Discovery of a Homolog of Siderophilin in a Plant

Members belonging to the siderophilin family are iron-binding and iron-transporting proteins, which includes transferrin and lactoferrin. They have only been found in animals previously. If siderophilin could be found in and isolated from a plant, its production and subsequent extensive application could be increased. The present study is the first to report the discovery of a homolog of siderophilin in a plant. In order to purify antifreeze proteins from Ammopiptanthus mongolicus （Maxim.） Cheng f., the authors processed the proteins from the leaves using techniques such as column chromatography using DEAE-Cellulose-52, gel filtration via Sephacryl S-100 HR medium, hydrophobic interaction chromatography, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Mass spectroscopy was performed on the three proteins purified and the sequence of one of the proteins （containing 32 amino acids） was found to have 97% homology with the corresponding part of one type of human lactoferrin. Moreover, one of the two peptides belongs to an iron-binding domain. So, it is possible that siderophilin also exists in plants and plays a role as an antibacterial and antifungal, among other actions.     

Dynamics of a mutualism in a multi-species context

Despite recent findings that mutualistic interactions between two species may be greatly affected by species external to the mutualism, the implications of such multi-species interactions for the population dynamics of the mutualists are virtually unexplored. In this paper, we ask how the mutualism between the shoot-base boring weevil Apion onopordi and the rust fungus Puccinia punctiformis is influenced by the dynamics of their shared host plant Cirsium arvense, and vice versa. In particular, we hypothesized that the distribution of the weevil's egg load between healthy and rust-infected thistles may regulate the abundance of the mutualists and their host plant. In contrast to our expectations we found that the dynamics of the mutualists are largely determined by the dynamics of their host. This is, to our knowledge, the first demonstration that the dynamics of a mutualism are driven by a third, non-mutualistic species.     

Evolution of a Langmuir wave in a weakly inhomogeneous plasma in a longitudinal electric field

The spatial evolution of a Langmuir wave excited by external sources in a weakly inhomogeneous electron plasma in a longitudinal electrostatic field is considered. It is shown that, in a longitudinal electrostatic field, a Langmuir wave can only be amplified in an inhomogeneous plasma provided that the current of trapped electrons exceeds that of untrapped electrons. In this case, as the wave propagates through the inhomogeneous region where its phase velocity increases, some untrapped electrons become trapped in the wave potential wells. As a result, the current of trapped electrons increases and the wave is amplified. Moreover, in the regions where the bulk electrons are localized, the minima of the wave are amplified to a greater extent than its maxima.     

Decay instability of a laser pulse propagating in a transverse direction in a magnetized plasma

The decay instability of a lser pulse propagating across an external magnetic field in a subscritical plasma is investigated analytically and numerically. It is shown that, when the relaxation of the pulse is taken into account, the hydrodynamic growth rate of the decay instability is slower than that obtained earlier in the constant-amplitude pump wave approximation. The results of numerical simulations by a particle-in-cell method demonstrate that an increase in the amplitude of the parametrically excited waves is accompanied by a decrease in their group velocity; in this case, up to 85% of the laser energy is converted into the energy of the plasma particles. It is found that, under resonance conditions, the magnetic field acts to increase the energy of the accelerated ions that escape from the plasma slab through its front boundary.     

Prostatectomy in a county hospital; a review of 677 cases in a six-year period

Transurethral procedures were used in 620 of 677 cases in which prostatectomy was done (principally by residents supervised by a urologist) at a county hospital in a six-year period. Open operations were used in the other 57 cases. Results were classified as "excellent" in 46.8 per cent of the transurethral cases and as "good" in 36.3 per cent.     

Mechanisms of coexistence of a bacteria and a bacteriophage in a spatially homogeneous environment

When bacteriophage are added to laboratory bacteria populations, bacteria mutants that are resistant to the phage quickly dominate the population. The phage will only persist in the long‐term if there are sufficient bacteria in the population that show susceptibility to the phage. We investigated the mechanisms allowing for coexistence by adding the virulent bacteriophage φ6 to cultures of the bacterium Pseudomonas syringae pv. phaseolicola in a spatially homogeneous environment. We saw large differences between replicate cultures, in particular when one or both of the species persisted. These differences can be explained by variation in the timing of the appearance of various resistant phenotypes in the bacteria populations before the phage were added, which determines their relative frequencies within the populations. Although these resistant phenotypes have similar fitnesses in the presence and in the absence of the phage, they have a profound effect on the persistence of the phage. Our results give a clearer understanding of the ecological mechanisms that lead to the coexistence of bacteria and virulent phage in environments where there are no spatial refuges available to the bacteria population.     

