刺齿虫兆属温州一新种及浙江分种检索表（弹尾纲：长角虫兆科）

记述刺齿虫兆属温州 1 新种:六毛刺齿虫兆Homidia hexaseta sp. nov.,该种的鉴别特征包括体色,下唇具光滑毛L1,头背部顶区具6根大刚毛,胸部第Ⅱ节具p4系列大刚毛,腹部第Ⅲ节中间具a2大刚毛,腹部第Ⅳ节具B6和Ae5-7大刚毛以及端节亚顶端齿远大于顶端齿。同时,本文还给出了浙江省刺齿虫兆属分种检索表。正模:♀,浙江省温州市泰顺县乌岩岭国家自然保护区,采集号C9271-4,2005-Ⅶ-29;副模:5♀♀。其中两头雌性副模标本保存于台州学院生命科学学院,其余标本保存于南京大学生命科学学院。     

浙江省大雷山刺齿虫兆属一新种描述（弹尾纲：长角虫兆科）

记述浙江省大雷山刺齿虫兆属 1 新种,张氏刺齿虫兆Homidia zhangi sp. nov., 该种鉴别特征有体色,光滑下唇毛 L1,腹部第 4 节后侧中间 3+3 根大刚毛及长度相近的特化毛,腹部第 1 节和第 5 节特化毛的相对位置以及腹管侧瓣和弹器端区的大量纤毛状刚毛。模式标本保存于南京大学生命科学学院和台州学院生命科学学院。     

刺齿(虫兆)属温州—新种及浙江分种检索表(弹尾纲:长角(虫兆)科)

记述刺齿(虫兆)属温州1新种:六毛刺齿(虫兆) Homidia hexaseta sp.nov.,该种的鉴别特征包括体色,下唇具光滑毛L1,头背部顶区具6根大刚毛,胸部第Ⅱ节具p4系列大刚毛,腹部第Ⅲ节中间具a2大刚毛,腹部第Ⅳ节具B6和Ae5-7大刚毛以及端节亚顶端齿远大于顶端齿.同时,本文还给出了浙江省刺齿(虫兆)...     

浙江东白山裸长角虫兆属一2+2单眼新种（弹尾纲：长角虫兆科）

记述裸长角虫兆属1有眼新种：横眼裸长角虫兆Sinella transoculata sp. nov.。该新种的鉴定特征有2+2横向排列单眼,身体背部（除腹部第V–VI节）散布有棕色斑点,腹部第II节具a2大刚毛,腹部第IV节后侧中间具3根大刚毛及侧面具5根大刚毛。文中给出了该新种的特征图及该属2+2单眼横向排列物种之间的特征差异。     

福建省刺齿虫兆属（弹尾纲：长角虫兆科）一新种描述

记述福建省刺齿属1新种:拟中华刺齿Homidia pseudosinensis sp.nov.。该新种的鉴定特征有胸部第2节后侧具近似M型的横条带,下唇毛序及腹部第4节毛序。本文提供了该物种的特征图及福建省刺齿属分种检索表。     

中国西部刺齿(虫兆)属Homidia一新种(弹尾纲:长角(虫兆)科)

本文记述中国西部剌齿(虫兆)属1新种:中带剌齿(虫兆)H.mediofascia,sp.nov..该种区别属内其他种的主要特征是本种胸部第2节到腹部第2节背部中间具色带,第1腹节具大刚毛a1,腹部第4节后缘大刚毛7+7,粘管后侧面具光滑刚毛4根.本文还对属内5个相近种进行了比较.正模:早,陕西省安康市,2006-Ⅵ-15;副模:8♀♀,1♂以及8头酒精标本,陕西省安康市,2006-Ⅵ-15.1♀和1头酒精标本保存于南京大学,其他标本保存于台州学院生命科学学院.     

浙江省洞头拟裸长角虫兆属一新种（长角虫兆科：弹尾纲）

记述浙江省洞头岛拟祼长角虫兆属1无眼新种,岛屿拟裸长角虫兆Coecobrya islandica Shi&Pan sp.nov.。此新种的鉴定特征为无眼,弹器基具"光滑"刚毛,胫胕节内侧无"光滑"刚毛,下唇MREL1L2为光滑刚毛,X和X4为光滑刺状小刚毛,上唇基刚毛,腹部第II–IV节的感觉毛以及背部毛序。该新种与短毛拟裸长角虫兆Coecobrya brevis Xu et al.,2012最相似。本文给出了该新种的特征图及与相似种的详细特征比较。模式标本保存于台州学院生命科学学院和南京农业大学植保学院昆虫系。     

