沙漠绢蒿挥发油化学成分分析1

利用气相色谱—质谱联用技术对植物沙漠绢蒿挥发油化学成分进行了分析研究。从这种植物挥发油中分出52个成分,鉴定出34个成分,所鉴定的成分占挥发油色谱总峰面积73．29％。     

小黄皮叶挥发油的化学成分

利用水蒸气蒸馏法提取小黄皮叶挥发油,运用毛细管气相色谱-质谱联用法对挥发油进行了分析,分离出40个峰,鉴定了其中的37种成分,所鉴定成分占挥发油总量的99.87%,其主要化学成分为单萜及倍半萜类化合物。     

圆瓣姜花根茎挥发油的化学成分

利用水蒸汽蒸馏法提取圆瓣姜花根茎挥发油,运用毛细管气相色谱-质谱联用法对挥发油进行了分析,分离出60个峰,鉴定了其中的51种成分,所鉴定成分占挥发油总量的97.32%,其主要化学成分为单萜及倍半萜类化合物。     

腊梅花蕾挥发性化学成分分析

利用两种不同萃取条件的超临界CO2流体萃取法提取腊梅花蕾挥发油.应用GC-MS技术对两组挥发油成分进行分离和鉴定,分别分离得到两组挥发油中的39、41个色谱峰,通过NIST2005标准谱库检索和WLEY质谱库比较,共鉴定了其中45种化学成分.结果表明:腊梅花蕾挥发油主要成分为芳樟醇、邻苯二甲酸二辛酯、β-月桂烯、桧烯、橙花叔醇、苯甲醇、己酸乙酯、β-蒎烯和榄香醇;不同萃取条件下腊梅花蕾挥发油成分差异明显.     

小叶臭黄皮叶挥发油化学成分的研究

采用水蒸气蒸馏法提取小叶臭黄皮叶挥发油,运用毛细管气相色谱-质谱联用法对挥发油成分进行了分析,共分离出84个峰,鉴定了其中的66种成分,所鉴定成分占挥发油总量的94.52%.其主要化学成分为α-芹子烯(15.76%)、石竹烯(15.05%)、β-芹子烯(9.54%)、α-蒎烯(6.43%)和α-石竹烯(5.39%)等.     

鸭儿芹根、茎、叶挥发油的化学成分

采用水蒸气蒸溜法提取其根、茎、叶中的挥发油,利用GC-MS联用仪对其化学成分进行分析,以归一化法计算各个化学成分的相对含量.鸭儿芹根挥发油中共分离出11个峰,鉴定出11种化合物,占总离子峰的98.61％,主要成分为α-芹子烯、γ-芹子烯等;茎中共分离出25个峰,鉴定出25种化合物,占总离子峰的100.00％,主要成分为...     

黄皮果挥发油成分研究

采用水蒸气蒸馏法从黄皮果中提取挥发油 ,并用GC MS法采用最佳分析条件对化学成分进行鉴定 ,GC法测定各化合物在挥发油中的相对百分含量。得到 92个化合物的峰 ,鉴定了 43个成分 ,占挥发油成分的 83 15 %以上。通过对黄皮果挥发油的分析 ,为对其及黄皮植物的进一步开发利用提供了科学依据     

新疆刺山柑叶子挥发油化学成分的研究

目的:分析新疆刺山柑叶子部位挥发油成分.方法:采用水蒸气蒸馏法提取刺山柑叶子挥发油,通过气相色谱-质谱(GC-MS)联用技术进行挥发油成分分析.结果:检出50个色谱峰,鉴定出34个化合物,占总挥发油的94.93%.结论:新疆刺山柑叶子挥发油中的主要成分为硫化物,占总挥发油的80.72%;酮类(9.19%)为次要化学成分;醛类2.36%;萜烯类2.26%;烃0.32%;醇1.09%.这些数据为进一步开发利用刺山柑资源提供了可靠的依据.     

白簕叶挥发油的化学成分

用GCMS联用技术首次研究了云南西双版纳白簕叶挥发油的化学成分,并应用色谱峰面积归一化法计算各成分的相对含量。一共分离出108个峰,确认了其中的81种成分,所鉴定的组分占挥发油总量的96.50%,主要是萜烯类及其含氧衍生物等。     

板蓝根挥发性成分GC-MS分析

采用水蒸气蒸馏法从板蓝根中提取挥发油,应用GC-MS联用检测仪对提取的挥发油进行成分分析.共得到22个峰,通过NIS107质谱数据库检索,鉴定出16个化合物,占总量的85.195,其中含量最高的是十六酸,约占总量的38.52%.应用GC-MS法分离、分析板蓝根中的挥发油成分,操作快速、简便.     

