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1.
A Gram-staining positive, endospore-forming, motile and rod-shaped bacterial strain, BR-29 T, was isolated from soil from west coast of the Korean peninsula, and its taxonomic position was investigated by a polyphasic
study. Strain BR-29 T grew optimally at around pH 7.5, at 30°C and in the presence of 0.5% (w/v) NaCl. Phylogenetic analyses based on 16S rRNA
gene sequences showed that strain BR-29 T fell into a clade comprising the type strains of Cohnella species, with which it exhibited 16S rRNA gene sequence similarity values of 92.8–96.4%. Strain BR-29 T contained a cell wall peptidoglycan based on meso-diaminopimelic acid and MK-7 as the predominant menaquinone. The major fatty acids were anteiso-C 15:0, C 16:0 and iso-C 16:0. The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, lysylphosphatidylglycerol and two unidentified
phospholipids; a minor amount of phosphatidylglycerol was present. The DNA G+C content was 54.9 mol%. Strain BR-29 T could be differentiated from phylogenetically related Cohnella species by differences in phenotypic characteristics. On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain
BR-29 T represents a novel species of the genus Cohnella, for which the name Cohnella
boryungensis sp. nov., is proposed. The type strain is BR-29 T (= KCTC 13735 T = CCUG 59598 T). 相似文献
2.
A Gram-positive, coccoid to rod-shaped, non-spore-forming bacterium, designated Gsoil 958 T, was isolated from soil of a ginseng field located in Pocheon province in South Korea. This bacterium was characterized in order to determine its taxonomic position by using a polyphasic approach. Strain Gsoil 958 T was observed to grow well at 25–30 °C and at pH 7.0 on R2A and nutrient agar without NaCl supplementation. Strain Gsoil 958 T was determined to have β-glucosidase activity and the ability to transform ginsenoside Rb 1 (one of the dominant active components of ginseng) to F 2 via gypenoside XVII and Rd. On the basis of 16S rRNA gene sequence similarity, strain Gsoil 958 T was shown to belong to the family Nocardioidaceae and related most closely to Nocardioides koreensis MSL-09 T (97.6 % 16S rRNA gene sequence similarity), Nocardioides aquiterrae GW-9 T (97.0 %), and Nocardioides sediminis MSL-01 T (97.0 %). The sequence similarities with other validly named species within the genus Nocardioides were less than 96.8 %. Strain Gsoil 958 T was characterized chemotaxonomically as having LL-2,6-diaminopimelic acid in the cell-wall peptidoglycan, MK-8(H 4) as the predominant menaquinone, and iso-C 16:0, iso-C 16:1 H, iso-C 14:0, iso-C 15:0 were identified as the major fatty acids. The G + C content of genomic DNA was determined to be 70.8 mol %. The chemotaxonomic properties and phenotypic characteristics supported the affiliation of strain Gsoil 958 T to the genus Nocardioides. The results of both physiological and biochemical tests allowed for differentiation of strain Gsoil 958 T from the recognized Nocardioides species. Therefore, strain Gsoil 958 T is considered to represent a novel species of the genus Nocardioides, for which the name Nocardioides panaciterrulae sp. nov. is proposed, with the type strain Gsoil 958 T (KACC 14271 T = KCTC 19471 T = DSM 21350 T). 相似文献
3.
A novel bacterial strain, designated T-Y1 T, capable of degrading a variety of polysaccharides was isolated from seawater of an oyster farm in the South Sea, Korea. It was found to be aerobic, Gram-negative, non-flagellated, non-gliding and rod-shaped. Strain T-Y1 T grew optimally at 25 °C, at pH 7.0–7.5 and in the presence of 2 % (w/v) NaCl. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain T-Y1 T belonged to the genus Winogradskyella. Strain T-Y1 T exhibited 16S rRNA gene sequence similarity values of 95.0–96.8 % to the type strains of recognized Winogradskyella species and less than 94.5 % to other validly named species. The chemotaxonomic data concurred with the phylogenetic inference. Strain T-Y1 T contained MK-6 as the predominant menaquinone and anteiso-C 15:0, iso-C 15:0, iso-C 15:1 G and iso-C 16:0 3-OH as the major fatty acids. The major polar lipids of strain T-Y1 T were phosphatidylethanolamine and two unidentified lipids. The DNA G+C content was 36.2 mol%. Differential phenotypic properties, together with its phylogenetic distinctiveness, enabled strain T-Y1 T to be differentiated from the recognized Winogradskyella species. On the basis of the data presented here, strain T-Y1 T is considered to represent a novel species of the genus Winogradskyella, for which the name Winogradskyella multivorans sp. nov. is proposed. The type strain is T-Y1 T (=KCTC 23891 T = CCUG 62216 T). 相似文献
4.
