共查询到20条相似文献,搜索用时 31 毫秒
1.
Yong-Joo Shin Song-Hee Han Do-Sung Kim Geum-Hwa Lee Wan-Hee Yoo Yong-Mo Kang Je-Yong Choi Yong Chul Lee Seoung Ju Park Seul-Ki Jeong Hyung-Tae Kim Soo-Wan Chae Hyun-Ja Jeong Hyung-Ryong Kim Han-Jung Chae 《Arthritis research & therapy》2010,12(1):1-11
Introduction
Synovial fibroblasts from rheumatoid arthritis show resistance to apoptotic stimuli, indicating they may be difficult to treat. To clearly understand these mechanisms of resistance, rheumatoid and osteoarthritis synovial fibroblasts (RASF and OASF) were exposed to endoplasmic reticulum (ER) stress such as thapsigargin, Ca2+-ATPase inhibitor.Methods
Fibroblasts were assessed microscopically for cell viability by trypan blue exclusion and for autophagic cells by LC-3II formation. Caspase-3 activity was measured as aminomethyl-coumarin (AMC) liberated from AC-DEVD-AMC. Immunoblotting was performed to compare protein expression in OASF and RASF.Results
ER stress caused cell death in OASF but not in RASF. Thapsigargin, a Ca2+-ATPase inhibitor, did not change the expression of GRP78, an ER chaperone in OASF and RASF, but induced another ER stress protein, CCAAT/enhancer binding protein (C/EBP) homologous protein (CHOP) differently, showing high levels in OASF and low levels in RASF. Thapsigargin increased the autophagy response in RASF, with autophagosome formation, beclin expression, and LC3-II conversion. Transfection with beclin siRNA inhibited autophagy and increased the susceptibility to ER stress-induced cell death. On the other hand, CHOP siRNA increased autophagy and improved cell survival, especially in RASF, indicating that CHOP is involved in regulation of autophagy and cell death, but that low expression of CHOP protects RASF from apoptosis.Conclusions
Autophagy induction and CHOP under-expression increases cell resistance against ER stress-induced cell death in fibroblasts from rheumatoid arthritis patients. 相似文献2.
Eun Ha Kang Dong Jo Kim Eun Young Lee Yun Jong Lee Eun Bong Lee Yeong Wook Song 《Arthritis research & therapy》2009,11(4):R130-8
Introduction
Heat shock protein 70 (Hsp70) is a well-known anti-apoptotic protein that blocks multiple steps in the stress-induced apoptotic pathway. Enhanced Hsp70 expression has previously been demonstrated in rheumatoid arthritis (RA) fibroblast-like synoviocytes (FLSs). The authors investigated the role of Hsp70 in the survival of RA FLSs in a sodium nitroprusside (SNP)-treated environment. 相似文献3.
Valérie Badot Christine Galant Adrien Nzeusseu Toukap Ivan Theate Anne-Lise Maudoux Benoît J Van den Eynde Patrick Durez Frédéric A Houssiau Bernard R Lauwerys 《Arthritis research & therapy》2009,11(2):R57-13
Introduction
To identify markers and mechanisms of resistance to adalimumab therapy, we studied global gene expression profiles in synovial tissue specimens obtained from severe rheumatoid arthritis (RA) patients before and after initiation of treatment. 相似文献4.
Wu J Ekman C Jönsen A Sturfelt G Bengtsson AA Gottsäter A Lindblad B Lindqvist E Saxne T Dahlbäck B 《Arthritis research & therapy》2011,13(2):R62
Introduction
Mer and Tyro3 are receptor tyrosine kinases important for the phagocytosis of apoptotic cells. Together with Axl, they constitute the TAM receptor family. These receptors can be shed from the cell membrane and their soluble extracellular regions can be found in plasma. The objective of this study was to elucidate whether the plasma levels of soluble Mer (sMer) and Tyro3 (sTyro3) were increased in systemic lupus erythematosis (SLE), rheumatoid arthritis (RA), or critical limb ischemia (CLI). 相似文献5.
