根表铁氧化物胶膜对水稻吸收Zn的影响

采用营养液培养方法研究了水稻根表形成的铁氧化物胶膜对水稻吸收Ｚｎ的影响．结果表明,在有Ｆｅ２＋的嫌气环境中,由于根际氧化作用水稻根表会形成红色的铁氧化物胶膜,根表的铁氧化物胶膜影响水稻对Ｚｎ的吸收．铁膜数量较少时,由于对Ｚｎ的富集作用有限,其对水稻Ｚｎ的吸收虽有促进作用,但不明显．随着根表铁膜数量的增加,这种促进作用也相应增加,并且在铁膜数量增加到一定值时,对水稻吸收Ｚｎ的促进作用达到最大．而后,随着铁膜数量的进一步增加,铁膜反而阻碍水稻对Ｚｎ的吸收,成为水稻吸收Ｚｎ的障碍层．在此过程中,水稻的根分泌物,特别是其中的植物铁载体对覆有铁膜水稻根系吸收Ｚｎ有促进作用．这种促进作用随铁膜数量的增加而逐渐减弱．因此,根表铁氧化物胶膜对水稻吸收Ｚｎ并不总是起促进作用,其作用的方向和程度取决于铁膜的数量．     

湿地植物根表的铁锰氧化物膜

湿地植物根系具有泌氧能力 ,使其根表及根际微环境呈氧化状态。因而 ,土壤溶液中一些还原性物质被氧化 ,如 Fe2 ,Mn2 ,形成的氧化物呈红色或红棕色胶膜状包裹在根表 ,称为铁锰氧化物膜。铁锰氧化物膜及其根际微环境是湿地植物根系吸收养分和污染物的门户 ,势必会影响这些物质的吸收。主要综述了铁锰氧化物膜的形成和组成 ,以及根表形成的氧化物膜的生态效应 ,也就是氧化物胶膜对植物根系吸收外部介质中的养分及污染物质——重金属离子的影响     

根表铁锰氧化物胶膜对不同品种水稻吸镉的影响

采用土培方法,研究了不同品种水稻吸镉的差异及其与根表铁锰氧化物胶膜的关系,结果表明：不同品种水稻其根膜,根部及地上部含镉量均存在显著性差异,且镉在不同水稻植株体内运输转移能力不同,不同水稻其根表淀积的铁锰氧化物数量也存在显著性差异,根膜及地上部的含镉量与极膜的含铁量均未达到显著性相关,但与根膜的含锰量相关性显著。     

沙田湖人工湿地植物-土壤有机碳库

在人工构建的用于处理水产养殖废水的表面流和水平潜流湿地复合系统中,对湿地植物各组织、湿地表层土壤(0～10 cm)、湿地植物根际土壤的有机碳含量季节变化规律进行研究,并对湿地植物表层土壤和湿地植物对有机碳的固定作用进行了分析.结果表明:湿地植物地上部分组织固定的有机碳含量表现为在生长期迅速增加,8月较高,进入成熟期(11月)后保持稳定,8月有机碳含量与5月有显著性差异、与11月差异不显著;湿地植物地下部分组织固定的有机碳含量低于地上部分,但季节变化与地上部分相似,5月含量最小,8月达到一个较高的值,11月与8月有机碳含量差异不大.植物进入生长期后,湿地表层土壤有机碳含量显著高于湿地上无植物时期,各湿地植物根际土有机碳含量均高于表层土,但差异不显著(睡莲除外).湿地表层土壤有机碳密度介于0.96～1.67 kg·m-2,受植物种类、植物生长季节等因素的影响.根据湿地面积和表层土壤有机碳含量,估算出沙田湖人工湿地(表面流湿地部分面积为3592 m2)0～10 cm土壤有机碳总量为5.61 t,通过植物吸收每年固定的碳量为10.34 t.     