A taxonomic study was carried out on Gsoil 142 T, a bacterial strain isolated from the soil collected in a ginseng field in Pocheon province, South Korea. Comparative 16S
rRNA gene sequence studies showed a clear affiliation of this bacterium to the Gammaproteobacteria, and it was most closely related to Hydrocarboniphaga effusa ATCC BAA 332 T (94.4%, 16S rRNA gene sequence similarity), Nevskia ramosa DSM 11499 T (94.1%) and Alkanibacter difficilis MN154.3 T (92.0%). Strain Gsoil 142 T was a Gram-negative, strictly aerobic, motile, and rod-shaped bacterium. The G+C content of the genomic DNA was 69.9% and
predominant ubiquinone was Q-8. Major fatty acids were summed feature 8 (C 18:1
ω7 c and/or ω6 c, 36.3%), summed feature 3 (iso-C 15:0 2-OH and/or C 16:1
ω7 c, 20.6%) and C 16:0 (17.4%). The major polar lipids detected in strain Gsoil 142 T were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, and an unknown glycolipid. On the basis of polyphasic
evidence, it is proposed that strain Gsoil 142 T should be placed in a novel genus and species, for which the name Panacagrimonas perspica gen. nov., sp. nov. is proposed. The type strain is Gsoil 142 T (= KCTC 12982 T = LMG 23239 T). 相似文献
5.
A strictly aerobic, Gram-negative, reddish-orange pigmented, non-motile and rod-shaped bacterium, designated AK17-053 T was isolated from a marine crustacean ( Squillidae) living on tidal flats on the coast of the Ariake Sea, Nagasaki, Japan. Phylogenetic analysis based on the 16S rRNA gene
sequence revealed that the novel isolate could be affiliated with the family Saprospiraceae of the phylum Bacteroidetes and that it showed highest sequence similarity (84%) with Lewinella marina MKG-38 T. The strain could be differentiated phenotypically from recognized members of the family Saprospiraceae. The G+C content of DNA was 55.3 mol%, MK-7 was the major menaquinone and iso-C 15:0 and C 16:1ω7c were the major fatty acids. On the basis of polyphasic taxonomic studies, it was concluded that strain AK17-053 T represents a new genus of the family Saprospiraceae. We propose the name Rubidimonas crustatorum gen. nov., sp. nov. for this strain; its type strain is AK17-053 T (= MBIC08356 T = NBRC 107717 T). 相似文献
6.
A Gram-negative, aerobic, non-flagellated, non-gliding and rod-shaped bacterial strain, designated M-M16 T, was isolated from seashore sand around a seaweed farm on the South Sea, South Korea, and its taxonomic position was investigated by using a polyphasic study. Strain M-M16 T grew optimally at 30 °C, at pH 7.0–8.0 and in the presence of 2 % (w/v) NaCl. Strain M-M16 T exhibited the highest 16S rRNA gene sequence similarity values to the type strains of Gaetbulibacter lutimaris (96.5 %) and Flaviramulus basaltis (95.8 %). Neighbour-joining and maximum-parsimony phylogenetic trees based on 16S rRNA gene sequences revealed that strain M-M16 T clustered with the type strains of Gaetbulibacter species and F. basaltis. Strain M-M16 T contained MK-6 as the predominant menaquinone and iso-C 15:1 G, iso-C 15:0 and iso-C 17:0 3-OH as the major fatty acids. The major polar lipids detected in strain M-M16 T were phosphatidylethanolamine and one unidentified lipid. The DNA G+C content of strain M-M16 T was 37.4 mol%. The phylogenetic and chemotaxonomic data and other phenotypic properties revealed that strain M-M16 T represents a novel genus and species within the family Flavobacteriaceae, for which the name Sabulilitoribacter multivorans gen. nov., sp. nov. is proposed. The type strain of S. multivorans is M-M16 T (= KCTC 32326 T = CCUG 63831 T). 相似文献
7.