CT 与X线诊断肠梗阻的临床价值探讨

目的：探讨腹部X 线与CT 扫描鉴别急性肠梗阻的准确性,为临床诊断提供参考。方法：选取2011年8 月-2013 年8 月我院 收治的66 例急性肠梗阻患者的临床资料进行回顾分析。所有患者均经手术活检或病理诊断证实为急性肠梗阻。术后患者均行腹 部X 线及CT扫描检查,对两种方法判断肠梗阻的发生、梗阻部位、类型及病因与手术病理结果进行比较,评价并分析两种检查 方法的准确率。结果：66 例肠梗阻患者中,X线检出率为89.39%,CT 检出率为95.45%。X 线诊断小肠梗阻准确率为72.10%,CT 为86.05%;X线诊断结肠梗阻准确率为69.57%,CT 为86.96%。X线诊断肿瘤准确率为69.57%,CT 为86.96%;X线诊断肠粘连 准确率为67.86%,CT 为82.14%;X 线诊断肠套叠准确率为60.00%,CT 为80.00%;X线诊断单纯性机械性肠梗阻准确率为78.72%,CT为82.98%;X线诊断绞窄性肠梗准确率为73.68%,CT 为78.95 %。CT 对肠梗阻部位、病因及类型的诊断准确率高于腹部X 线片,差异具有统计学意义(P<0.05)。结论：腹部X 线与CT 用于诊断急性肠梗阻具有较高的准确率,但CT 对于肠梗阻部位、梗阻类型及梗阻病因的诊断优于X线片。     

CT与X线诊断肠梗阻的临床价值探讨

目的：探讨腹部X线与CT扫描鉴别急性肠梗阻的准确性,为临床诊断提供参考。方法：选取2011年8月-2013年8月我院收治的66例急性肠梗阻患者的临床资料进行回顾分析。所有患者均经手术活检或病理诊断证实为急性肠梗阻。术后患者均行腹部X线及CT扫描检查,对两种方法判断肠梗阻的发生、梗阻部位、类型及病因与手术病理结果进行比较,评价并分析两种检查方法的准确率。结果：66例肠梗阻患者中,X线检出率为89．39％,CT检出率为95．45％。X线诊断小肠梗阻准确率为72．10％,CT为86．05％;X线诊断结肠梗阻准确率为69．57％,CT为86．96％。X线诊断肿瘤准确率为69．57％,CT为86．96％;X线诊断肠粘连准确率为67．86％,CT为82．14％;X线诊断肠套叠准确率为60．00％,CT为80．00％;X线诊断单纯性机械性肠梗阻准确率为78．72％,CT为82．98％;X线诊断绞窄性肠梗准确率为73．68％,CT为78．95％。CT对肠梗阻部位、病因及类型的诊断准确率高于腹部X线片,差异具有统计学意义（P〈0．05）。结论：腹部X线与CT用于诊断急性肠梗阻具有较高的准确率,但CT对于肠梗阻部位、梗阻类型及梗阻病因的诊断优于X线片。     

Regulation of the expression of the serine dehydratase gene in the kidney and liver of the rat

Serine dehydratase was induced in the kidneys of normal rats by the administration of either glucagon or dexamethasone. The increase in enzyme activity was associated with an increase in both enzyme protein and its mRNA, which were determined respectively by Western blot and RNA blot analysis. No apparent differences were observed between kidney and liver in the molecular weights of serine dehydratase proteins and the sizes of their mRNAs. Although kidney serine dehydratase was dramatically induced by either glucagon or dexamethasone, the liver enzyme was induced by glucagon but not by dexamethasone alone in the intact rat. On the other hand, liver serine dehydratase was induced in starvation, diabetes mellitus, and a high-protein diet. The kidney enzyme could not be induced under any of these conditions.     

A cytological study on the development of the different types of visual cells in the chicken (Gallus domesticus)

The formation of visual cells and their intracellular organelles was studied in the embryonic chicken (Gallus domesticus) between stage 36 and hatching. Cilia formation was observed at stage 30 and by stage 42, outer segment formation from the cilia was evident. The inner segments appeared as buddings at stage 36. By stage 37, the buddings of double cones were observed clearly and such buddings elongated by stage 42. Both the single cones and rods appeared as buddings by stage 38 and elongation of the buddings was seen by stage 42. Oil droplets initially appeared by stage 39 in accessory cones and were observed in other cones by stage 42. Glycogen bodies were demonstrated firstly in rods and accessory cones at stage 43 and their development was completed by stage 45. In essence, all the essential elements of the visual cells were fully developed by hatching.     

The Effects of pH on the Labellar Sugar Receptor of the Fleshfly

Reproducible results describing the effects of pH on the response of the labellar sugar receptor of the fleshfly, Boettcherisca peregrina, were obtained. The response to sucrose was independent over a wide range of pH (3.0 to 10.0 for sucrose stimulation), but was inhibited fairly sharply on both sides of this range. Similar results were obtained for monosaccharide stimulation. The receptor was excited on stimulation by water above pH 12.0. The effects of high pH, both inhibitory and excitatory, were affected by the presence of salts. In the presence of 0.5 molar NaCl, for example, the pH-inhibition curve was shifted toward lower pH's by about one pH unit. The effects of low pH, on the other hand, were not affected by salts. Following Dixon's theory, it was concluded that at least five ionizable groups (loosing positive charges above pH 10.5) were located at the receptor site.     