东北羊草草地锰、铜、锌含量特征的研究

 本文研究了羊草(Aneurolepidium chinense)草地割草场3种植物必需的微量元素锰、铜、锌的含量特征。结果表明,在生长季各时期,优势种羊草各器官元素含量有很大变化,总的趋势是：根>根茎>茎>叶>穗(Zn：叶>茎),各器官之间元素含量差异显著(P<0.05)。其它种类植物根中锰、铜、锌含量也显著高于地上各器官。羊草地上部锰、铜、锌积累量在生长季中的变化近似于“S”型曲线,但各元素曲线最高点出现的时间不同。寸草苔(Carex duriuscula)和针蔺(Heleocharis acicularis)地上部锰、铜、锌积累量变化与生物量变化相似,基本为双峰型曲线。三种元素在群落中的分布规律是：根>茎>叶>穗,与生物量分布规律相同。但锰、铜在地上各器宫中积累量占植物总积累量的比率低于相应的生物量比率,而锌这两者的比率则与生物量比率相近。群落中锰、铜、锌积累量与根层土壤中锰、铜、锌总量之比分别为0.10%,0.33%和0.09%。     

超积累植物东南景天根际可溶性有机质对土壤锌吸附解吸的影响

采用盆栽和模拟试验研究了锌超积累植物东南景天根际土壤可溶性有机质(DOM)对土壤锌吸附解吸的影响.结果表明: 采用矿山土壤种植锌超积累植物东南景天后,根际土壤水溶性锌和乙酸铵提取态锌含量显著降低,而非根际土壤有效锌无明显变化.与不种植东南景天的对照土壤相比,根际土壤pH值降低了0.3个单位,有机质和DOM含量分别提高13.6%和20.9%.东南景天根际DOM对土壤锌吸附解吸的影响方式和程度与土壤类型有关.与对照DOM相比,在矿山土、黄筋泥和黄松土中,添加根际DOM使锌的最大吸附量(X_m)分别降低了17.8%、21.9%和27.7%,而非根际DOM对锌的最大吸附量没有影响.在3种供试土壤中添加根际DOM促进了锌的解吸,其中以黄松土最明显.东南景天根际DOM能降低土壤锌吸附容量,减少土壤对锌的吸附,促进吸附态锌的解吸,从而提高了根际锌的生物有效性.     

典型湿地植物与湿地农田作物铁含量的季节变化特征

分析了三江平原自然湿地的优势种小叶章（Calamagrostis angustifolia）、乌拉苔草（Carex meyeriana）、毛苔草（C. lasiocarpa）和湿地农田中的大豆（Giycine max）和水稻（Oryza sativa）各器官铁含量的季节变化特征。结果表明：3种湿地植物各器官的铁含量呈金字塔构型,成熟期（9月）时位于金字塔尖的叶的铁含量约为位于塔底的下草根层（地下20～30 cm）的3.08％、4.42％和1.18％;幼龄期（5月）的铁含量垂直剖面Logistic拟合很好,R²分别达1.0000、0.9902和0.9954;整个生长季地下部分铁含量均逐渐降低,而地上部分具有种间和器官间差异。相比之下,农作物大豆和水稻的铁库主要集中在地下根系中,且随生长季的延长而逐渐富集;地上部分的铁含量远低于根系,其中地上部分又以叶的含铁量最高,随生长期的延长有所增加,平均增长率分别为5.67%和75.61％;成熟期作为种子的大豆和稻米铁含量最低,仅相当于同期根铁含量的5.50%和0.44%。不同物种间相同器官铁含量的差异随生长期不同而变化。湿地植物对土壤铁的富集能力要大于湿地农田作物。     