A Gram-negative, coccoid shaped bacterium isolated from the outer surface of the medicinal leech Hirudo medicinalis was characterized. The 16S rRNA gene sequence comparison revealed that the bacterium was closely related to species of the genus Luteolibacter. Luteolibacter pohnpeiensis was the most closely related species (94.6 % sequence similarity), followed by Luteolibacter luojiensis (93.4 %) and Luteolibacter algae (93.3 %). Chemotaxonomic data (major ubiquinone: MK-9; major polar lipids: phosphatidylethanolamine and phosphatidylglycerol; and major fatty acids: iso-C 14:0, C 16:0, iso-C 16:1, and anteiso-C 15:0) supported the affiliation of the isolate to the genus Luteolibacter. DNA–DNA hybridizations with the type strain of L. pohnpeiensis was 31 % (reciprocal value 30 %). A phenotypic differentiation of strain E100 T from L. pohnpeiensis and the other Luteolibacter species was possible by several physiological tests. We conclude Strain E100 T represents a novel species, for which we propose the name Luteolibacter cuticulihirudinis sp. nov. with the type strain E100 T (=CCM 8400 T = LMG 26924 T). 相似文献
8.
A Gram-negative, non-flagellated, non-gliding and rod-shaped, coccoid or filamentous bacterial strain, designated YCS-16 T, was isolated from coastal seawater from a seaweed farm on the South Sea, South Korea, and its taxonomic position was investigated by using a polyphasic study. Strain YCS-16 T was observed to grow optimally at 30 °C, at pH 7.0–7.5 and in the presence of 2 % (w/v) NaCl. Strain YCS-16 T exhibited the highest 16S rRNA gene sequence similarity values to the type strains of Bizionia echini (96.1 %), Formosa spongicola (95.8 %), Bizionia algoritergicola (95.5 %) and Psychroserpens mesophilus (95.4 %). Neighbour-joining and maximum-parsimony phylogenetic trees based on 16S rRNA gene sequences revealed that strain YCS-16 T joined the cluster comprising the type strains of Psychroserpens species. Strain YCS-16 T was found to contain MK-6 as the predominant menaquinone and iso-C 17:0 3-OH, iso-C 15:1 G and iso-C 15:0 as the major fatty acids. The major polar lipids detected in strain YCS-16 T were phosphatidylethanolamine and one unidentified lipid. The DNA G+C content of strain YCS-16 T was determined to be 35.7 mol%. The phylogenetic analysis, chemotaxonomic data and other phenotypic properties revealed that strain YCS-16 T constitutes a new genus and species within the family Flavobacteriaceae, for which the name Geojedonia litorea gen. nov., sp. nov. is proposed. The type strain of Geojedonia litorea is YCS-16 T (=KCTC 32260 T = CCUG 63682 T). 相似文献
9.
A Gram-positive, rod-shaped, non-spore-forming bacterium (Gsoil 485 T) was isolated from the soil of a ginseng field located in Pocheon province in South Korea. This bacterium was characterized
in order to determine its taxonomic position by using the polyphasic approach. On the basis of 16S rRNA gene sequence similarity,
strain Gsoil 485 T was shown to belong to the family Nocardioidaceae and related to Nocardioides koreensis (96.8% 16S rRNA gene sequence similarity), Nocardioides basaltis (96.7%), Nocardioides salarius (96.7%), and Nocardioides sediminis (96.5%). The sequence similarity with other species that had validly published names within the genus Nocardioides was less than 96.4%. Strain Gsoil 485 T was characterized chemotaxonomically as having LL-2,6-diaminopimelic acid in a cell-wall peptidoglycan, MK-8(H 4) as the predominant menaquinone, and iso-C 16:0, C 18:1
ω9 c as the major fatty acids. The G+C content of genomic DNA was 71.6 mol%. The chemotaxonomic properties and phenotypic characteristics
supported the affiliation of strain Gsoil 485 T to the genus Nocardioides. The results of both physiological and biochemical tests allowed for genotypic differentiation of strain Gsoil 485 T from the recognized Nocardioides species. Therefore, strain Gsoil 485 T is considered to represent the novel species, for which the name Nocardioides
ginsengisegetis sp. nov. is proposed, with the type strain Gsoil 485 T (KACC 14269 T =KCTC 19469 T =DSM 21349 T). 相似文献
10.
