Studies on the Chemical Constituents of Rourea microphylla (Hook. et Arn) Planch.

Eleven compounds were isolated from the twigs and leaves of Rourea microphylla (Hook. et Arn) Planch. They were identified as quercetin-3-O-α-L-rhamnopyranoside (Ⅰ), hyperin (Ⅱ), quercetin (Ⅲ), astilbin (Ⅳ), β-sitosterol (Ⅴ), β-sitosteryl-β-D-glucopyranoside (Ⅵ), physcion (Ⅶ), erythroglaucin (Ⅷ), stearic acid (Ⅸ), palmitic acid (Ⅹ) and n-nonacosanane (Ⅺ) respectively, by means of UV, IR, NMR, MS spectra analyses and through chemical methods. The compounds Ⅰ, Ⅱ, Ⅲ, Ⅳ, Ⅵ, Ⅶ, Ⅷ, Ⅸ, Ⅹ and Ⅺ were first reportedin the Rourea genus.     

The Chemical Constituents of Antitumor from Sabina vulgaris Ant

Eleven known compounds, transcommunic acid (Ⅰ), cis-communic acid (Ⅱ), sandaracopimaric acid (Ⅲ), isocupressic acid (Ⅳ), sugiol (Ⅴ), 12-hydroxy-6, 7-seco- abieta-8, 11, 13-triene 6, 7-dial (Ⅵ), β-peltatin-A-methyl ether (Ⅶ), bergaptin (Ⅷ), β-sitosterol (Ⅹ), lignoceric acid (Ⅺ), cerotic acid(Ⅻ) and a new 3, 4-benzocumarins sabilactone (Ⅸ) were newly isolated from the bark of Sabina vulga ris Ant. (= Juniperus sabina L.). Their structures were determined from spectra data and chemical evidence. Compounds Ⅳ–Ⅹ were first isolated from this plant. The bioassay results showed that compounds Ⅰ–Ⅳ, Ⅵ andⅦ were shown to be cytotoxic effects for P-388 leukemia cell in vitro.     

银杏中种皮化学成分的分离及鉴定

银杏（Ginkgo biloba Linn．）在中国种植广泛,其产量占全世界总产量的70％[1]。中药白果仁是银杏种子去除肉质外种皮和骨质中种皮后得到的,具有敛肺定喘、止带缩尿的功效。目前,有关银杏种子的研究主要集中在具有药食同源的白果仁[2]及具有潜在应用价值的外种皮[3]上,鲜见对银杏种子中种皮的研究报道。生产中大量的银杏中种皮被废弃,因此,开展银杏中种皮的相关研究对于充分利用该类资源非常必要。鉴于此,作者对银杏中种皮的化学成分进行了系统分析,以期为其开发应用提供参考依据。     

The Chemical Constituents from Cynanchum Komarovii Al. Ilinski

Ten compounds were isolated from the roots and stems of Cynanchum komarovii Al. Iljinski. They were identified as 7-demethoxylophorine (Ⅰ), epi-alnusenol (Ⅱ), β-sitosterol (Ⅲ), triacontanolic acid (Ⅳ), sucrose (Ⅴ), desoxytylophorinin N-oxide (Ⅵ), apocynine (Ⅶ), paeonol (Ⅷ), β-sitosteryl-β-D-glucopyranoside (Ⅸ) and glucose (Ⅹ) by means of UV, IR, NMR, MS spectral analyses and through chemical methods. These compounds were first reported in this plant. One of them, Ⅵ is a new alkaloid. The bioassay results showed that the compounds Ⅰ, Ⅵ and reaction product Ⅺ exhibited cytotoxic effects to P-388 leukemia cell in vitro. Ⅺ is also a new compound.     

The Structure of Parvifolinoic Acid Isolated from Leaves of Isodon parvifolia

Eleven compounds were isolated from the leaves of lsodon parvifolia (Batalin) Hara. On the basis of spectral analysis and chemical methods, crystal Ⅳ was identified as a new compound, named parvifolinoic acid; Crystals Ⅴ–Ⅹ were identified as stearic acid (Ⅴ), β-sito- sterol (Ⅵ), daucosterol (Ⅶ), olenoic acid (Ⅷ), ursoric acid (Ⅸ), 2α-hydroxy-ursoric acid (Ⅹ) respectively. Primary pharmacology tests showed that crystal Ⅳ had inhibitory effect against QGY-7703.     