六种湿地植物根际氧化还原电位的日变化

在野外条件下,研究人工湿地植物根际氧化还原电位(ORP)随时间的变化及其与主要环境因子的关系。研究了美人蕉(Canna indica Linn.)、风车草(Cyperus flabelliformis Rottb.)、芦苇(Phragmites australis Trin.ex Steud.)、水鬼蕉(Hymenocallis littoralis(Jack.)Salisb.)、紫芋(Colocasia tonoimo Nakai.)和鸢尾(Iris tectorum Maxim.)6种植物在潜流人工湿地中的根际ORP及其日变化。6种湿地植物的根际ORP日变化曲线相似,均为双峰型,双峰值出现在11:00—14:00之间,最大值出现在14:00。各植物的根际ORP日变化基本在130—350 m V之间,以水鬼蕉的变幅最大,风车草和芦苇的变幅较小。不同植物的根际ORP有较大差异,风车草和紫芋的日平均值最大,显著高于鸢尾、美人蕉和水鬼蕉(P0.05);芦苇显著高于鸢尾和美人蕉(P0.05)。ORP与光照强度和气温呈正相关,尤与气温的正相关最为显著。ORP日平均值与植物生物量有显著的正相关性,尤与地下部分生物量相关性最显著。结果表明,人工湿地植物根际ORP因不同植物、一天中不同的时间有较大差异,后者与光照和气温等环境因子密切相关。     

湿地植物根表铁膜研究进展

为了适应渍水环境,许多湿地植物都具有根系泌氧、形成铁膜的能力。因铁膜具有特殊的物理或化学结构,可以通过吸附和共沉淀作用影响元素在土壤中的化学行为和生物有效性,在植物吸收营养元素和重金属中起重要作用。综述了湿地植物根表铁膜的形成、影响因素以及根表铁膜对营养元素和重金属的生态环境效应,从表征技术方面阐述了根表铁膜的作用机制。对今后的研究方向给出如下建议:(1)扩大研究领域;(2)铁膜形成的动态变化过程;(3)铁膜对植物生理形态的影响;(4)利用先进的表征技术以确定铁膜的作用机制。     

闽江河口湿地短叶茳芏氮、磷含量与积累量季节变化

对闽江河口湿地优势植物短叶茳芏（Cyperus malaccensis）N、P养分含量与积累量进行了1年的监测。结果表明：地上部分N、P浓度的器官分配模式为花>叶>茎,绿色部分大于立枯部分,冬春季节浓度>夏秋季节;N、P积累量的器官分配为茎>叶>花,地下部分N、P浓度和积累量空间变化趋势一致,即0～15 cm>15～30 cm>30～60 cm;地上N平均浓度、N、P积累量在各个季节始终高于地下部分,但地上与地下P平均浓度的高低呈波动变化,N积累总量在秋季达到最大值（42.9 g·m^-2）,P积累总量在夏季达到最大值（7.3 g·m^-2）;P浓度和积累总量极显著地低于N（P<0.01）。从N/P比值看,N为限制短叶茳芏初级生产力的一个重要营养因子。     

Iron sulfide formation on root surfaces controlled by the life cycle of wild rice (<Emphasis Type="Italic">Zizania palustris</Emphasis>)

Iron sulfide plaques have been observed on roots of wild rice (Zizania palustris) and other wetland plants grown in sulfur-impacted freshwater ecosystems, but the mechanism of their formation and ramifications for plants have not been investigated. We exposed a model annual wetland plant, Zizania palustris, to elevated sulfate concentrations (3.1 mM) and quantified the development of iron oxide and iron sulfide precipitates on root surfaces throughout the plant life cycle. During the onset of seed production, root surfaces amended with sulfate transitioned within 1 week from iron (hydr)oxide plaques to iron sulfide plaques. During the same week, Fe(III) decreased on roots of plants not amended with sulfate but FeS did not accumulate. Prior to FeS accumulation, sulfate-amended plants had taken up the same amount of N as unamended plants. After FeS accumulation, total plant nitrogen did not increase further on sulfate-amended plants, indicating a cessation in nitrogen uptake, whereas total plant N continued to increase in unamended plants. Sulfate-amended plants produced fewer and lighter seeds with less nitrogen than unamended plants. FeS precipitation on roots may be associated with elevated sulfide and inhibited nitrogen uptake before the end of the plant’s life cycle, thus affecting the populations of this annual aquatic plant. We propose a mechanism by which a physiologically-induced decline in radial oxygen loss near the end of a plant’s life cycle initiates a precipitous decline in redox potential at the root surface and in adjacent porewater, initiating accumulation of iron sulfide plaques. These plaques could be an important locus for iron sulfide accumulation in wetland sediments.     