A bacterial strain designated antisso-27 T, previously isolated from saltpan in Taiwan while screening for bacteria for algicidal activity, was characterized using
the polyphasic taxonomic approach. Strain antisso-27 T was Gram-negative, aerobic, brownish yellow colored, rod-shaped, non-flagellated and non-gliding. Phylogenetic analyses based
on 16S rRNA gene sequences showed that strain antisso-27 T belonged to the genus Aquimarina within the family Flavobacteriaceae with relatively low sequence similarities of 94.0–96.6% to other valid Aquimarina spp. It contained iso-C 17:0 3-OH, iso-C 15:0, iso-C 16:0, iso-C 15:1 and iso-C 15:0 3-OH as the main fatty acids and contained a menaquinone with six isoprene units (MK-6) as the major isoprenoid quinone.
Major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, an uncharacterized aminolipid and five uncharacterized
phospholipids. Strain antisso-27 T employed direct mode of algicidal lysis to Chlorella vulgaris strain 211-31; nevertheless, it released an algicidal substance against M. aeruginosa strain MTY01. This is the first study that the Aquimarina species possesses both direct and indirect algicidal activities. On the basis of the phylogenetic and phenotypic data, strain
antisso-27 T should be classified as representing a novel species, for which the name A. salinaria sp. nov. is proposed. The type strain is A. salinaria antisso-27 T (= BCRC 80080 T = LMG 25375 T). 相似文献
11.
Strain S22 T, a novel cellulolytic bacterium was isolated from the rhizosphere of pine trees. This isolate was Gram-reaction positive,
motile and rods, and formed terminal or subterminal ellipsoidal spores. S22 T represented positive activity for catalase, oxidase, esterase (C4), esterase lipase (C8), β-galactosidase, leucine arylamidase,
and hydrolysis of esculin. It contained meso-diaminopimelic acid as the diagnostic dia-mino acid in the cell-wall. The predominant
isoprenoid quinone was menaquinone 7 (MK-7), and the major cellular fatty acids were anteiso-C 15:0 (52.9%), iso-Ci 16:0 (11.3%), and iso-C 15:0 (10.0%). The DNA G+C content was 43.3 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that this isolate
belonged to the family Paenibacillaceae. S22 T exhibited less than 97.0% 16S rRNA gene similarity with all relative type strains in the genus Paenibacillus, and the most closely related strains were Paenibacillus anaericanus MH21 T and Paenibacillus ginsengisoli Gsoil 1638 T, with equal similarities of 95.8%. This polyphasic evidence suggested that strain S22 T should be considered a novel species in the genus Paenibacillus, for which the name, Paenibacillus pini sp. nov., is proposed. The type strain is S22 T (=KCTC 13694 T =KACC 14198 T =JCM 16418 T) 相似文献
12.
A red-pigmented, Gram-negative, strictly aerobic, rod-shaped bacterium which was motile by gliding, designated strain 1351 T, was isolated from the soil of Lengduo, Tibet in China and subjected to a polyphasic taxonomic analysis. The isolate grows
optimally at 30°C and pH 7. It grows with NaCl tolerated up to 1.5% (optimum, 0.5%). Phylogenetic analysis based on the 16S
rRNA gene sequence shows that strain 1351 T is closely related to members of the family ‘ Cytophagaceae’ closest sequence similarity was observed with similarity with Adhaeribacter terreus (91.8%). The major whole-cell fatty acids are summed feature 4 (containing anteiso-C 17:1 B and/or iso-C 17:1 I, 29.2%), summed feature 3 (containing C 16:1ω6 c and/or C 16:1ω7 c, 13.0%) and iso-C 15:0 (12.0%). The predominant menaquinone of strain 1351 T is menaquinone-7 (MK-7) and the G+C content of the DNA is 46.8 mol%. According to the phylogenetic evidence and phenotypic
data, strain 1351 T is considered to represent a new genus and species of the family ‘ Cytophagaceae’ for which the name Rufibacter tibetensis gen nov., sp. nov. is proposed. The type species is R. tibetensis and the type strain is 1351 T (=CCTCC AB 208084 T = NRRL B-51285 T). 相似文献
13.