Separation and Identification of Chemical Constituents of Dysosma majorense(Gagn.)Hsiao et Y. H. Chen,nov comb

Ten compounds, namely physcion (Ⅰ), podophyllotoxone (Ⅱ), isopicropodophyllone (Ⅲ), picropodophyllone (Ⅳ), 4'-demethyl-podophyllotoxone (Ⅴ), dehydropodo- phyllotoxin (Ⅵ), diphyllin (Ⅶ), picropodophyllin (Ⅷ), podophyllotoxin (Ⅸ) and 4'-demethylpodophyllotoxin (Ⅹ), were separated from Dysosma majorense (Gagn.) Hsiao et Y. H. Chen, nov comb. Compound Ⅳ was first isolated from natural source and anthraquinone Ⅰ was first isolated from genus Dysosma. Ⅴ, Ⅷ and Ⅹ showed strong acttve inhibited against P388.     

The Chemical Constituents of Elsholtzia ciliata (Thund.) Hyland

Fourteen compounds have been obtained from the Elsholtzia ciliata (Thund.) Hyland. Their structures were identified by spectral and chemical methods as following: Ⅰ. 6-methyl-tritriacontane, Ⅱ. 13-cyclohexyl-hexoacosane, Ⅲ. β-sitosterol, Ⅳa. palmitic acid, Ⅳb. linoleic acid, Ⅳc. linolenic acid, Ⅴ. ursolic acid, Ⅵ. 5-hydroxy--6, 7-dimethoxyflavone, Ⅶ. 5-hyd-foxy-7, 8-dimethoxyflavone, Ⅷ. 5,7-dihydroxy-4'-methoxyflavone, Ⅸ. 5-hydroxy-7,4'-dime-thoxyflavone, Ⅹ.β-sitosterol-β-D-glucoside, Ⅺ. 5-hydroxy-6-methylflavanone-7-O-α-D-galacopy-ranoside, Ⅻ. acacetin-7-O-β-glucoside. Among all the compounds, Ⅺ is a new compound. Except for Ⅳb and Ⅳc, the other twelve compounds were first found in this plant.     

Studies on the Alkaloids of Stephania yunnanensis

Ten known alkaloids, sinoacutine (Ⅰ), 1-tetrahydropalmatine (Ⅱ), d-stepharine (Ⅲ), 1- stepholidine (Ⅳ), 1-corydalmine (Ⅴ), stephanine (Ⅵ), palmatine (Ⅶ), dehydrocorydalmine (Ⅷ), stepharanine (Ⅸ) and roemerine (Ⅹ) were isolated from the roots of Stephamia yunnanensis H. S. Lo. Their structures were determined from spectral data and chemical evidence.     

小叶黄杨化学成分的研究

小叶黄杨氯仿组分通过硅胶柱色谱分离纯化,从中分离得到9个化合物,分别鉴定为:β-谷甾醇(β-Sitosterol,Ⅰ)、豆甾醇(Stigmasterol,Ⅱ)、胡萝卜甙(daucosterol,Ⅲ)、水杨酸(salicylis acid,Ⅳ)、香草酸(vanillicacid,Ⅴ)、5,4′-二羟基-3,3′,7-三甲氧基-黄酮(5,4′-dihydroxy-3,3′,7-trimethoxy-flavone,Ⅵ)、5,4′-二羟基-3,3′,6,7-四甲氧基-黄酮(5,4′-dihydroxy-3,3′,6,7-tetramethoxy-flavone,Ⅶ)、Cleomiscosin A(Ⅷ)、3,5-二羟基-4′,6,7-三甲氧基-黄酮-3′-O-β-D-葡萄糖甙(3,5-dihydroxl-4′,6,7-trimethoxyl-flavone-3′-O-β-D-glucopyranoside,Ⅸ),其中化合物Ⅳ、Ⅴ、Ⅵ、Ⅶ均首次从该属植物中分离得到。     

Chemical Constituents of Glycyrrhiza eurycarpa P. C. Li (1I)

Huang Gancao (Glycyrrhiza eurycarpa P. C. Li) is a new species discovered recently. Its chemical constituents have not been reported before. This paper reports the isolation of ten components (Ⅰ—Ⅹ) from its roots and rhizomes collected in Jinta County, Gansu Province. On the basis of physico-chemlcal constants and spectroscopic (IR, UV, 1HNMR, 13CNMR, MS, and CD) analysis, they were identified as ononin(Ⅰ), liquiritigenin 4 -O-β-D-apio-D-franosyl-(1 →2)-β-D-glucopyranoside(II), isoliquiritigenin-4-O-β-D-apio-D-franosyl-(1→ 2) β-D-glucopyranoside (Ⅲ), choerospondin (Ⅳ), violanthin (Ⅴ), isoviolan thin (Ⅵ), vicenin-2(Ⅶ), uralsaponin A(Ⅷ), daucosterol (Ⅸ), and phloretic acid (Ⅹ), respectively All of them were isolated from this plant for the first time. Choerospondin, violanthin, daucosteroi and phloretic acid were not found in this genus before.     