LCA of a tomato crop in a multi-tunnel greenhouse in Almeria

Purpose

Protected crops have expanded significantly in the Mediterranean area over the last few decades as a successful means to provide abundant and high-quality produce. Although resources are generally used efficiently, greenhouse areas cause major environmental impacts. The aim of this work was to study, from an environmental point of view, the improvement capacity of greenhouse areas in the Mediterranean region and to assess several alternative agricultural practices to decrease their contribution to the environmental impacts in this system.

Materials and methods

The methodology used was life cycle assessment (LCA) based on a tomato crop grown in a multi-tunnel greenhouse in Almeria, on the southeast coast of Spain. The functional unit chosen was 1 ton of loose classic tomatoes. Five midpoint impact categories and one energy flow indicator were selected for their relevance. The agricultural practice alternatives evaluated were reduction of volume of substrate and fertilizers, extension of substrate and greenhouse life span and increase in renewable energy for electricity production.

Results and discussion

The results indicated that the main contributors to impact categories in the tomato production were structure, auxiliary equipment and fertilizers. Structure accounted for between 30 and 48?% of the contributions, depending on the impact category. The principal burdens in the auxiliary equipment stage were substrate and consumption of electricity. Fertilizers environmental impacts were due to emissions during their manufacture and application to the crop. In a best-case option, taking into account the best alternatives, contributions to the impact categories were reduced by between 17 and 30?%. The LCA methodology proved to be a useful tool to evaluate the environmental damage of this agricultural activity. The importance of including farm infrastructure in the assessment was demonstrated as it was a major contributor. The risk of eutrophication could be reduced by adjustment of the fertilizers?Cwater balance and implementation of a closed-loop irrigation system. Future technological improvements should be developed to increase yields and thereby directly reduce the environmental burdens per unit produce.

Conclusions

The present study served to assess the environmental impacts of a tomato crop in a multi-tunnel greenhouse on the coast of Almeria. The assessment was used to evaluate alternatives for improvement of cleaner production in greenhouse areas. Further research should focus on assuring the feasibility of the suggested options.     

Diapause in a large aggregation of a tropical beetle

Abstract. 1_˜ Diapause is described for adults of Stenotarsus rotundus Arrow (Endomychidae, Coleoptera) in the tropical lowland forest on Barro Colorado Island, Panama.
2 . Diapause lasted up to 10 months each year, covering some 6 months of the wet season plus 4 months of the dry season. When in diapause the beetles clustered in an aggregation of up to 70 ,000 individuals on the stem of a palm tree, the same individual palm tree in at least four successive years.
3. The habitat and habits of the beetle when not in diapause are unknown, but one or two generations may be produced during this period. Beetles returned to the diapause site from June until late August.
4. Soon after arrival at the diapause site the flight muscles degenerated and did not develop again until February/March. The ovaries were undeveloped when the beetles arrived at the site and remained in that condition until the onset of yolk deposition in March. At the beginning of the rainy season in April the beetles mated and dispersed.
5. Lean dry weight of the beetles did not decrease noticeably during diapause, but fat content did. It is suggested that the beetles do not feed, or feed very little, while in diapause. During diapause, the metabolic rate is surprisingly low considering a mean ambient temperature of 25° C.
6. Juvenile hormone analogue (ZR515) stimulated flight muscle and ovary development. Daylength, when different from LD 12: 12 , initiated a partial flight muscle development, but there was no effect on the ovaries. Field observations suggest that neither temperature nor humidity had an effect on the termination of diapause.
7. Although the development of both flight muscles and ovaries were stimulated by juvenile hormone analogue and both developed around March of each year, it is suggested that the mechanisms causing this development are different.     

Production of a fish taxocene in a Texas pond

Synopsis Production rates of seven species of fishes were determined for one year in a 0.95 ha pond. Total annual production of all fishes in the pond was 209 g m^–2y^–1 (after the subtraction of 16.2 g m^–2y^–1 of negative production). Over 90% of the production occurred in the months of June through September, with young-of-the-year fishes contributing 84% of the total. When Y of Y fishes shifted to benthic diets in the spring, the small biomass of benthos available could not sustain the large biomas of fish. Severe competition for the available food then resulted in decreased production rates for the entire fish taxocene.     