Strain M1-2 T was isolated from the black sand from the seashore of Jeju Island, Republic of Korea and was classified using a polyphasic
taxonomic approach. Strain M1-2 T appeared as Gram-negative, motile rods that could grow in the presence of 1–10% (w/v) NaCl and at temperatures ranging from
4 to 37°C. This isolate has catalase and oxidase activity and hydrolyses aesculin, DNA and l-tyrosine. Based on phylogenetic analysis using 16S rRNA gene sequences, strain M1-2 T belongs to the genus Joostella and is clearly distinct from the other described species of this genus, Joostella marina (type strain En5 T). The 16S rRNA gene sequence similarity level between M1-2 T and J. marina En5 T is 97.2%, and the DNA–DNA relatedness value between the two strains is 23.9%. Strain M1-2 T contains MK-6 as the major menaquinone and iso-C 15:0, summed feature 3 (C 16:1 ω7c and/or iso-C 15:0 2OH) and iso-C 17:0 3OH as major cellular fatty acids. The DNA G + C content is 32.3 mol%. These data suggest that strain M1-2 T should be classified as a novel species, for which the name Joostella atrarenae sp. nov. is proposed. The type strain for the novel species is M1-2 T (= KCTC 23194 T = NCAIM B.002413 T). 相似文献
14.
Two novel, Gram-positive, motile, coccal bacteria, strains L1b-b9 T and B5a-b5, were isolated from a potato cultivation field in Ochang, Korea. These isolates grew at 10–45°C, pH 5.0–10.0,
and in the presence of 8% (w/v) NaCl. The diagnostic diamino acid in the cell-wall peptidoglycan was meso-diaminopimelic acid. The major menaquinone was MK-8(H 4) and the main cellular fatty acids were iso-C 14:0, iso-C 15:0, and anteiso-C 15:0. Polar lipids in strain L1b-b9 T consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, and an unknown glyco-amino lipid. The G+C
content of genomic DNA was 73.6 mol%. A phylogenetic analysis based on 16S rRNA gene sequences showed that strains L1b-b9 T and B5a-b5 shared 99.36% similarity and formed a robust clade with the type species of the genus Phycicoccus. Strain L1b-b9 T is related most closely to Phycicoccus cremeus V2M29 T (97.52% 16S rRNA gene sequence similarity). On the basis of phylogenetic characteristics, the name Phycicoccus ochangensis sp. nov. is proposed for strain LIb-b9 T (=KCTC 19694 T =JCM 17595 T). 相似文献
15.
Two bacterial strains, KIS66-7 T and 5GH26-15 T, were isolated from soil samples collected in the South Korean cities of Tongyong and Gongju, respectively. Both strains were aerobic, Gram-stain-positive, mesophilic, flagellated, and rodshaped. A phylogenetic analysis revealed that both strains belonged to the family Microbacteriaceae of the phylum Actinobacteria. The 16S rRNA gene sequence of strain KIS66-7 T had the highest similarities with those of Labedella gwakjiensis KSW2-17 T (97.3%), Cryobacterium psychrophilum DSM 4854T (97.2%), Leifsonia lichenia 2Sb T (97.2%), Leifsonia naganoensis JCM 10592 T (97.0%), and Cryobacterium mesophilum MSL-15 T (97.0%). Strain 5GH26-15 T showed the highest sequence similarities with Leifsonia psychrotolerans LI1T (97.4%) and Schumannella luteola KHIAT (97.1%). The 16S rRNA gene sequence from KIS66-7 T exhibited 96.4% similarity with that from 5GH26-15 T. Strain KIS66-7 T contained a B2γ type peptidoglycan structure with D-DAB as the diamino acid; MK-13, MK-12, and MK-14 as the respiratory quinones; ai-C 15:0, ai-C 17:0, and i-C 16:0 as the major cellular fatty acids; and diphosphatidylglycerol, phatidylglycerol, and glycolipids as the predominant polar lipids. Strain 5GH26-15T had a B2β type peptidoglycan structure with D-DAB as the diamino acid; MK-14 and MK-13 as the respiratory quinones; ai-C 15:0, i-C 16:0, and ai-C{vn17:0} as the major cellular fatty acids; and diphosphatidylglycerol, phatidylglycerol, and glycolipids as the predominant polar lipids. Both strains had low DNA-DNA hybridization values (<40%) with closely related taxa. Based on our polyphasic taxonomic characterization, we propose that strains KIS66-7 T and 5GH26-15 T represent novel genera and species, for which we propose the names Diaminobutyricibacter tongyongensis gen. nov., sp. nov. (type strain KIS66-7 T=KACC 15515 T=NBRC 108724 T) and Homoserinibacter gongjuensis gen. nov., sp. nov. (type strain 5GH26-15 T=KACC 15524 T=NBRC 108755 T) within the family Microbacteriaceae. 相似文献
16.
