CO2 supplementation eliminates sugar-rich media requirement for plant propagation using a simple inexpensive temporary immersion photobioreactor

Plant Cell, Tissue and Organ Culture (PCTOC) - In vitro plant propagation systems such as temporary immersion bioreactors (TIBs) are valuable tools that enable production of disease-free plants...     

A novel regeneration system through micrografting for Argania spinosa (L.) Skeels,and confirmation of successful rootstock-scion union by histological analysis

Plant Cell, Tissue and Organ Culture (PCTOC) - European mistletoe (Viscum album) is a medicinal plant with significant anticancer properties. In vitro callus production provides an essential...     

Correction to: Efficient in vitro plant regeneration from leaf-derived callus and genetic fidelity assessment of an endemic medicinal plant Ranunculus wallichianus Wight & Arnn by using RAPD and ISSR markers

Plant Cell, Tissue and Organ Culture (PCTOC) - In this study, we developed a repeatable in vitro micropropagation protocol for the medicinal plant Asparagus cochinchinensis, based on indirect...     

E. coli cultures expressing a synthetic sequence of ptz gene (stz) promoted in vitro direct organogenesis in Nicotiana tabacum L.

Plant Cell, Tissue and Organ Culture (PCTOC) - In vitro plant organogenesis requires plant regulator cytokinins to be exogenously supplied to the culture media. Cytokinins are either obtained from...     

Plant regeneration through direct and indirect organogenesis,phyto-molecular profiles,antioxidant properties and swertiamarin production in elicitated cell suspension cultures of Swertia minor (Griseb.) Knobl

Plant Cell, Tissue and Organ Culture (PCTOC) - The present study reports an optimized protocol for high frequency in vitro plant propagation through direct and indirect organogenesis, phytochemical...     

The influential role of polyamines on the in vitro regeneration of pea (Pisum sativum L.) and genetic fidelity assessment by SCoT and RAPD markers

Plant Cell, Tissue and Organ Culture (PCTOC) - A proficient and reliable in vitro plant regeneration protocol was established for pea by utilizing cotyledonary node as an explants, which excised...     

An efficient protocol for in vitro propagation of Trichilia pallida Swartz (Meliaceae): a potential tree for natural insecticide production

Plant Cell, Tissue and Organ Culture (PCTOC) - In vitro and ex vitro germination was successfully achieved for seedling production from monoembryonic and polyembryonic seeds. The seedlings were...     

Efficient slow-growth conservation and assessment of clonal fidelity of Ullucus tuberosus Caldas microshoots

Plant Cell, Tissue and Organ Culture (PCTOC) - Slow-growth is a biotechnological tool for medium-term conservation of plant germplasm under in vitro conditions. In the present study, we assessed...     

Explant disinfection with chlorine dioxide promotes adventitious shoot organogenesis in Crassula portulacea via the regulation of reactive oxygen species and related gene expression

Plant Cell, Tissue and Organ Culture (PCTOC) - Abiotic oxidative stress may activate plant organ development and growth. However, this effect is rarely reported for in vitro tissue culture. We...     

Application of artificial neural network and machine learning algorithms for modeling the in vitro regeneration of chickpea (Cicer arietinum L.)

Plant Cell, Tissue and Organ Culture (PCTOC) - In vitro whole plant regeneration protocol of desi chickpea was established followed by generating the model prediction using different machine...     

Current status and future perspectives of somatic embryogenesis in Lilium

Plant Cell, Tissue and Organ Culture (PCTOC) - An efficient in vitro plant regeneration method via somatic embryogenesis has been established in Ormosia henryi Prain, which is an important precious...     

Elicitation of phenylpropanoids in maqui (Aristotelia chilensis [Mol.] Stuntz) plants micropropagated in photomixotrophic temporary immersion bioreactors (TIBs)

Plant Cell, Tissue and Organ Culture (PCTOC) - A biotechnological system for the production of plants biomass and phenylpropanoids of maqui was developed in photomixotrophic TIBs. The in vitro...     