ADP-ribosylation induced changes in the conformation of the chromatin of the brain of developing rats

Conformational changes in the chromatin of the cerebral hemisphere of 3-, 14- and 30-day old developing rats were studied before and after its ADP-ribosylation using DNase I and micrococcal nuclease (MNase). The rate and extent of digestion of chromatin by DNase I are the highest at 3-day and decline progressively thereafter. The rate and extent of digestion by MNase do not change during development. ADP-ribosylation of chromosomal proteins was carried out by incubating nuclei with NAD+ for 30 min and was followed by endonuclease digestion. Both the rate and extent of digestion by DNase I and MNase were enhanced after ADP-ribosylation which was the maximum for 3-day rats.     

青海湖鸟岛地区草地植物群落特征的研究

青海湖鸟岛地区草地群落共出现种子植物 6 3种 ,隶属 4 3属 ,19科 ,以北温带成分为主 ;从湖滨到典型地带性植被 ,物种多样性指数变化可以分为两大类型 ;随着高度增加 ,物种多样性呈现倒U型变化 ,沿着水平梯度物种多样性基本上逐渐增加。可以分为禾草、豆科固氮植物、非禾本科草类和半灌木功能组 ,物种水平与功能组水平的多样性、均匀度和丰富度相关系数分别为 0 2 79,0 4 36 ,0 0 19,均没有达到显著相关 ,功能组水平的多样性指数具有与物种水平不同的性质     

Light and electron microscopy on the sporulation of the oocysts of Eimeria brunetti. I. Development of the zygote and formation of the sporoblasts

The initial stages of sporulation in oocysts of Eimeria brunetti were examined in samples sporulated at 27 degrees C for 0, 12 and 24 hours. The initial zygote was found to be roughly spherical and to contain a number of polysaccharide granules which were congregated in one region of the organism. The cytoplasm also contained some strands of rough endoplasmic reticulum together with a number of mitochondria, some Golgi bodies, and some electron translucent vacuoles. The nucleus was large, with amorphous nucleoplasm and a nucleous. The cytoplasmic mass of the zygote was limited by a single unit membrane which possessed some micropores. After initiation of the sporulation, the metabolic activity of the organism appeared to increase as evidenced by the augmentation in the cytoplasm of the amounts of rough endoplasmic reticulum, number of Golgi bodies, and the appearance of polyribosomes. However, at this stage, the presence of large spherical bodies (anlagen of the refractile bodies of the sporozoites) constituted the most obvious change in the cytoplasm of the organism. After nuclear division the daughter nuclei were situated well separated in the cytoplasm and the polysaccharide granules were evenly distributed throughout the cytoplasm of the zygote. Eventually four sporoblasts were formed by invaginations of the limiting membrane. Each sporoblast was limited by a unit membrane and contained a nucleus, and the same cytoplasmic organelles as found in the zygote. The development of the sporoblast was initially accompanied by the appearance of a second limiting membrane.     

Effect of alcoholic intoxication on the lymphatic bed of the heart]

Cardiac lymphatic bed was studied in 120 male corpses at the age of 17--55 years, normal and of those died from alcohol intoxication and from cardiac ischemia. The methods of intratissue injection of coloured masses, impregnation after V. V. Kuprianov, histological and histochemical investigations were applied. Data in figures were obtained by means of a grid for cyto-, histo- and stereometric investigations and by a scanning integrant microspectrophotometer sim-2. Statistical data were obtained by a computer "Mir". It was demonstrated that the cardiac lymphatic system responds in a definite way to alcohol intoxication. Four forms of structural state in the lymphatic bed elements were revealed, specific density of its different components was calculated. A certain connection was noted to occur between morphological changes under the effect of toxic doses of alcohol; there was also a reaction of compensatory-adaptive elements of lymphodynamics. A number of pathological changes in cardiac tissues resulting in heart insufficiency were presented. Peculiarities in poisoning phases were demonstrated morphologically.     

Effect of the producer type on the nature of antibiotic fermentation

Fermentation processes in production of bacitracin, a polypeptide antibiotic by Bacillus licheniformis, and oleandomycin, a macrolide antibiotic by Streptomyces antibioticus, were studied comparatively. It was shown that the antibiotic-producing actinomycete was characterized by a prolonged phase of growth retardation. The highest efficiency of the control actions was observed at the beginning of the fermentation. They were aimed at intensifying the substrate usage during the growth phase and activation of cell metabolism. Controlled cultivation of the Bacillus representative was based on its capacity of achieving the maximum growth rate possible under the certain conditions. Therefore, an increase in the quantity of the synthesized antibiotic was due, under such conditions, to inhibition of the culture growth by various means including lower mass exchange intensity.