A novel Gram-staining positive, aerobic, rod-shaped, non-motile and yellow-pigmented actinobacterium, designated strain WY83T, was isolated from a marine sediment of Indian Ocean. Strain WY83T grew optimally at 30–35 °C, pH 7–8 and with 0–3% (w/v) NaCl. The predominant menaquinones were MK-10, MK-11 and MK-12, and the major fatty acids were C19:1 ω9c/C19:1 ω11c, anteiso-C15:0, C17:0 3OH, and iso-C16:0. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol and one unidentified glycolipid. The cell-wall peptidoglycan contained lysine as a diamino acid. The DNA G?+?C content was 72.3 mol%. Phylogenetic analysis based on 16S rRNA gene sequences and ninety-two bacterial core genes indicated that strain WY83T formed an evolutionary lineage with Chryseoglobus frigidaquae JCM 14730T, Chryseoglobus indicus CTD02-10-2T, Yonghaparkia alkaliphila JCM 15138T, Microcella alkaliphila DSM 18851T and Microcella putealis DSM 19627T within the radiation enclosing members of the family Microbacteriaceae. All pairwise percentage of conserved proteins between strain WY83T and the closely related phylogenetic neighbors were greater than 65%. The average nucleotide identity and in silico DNA–DNA hybridization values were both below the thresholds used for the delineation of a new species. On the basis of the evidence presented, strains WY83T, Y. alkaliphila JCM 15138T, C. frigidaquae JCM 14730T, M. alkaliphila DSM 18851T and M. putealis DSM 19627T should belong to different species of the same genus. Strain WY83T represents a novel species of the genus Microcella, for which the name Microcella flavibacter sp. nov. is proposed. The type strain is WY83T (=?KCTC 39637T?=?MCCC 1A07099T). Furthermore, Chryseoglobus frigidaquae, Chryseoglobus indicus, and Yonghaparkia alkaliphila were reclassified as Microcella frigidaquae comb. nov., Microcella indica nom. nov., and Microcella alkalica nom. nov., respectively. 相似文献
17.
A Gram-negative, motile and rod-shaped bacterial strain, designated S7-3 T, was isolated from a tidal flat sediment at Saemankum on the western coast of Korea. Phylogenetic analyses based on 16S rRNA gene and gyrB sequences showed that strain S7-3 T belonged to the genus Shewanella, clustering with Shewanella decolorationis S12 T. Strain S7-3 T exhibited 98.8 % 16S rRNA gene sequence similarity and 96.8 % gyrB sequence similarity to S. decolorationis S12 T, respectively. The 16S rRNA gene sequence similarity values between strain S7-3 T and other members of the genus Shewanella were in the range of 93.0–98.0 %. Strain S7-3 T contained simultaneously both menaquinones (MK) and ubiquinones (Q); the predominant menaquinone was MK-7 and the predominant ubiquinones were Q-7 and Q-8. The fatty acid profiles of strain S7-3 T and S. decolorationis JCM 21555 T were similar; major components were C 17:1 ω8 c, iso-C 15:0 and iso-C 15:0 2-OH and/or C 16:1 ω7 c. The DNA G+C content of strain S7-3 T was 51.8 mol% and its mean DNA–DNA relatedness value with S. decolorationis JCM 21555 T was 43 %. Differential phenotypic properties of strain S7-3 T, together with the phylogenetic and genetic distinctiveness, revealed that this strain is distinguishable from recognized Shewanella species. On the basis of the data presented, strain S7-3 T is considered to represent a novel Shewanella species, for which the name Shewanella seohaensis sp. nov. is proposed. The type strain is S7-3 T (=KCTC 23556 T = CCUG 60900 T). 相似文献
18.
