and they may provide a synapomorphy for the crown group of Alcea.     

果皮和种皮微形态特征在杜鹃花属系统学研究中的应用

采用扫描电子显微镜对国产杜鹃花属Rhododendron6个亚属的代表种和近缘类群杜香属Ledum杜香L.palustre的果皮和种皮微形态特征进行观察,对杜鹃花属植物果皮微形态特征进行了系统描述,并通过比较现存杜鹃花属植物和种子化石,新发现一些居间的种子类型。结果表明,果皮和种皮微形态特征具有一定的系统学意义。叶状苞亚属subgen.Therorhodion的叶状苞杜鹃R.redowskianum的果实有短而疏的刺毛,无气孔,种子为无翅类,扁平,外围轮廓长椭圆形。杜鹃亚属subgen.Rhododendron植物果皮为百合花杜鹃型,具有鳞片,气孔器散生于指状突起之间,与叶表皮的微形态特征一致,其种子为百合花杜鹃型,表面具宽而浅的沟,呈脑纹状,有别于无鳞类杜鹃花。常绿杜鹃亚属subgen.Hymenanthes果皮为云锦杜鹃型,其角质层表面不规则,无表皮毛,偶见气孔;种子为云锦杜鹃型。映山红亚属subgen.Tsutsusi果皮为岭南杜鹃型,密生长表皮毛,角质层致密;种子为南边杜鹃型和岭南杜鹃型。微形态特征与“常绿杜鹃亚属和(国产)映山红亚属均为内部支持率很高的单系类群”的分子系统发育研究结果一致。马银花亚属subgen.Azaleastrum的马银花组sect.Azaleastrum和长蕊杜鹃组sect.Choniastrum微形态区别明显,支持各自为独立的单系类群。羊踯躅亚属subgen.Pentanthera的羊踯躅R.molle果皮特征明显,可与其他亚属区分,但种子类型更接近常绿杜鹃亚属。本研究结合分子系统发育资料和叶表皮微形态特征讨论了一些近缘类群的系统发育关系;研究结果支持将腺萼马银花处理为独立的种。