Transcriptional profiling of the AFL subfamily of B3-type transcription factors during the in vitro induction of somatic embryogenesis in the model legume Medicago truncatula

Plant Cell, Tissue and Organ Culture (PCTOC) - In many legume species, somatic embryogenesis is a limiting step of in vitro regeneration, with profound implications to genetic engineering and plant...     

High throughput in vitro seed germination screen identified new ABA responsive RING-type ubiquitin E3 ligases in Arabidopsis thaliana

Plant Cell, Tissue and Organ Culture (PCTOC) - Seed quality is an important factor for seedling vigour as well as adult plant resilience. The key quality attributes are related to physical...     

Schisandra henryi C. B. Clarke in vitro cultures: a promising tool for the production of lignans and phenolic compounds

Plant Cell, Tissue and Organ Culture (PCTOC) - We initiated and optimized in vitro culture conditions of the endemic Chinese plant species—Schisandra henryi C. B. Clarke. Different types of...     

SmMYB98b positive regulation to tanshinones in Salvia miltiorrhiza Bunge hairy roots

Plant Cell, Tissue and Organ Culture (PCTOC) - Tanshinones are major secondary metabolites in Salvia miltiorrhiza Bunge, the traditional Chinese medicinal plant Danshen. Increasing the production...     

Efficient plant regeneration system for New Guinea Impatiens (Impatiens hawkeri W. Bull) CV. ‘Violet’ and ‘Scarlet Bronze Leaf’

Plant Cell, Tissue and Organ Culture (PCTOC) - New Guinea Impatiens (Impatiens hawkeri W. Bull) is an eye-popping landscaping plant of bright and colorful blooms. A highly efficient in vitro plant...     

Cytotoxic,clonal fidelity,and antioxidant evaluation of in vitro propagated Vitex negundo var. cannabifolia

Plant Cell, Tissue and Organ Culture (PCTOC) - Vitex negundo L. var. cannabifolia (Sieb. et Zucc.) Hand.-Mazz. is an important medicinal plant rich in bioactive compounds. In vitro tissue culture...     

Workability and productivity of robotic plug transplanting workcell

Summary Transplanting is a necessary operation in transplant production systems. Transplanting operation is labor-intensive and automation can reduce labor costs. Plugs are actively growing young transplants with two well-defined morphologic parts: the stem-leaf portion and the root-growth medium portion. They may be grown in regularly situated cells on traylike containers. This regularity makes plugs suitable for automated transplanting operations. It is, therefore, beneficial for in vitro plant propagation systems to include plugs as intermediate products before they are delivered to the greenhouses. Flexible automation and robotics technologies have been applied to develop a robotic workcell for transplanting plugs from plug trays to growing flats. Main components of the workcell include a robot, an end-effector, and two conveyer belts for transporting trays and flats. The end-effector for extracting, holding, and planting plugs is a “sliding-needles-with-sensor” gripper. The sensor signals the robot to complete a transplanting cycle only when a plug is properly held by the gripper. Systems analysis and computer simulation were conducted to study factors affecting workability and productivity of various workcell designs. These factors included: dimensions and kinematics of the robot and its peripheral equipment, layout and materials flow, fullness of plug trays, and successful extraction rate of plugs. The analysis also indicated that machine vision systems could add valuable capabilities to the workcell, such as robot guidance and plug quality evaluation. Engineering economic analysis was performed to investigate the interaction of workcell technical feasibility and economic viability. Presented in the Session-in-Depth Robotics in Tissue Culture at the 1991 World Congress on Cell and Tissue Culture, Anaheim, California, June 16–20, 1991.     

Optimisation of PD-FcY veterinary antigen secretion from Nicotiana benthamiana hairy roots and purification from the culture medium

Plant Cell, Tissue and Organ Culture (PCTOC) - Hairy roots (HR) are a promising plant tissue-based platform for the production of biopharmaceuticals as the secretion of recombinant proteins in the...