福建沼虾属二新种

1975年,我们在福建省收集到许多淡水虾的标本,发现沼虾属有二个新种,记述于下。福建沼虾Macrobrachium fukienense,新种 额角上缘稍隆起,末端超出第一触角柄,上缘具7或8齿,基部1—2齿位于眼眶后方的头胸甲上;下缘凸,具2(少数仅1)齿。头胸甲具颗粒状突起,雄性尤为显著(图1),腹部的颗粒小而分散。尾节(图7)背面具2对背刺,后端呈尖刺状,后侧角具2对刺,中部具6—7对羽状刚毛,后缘的背面具5对细刚毛。 第一触角柄刺短于第1节的1/2,前例刺伸至第2节的中部,第2节稍短于第3节。鳞片(图 3)长约为宽的2.5—2.8倍。     

四川省的桡足类(中)

十八、棕色大剑水蚤Macrocyclopsfuscus(Jurine)雌性第4胸足内肢第3节内缘具羽状刚毛二根,末缘的外刺约为内刺长度的1.7倍。生活于池塘和稻田。采自宜宾和乐山。十九、闻名大剑水蚤Macrocyclopsdistinctus(Richard)雌性第4胸足第3节内缘具羽状刚毛二根,末缘的外刺约为内刺长度的1.3倍。生活于池塘和稻田。采自南部、宜宾和乐山。为我省第一次记录。二十、白色大剑水蚤Macrocyclopsalbidus(Jurine)雌性第4胸足内肢第3节内缘具羽状刚毛和刺状刚毛各一根,羽状刚毛约为刺状刚毛长的4倍。生活于小河和池塘。采自泸县和纳溪。二十一、寡毛大剑…     

米氏球角跳属中国新纪录及一新种(弹尾目,球角跳科)

报道了球角跳科在中国的1新纪录属"米氏球角跳属",并描述了1新种,新种命名为鞍山米氏球角跳Mitchellania anshanensis sp.nov.鞍山米氏球角跳与描述自西伯利亚的M.subhorrida Babenko,1994相似,两者的主要区别在于:新种体长仅0.8mm,腹部第5节背面p1毛之间体表颗粒数量为17～20,触角第4节腹面有20～25个弯曲且顶端膨大的短小感觉毛,爪部生有1内齿,但无侧齿,胸部第2和第3节背面感觉毛短小精巧,腹部第1～3节背面感觉毛与普通刚毛长度相同;M.subhorrida体长达到1.5mm,腹部第5节背面p1毛之间体表颗粒数量为8～10,触角第4节腹面弯曲且顶端膨大的短小感觉毛数量范围为30～35个,爪部生有1内齿和1侧齿,胸部第2节、腹部第1～3节背面感觉毛与普通刚毛长度相同,胸部第3节和腹部第2节背面感觉毛短小粗壮.模式标本保存在中国科学院上海植物生理生态研究所.     

六种藓类植物茎的比较解剖学研究

通过对6种藓类植物,即褶叶青藓(Brachythecium salebrosum(Web.et Mohr.)B.S.G.)、湿地匐灯藓(Plagiomnium acutum(Lindb.)Kop.)、侧枝匐灯藓(Plagiomnium maximoviczii(Lindb.)Kop.)、大凤尾藓(Fissidensnobilis Griff.)、大羽藓(Thuidium cymbifolium(Doz.et Molk.)B.S.G.)和大灰藓(Hypnum plumaeforme Wils.)嫩茎和老茎的石蜡切片和显微观察发现,同一藓类植株的嫩茎和老茎,茎结构稳定,不同种藓类植物茎横切面具有不同特征.植物体茎横切面形状、表层细胞的层数、细胞大小和细胞壁厚薄、皮层细胞大小和形状、中轴的有无以及比例等特征可以作为藓类植物的分科分类依据之一.     

Cause of Senescence of Nine Sorts of Flowers

The levels of endogenous phytohormones and respiratory rate in nine sorts of flowers such as Cymbidium faberi Rolfe, Nopalxochia ackermannii Kunth and others were investigated both at full bloom and senescence and meanwhile the effect of exogenous phytohormones on prolonging the blossoms and promoting ethylene production were tested. There is a high content of endogenous ethylene in all the long-lived flowere, about 3–16 folds higer than the short-lived ones. There is a high level of ABA at full blooming flowers of short-lived flowers, in which there is no or only some cytokinins in it, but the ratio of CTK (6BA+zeatin)/ABA is smaller(l.7). The endogenous ABA reached a much higher level at senescence in all nine sorts of flowers, so it is reasonable to consider that it is ABA which plays an important role of regulation in controlling flower's senescence. There is a much higher level of GA3 and zeatin in the long-lived flowers which is not demonstrated in the shortlived ones. The respiratory rate is one of the factors controtling the longevity of flowers, but it does not play a decided role. Application of 6BA and zeatin prolongs distinctly orchid’s longevity, however exogenous IAA through the promotive action on ethylene production, evidently extends the longevity of the flowers of the Nopalxochia ackermannii Kunth.     