Mirjafari H Farragher TM Verstappen SM Yates A Bunn D Marshall T Lunt M Symmons DP Bruce IN 《Arthritis research & therapy》2011,13(5):R159
Introduction
Cardiovascular disease (CVD) is the leading cause of death in patients with inflammatory polyarthritis (IP), especially in seropositive disease. In established rheumatoid arthritis (RA), insulin resistance (IR) is increased and associated with CVD. We investigated factors associated with IR in an inception cohort of patients with early IP. 相似文献6.
Alejandro Balsa Arancha Cabezón Gisela Orozco Tatiana Cobo Eugenia Miranda-Carus Miguel Ángel López-Nevot José Luis Vicario Emilio Martín-Mola Javier Martín Dora Pascual-Salcedo 《Arthritis research & therapy》2010,12(2):R62
Introduction
The purpose of this study was to investigate the association between HLA-DRB1 alleles with susceptibility to rheumatoid arthritis (RA) and production of antibodies against citrullinated proteins (ACPA) and rheumatoid factor (RF). 相似文献7.
Maria D Mjaavatten Till Uhlig Anne J Haugen Halvor Nygaard Göran Sidenvall Knut Helgetveit Tore K Kvien 《Arthritis research & therapy》2009,11(5):R146-8
Introduction
The current 1987 American College of Rheumatology (ACR) classification criteria for rheumatoid arthritis (RA) have proven less useful in early arthritis. The objective of this study was to identify and compare predictors of three relevant outcomes of chronic arthritis in a cohort of very early arthritis patients. 相似文献8.
Noreen Pundt Marvin A Peters Christina Wunrau Simon Strietholt Carsten Fehrmann Katja Neugebauer Christine Seyfert Frans van Valen Thomas Pap Ingmar Meinecke 《Arthritis research & therapy》2009,11(1):R16-10
Introduction
The rheumatoid arthritis (RA) synovium is characterised by the presence of an aggressive population of activated synovial fibroblasts (RASFs) that are prominently involved in the destruction of articular cartilage and bone. Accumulating evidence suggests that RASFs are relatively resistant to Fas-ligand (FasL)-induced apoptosis, but the data concerning tumour necrosis factor-related apoptosis-inducing ligand (TRAIL) have been conflicting. Here, we hypothesise that the susceptibility of RASFs to receptor-mediated apoptosis depends on the proliferation status of these cells and therefore analysed the cell cycle dependency of FasL- and TRAIL-induced programmed cell death of RASFs in vitro. 相似文献9.
Laure Delavallée Luca Semerano Eric Assier Géraldine Vogel Grégoire Vuagniaux Marion Laborie Daniel Zagury Natacha Bessis Marie-Christophe Boissier 《Arthritis research & therapy》2009,11(6):R195
Introduction
Passive blockade of tumor necrosis factor-alpha (TNF-α) has demonstrated high therapeutic efficiency in chronic inflammatory diseases, such as rheumatoid arthritis, although some concerns remain such as occurrence of resistance and high cost. These limitations prompted investigations of an alternative strategy to target TNF-α. This study sought to demonstrate a long-lasting therapeutic effect on established arthritis of an active immunotherapy to human (h) TNF-α and to evaluate the long-term consequences of an endogenous anti-TNF-α response. 相似文献10.
Liesu Meng Wenhua Zhu Congshan Jiang Xiaojing He Weikun Hou Fang Zheng Rikard Holmdahl Shemin Lu 《Arthritis research & therapy》2010,12(3):R103
Introduction
Toll-like receptors (TLRs) are involved in both innate and adaptive immune responses and are likely to play a complex role in the pathogenesis of human rheumatoid arthritis (RA) and experimental arthritis. The objective of this study was to identify the key TLR in pristane-induced arthritis (PIA), a rat model for RA, and to clarify its roles in the initiation and maintenance of arthritis. 相似文献11.