Red mud (RM)-Induced enhancement of iron plaque formation reduces arsenic and metal accumulation in two wetland plant species

Human activities have resulted in arsenic (As) and heavy metals accumulation in paddy soils in China. Phytoremediation has been suggested as an effective and low-cost method to clean up contaminated soils. A combined soil-sand pot experiment was conducted to investigate the influence of red mud (RM) supply on iron plaque formation and As and heavy metal accumulation in two wetland plant species (Cyperus alternifolius Rottb., Echinodorus amazonicus Rataj), using As and heavy metals polluted paddy soil combined with three rates of RM application (0, 2%, 5%). The results showed that RM supply significantly decreased As and heavy metals accumulation in shoots of the two plants due to the decrease of As and heavy metal availability and the enhancement of the formation of iron plaque on the root surface and in the rhizosphere. Both wetland plants supplied with RM tended to have more Fe plaque, higher As and heavy metals on roots and in their rhizospheres, and were more tolerant of As and heavy metal toxicity. The results suggest that RM-induced enhancement of the formation of iron plaque on the root surface and in the rhizosphere of wetland plants may be significant for remediation of soils contaminated with As and heavy metals.     

Effect of iron plaque outside roots on nutrient uptake by rice (Oryza sativa L.). Zinc uptake by Fe-deficient rice

This solution culture study examined the effect of the deposition of iron plaque on zinc uptake by Fe-deficient rice plants. Different amounts of iron plaque were induced by adding Fe(OH)₃ at 0, 10, 20, 30, and 50 mg Fe/L in the nutrient solution. After 24 h of growth, the amount of iron plaque was correlated positively with the Fe(OH)₃ addition to the nutrient solution. Increasing iron plaque up to 12.1 g/kg root dry weight increased zinc concentration in shoots by 42% compared to that at 0.16 g/kg root dry weight. Increasing the amount of iron plaque further decreased zinc concentration. When the amounts of iron plaque reached 24.9 g/kg root dry weight, zinc concentration in shoots was lower than that in shoots without iron plaque, implying that the plaque became a barrier for zinc uptake. While rice plants were pre-cultured in –Fe and +Fe nutrient solution in order to produce the Fe-deficient and Fe-sufficient plants and then Fe(OH)₃ was added at 20, 30, and 50 mg Fe/L in nutrient solution, zinc concentrations in shoots of Fe-deficient plants were 54, 48, and 43 mg/kg, respectively, in contrast to 32, 35, and 40 mg/kg zinc in shoots of Fe-sufficient rice plants. Furthermore, Fe(OH)₃ addition at 20 mg Fe/L and increasing zinc concentration from 0.065 to 0.65 mg Zn/L in nutrient solution increased zinc uptake more in Fe-deficient plants than in Fe-sufficient plant. The results suggested that root exudates of Fe-deficient plants, especially phytosiderophores, could enhance zinc uptake by rice plants with iron plaque up to a particular amount of Fe.     