龙胆科药用植物化学成分的研究现状

龙胆科植物在我国的分布范围很广,且多数为药用植物,其多数种属的药用植物,至今其化学成分尚未被系统研究。综述了目前龙胆科药用植物的化学成分的研究现状及一般提取方法,对近年来发现的环烯醚萜及裂环烯醚萜类化合物进行了总结,为本科药用植物的更深入研究提供了参考。     

The effects of glutamine of the maintenance of embryogenic cultures of Cryptomeria japonica

Summary Embryogenic tissues of sugi (Cryptomeria japonica) were induced on a modified Campbell and Durzan (CD) medium containing 1 μM 2,4-dichlorophenoxyacetic acid (2,4-D) and 600 mg l⁻¹ glutamine, and subcultured in the medium of the same composition for over 1 yr. This resulted in a mixed culture of embryogenic and non-embryogenic cells. When embryogenic cells were isolated and cultured independently, their capacity to form embryogenic aggregates was lost. Thus, the non-embryogenic cells present within a mixed culture system were essential to the formation of embryogenic aggregates. When embryogenic tissues were isolated and cultured independently on a high glutamine-containing (2400 mg l⁻¹) medium, dry weights and endogenous levels of glutamine increased, and the tissue could generate a large number of embryogenic aggregates. Amino acid analysis of embryogenic and non-embryogenic cells from the maintenance culture indicated a higher level of glutamine was present in the latter. The high endogenous level of glutamine in the non-embryogenic portion of mixed cell masses may be the supplier of glutamine for maintaining the embryogenic property of the tissues.     

Specificity of action of piperidylcholine derivatives as substrates of cholinesterases of various origin

The review deals with study of enzymologic properties of a novel highly specific acetylcholinesterase substrate, N-(β-acetoxyethyl) piperidinium iodomethylate (“piperidylcholine”), and its 30 derivatives that were tested as effectors of cholinesterases of mammals and various species of Pacific squids. It was proven for the first time that responsible for specificity of action was structure of cyclic ammonium grouping of the alcohol part of molecule of the ester substrate. Analysis of specificity is performed based on enzymatic hydrolysis parameters—activity of catalytic center of cholinesterases and bimolecular constant of the reaction rate that are determined at optimal and low substrate concentrations. Among the specially synthesized group of thioester compounds there is revealed one more highly specific acetylcholinesterase substrate—N-(β-acetoxyethyl) piperidinium.     

真菌类遗传学分析的知识结构教学

本文以认知结构理论为指导,讨论了真菌类遗传分析与高等动植物遗传分析的内在联系,认为利用这种内在联系进行教学可收到好的效果并说明了作者的具体教学过程。 Abstract:In the paper, the relationship between genetic analysis of Fungi and genetic analysis of high animal and plant was discussed.A good results were obtained when we adopted this method in the teaching.     

医疗机构合理用药评价模型的构建研究

目的 针对医疗机构的合理用药水平进行评价研究。方法 根据医疗机构合理用药的具体要求,构建医疗机构合理用药评价指标体系,采用基于模糊群决策的方法和多指标评价分析法构建医疗机构合理用药评价模型。结果 构建了基于模糊群决策的医疗机构合理用药评价模型,并通过实例分析证明了评价模型的可行性。结论 建立的基于模糊群决策的医疗机构合理用药评价模型能够对医疗机构的合理用药水平进行科学评价,为提高医疗机构合理用药水平奠定基础。     

棉铃虫钙粘蛋白N端多肽多克隆抗体制备及对Bt抗性的初步检测

用重组表达的棉铃虫Helicoverpa armigera(Hübner)中肠钙粘蛋白N端多肽片段制备兔多克隆抗体,并利用其对Bt抗性进行鉴定。通过RT-PCR方法对棉铃虫中肠钙粘蛋白N端多肽的基因片段Cad285进行PCR扩增,将其克隆到pET-30a原核表达载体中,在大肠杆菌BL21(DE3)中经IPTG诱导表达,得到35ku的重组融和蛋白,融合表达的包涵体经过变性、Ni-NTA柱亲和纯化、复性等方法处理包涵体,获得可溶性纯化蛋白,用纯化后蛋白免疫新西兰兔制备多克隆抗体,ELISA检测其效价高于1∶16000;利用最终获得的多克隆抗体对室内纯合Bt抗/感品系的棉铃虫中肠钙粘蛋白进行Western blot分析,结果显示敏感和抗性品系之间有明显差异,表明其能够应用对Bt抗性进行初步检测。