A Gram-positive bacterium, designated M-GX18 T, was isolated from the rhizosphere soil of mango (Guangxi Province, China). The isolate produced nematicidal volatile compounds
with activities against the root-knot nematode Meloidogyne incognita. The organism was motile, catalase- and oxidase-positive, spore-forming and rod shaped. The predominant menaquinone was MK-7
and the major cellular fatty acid profiles were iso-C 15:0, anteiso-C 15:0 and iso-C 16:0. The DNA G+C content was 38.9%. A phylogenetic analysis based on 16S rRNA gene sequences showed that this organism represented
a new species of the genus Lysinibacillus. Strain M-GX18 T exhibited high 16S rRNA gene sequence similarity with its closest neighbors Lysinibacillus sphaericus (98.5%), Lysinibacillus fusiformis (98.1%) and Lysinibacillus xylanilyticus (98.6%). The physiological, biochemical and chemotaxonomic data, including DNA–DNA hybridization relatedness data, indicate
that strain M-GX18 T can be distinguished from all the related species of the genus Lysinibacillus. Therefore, on the basis of the polyphasic taxonomic data presented, a new species of the genus Lysinibacillus, Lysinibacillus mangiferahumi, with the type strain M-GX18 T (=DSM 24076 T = CCTCC AB 2010389 T) is proposed. 相似文献
19.
A marine bacterium, designated IMCC3175 T, was isolated from a seawater sample collected off the Antarctic coast. The strain was Gram-negative, obligately aerobic,
carotenoid pigment-containing, and rod-shaped bacterium that divided by binary fission. As determined by 16S rRNA gene sequence
comparisons, the most closely related genera were Formosa (92.9∼93.3%), Bizionia (91.6∼93.2%), Gaetbulibacter (91.5∼92.8%), Sediminibacter (92.7%), Yeosuana (92.6%), Subsaximicrobium (92.1∼92.2%), and Gillisia (89.5∼92.2%). Phylogenese analysis based on 16S rRNA gene sequences showed that the strain formed a monophyletic clade together
with the genera Sediminibacter and Subsaximicrobium but represented an independent phyletic line in this clade of the family Flavobacteriaceae. The DNA G+C content of the strain was 37.3 mol%. The major respiratory quinone was MK-6 and the predominant cellular fatty
acids were C 16:1 ω7c and/or iso-C 15:0 2-OH (12.8%), anteiso-C 15:0 (9.4%), and iso-C 16:1 (9.4%). Low 16S rRNA gene sequence similarity, formation of a distinct phylogenetic branch, and several phenotypic characteristics,
including a narrow range of temperature and salinity for growth, differentiated strain IMCC3175 T from other related genera in the family Flavobacteriaceae. Therefore the name Antarcticimonas flava gen. nov., sp. nov. is proposed, with strain IMCC3175 T (=KCCM 42713 T =NBRC 103398 T) as the type strain. 相似文献
20.
A novel actinomycete, designated as strain YIM 75980 T, was isolated from a soil sample collected from a dry-hot river valley in Dongchuan county, Yunnan province, south-west China
and was subjected to polyphasic taxonomic characterization. The organism produced circular, smooth, red to black coloured
colonies comprising coccoid-shaped cells. Colonies on agar medium lacked mycelia and cells adhered to the agar. Strain YIM
75980 T contained meso-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan and contained galactose, arabinose and
glucosamine as the main sugars in the whole-cell hydrolysates. The predominant menaquinone was MK-9 (H 4) and the major fatty acids were iso-C 15:0, iso-C 16:0 and C 16:0. The DNA G + C content of strain YIM 75980 T was 73.1 mol%. Phylogenetic analyses based on 16S rRNA gene sequences clearly showed that strain YIM 75980 T formed a distinct clade within the genus Geodermatophilus and was closely related to Geodermatophilus obscurus DSM 43160 T (level of similarity, 97.9%). Furthermore, the result of DNA–DNA hybridization between strain YIM 75980 T and G. obscurus 43160 T demonstrated that this isolate represented a different genomic species in the genus Geodermatophilus. Moreover, the physiological and biochemical data showed the differentiation of strain YIM 75980 T from its closest phylogenetic neighbour. Therefore, it is proposed that strain YIM 75980 T represents a novel species of the genus Geodermatophilus, for which the name Geodermatophilus
nigrescens sp. nov. is proposed. The type strain is YIM 75980 T (=CCTCC AA 2011015 T =JCM 18056 T). 相似文献
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