Studies on the Chemical Constituents of Pyrus Communis

An aqueous extract of the twigs of Pyrus communis L. var. sativa (DC) DC. showed inhibitory effect against S-180. Compounds were isolated from the twigs of P. communis and eight of them were identified as nonacosane (Ⅰ), lupeol (Ⅲ), β-sitosterol (Ⅳ), betulin (Ⅴ), betulinic acid (Ⅵ), daucosterol (Ⅶ), hydroquinone (Ⅷ) and arbutin (Ⅸ) by MS, NMR, IR, UV and some chemical techniques. It was showed that the compounds Ⅲ, Ⅳ, Ⅴ, Ⅵ, Ⅷ and Ⅸ possessed some bacteriostatic activities on Escherichia coli, Salmonella typhi, Shigclla flexneri and Staphylococus aureus. Primary pharmacology tests showed that Ⅷ had inhibitory effect against S-180 (47.5%).     

Chemical Constituents in Inula salsoloides (Turcz.) Ostenf.

Nine known compounds, taraxasterol(Ⅰ), taraxasteryl acetate (Ⅱ), taraxasteryl palmitate (Ⅲ), daucosterol (Ⅳ), luteolin(Ⅴ), stigmastanol(Ⅵ), β-sitosterol(Ⅶ), melissic acid(Ⅷ) and eupatolide(Ⅳ), were isolated from lnula salsoloides for the first time. Their structures were determined from spectra date and chemical evidence. (Ⅲ) and (Ⅸ) exhibited cytotoxic effects.     

Discovery, identification and comparative analysis of non-specific lipid transfer protein (nsLtp) family in Solanaceae

Plant non-specific lipid transfer proteins(nsLtps) have been reported to be involved in plant defense activity against bacterial and fungal pathogens.In this study,we identified 135 (122 putative and 13 previously identified) Solanaceae nsLtps,which are clustered into 8 different groups.By comparing with Boutrot's nsLtp classification,we classified these eight groups into five types (Ⅰ,Ⅱ,Ⅳ,Ⅸ and Ⅹ).We compared Solanaceae nsLtps with Arabidopsis and Gramineae nsLtps and found that (1) Types Ⅰ,Ⅱ and Ⅳ are shared by Solanaceae,Gramineae and Arabidopsis;(2) Types Ⅲ,Ⅴ,Ⅵ and Ⅷ are shared by Gramineae and Arabidopsis but not detected in Solanaceae so far;(3) Type Ⅶ is only found in Gramineae whereas type Ⅸ is present only in Arabidopsis and Solanaceae;(4) Type X is a new type that accounts for 52.59% Solanaceae nsLtps in our data,and has not been reported in any other plant so far.We further built and compared the three-dimensional structures of the eight groups,and found that the major functional diversification within the nsLtp family could be predated to the monocot/dicot divergence,and many gene duplications and sequence variations had happened in the nsLtp family after the monocot/dicot divergence,especially in Solanaceae.     

灰旱獭年龄鉴定的方法

有关灰旱獭Marmota baibacina的年龄鉴定,等(1973)曾以臼齿磨损度作过描述,并划分出6个年龄组,但缺少年龄与个体发育关系的资料。国内以牙齿磨损度鉴定灰旱獭年龄与种群年龄组成尚未见报道。 灰旱獭为天山山地的优势鼠种,分布广,数量多,既是自然疫源性疾病的宿主动物,又是重要的毛皮兽。我们于1983年6-8月对该旱獭的年龄鉴定方法及其年龄组成进行了研究,现将结果报道如下。     

Studies on Alkaloids of Cephalotaxus sinensis (Rehd et Will.)Li

Seven alkaloids were isolated from Cephalotaxus sinensis (Rehd. et Wils.) Li and were identified by elements analysis, melting point and spectral data. Six of them are known alkaloids: cephalotaxine (Ⅰ), Ⅱ-hydroxyeephalotaxine (Ⅱ), drupaeine (Ⅲ), demethylcephalotaxinone (Ⅳ), c-3epi-witsonine (Ⅴ) and wilsonine (Ⅵ). Cephalo- taxine is the major alkaloid of this plant. (Ⅰ–Ⅳ) are alkaloid of Cephalotaxus type and another two (Ⅴ–Ⅵ) are alkaloid of homoerythrina type. (Ⅶ) seems to be a new alkaloid. Its strueture will be studied further.     