Karen A Sutherland Helena L Rogers Denise Tosh Michael J Rogers 《Arthritis research & therapy》2009,11(2):R58
Introduction
Bisphosphonates are the most widely used class of drug for inhibiting osteoclast-mediated bone loss, but their effectiveness at preventing joint destruction in rheumatoid arthritis has generally been disappointing. We examined whether the ability of bisphosphonates to induce osteoclast apoptosis and inhibit bone resorption in vitro is influenced by the cytokine receptor activator of nuclear factor-kappa B ligand (RANKL), an important mediator of inflammation-induced bone loss. 相似文献12.
Zhu W Meng L Jiang C He X Hou W Xu P Du H Holmdahl R Lu S 《Arthritis research & therapy》2011,13(3):R103-15
Introduction
Toll-like receptors (TLRs) are likely to play crucial roles in the pathogenesis of rheumatoid arthritis (RA). The aim of this study was to determine the key TLRs in synovium and explore their roles in the activation of fibroblast-like synoviocytes (FLSs) mediated by T cells in arthritis. 相似文献13.
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15.
Syversen SW Goll GL Haavardsholm EA Bøyesen P Lea T Kvien TK 《Arthritis research & therapy》2008,10(2):R28
Introduction
Prognosis in rheumatoid arthritis (RA) is difficult to assess. The aim of this study was to examine whether serum levels of a spectrum of cytokines were predictive of radiographic progression in early RA patients. 相似文献16.
Madsen RK Lundstedt T Gabrielsson J Sennbro CJ Alenius GM Moritz T Rantapää-Dahlqvist S Trygg J 《Arthritis research & therapy》2011,13(1):R19
Introduction
The aim of this study was to assess the feasibility of diagnosing early rheumatoid arthritis (RA) by measuring selected metabolic biomarkers. 相似文献17.
Ravi C Dwivedi Navjot Dhindsa Oleg V Krokhin John Cortens John A Wilkins Hani S El-Gabalawy 《Arthritis research & therapy》2009,11(2):R32-11
Introduction
Although the clinical effects of infliximab therapy in rheumatoid arthritis have been documented extensively, the biological effects of this intervention continue to be defined. We sought to examine the impact of infliximab therapy on the serum proteome of rheumatoid arthritis patients by means of a mass spectrometry-based approach. 相似文献18.
Jeffrey R Curtis Tarun Arora Pongthorn Narongroeknawin Allison Taylor Clifton O Bingham Jack Cush Kenneth G Saag Monika Safford Elizabeth Delzell 《Arthritis research & therapy》2010,12(4):R144
Introduction
Previous research suggests patients with rheumatoid arthritis (RA) may receive suboptimal care with respect to preventive tests and services. We evaluated the proportion of older Americans with RA, psoriatic arthritis (PsA), and osteoarthritis (OA) receiving these services and the specialty of the providers delivering this care. 相似文献19.
Maria D Mjaavatten Désirée van der Heijde Till Uhlig Anne J Haugen Halvor Nygaard Göran Sidenvall Knut Helgetveit Tore K Kvien 《Arthritis research & therapy》2010,12(3):R76
Introduction
We wanted to assess the importance of the levels of anti-citrullinated peptide antibody (anti-CCP) and immunoglobulin M (IgM) rheumatoid factor (RF) in predicting development of persistent arthritis from undifferentiated arthritis (UA), and to investigate whether there is an added predictive value for persistent arthritis in testing for both anti-CCP and IgM RF. 相似文献20.
Hyun-Mi Choi Yeon-Ah Lee Sang-Hoon Lee Seung-Jae Hong Dae-Hyun Hahm Sang-Yun Choi Hyung-In Yang Myung Chul Yoo Kyoung Soo Kim 《Arthritis research & therapy》2009,11(6):R161-10