8种湿地植物不同苗龄植株的表型特征及相关性分析

对水葱(Scirpus validus Vahl)、香蒲(Typha orientalis Presl)、小香蒲(T.minima Funk.)、再力花(Thalia dealbata Fraser ex Roscoe)、黄菖蒲(Iris pseudacorus Linn.)、灯芯草(Juncus effusus Linn.)、梭鱼草(Pontederia cordata Linn.)和菖蒲(Acorus calamus Linn.)8种多年生湿地植物1年生和3年生植株的地上和地下部分干质量、株高(包括花序高和叶层高)、根长、根数和根粗进行了测定,并对各表型特征指标间以及苗龄与各表型特征指标间的相关性进行了分析。结果显示:8种湿地植物3年生植株的地上和地下部分干质量普遍高于1年生植株;从生长量分配情况看,除小香蒲外,其余种类3年生植株地下部分干质量所占比例均高于1年生植株。多数种类1年生和3年生植株的株高差异较小。从根系特征看,根数小于100、根粗1.0~2.0 cm的植株以1年生为主,而根数大于100、根粗1.0 cm以下和2.0~3.5 cm的植株以3年生为主;根长15~25 cm的植株以1年生所占比例较高(62.5%),而根长25~35 cm的植株1年生和3年生所占比例相等。相关性分析结果表明:1年生植株的地上部分干质量与株高、根长与根粗呈显著正相关(P<0.05),3年生植株的地下部分干质量与根粗也呈显著正相关;但不同苗龄植株的其他表型特征指标间的相关性均不显著。苗龄与植株地下部分干质量呈极显著正相关(P<0.01),但与地上部分干质量、株高、根长、根数和根粗的相关性不显著。总体而言,8种湿地植物3年生植株的表型特征优于1年生植株,更适用于污染水体及退化湿地生态系统的修复。     

The impact of iron plaque on La and Nd uptake and translocation in rice (Oryza sativa L.)

The effect of root surface iron plaque formation on the uptake, transfer and accumulation of La and Nd in the rice root system was evaluated by using solution cultures. The results showed that La and Nd pollution stress inhibit formation of rice root surface iron plaques. The amount of La and Nd absorbed by the rice root surface iron plaque rose with the increase of La and Nd solution concentrations. Iron plaque formation on the rice root surface significantly decreases the La and Nd concentrations in rice roots and shoots. At growth solution La concentrations of 0.1, 0.5, and 1.0 mmol.L^? 1, concentrations of La in rice roots with induced iron plaques decreased by 17.1%, 37.4%, and 31.2%, respectively, and concentrations of La in rice shoots decreased by 43.9%, 60.6%, and 27.0%, respectively, when compared to plants with non-induced iron plaques. Also, with Nd solution concentrations of 0.1, 0.5, and 1.0 mmol.L^? 1, the Nd concentrations in rice roots and shoots of plants with induced iron plaques decreased by 21.0–31.7% and 22.7–47.5%, respectively when compared to plants with non-induced iron plaques. Iron plaque formation on the rice root surface affects the accumulation and transfer of La and Nd in rice roots. Accumulation of La and Nd was greater in rice roots than in rice shoots regardless of whether the plants had induced or non-induced iron plaques. Transfer coefficients of iron plague on rice root surface and root system under La treatments were both higher than those under Nd treatment. For rice roots and iron plaques on the root surface, the enrichment coefficient in the La treatment group was less than that in the Nd treatment group, while for rice shoots, the enrichment coefficient in the La treatment group was greater than that in the Nd treatment group. Clearly, the mechanisms governing the effect of iron plaque on La and Nd uptake and transfer in the rice root system are rather complicated.     

Root iron plaque formation and characteristics under N2 flushing and its effects on translocation of Zn and Cd in paddy rice seedlings (Oryza sativa)

Background and Aims

Anoxic conditions are seldom considered in root iron plaque induction of wetland plants in hydroponic experiments, but such conditions are essential for root iron plaque formation in the field. Although ferrous ion availability and root radial oxygen loss capacity are generally taken into account, neglect of anoxic conditions in root iron plaque formation might lead to an under- or over-estimate of their functional effects, such as blocking toxic metal uptake. This study hypothesized that anoxic conditions would influence root iron plaque formation characteristics and translocation of Zn and Cd by rice seedlings.

Methods

A hydroponic culture was used to grow rice seedlings and a non-disruptive approach for blocking air exchange between the atmosphere and the induction solution matrix was applied for root iron plaque formation, namely flushing the headspace of the induction solution with N₂ during root iron plaque induction. Zn and Cd were spiked into the solution after root iron plaque formation, and translocation of both metals was determined.