The Chemical Principles from the Female lnftorescences of Typha angustata

Seven crystalline compounds have been isolated from the female inflorescences of Typha angustata Bory et Chaub. They were identified as vanillic acid (Ⅰ), E-p-hydroxy- cinnamic acid (Ⅱ), Protocatechuic acid (Ⅲ), E-Pro-penoic acid-3- (4-hydroxyphenyl)- 2,3-dihydrohypropyl ester (Ⅳ), Succinic acid (Ⅴ), p-hydroxybenzaldehyde (Ⅵ) and D-mannitol (Ⅶ). (Ⅳ) is a new compound. (Ⅰ), (Ⅱ), (Ⅲ) and (Ⅶ) were attained for the first time from Typhaceae.     

Study on Chemical Constituents of Gentianopsis barbata var. Stennocalyx H. W. Li ex T.N. Ho

Nine compounds were isolated from Gentianopsis barbata var. stennocalyx H. W. Li ex. T.N.Ho. Their structures are identified as 1-hydroxy4, 7, 8-trimethoxyxanthone (Ⅰ), 1, 7-dihydroxy-3, 8-dimethoxyxanthone (Ⅱ), 1, 7, 8-trihydroxy-3-methoxyxanthone (Ⅲ), 1-O-(β-D- xylopyranosyl-(1→6)-β-D-glucopyranosyl)-3, 7, 8-trimethoxyxanthone (Ⅳ), 1-O-(β-D-xylopy- ranosyl- (1→6)-β-D-glucopyranosyl)-7-hydroxy-3, 8-dimethoxyxanthone (Ⅴ), 1-O-(β-D-xylo- pyranosyl-(1→6)-β-D-glucopyranosyl)-7, 8-dihydroxy-3-methoxyxanthone (Ⅵ), luteolin-7-O- β-D-glucoside (Ⅶ), oleanolic acid (Ⅷ) and ursolic acid (Ⅸ) by means of chemical methods and UV, IR and NMR determinations respectively.     

Three New Xanthones from Halenia elliptica D. Don

Previously, we obtained five xanthoncs from Halenia elliptica D. Don. Recently, other five xanthones have been obtained from the same plants. Among them three are new natural products and their structures are identified as 1, 7-dihydroxy-2, 3, 4, 5-tetramethoxyxanthone (Ⅵ), 1, 5-dihydroxy-2, 3, 7-trimethoxyxanthonc (Ⅶ) and 1, 2-dihydroxy-3, 4, 5-trimethoxyxanthone (Ⅹ). Other two have been known as 1, 5-dihydroxy-2, 3-dimethoxy-xanthone (Ⅷ) and 1, 7-dihydroxy-2, 3-dimethoxyxanthone (Ⅸ).     

Biotransformation of 3b-Hydroxyandrost-5-en-17-one by Cell Suspension Cultures of Catharanthus roseus

Catharanthus roseus (L.) G. Don cell suspension cultures were used to transform 3b-hydroxyandrost-5-en-17-one, the products were isolated by chromatographic methods. Their structures were established by means of NMR and MS spectral analyses. Nine metabolites were respectively elucidated as: androst-4-ene-3,17-dione (Ⅰ), 6a-hydroxyandrost-4-ene-3,17-dione (Ⅱ), 6a,17b-dihydroxyandrost-4-en-3-one (Ⅲ), 6b-hydroxyandrost-4-ene-3,17-dione (Ⅳ), 17b-hydroxyandrost-4-en-3-one (Ⅴ), 15a,17b-dihydroxyandrost-4-en-3-one (Ⅵ), 15b,17b-dihydroxyandrost-4-en-3-one (Ⅶ), 14a-hydroxyandrost-4-ene-3,17-dione (Ⅷ), 17b-hydroxyandrost-4-ene-3,16-dione (Ⅸ). It is the first time to obtain the above compounds by biotransformation with Catharanthus roseus cell cultures.     

Alkaloids of Aconitum vilmoriniaum var. patentipilum

Seven diterpenoid alkaloids were isolated from Aconitum vilmorinianum var. patentipilum W. T. Wang. Their structures were determined as franchetine(Ⅰ), indaconitine(Ⅱ), yunaconitine(Ⅲ), talatisamine(Ⅳ), chasmaconitine(Ⅴ), crassicauline A (Ⅵ), and bikhaconine(Ⅶ). Last one has not been previously found in nature.