Transport processes in a sound of a large lake

There are some problems in modelling the water exchange between a bay and the main body of a large lake. The objective of this study was to clarify which processes are important in the water exchange and must be taken into account in modelling. The study was mainly based on current measurements in the outlet of a bay system (Tiirinselkä-Lehesselkä) of lake Päijänne with an acoustic current profiler and recordings of temperature profile. High currents in the hypolimnion were observed during periods of high stratification, when the flow in the hypolimnion is caused by the horizontal pressure gradient associated with the thermocline slope in the sound. In some cases the large water exchange was due to the seiches in main body of the lake. Water exchange and current velocity in the sound were clearly smaller when the water was homogeneous. This paper gives quantitative results of water exchange and its dependence on different factors for summer 1989.     

Refolding of a membrane protein in a microfluidics reactor

Membrane protein production for structural studies is often hindered by the formation of non-specific aggregates from which the protein has to be denatured and then refolded to a functional state. We developed a new approach, which uses microfluidics channels, to refold protein correctly in quantities sufficient for structural studies. Green fluorescent protein (GFP), a soluble protein, and bacteriorhodopsin (BR), a transmembrane protein, were used to demonstrate the efficiency of the process. Urea-denatured GFP refolded as the urea diffused away from the protein, forming in the channel a uniform fluorescent band when observed by confocal microscopy. Sodium dodecyl sulphate-denatured BR refolded within the channel on mixing with detergent–lipid mixed micelles. The refolding, monitored by absorbance spectroscopy, was found to be flow rate dependent. This potential of microfluidic reactors for screening protein-folding conditions and producing protein would be particularly amenable for high-throughput applications required in structural genomics. Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users.     

Evolution of a multi-agent system in a cyclical environment

The synchronisation phenomena in biological systems is a current and recurring subject of scientific study. This topic, namely that of circadian clocks, served as inspiration to develop an agent-based simulation that serves the main purpose of being a proof-of-concept of the model used in the BitBang framework, that implements a modern autonomous agent model. Despite having been extensively studied, circadian clocks still have much to be investigated. Rather than wanting to learn more about the internals of this biological process, we look to study the emergence of this kind of adaptation to a daily cycle. To that end we implemented a world with a day/night cycle, and analyse the ways the agents adapt to that cycle. The results show the evolution of the agents’ ability to gather food. If we look at the total number of agents over the course of an experiment, we can pinpoint the time when reproductive technology emerges. We also show that the agents adapt to the daily cycle. This circadian rhythm can be shown by analysing the variation on the agents metabolic rate, which is affected by the variation of their movement patterns. In the experiments conducted we can observe that the metabolic rate of the agents varies according to the daily cycle.     

Effect of a change in step direction from a forward to a lateral target in response to a sensory perturbation

The aim of the current study was to investigate the response of healthy older and young adults to a change in step direction from a forward to a lateral target in response to a sensory perturbation. Nine healthy older (75.1 ± 6.7 years; age range, 65–81 years) and nine young adults (27 ± 3.6 years; age range, 23–31 years) participated in the study. The sensory perturbation was a visual cue presented at random times while subjects stepped over an obstacle from quiet stance. For both young and elderly subjects there was an abrupt change in the slope of both shear ground reaction forces (GRFs) of the stance limb following the perturbation. The slope and peak of the change in GRFs was greater for the young subjects and the onset significantly earlier (205 ms compared to 271 ms). Changes in the GRFs were accompanied by an increase in bilateral gluteus medius and stance limb soleus activity. A late visual cue resulted in a delayed response for elderly subjects. These data show that a stepping response to a sensory perturbation was both delayed and of less magnitude for older adults which has implications for fall risk.     

Problems of being a cell in a soft body

Many soft bodied coelenterates are highly deformable or contractile. In the absence of hard skeletal elements, the epithelia are subjected to mechanical forces which cause a wide range of structural changes in the component epithelial cells. What kinds of structural change occur and how are the cells adapted to them? These questions are addressed with reference to cell surface area, cell membranes, cell junctions and epithelial cilia.