Key Results

Blocking air exchange between the atmosphere and the nutrient solution by N₂ flushing increased root plaque Fe content by between 11 and 77 % (average 31 %). The N₂ flushing treatment generated root iron plaques with a smoother surface than the non-N₂ flushing treatment, as observed by scanning electron microscopy, but Fe oxyhydroxides coating the rice seedling roots were amorphous. The root iron plaques sequestrated Zn and Cd and the N₂ flushing enhanced this effect by approx. 17 % for Zn and 71 % for Cd, calculated by both single and combined additions of Zn and Cd.

Conclusions

Blocking of oxygen intrusion into the nutrient solution via N₂ flushing enhanced root iron plaque formation and increased Cd and Zn sequestration in the iron plaques of rice seedlings. This study suggests that hydroponic studies that do not consider redox potential in the induction matrices might lead to an under-estimate of metal sequestration by root iron plaques of wetland plants.     

Iron plaque decreases cadmium accumulation in Oryza sativa L. and serves as a source of iron

• Cadmium (Cd) contamination occurs in paddy soils; hence it is necessary to reduce Cd content of rice. Application and mode of action of ferrous sulphate in minimizing Cd in rice was monitored in the present study.
• Pot culture with Indian rice variety Swarna (MTU 7029) was maintained in Cd‐spiked soil containing ferrous sulphates, which is expected to reduce Cd accumulation in rice. Responses in rhizosphere pH, root surface, metal accumulation in plant and molecular physiological processes were monitored.
• Iron plaque was induced on root surfaces after FeSO4 application and the amount of Fe in plaque reduced with increases in Cd in the soil. Rhizosphere pH decreased during plaque formation and became more acidic due to secretion of organic acids from the roots under Cd treatment. Moreover, iron chelate reductase activity increased with Cd treatment, but in the absence of Cd, activity of this enzyme increased in plaque‐induced plants. Cd treatment caused expression of OsYSL18, whereas OsYSL15 was expressed only in roots without iron plaque. Fe content of plants increased during plaque formation, which protected plants from Cd‐induced Fe deficiency and metal toxicity. This was corroborated with increased biomass, chlorophyll content and quantum efficiency of photo‐synthesis among plaque‐induced plants.
• We conclude that ferrous sulphate‐induced iron plaque prevents Cd accumulation and Fe deficiency in rice. Iron released from plaque via organic acid mediated dissolution during Cd stress.

     

Iron plaque enhances phosphorus uptake by rice (Oryza sativa) growing under varying phosphorus and iron concentrations

Both solution culture and pot experiments were performed to investigate (a) the effects of external Fe (II) concentrations and forms on the formation of iron plaque on the roots of rice (Oryza sativa) and subsequent P adsorption on iron plaque and shoot P concentrations and (b) the effects of soil moisture regimes on the formation of iron plaque and P adsorption on root surfaces and P accumulation in shoots. The results showed that iron plaque was significantly increased with increasing Fe²⁺ concentrations in the solution culture. The amounts of P adsorbed on the iron plaque were increased significantly with external Fe²⁺ concentrations. Although shoot P concentration was not significantly affected by Fe²⁺ treatment after incubation for 2 days, it was significantly increased in the Fe‐treated plants compared with Fe‐deprived ones after incubation for 4 days. Soil culture experiment showed that the formation of iron plaque on root surfaces was promoted by exogenous iron, with greater amount of iron plaque being formed by addition of ferric hydroxide than of ferric oxide. Phosphorus adsorption on iron plaque also increased with the addition of iron oxides, and increasing soil P increased the amounts of P associated with the iron plaque and shoot P concentration. The amounts of iron plaque were almost sixfold higher under flooding condition than under field capacity condition. Plants pretreated under flooding condition generally had higher shoot P concentrations when they were transplanted to solutions with varying P levels, and this was most pronounced in the treatment with highest solution P concentration. The results suggest that iron plaque acts as a nutrient reservoir for phosphorus in the rhizosphere and helps enhance P acquisition by rice.